Abstract：
:Etomoxir (ETO) is a widely used small-molecule inhibitor of fatty acid oxidation (FAO) through its irreversible inhibitory effects on the carnitine palmitoyl-transferase 1a (CPT1a). We used this compound to evaluate the role of fatty acid oxidation in rapidly proliferating T cells following costimulation through the CD28 receptor. We show that ETO has a moderate effect on T cell proliferation with no observable effect on memory differentiation, but a marked effect on oxidative metabolism. We show that this oxidative metabolism is primarily dependent upon glutamine rather than FAO. Using an shRNA approach to reduce CPT1a in T cells, we further demonstrate that the inhibition of oxidative metabolism in T cells by ETO is independent of its effects on FAO at concentrations exceeding 5 μM. Concentrations of ETO above 5 μM induce acute production of ROS with associated evidence of severe oxidative stress in proliferating T cells. In aggregate, these data indicate that ETO lacks specificity for CTP1a above 5 μM, and caution should be used when employing this compound for studies in cells due to its non-specific effects on oxidative metabolism and cellular redox.
journal_name
Sci Repjournal_title
Scientific reportsauthors
O'Connor RS,Guo L,Ghassemi S,Snyder NW,Worth AJ,Weng L,Kam Y,Philipson B,Trefely S,Nunez-Cruz S,Blair IA,June CH,Milone MCdoi
10.1038/s41598-018-24676-6subject
Has Abstractpub_date
2018-04-19 00:00:00pages
6289issue
1issn
2045-2322pii
10.1038/s41598-018-24676-6journal_volume
8pub_type
杂志文章abstract：:The demand for rapid, consistent and easy-to-use techniques for detecting and identifying pathogens in various areas, such as clinical diagnosis, the pharmaceutical industry, environmental science and food inspection, is very important. In this study, the reference strains of six food-borne pathogens, namely, Escheric...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59448-8
更新日期：2020-02-11 00:00:00
abstract：:Endosymbioses are driving forces underlying cell evolution. The endosymbiosis exhibited by Paramecium bursaria is an excellent model with which to study symbiosis. A single-cell microscopic analysis of P. bursaria reveals that endosymbiont numbers double when the host is in the division phase. Consequently, endosymbio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31638
更新日期：2016-08-17 00:00:00
abstract：:The current study aimed to formulate Selenium-Chitosan-Mupirocin (M-SeNPs-CCH) complex. The nanohybrid system was prepared using chitosan-cetyltrimethylammonium bromide (CTAB)-based hydrogel (CCH) that entrapped mupirocin (M) and selenium nanoparticles (SeNPs). The in vitro studies were performed by evaluation of the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59510-5
更新日期：2020-02-18 00:00:00
abstract：:Generating various laser sources is important in the communication systems. We propose an approach that uses a mechanical resonator coupled with the optical fibre system to produce periodic and chaotic optical signals. The resonator is structured in such a way that the nonlinear oscillation occurs conveniently. The me...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05020-w
更新日期：2017-07-06 00:00:00
abstract：:We have recently reported that repeated systemic treatments of extract from Corydalis yanhusuo alleviate neuropathic pain and levo-tetrahydropalmatine (l-THP) is one of active components from Corydalis. We designed this study to investigate antinociceptive effect of l-THP in acute and chronic pain models and related m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37850
更新日期：2016-12-02 00:00:00
abstract：:A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24599
更新日期：2016-04-19 00:00:00
abstract：:Mutations affecting the transcription factor FOXP2 cause a rare form of severe speech and language disorder. Although it is clear that sufficient FOXP2 expression is crucial for normal brain development, little is known about how this transcription factor is regulated. To investigate post-translational mechanisms for ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20911
更新日期：2016-02-12 00:00:00
abstract：:Autosomal dominant North Carolina macular dystrophy (NCMD) is believed to represent a failure of macular development. The disorder has been linked to two loci, MCDR1 (chromosome 6q16) and MCDR3 (chromosome 5p15-p13). Recently, non-coding variants upstream of PRDM13 (MCDR1) and a duplication including IRX1 (MCDR3) have...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06387-6
更新日期：2017-08-08 00:00:00
abstract：:Blood transfusion is fundamental in managing hematologic malignancies. We sought to evaluate the need and availability of blood products for patients with hematological malignancies at Uganda Cancer Institute. We prospectively studied the demand and supply of blood for patients with thrombocytopenia (platelet count ≤5...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59773-y
更新日期：2020-02-18 00:00:00
abstract：:Osteoporosis is the most common age-related bone disease worldwide and is usually clinically asymptomatic until the first fracture happens. MicroRNAs are critical molecular regulators in bone remodelling processes and are stabilised in the blood. The aim of this project was to identify circulatory microRNAs associated...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26525-y
更新日期：2018-05-30 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-43786-3
更新日期：2019-10-28 00:00:00
abstract：:The healthy adult heart primarily relies on fatty acid oxidation (FAO) for energy production but instantaneously adapts its substrate preference in response to physiological or pathological challenges. Accurate FAO measurements are crucial to investigate early metabolic (mal)adaptations. While measurements in intact c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19478-9
更新日期：2018-01-24 00:00:00
abstract：:The triatomine Rhodnius prolixus, a vector of the etiological agent of Chagas disease, has long been used as model to understand important aspects of insect physiology. Despite this history, the impact of the nutritional state on regulatory pathways associated with reproductive performance in triatomines has never bee...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67932-4
更新日期：2020-07-10 00:00:00
abstract：:A 3D cell culture is preferred to 2D cell culture since it allows cells to grow in all directions in vitro, similar to how they would in vivo. 3D cell culture plates currently used in tissue engineering research have limited access to control the geometry. Furthermore, 3D cell culture plate manufacturing methods are r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50186-0
