Abstract:
INTRODUCTION:It is widely recognized that plasma cells (PCs) are under-represented in flow cytometry (FC) studies, but the causes of this phenomenon are poorly understood. We sought to study potential variables that affect PC recovery by flow cytometry (FC) in the analysis of plasma cell myeloma (PCM). METHODS:We retrospectively performed PC differential counts and morphologic assessment on PCM peripheral blood (PB) smears, bone marrow (BM) aspirate smears and posterythrocyte lysis cytospins. PCs were enumerated by FC, excluding erythroid events/debris, and were defined as CD38(bright+), CD45(dim to negative) events. PC recovery was calculated as follows: cytospin/aspirate, FC/aspirate, and FC/cytospin. RESULTS:Sixty-four BM analyses from 42 patients showed a mean aspirate PC% of 32.9 ± 23.2%. The mean PC% decreased in both the cytospin (10.9%) and by FC (8.2%). The difference between PC% in the cytospin and by FC was statistically significant (P < 0.03). Mature PC morphology and lower aspirate PC% had poorer recovery (P < 0.05) but higher-risk cytogenetics (deletions of 13q and TP53) was associated with increased PC recovery. Immunophenotype, heavy chain type, and treatment did not affect PC recovery. PB specimens had superior recovery compared with BM samples. CONCLUSIONS:Similar to prior reports, the greatest loss of PC in BM evaluation occurs between the aspirate and postlysis specimens; however, a small amount occurs from further processing. Additional morphologic and cytogenetic factors also appear to influence recovery in addition to overall PC%.
journal_name
Int J Lab Hematoljournal_title
International journal of laboratory hematologyauthors
Cogbill CH,Spears MD,Vantuinen P,Harrington AM,Olteanu H,Kroft SHdoi
10.1111/ijlh.12411subject
Has Abstractpub_date
2015-12-01 00:00:00pages
797-808issue
6eissn
1751-5521issn
1751-553Xjournal_volume
37pub_type
杂志文章abstract:INTRODUCTION:Participation in external quality assessment (EQA) is central to the maintenance of high-quality laboratory results in patient diagnosis and clinical trials. Laboratories in the TAF112582 DETECTIVE study (ClinicalTrials.gov identifier: NCT01376167) are enrolled in the United Kingdom National Quality Assess...
journal_title:International journal of laboratory hematology
pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2017-06-01 00:00:00
abstract:INTRODUCTION:These recommendations are intended to develop a consensus in the previously published papers as to which parameters and what values should be considered critical. A practical guide on the standardization of critical results management in haematology laboratories would be beneficial as part of good laborato...
journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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abstract:INTRODUCTION:The Sysmex XN-series is a new automated hematology analyzer designed to improve the accuracy of cell counts and the specificity of the flagging events. METHODS:The basic characteristics and the performance of new measurement channels of the XN were evaluated and compared with the Sysmex XE-2100 and the ma...
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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journal_title:International journal of laboratory hematology
pub_type: 临床试验,杂志文章
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journal_title:International journal of laboratory hematology
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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pub_type: 杂志文章,实务指引
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journal_title:International journal of laboratory hematology
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journal_title:International journal of laboratory hematology
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pub_type: 杂志文章
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pub_type: 杂志文章
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