Abstract:
INTRODUCTION:We developed and validated reflex testing rules for the microscopic examination (ME) of body fluids (BFs) on the Sysmex XN-550 (Sysmex Corporation) instrument. METHODS:We assessed the detection limits, precision, linearity, and carryover. To develop the reflex testing rules (derivation arm), we tested 515 samples and then validated the rules using another 507 samples (validation arm). RESULTS:All analytical performances were acceptable, and the carryover was negligible. There was agreement between the automated count and ME of red blood cells (r = .98) and total nucleated cells (TNCs) (r = .98), as well as the differential counts of neutrophils (r = .90) and lymphocytes (r = .84). We developed reflex testing rules: TNCs <10/μL, cell 2/cell 1 ratio ≤0.7, HF-BF cells >7.9/100 white blood cells, LY-X ≥85 or LY-Y ≥90, and eosinophils >2.5%. In the validation arm, implementation of the rules resulted in 126 rule-negative samples (24.9%) that were well correlated between the 2 methods. We propose a new workflow for BF cell analysis based on automated counting. CONCLUSION:The Sysmex XN-550 can be a suitable alternative to ME for BF cell analysis, especially for screening samples and subsequent automatic reporting under the rational use of laboratory-specific rules.
journal_name
Int J Lab Hematoljournal_title
International journal of laboratory hematologyauthors
Cho YU,You E,Jang S,Park CJdoi
10.1111/ijlh.12774subject
Has Abstractpub_date
2018-06-01 00:00:00pages
258-267issue
3eissn
1751-5521issn
1751-553Xjournal_volume
40pub_type
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journal_title:International journal of laboratory hematology
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,实务指引
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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