当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Involvement of AMP-activated Protein Kinase (AMPK) in Regulation of Cell Membrane Potential in a Gastric Cancer Cell Line.

Involvement of AMP-activated Protein Kinase (AMPK) in Regulation of Cell Membrane Potential in a Gastric Cancer Cell Line.

Abstract：

:Membrane potential (Vmem) is a key bioelectric property of non-excitable cells that plays important roles in regulating cell proliferation. However, the regulation of Vmem itself remains largely unexplored. We found that, under nutrient starvation, during which cell division is inhibited, MKN45 gastric cancer cells were in a hyperpolarized state associated with a high intracellular chloride concentration. AMP-activated protein kinase (AMPK) activity increased, and expression of cystic fibrosis transmembrane conductance regulator (CFTR) decreased, in nutrient-starved cells. Furthermore, the increase in intracellular chloride concentration level and Vmem hyperpolarization in nutrient-starved cells was suppressed by inhibition of AMPK activity. Intracellular chloride concentrations and hyperpolarization increased after over-activation of AMPK using the specific activator AICAR or suppression of CFTR activity using specific inhibitor GlyH-101. Under these conditions, proliferation of MKN45 cells was inhibited. These results reveal that AMPK controls the dynamic change in Vmem by regulating CFTR and influencing the intracellular chloride concentration, which in turn influences cell-cycle progression. These findings offer new insights into the mechanisms underlying cell-cycle arrest regulated by AMPK and CFTR.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Zhu L,Yu XJ,Xing S,Jin F,Yang WJ

doi

10.1038/s41598-018-24460-6

subject

Has Abstract

pub_date

2018-04-16 00:00:00

pages

6028

issue

1

issn

2045-2322

pii

10.1038/s41598-018-24460-6

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Ultrathin Air-Stable n-Type Organic Phototransistor Array for Conformal Optoelectronics.

    abstract：:Development of conformal n-channel organic phototransistor (OPT) array is urgent for future applications of organic complementary circuits in portable and wearable electronics and optoelectronics. In this work, the ultrathin conformal OPT array based on air-stable n-type PTCDI-C13H27 was fabricated. The OPT array show...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-35062-7

    authors： Liu M,Wang H,Tang Q,Zhao X,Tong Y,Liu Y

    更新日期：2018-11-09 00:00:00

  • Changes in land use alter soil quality and aggregate stability in the highlands of northern Ethiopia.

    abstract：:Land use change alters biodiversity and soil quality and thus affects ecosystem functions. This study investigated the effects of changes in land use on major soil quality indicators. Soil samples were taken from a depth of 0-10 cm (top soil) under four major land uses (cropland, grassland, area exclosure, eucalyptus ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14128-y

    authors： Delelegn YT,Purahong W,Blazevic A,Yitaferu B,Wubet T,Göransson H,Godbold DL

    更新日期：2017-10-19 00:00:00

  • Overcoming challenges in human saliva gene expression measurements.

    abstract：:Saliva, as a non-invasive and easily accessible biofluid, has been shown to contain RNA biomarkers for prediction and diagnosis of several diseases. However, systematic analysis done by our group identified two problematic issues not coherently described before: (1) most of the isolated RNA originates from the oral mi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-67825-6

    authors： Ostheim P,Tichý A,Sirak I,Davidkova M,Stastna MM,Kultova G,Paunesku T,Woloschak G,Majewski M,Port M,Abend M

    更新日期：2020-07-07 00:00:00

  • Dirac gap opening and Dirac-fermion-mediated magnetic coupling in antiferromagnetic Gd-doped topological insulators and their manipulation by synchrotron radiation.

    abstract：:A new kind of magnetically-doped antiferromagnetic (AFM) topological insulators (TIs) with stoichiometry Bi1.09Gd0.06Sb0.85Te3 has been studied by angle-resolved photoemission spectroscopy (ARPES), superconducting magnetometry (SQUID) and X-ray magnetic circular dichroism (XMCD) with analysis of its electronic structu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41137-w

    authors： Shikin AM,Estyunin DA,Surnin YI,Koroleva AV,Shevchenko EV,Kokh KA,Tereshchenko OE,Kumar S,Schwier EF,Shimada K,Yoshikawa T,Saitoh Y,Takeda Y,Kimura A

    更新日期：2019-03-18 00:00:00

  • The impact of acute stress on hormones and cytokines, and how their recovery is affected by music-evoked positive mood.

    abstract：:Stress and recovery from stress significantly affect interactions between the central nervous system, endocrine pathways, and the immune system. However, the influence of acute stress on circulating immune-endocrine mediators in humans is not well known. Using a double-blind, randomized study design, we administered a...

