Evaluation of the leukocyte differential on a new automated flow cytometry hematology analyzer.

abstract：BACKGROUND:Iron is essential for hemoglobin synthesis during terminal erythropoiesis. To supply adequate iron the carrier transferrin is required together with transferrin receptor endosomal cycle and normal mitochondrial iron utilization. Iron and iron protein deficiencies result in different types of anemia. Iron-def...

journal_title：International journal of laboratory hematology

authors： Camaschella C,Pagani A,Nai A,Silvestri L

更新日期：2016-05-01 00:00:00

abstract：:The diagnosis of iron deficiency in hospital patients can be difficult in the presence of inflammation. A raised serum transferrin receptor (sTfR) level is useful as a marker of iron deficiency as it is unaffected by inflammation. However, diseases that cause an increase in erythropoietic activity can also result in a...

journal_title：International journal of laboratory hematology

authors： Ong KH,Tan HL,Tam LP,Hawkins RC,Kuperan P

更新日期：2008-12-01 00:00:00

abstract：INTRODUCTION:New generation osmotic gradient ektacytometry has become a powerful procedure for measuring red blood cell deformability and therefore for the diagnosis of red blood cell membrane disorders. In this study, we aim to provide further support to the usefulness of osmotic gradient ektacytometry for the differe...

journal_title：International journal of laboratory hematology

authors： Llaudet-Planas E,Vives-Corrons JL,Rizzuto V,Gómez-Ramírez P,Sevilla Navarro J,Coll Sibina MT,García-Bernal M,Ruiz Llobet A,Badell I,Velasco-Puyó P,Dapena JL,Mañú-Pereira MM

更新日期：2018-02-01 00:00:00

abstract：INTRODUCTION:Venous blood (VB) sampling for complete blood count (CBC) via venipuncture is the basic method for the daily evaluation of hematological patients. However, several issues during this process, such as venipuncture difficulty and repetitive attempts, may cause pain, phlebitis, hematomas, inadequate sampling,...

journal_title：International journal of laboratory hematology

authors： Oudatzis G,Tsagarakis NJ,Paterakis G,Vasileiou P,Xenou E,Maraki P,Pangrati T,Angelopoulou MK,Vassilakopoulos TP,Konstantopoulos K

更新日期：2020-10-01 00:00:00

abstract：INTRODUCTION:Thrombocytopenia-absent radius (TAR) syndrome is a rare autosomal recessive disease. Patients are compound heterozygotes for a loss-of-function allele, which in most cases is a large genomic deletion on chromosome 1q21.1 containing the RBM8A gene, and a noncoding variant located in the 5'UTR (rs139428292) ...

journal_title：International journal of laboratory hematology

authors： Nicchia E,Giordano P,Greco C,De Rocco D,Savoia A

更新日期：2016-08-01 00:00:00

abstract：INTRODUCTION:Variability in FVIII measurement is a recognized problem. There are limited data for samples containing recombinant Factor VIII Fc fusion protein (rFVIIIFc). Many studies use samples for which factor concentrate has been spiked into FVIII deficient plasma in vitro. This approach requires validation. AIM/M...

journal_title：International journal of laboratory hematology

authors： Kitchen S,Jennings I,Makris M,Kitchen DP,Woods TAL,Walker ID

更新日期：2019-04-01 00:00:00

abstract：INTRODUCTION:Participation in external quality assessment (EQA) is central to the maintenance of high-quality laboratory results in patient diagnosis and clinical trials. Laboratories in the TAF112582 DETECTIVE study (ClinicalTrials.gov identifier: NCT01376167) are enrolled in the United Kingdom National Quality Assess...

journal_title：International journal of laboratory hematology

authors： Roper DR,De la Salle B,Soni V,Fletcher K,Green JA

更新日期：2017-06-01 00:00:00

abstract：:This 22-year-old women presented to the ante-natal clinic of this hospital for prenatal screening for beta-thalassemia. Cation exchange high performance liquid chromatography (HPLC) using 'Beta Thalassemia Short Program' on Bio-Rad 'Variant' system revealed HbC value of 81.6%. The CBC showed microcytic hypochromic ane...

journal_title：International journal of laboratory hematology

authors： Kumar S,Rana M,Handoo A,Saxena R,Verma IC,Bhargava M,Sood SK

更新日期：2007-10-01 00:00:00

abstract：INTRODUCTION:Beta globin deletion/duplication analysis may serve as a useful adjunct to sequence analysis. Our purpose was to develop a robust assay for beta globin deletion/duplication analysis and determine its role in evaluating patients with beta thalassemia. METHODS:A single tube semi-quantitative fluorescent PCR...

journal_title：International journal of laboratory hematology

authors： Mikula M,Buller-Burckle A,Gallivan M,Sun W,Franklin CR,Strom CM

更新日期：2011-06-01 00:00:00

abstract：:In the 8 years since the publication of the 4th edition of the WHO Classification of Hematopoietic and Lymphoid Tumours in 2008, there has been rapid accumulation of knowledge in the molecular genetics, biology, clinical behavior of many hematologic malignancies. Concepts and models have advanced to the point at which...