更新日期：2019-09-27 00:00:00
abstract：:Potato (Solanum tuberosum L.) is the fourth most important crop worldwide. Potato virus A (PVA) is one of the most harmful viruses infecting potatoes. However, the molecular mechanisms governing the responses to PVA infection in potato at the transcriptional and post-transcriptional levels are not well understood. In ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17059-w
更新日期：2017-12-05 00:00:00
abstract：:The spread of multidrug-resistant Staphylococcus aureus strains, including methicillin-resistant S. aureus (MRSA), has shortened the useful life of anti-staphylococcal drugs enormously. Two approaches can be followed to address this problem: screening various sources for new leads for antibiotics or finding ways to di...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03716-7
更新日期：2017-06-19 00:00:00
abstract：:The LiBH4/CaH2 composite are firstly studied as Concentrating Solar Power Thermal Storage Material. The LiBH4/CaH2 composite according to the stoichiometric ratio are synthesized by high-energy ball milling method. The kinetics, thermodynamics and cycling stability of LiBH4/CaH2 composite are investigated by XRD (X-ra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41754
更新日期：2017-01-31 00:00:00
abstract：:Plant health is strongly connected with plants´ microbiome. In case of raw-eaten plants, the microbiome can also affect human health. To study potential impacts on health issues of both hosts, the microbiome composition of seven different Brassica vegetables, originating from different food processing pathways, was an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17949-z
更新日期：2017-12-15 00:00:00
abstract：:Understanding of lipid oxidation mechanisms (e.g., auto-oxidation and photo-oxidation) in foods and cosmetics is deemed essential to maintain the quality of such products. In this study, the oxidation mechanisms in foods and cosmetics were evaluated through analysis of linoleic acid hydroperoxide (LAOOH) and linoleic ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43645-1
更新日期：2019-05-14 00:00:00
abstract：:Parthenium hysterophorus exhibits tolerance to a great extent against abiotic stresses including high light intensities. In this study, P. hysterophorus was subjected to three different light intensities viz. control (CL, 250 µmol photons m-2 s-1), moderately high (ML, 500 µmol photons m-2 s-1) and high (HL, 1000 µmol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65721-7
更新日期：2020-06-02 00:00:00
abstract：:We have proposed and successfully demonstrated a novel approach to direct conversion of mechanical energy into electrical energy using microfluidics. The method combines previously demonstrated reverse electrowetting on dielectric (REWOD) phenomenon with the fast self-oscillating process of bubble growth and collapse....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16537
更新日期：2015-11-16 00:00:00
abstract：:Phase estimation represents a crucial challenge in many fields of Physics, ranging from Quantum Metrology to Quantum Information Processing. This task is usually pursued by means of interferometric schemes, in which the choice of the input states and of the detection apparatus is aimed at minimizing the uncertainty in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26025
更新日期：2016-05-18 00:00:00
abstract：:The vertical migration of zooplankton and micronekton (hereafter 'zooplankton') has ramifications throughout the food web. Here, we present the first evidence that climate fluctuations affect the vertical migration of zooplankton in the Southern Ocean, based on multi-year acoustic backscatter data from one of the deep...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46423-1
更新日期：2019-07-12 00:00:00
abstract：:We study semiconductor hyperbolic metamaterials (SHMs) at the quantum limit experimentally using spectroscopic ellipsometry as well as theoretically using a new microscopic theory. The theory is a combination of microscopic density matrix approach for the material response and Green's function approach for the propaga...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35099-8
更新日期：2018-11-12 00:00:00
abstract：:We investigate the behaviour of the recently proposed Quantum PageRank algorithm, in large complex networks. We find that the algorithm is able to univocally reveal the underlying topology of the network and to identify and order the most relevant nodes. Furthermore, it is capable to clearly highlight the structure of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02773
更新日期：2013-10-04 00:00:00
abstract：:An in-depth focused study of specific cases of patients with recurrent thrombosis may help to identify novel circumstances, genetic and acquired factors contributing to the development of this disorder. The aim of this study was to carry out a detailed and sequential analysis of samples from a patient suffering from e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44556
更新日期：2017-03-17 00:00:00
abstract：:Parasites exhibiting a high degree of host specificity are expected to be intimately associated with their hosts. Therefore, the evolution of host-specific parasites is at least partially shaped by the evolutionary history and distribution of such hosts. Gill ectoparasites of Dactylogyrus (Monogenea) are specific to c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31382-w
更新日期：2018-08-29 00:00:00
abstract：:Transcriptional coactivator PGC-1α and its splice variant NT-PGC-1α play crucial roles in regulating cold-induced thermogenesis in brown adipose tissue (BAT). PGC-1α and NT-PGC-1α are highly induced by cold in BAT and subsequently bind to and coactivate many transcription factors to regulate expression of genes involv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26244-4
更新日期：2018-05-18 00:00:00
abstract：:Linearly polarized microwave radiation is shown to have its plane of polarization converted to the orthogonal state upon reflection from an ultrathin (λ/25) cavity array. The structure benefits from an uncomplicated design consisting of a metallic grating closely separated from a ground plane by a dielectric spacer. A...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09366
更新日期：2015-03-23 00:00:00
abstract：:X-ray free-electron lasers (XFELs) provide extremely bright and highly spatially coherent x-ray radiation with femtosecond pulse duration. Currently, they are widely used in biology and material science. Knowledge of the XFEL statistical properties during an experiment may be vitally important for the accurate interpr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19793-1
更新日期：2018-02-02 00:00:00