    journal_title：Scientific reports

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/srep23008

    authors： Koelsch S,Boehlig A,Hohenadel M,Nitsche I,Bauer K,Sack U

    更新日期：2016-03-29 00:00:00

  • Enhanced high-frequency absorption of anisotropic Fe3O4/graphene nanocomposites.

    abstract：:Anisotropic Fe3O4 nanoparticle and a series of its graphene composites have been successfully prepared as high-frequency absorbers. The crystal structure, morphology and magnetic property of the samples were detailed characterized through X-ray diffractometer (XRD), transmission electron microscopy (TEM) and vibrating...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep25075

    authors： Yin Y,Zeng M,Liu J,Tang W,Dong H,Xia R,Yu R

    更新日期：2016-05-04 00:00:00

  • Tobacco smoke and morphine alter peripheral and CNS inflammation following HIV infection in a humanized mouse model.

    abstract：:Tobacco smoking is common in HIV-infected patients, and is prevalent among intravenous opiate abusers. Conversely, intravenous opiate abusers are more likely HIV-infected, and opiate abuse is associated with more severe neuroinflammation. Given the coincident use of tobacco smoking among HIV-infected intravenous drug ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-70374-7

    authors： Cornwell WD,Sriram U,Seliga A,Zuluaga-Ramirez V,Gajghate S,Rom S,Winfield M,Heldt NA,Ambrose D,Rogers TJ,Persidsky Y

    更新日期：2020-08-19 00:00:00

  • HDNetDB: A Molecular Interaction Database for Network-Oriented Investigations into Huntington's Disease.

    abstract：:Huntington's disease (HD) is a progressive and fatal neurodegenerative disorder caused by an expanded CAG repeat in the huntingtin gene. Although HD is monogenic, its molecular manifestation appears highly complex and involves multiple cellular processes. The recent application of high throughput platforms such as mic...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-05224-0

    authors： Kalathur RKR,Pedro Pinto J,Sahoo B,Chaurasia G,Futschik ME

    更新日期：2017-07-12 00:00:00

  • The natural retinoprotectant chrysophanol attenuated photoreceptor cell apoptosis in an N-methyl-N-nitrosourea-induced mouse model of retinal degenaration.

    abstract：:Retinitis pigmentosa (RP) is an inherited photoreceptor-degenerative disease, and neuronal degeneration in RP is exacerbated by glial activation. Cassia seed (Jue-ming-zi) is a traditional herbal medicine commonly used to treat ocular diseases in Asia. In this report, we investigated the retina-protective effect of ch...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41086

    authors： Lin FL,Lin CH,Ho JD,Yen JL,Chang HM,Chiou GC,Cheng YW,Hsiao G

    更新日期：2017-01-23 00:00:00

  • Author Correction: Signal quality of simultaneously recorded endovascular, subdural and epidural signals are comparable.

    abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-018-36257-8

    authors： John SE,Opie NL,Wong YT,Rind GS,Ronayne SM,Gerboni G,Bauquier SH,O'Brien TJ,May CN,Grayden DB,Oxley TJ

    更新日期：2018-11-27 00:00:00

  • Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme.

    abstract：:Most biodiesel currently in use consists of fatty acid methyl esters (FAMEs) produced by transesterification of plant oils with methanol. To reduce competition with food supplies, it would be desirable to directly produce biodiesel in microorganisms. To date, the most effective pathway for the production of biodiesel ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep24239

    authors： Sherkhanov S,Korman TP,Clarke SG,Bowie JU

    更新日期：2016-04-07 00:00:00

  • Laminin α2, α4, and α5 Chains Positively Regulate Migration and Survival of Oligodendrocyte Precursor Cells.

    abstract：:In the developing central nervous system (CNS), oligodendrocyte precursor cells (OPCs) migrate along blood vessels and are widely distributed in the CNS. Meanwhile, OPCs require survival factors from the extracellular microenvironment. In other tissues, laminins, heterotrimetric (αβγ) extracellular matrix proteins, pr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56488-7

    authors： Suzuki N,Hyodo M,Hayashi C,Mabuchi Y,Sekimoto K,Onchi C,Sekiguchi K,Akazawa C

    更新日期：2019-12-27 00:00:00

  • Ubiquitin Ligase NEDD4 Regulates PPARγ Stability and Adipocyte Differentiation in 3T3-L1 Cells.

    abstract：:Peroxisome proliferator-activated receptor-γ (PPARγ) is a ligand-activated nuclear receptor which controls lipid and glucose metabolism. It is also the master regulator of adipogenesis. In adipocytes, ligand-dependent PPARγ activation is associated with proteasomal degradation; therefore, regulation of PPARγ degradati...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38550

    authors： Li JJ,Wang R,Lama R,Wang X,Floyd ZE,Park EA,Liao FF

    更新日期：2016-12-05 00:00:00

  • Photodynamic activation as a molecular switch to promote osteoblast cell differentiation via AP-1 activation.