journal_title：International journal of laboratory hematology

authors： Hsi ED

更新日期：2017-05-01 00:00:00

abstract：INTRODUCTION:Presence of schistocytes in peripheral blood smear supporting haemolysis is important for diagnosis and decision-making in paediatric haemolytic uraemic syndrome (HUS). High observer dependency and requirement of expertise for peripheral smear evaluation propels us to think of other modalities to overcome ...

journal_title：International journal of laboratory hematology

authors： Govindarajan S,Bhatia P,Dawman L,Tiewsoh K

更新日期：2021-02-01 00:00:00

abstract：INTRODUCTION:Immature platelet fraction (IPF) is a parameter for reticulated platelets. A high percentage IPF (%-IPF) is indicative of consumptive or recovering thrombocytopenic disorders in contrast to a low %-IPF seen in aplastic states. Absolute IPF (A-IPF) specifically reflects the number of immature platelets in c...

journal_title：International journal of laboratory hematology

authors： Ko YJ,Kim H,Hur M,Choi SG,Moon HW,Yun YM,Hong SN

更新日期：2013-10-01 00:00:00

abstract：:The performance of factor VII (FVII) assays currently used by clinical laboratories was examined in North American Specialized Coagulation Laboratory Association (NASCOLA) proficiency tests. Data from 12 surveys conducted between 2008 and 2010, involving 20 unique specimens plus four repeat-tested specimens, were anal...

journal_title：International journal of laboratory hematology

authors： Zantek ND,Hsu P,Refaai MA,Ledford-Kraemer M,Meijer P,Van Cott EM

更新日期：2013-06-01 00:00:00

abstract：INTRODUCTION:Autoimmune haemolytic anaemia (AIHA) is defined as the increased destruction of red blood cells (RBCs) in the presence of anti-RBC autoantibodies and/or complement. Its pathogenesis is multifactorial and includes changes in mechanisms of cytokine production and functionality. A number of recent studies hav...

journal_title：International journal of laboratory hematology

authors： D'Abronzo LS,Barros MM,Bordin JO,Figueiredo MS

更新日期：2012-08-01 00:00:00

abstract：INTRODUCTION:While the presence of disseminated intravascular coagulation (DIC) has been implicated in worse clinical outcome in acute leukemia, the relationship between different subtypes of acute leukemia and the clinicopathologic features of DIC has not been systematically well studied. METHODS:In this study, we re...

journal_title：International journal of laboratory hematology

authors： Shahmarvand N,Oak JS,Cascio MJ,Alcasid M,Goodman E,Medeiros BC,Arber DA,Zehnder JL,Ohgami RS

更新日期：2017-08-01 00:00:00

abstract：INTRODUCTION:Malaria is a life-threatening infectious disease, which has been for long confined to specific endemic areas. Nevertheless, the recent increase in immigration flows from endemic regions and imported cases has reemphasized many diagnostic challenges in Western countries, thus paving the way to introduce rap...

journal_title：International journal of laboratory hematology

authors： Buoro S,Manenti B,Seghezzi M,Moioli V,Bagorria M,Callegaro A,Ottomano C,Lippi G

更新日期：2018-06-01 00:00:00

abstract：INTRODUCTION:These recommendations are intended to develop a consensus in the previously published papers as to which parameters and what values should be considered critical. A practical guide on the standardization of critical results management in haematology laboratories would be beneficial as part of good laborato...

journal_title：International journal of laboratory hematology

authors： Keng TB,De La Salle B,Bourner G,Merino A,Han JY,Kawai Y,Peng MT,McCafferty R,International Council for Standardization in Haematology (ICSH).

更新日期：2016-10-01 00:00:00

abstract：:Eosinophilia is typically secondary, that is, reactive, in nature and is associated with a wide variety of neoplastic and non-neoplastic disorders. Clonal eosinophilia is also seen in a wide variety of hematopoietic neoplasms, and sub-classification can be diagnostically challenging. A proper evaluation of persistent ...

journal_title：International journal of laboratory hematology

authors： Larsen RL,Savage NM

更新日期：2019-04-01 00:00:00

abstract：INTRODUCTION:Several factors influence the severity of Plasmodium falciparum; here, we investigate the impact of alpha+-thalassaemia genotype on P. falciparum parasitemia and prevalence of severe anaemia amongst microcytic children from Kumasi, Ghana. METHODS:Seven hundred and thirty-two children (≤10 years) with P. f...

journal_title：International journal of laboratory hematology

authors： Opoku-Okrah C,Gordge M,Kweku Nakua E,Abgenyega T,Parry M,Robertson C,Smith CL

更新日期：2014-02-01 00:00:00

abstract：INTRODUCTION:Frequent PT (INR) testing may represent a problem for patients on warfarin treatment, and capillary or small-volume tubes may be more appropriate for such patients. A demand for small-volume tubes also comes from pediatric wards. Yet, while various small-volume tubes are available, they have not been prope...

