Abstract:
INTRODUCTION:To study the effect of bortezomib alone or in combination with daunorubicin (DNR) on an mdr1 single-factor drug-resistant leukemia cell line K562/MDR1, a multifactor-resistant cell line K562/A02, a drug-sensitive cell line K562, and primary cells from acute myeloid leukemia patients. METHODS:The cell lines were exposed to bortezomib, DNR, and bortezomib plus DNR, and cell proliferation, cell cycle, apoptosis rate, and expression of MDR1/BCL2 were analyzed. RESULTS:Bortezomib potently inhibited growth and increased the apoptosis rate in the cell lines. In K562/MDR1 and K562/A02, the calcium channel blocker verapamil reduced the 50% inhibitory concentration and apoptosis rate of DNR, a P-gp protein substrate, but not of bortezomib. Bortezomib plus DNR had synergistic effect on antiproliferation (synergistic ratio > 1). Apoptosis was substantially more increased by the combination of two drugs than by bortezomib alone. Bortezomib arrested the cell cycles of three cell lines at the G2/M stage, decreased BCL2 mRNA expression, but did not affect MDR1 mRNA levels. The antiproliferative role of bortezomib was also confirmed in primary leukemia cells. CONCLUSION:Bortezomib is a promising potential therapy for acute leukemia, especially mdr1 drug-resistant leukemia.
journal_name
Int J Lab Hematoljournal_title
International journal of laboratory hematologyauthors
Zheng B,Zhou R,Gong Y,Yang X,Shan Qdoi
10.1111/j.1751-553X.2011.01384.xsubject
Has Abstractpub_date
2012-06-01 00:00:00pages
237-47issue
3eissn
1751-5521issn
1751-553Xjournal_volume
34pub_type
杂志文章abstract:INTRODUCTION:Thrombotic thrombocytopenic purpura (TTP) is a rare life-threatening thrombotic microangiopathy (TMA) characterized by the severe deficiency of ADAMTS13 activity (<10%). Rapid ADAMTS13 testing is crucial for early diagnosis and optimal management of TTP patients and other TMAs. The objective of this study ...
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
pub_type: 临床试验,杂志文章
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章,评审
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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更新日期:2010-02-01 00:00:00
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journal_title:International journal of laboratory hematology
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更新日期:2017-12-01 00:00:00
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章,评审
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更新日期:2013-10-01 00:00:00
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
abstract:INTRODUCTION:The Hematoflow (Beckman Coulter, USA) is a new automated hematology analyzer, which provides a 16-part white blood cell count (WBC) differential. METHODS:We evaluated the differential WBC count performance of the Hematoflow. 101 blood samples from patients were selected for comparison analysis. RESULTS:T...
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