    abstract：:In photodynamic therapy (PDT), cells are impregnated with a photosensitizing agent that is activated by light irradiation, thereby photochemically generating reactive oxygen species (ROS). The amounts of ROS produced depends on the PDT dose and the nature of the photosensitizer. Although high levels of ROS are cytotox...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13114

    authors： Kushibiki T,Tu Y,Abu-Yousif AO,Hasan T

    更新日期：2015-08-17 00:00:00

  • Booster immunizations with DNA plasmids encoding HER-2/neu prevent spontaneous mammary cancer in HER-2/neu transgenic mice over life span.

    abstract：:Cancer vaccines are less effective at old than at young age because of immunosenescence. Besides, in preliminary observations we showed that the immunization with HER-2/neu DNA plasmid in transgenic young mice (standard immunization, SI) delays but not abrogate spontaneous mammary tumours progressively appearing durin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-03286-8

    authors： Provinciali M,Barucca A,Orlando F,Pierpaoli E

    更新日期：2017-06-08 00:00:00

  • Positively interacting strains that co-circulate within a network structured population induce cycling epidemics of Mycoplasma pneumoniae.

    abstract：:Mycoplasma pneumoniae (MP) is considered a common cause of pneumonia, causing about 15-20% of adult community-acquired pneumonia (CAP) and up to 40% of cases in children. It has often been observed that MP epidemics last approximately 1-2 years and occur every 3-7 years, with the dominant strains alternating between e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36325-z

    authors： Zhang XS,Zhao H,Vynnycky E,Chalker V

    更新日期：2019-01-24 00:00:00

  • A Bioactive Chemical Markers Based Strategy for Quality Assessment of Botanical Drugs: Xuesaitong Injection as a Case Study.

    abstract：:Current chemical markers based quality assessment methods largely fail to reflect intrinsic chemical complexity and multiple mechanisms of action of botanical drugs (BD). The development of novel quality markers is greatly needed. Here we propose bioactive chemical markers (BCM), defined as a group of chemo-markers th...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02305-y

    authors： Yang Z,Shao Q,Ge Z,Ai N,Zhao X,Fan X

    更新日期：2017-05-25 00:00:00

  • Phase transitions, mechanical properties and electronic structures of novel boron phases under high-pressure: a first-principles study.

    abstract：:We have explored the mechanical properties, electronic structures and phase transition behaviors of three designed new phases for element boron from ambient condition to high-pressure of 120 GPa including (1) a C2/c symmetric structure (m-B₁₆); (2) a symmetric structure (c-B₅₆) and (3) a Pmna symmetric structure (o-B₂...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep06786

    authors： Fan C,Li J,Wang L

    更新日期：2014-10-27 00:00:00

  • Effects of Atmospheric-Pressure N2, He, Air, and O2 Microplasmas on Mung Bean Seed Germination and Seedling Growth.

    abstract：:Atmospheric-pressure N2, He, air, and O2 microplasma arrays have been used to investigate the effects of plasma treatment on seed germination and seedling growth of mung bean in aqueous solution. Seed germination and growth of mung bean were found to strongly depend on the feed gases used to generate plasma and plasma...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32603

    authors： Zhou R,Zhou R,Zhang X,Zhuang J,Yang S,Bazaka K,Ken Ostrikov K

    更新日期：2016-09-01 00:00:00

  • Somatic and visceral effects of word valence, arousal and concreteness in a continuum lexical space.

    abstract：:Although affective and semantic word properties are known to independently influence our sensorimotor system, less is known about their interaction. We investigated this issue applying a data-driven mixed-effects regression approach, evaluating the impact of lexical-semantic properties on electrophysiological paramete...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56382-2

    authors： Vergallito A,Petilli MA,Cattaneo L,Marelli M

    更新日期：2019-12-27 00:00:00

  • Quantifying the barrier lowering of ZnO Schottky nanodevices under UV light.

    abstract：:In this study we measured the degrees to which the Schottky barrier heights (SBHs) are lowered in ZnO nanowire (NW) devices under illumination with UV light. We measured the I-V characteristics of ZnO nanowire devices to confirm that ZnO is an n-type semiconductor and that the on/off ratio is approximately 10(4). From...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep15123

    authors： Lu MY,Lu MP,You SJ,Chen CW,Wang YJ

    更新日期：2015-10-12 00:00:00

  • Automatic Identification of Systolic Time Intervals in Seismocardiogram.