journal_title：International journal of laboratory hematology

authors： Onelöv L,Basmaji R,Svensson A,Nilsson M,Antovic JP

更新日期：2015-10-01 00:00:00

abstract：:Acquired haemophilia is a rare phenomenon and prompt diagnosis is essential for successful treatment. Early laboratory detection could minimize its potentially devastating consequences and reduce mortality but when a masking element such as anticoagulant therapy is present, delay in diagnosis is not uncommon. A prolon...

journal_title：International journal of laboratory hematology

authors： Vadikolia CM,Riddell A,Brooks S,Yee TT,Brown S,Lee C

更新日期：2007-02-01 00:00:00

abstract：INTRODUCTION:Cellular analysis in body fluids (BFs) provides important diagnostic information in various pathological settings. This study was hence aimed at comparing automated cell count obtained with Mindray BC-6800 (BC-BF) vs Sysmex XN-series (XN-BF) and evaluating other quantitative and qualitative information pro...

journal_title：International journal of laboratory hematology

authors： Buoro S,Seghezzi M,Dominoni P,Moioli V,Manenti B,Previtali G,Ottomano C,Lippi G

更新日期：2019-04-01 00:00:00

abstract：INTRODUCTION:To measure direct factor Xa inhibitor (apixaban, edoxaban, rivaroxaban) concentrations, dedicated chromogenic anti-Xa assays are recommended as suitable methods to provide rapid drug quantification. Moreover, the high-performance liquid chromatography with ultraviolet detection (HPLC-UV) is reported as a r...

journal_title：International journal of laboratory hematology

authors： Cini M,Legnani C,Padrini R,Cosmi B,Dellanoce C,De Rosa G,Marcucci R,Pengo V,Poli D,Testa S,Palareti G

更新日期：2020-04-01 00:00:00

abstract：INTRODUCTION:The STA R Max® 2 is a new coagulation analyser developed by Diagnostica Stago, able to perform clotting, chromogenic and immuno-turbidimetric tests. A pre-analytical module build into the cap-piercing needle performs the sample integrity verification (sample tube filling and measurement of haemolysis, icte...

journal_title：International journal of laboratory hematology

authors： Cupaiolo R,Govaerts D,Blauwaert M,Cauchie P

更新日期：2019-12-01 00:00:00

abstract：:The majority, if not all, of human cell types secrete extracellular vesicles (EVs) into their environment, at least partly as a means of intercellular communication. These secreted vesicles can be detected in most bodily fluids including blood, urine, and saliva. The number of secreted vesicles and their composition i...

journal_title：International journal of laboratory hematology

authors： van der Meel R,Krawczyk-Durka M,van Solinge WW,Schiffelers RM

更新日期：2014-06-01 00:00:00

abstract：:Modern photo-optical coagulometers collect optical data in the form of reaction curves and offer a possibility to determine clotting times at different points of clot formation by using different evaluation modes. The objectives of this study were to determine the possible impact of an evaluation mode on activated par...

journal_title：International journal of laboratory hematology

authors： Milos M,Herak DC,Zadro R

更新日期：2010-02-01 00:00:00

abstract：:Prediction of the timing of platelet recovery after chemotherapy and hematopoietic stem cell transplantation (HSCT) allows for optimal platelet transfusion. We assessed the clinical utility of the percentage value of the immature platelet fraction (IPF%) monitored using an XE-2100 automated hematology analyzer to pred...

journal_title：International journal of laboratory hematology

authors： Yamaoka G,Kubota Y,Nomura T,Inage T,Arai T,Kitanaka A,Saigo K,Iseki K,Baba N,Taminato T

更新日期：2010-12-01 00:00:00

abstract：INTRODUCTION:The IgV(H) mutational status of B-cell chronic lymphocytic leukemia (B-CLL) is of prognostic value. Expression of ZAP-70 in B-CLL is a surrogate marker for IgV(H) unmutated (UM). As determination of IgV(H) mutational status involves a methodology currently unavailable for most clinical laboratories, it is ...

journal_title：International journal of laboratory hematology

authors： Marquez ME,Deglesne PA,Suarez G,Romano E

更新日期：2011-04-01 00:00:00

abstract：INTRODUCTION:The workflow in clinical flow cytometry laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. Using the Beckman Coulter dry coating technology, we customized a ten-color tube with dried antibody reagents, designated the Duraclone sc...

journal_title：International journal of laboratory hematology

authors： Correia RP,Rajab A,Bento LC,Alexandre AM,Vaz AC,Schimidell D,Pedro EC,Perin FS,Nozawa ST,Barroso RS,Bacal NS

更新日期：2018-04-01 00:00:00

abstract：INTRODUCTION:Aberrant activation of Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway leads to deregulated proliferation, differentiation, or apoptosis of normal cells. One of the members of this family, JAK2, plays a very important role in metabolizing carcinogens and medications. This...

journal_title：International journal of laboratory hematology

authors： Zhong Y,Wu J,Ma R,Cao H,Wang Z,Ding J,Cheng L,Feng J,Chen B

更新日期：2012-06-01 00:00:00