    abstract：:Continuous and non-invasive monitoring of hemodynamic parameters through unobtrusive wearable sensors can potentially aid in early detection of cardiac abnormalities, and provides a viable solution for long-term follow-up of patients with chronic cardiovascular diseases without disrupting the daily life activities. El...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep37524

    authors： Shafiq G,Tatinati S,Ang WT,Veluvolu KC

    更新日期：2016-11-22 00:00:00

  • SDE5, a putative RNA export protein, participates in plant innate immunity through a flagellin-dependent signaling pathway in Arabidopsis.

    abstract：:In eukaryotes, RNA silencing, mediated by small interfering RNAs, is an evolutionarily widespread and versatile silencing mechanism that plays an important role in various biological processes. Increasing evidences suggest that various components of RNA silencing pathway are involved in plant defense machinery against...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07918-x

    authors： Uddin MN,Akhter S,Chakraborty R,Baek JH,Cha JY,Park SJ,Kang H,Kim WY,Lee SY,Mackey D,Kim MG

    更新日期：2017-08-29 00:00:00

  • Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan.

    abstract：:Assessing the possible biological effects of exposure to low doses of ionizing radiation (IR) is one of the prime challenges in radiation protection, especially in medical imaging. Today, radiobiological data on cone beam CT (CBCT) related biological effects are scarce. In children and adults, the induction of DNA dou...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-58746-5

    authors： Belmans N,Gilles L,Vermeesen R,Virag P,Hedesiu M,Salmon B,Baatout S,Lucas S,Lambrichts I,Jacobs R,Moreels M,DIMITRA Research Group.

    更新日期：2020-02-07 00:00:00

  • RNA-Seq analysis and comparison of corneal epithelium in keratoconus and myopia patients.

    abstract：:Keratoconus is a common degenerative corneal disease that can lead to significant visual morbidity, and both genetic and environmental factors have been implicated in its pathogenesis. We compared the transcriptome of keratoconus and control epithelium using RNA-Seq. Epithelial tissues were obtained prior to surgery f...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18480-x

    authors： You J,Corley SM,Wen L,Hodge C,Höllhumer R,Madigan MC,Wilkins MR,Sutton G

    更新日期：2018-01-10 00:00:00

  • Contribution to the ecology of the Italian hare (Lepus corsicanus).

    abstract：:The Italian hare (Lepus corsicanus) is endemic to Central-Southern Italy and Sicily, classified as vulnerable due to habitat alterations, low density and fragmented populations and ecological competition with the sympatric European hare (Lepus europaeus). Despite this status, only few and local studies have explored i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-70013-1

    authors： Buglione M,Petrelli S,de Filippo G,Troiano C,Rivieccio E,Notomista T,Maselli V,di Martino L,Carafa M,Gregorio R,Latini R,Fortebraccio M,Romeo G,Biliotti C,Fulgione D

    更新日期：2020-08-04 00:00:00

  • In Vivo Visualization of Stromal Macrophages via label-free FLIM-based metabolite imaging.

    abstract：:Macrophage infiltration and recruitment in breast tumors has been correlated with poor prognosis in breast cancer patients and has been linked to tumor cell dissemination. Much of our understanding comes from animal models in which macrophages are labeled by expression of an extrinsic fluorophore. However, conventiona...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep25086

    authors： Szulczewski JM,Inman DR,Entenberg D,Ponik SM,Aguirre-Ghiso J,Castracane J,Condeelis J,Eliceiri KW,Keely PJ

    更新日期：2016-05-25 00:00:00

  • Plasmid-based and -free methods using CRISPR/Cas9 system for replacement of targeted genes in Colletotrichum sansevieriae.

    abstract：:The CRISPR-Cas9 system has a potential for wide application in organisms that particularly present low homologous integration rates. In this study, we developed three different methods using this system to replace a gene through homology-directed repair in the plant pathogenic fungus Colletotrichum sansevieriae, which...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-55302-8

    authors： Nakamura M,Okamura Y,Iwai H

    更新日期：2019-12-12 00:00:00

  • Isolation and characterization of a novel wheat cysteine-rich receptor-like kinase gene induced by Rhizoctonia cerealis.

    abstract：:Cysteine-rich receptor kinases (CRKs) belong to the receptor-like kinase family. Little is known about CRK genes in wheat. We isolated a wheat CRK gene TaCRK1 from Rhizoctonia cerealis-resistant wheat CI12633 based on a differentially expressed sequence identified by RNA-Sequencing (RNA-Seq) analysis. TaCRK1 was more ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03021

    authors： Yang K,Rong W,Qi L,Li J,Wei X,Zhang Z

    更新日期：2013-10-23 00:00:00

  • Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation.

    abstract：:The physiologic response to tobacco smoke can be measured by gene-expression profiling of the airway epithelium. Temporal resolution of kinetics of gene-expression alterations upon smoking-cessation might delineate distinct biological processes that are activated during recovery from tobacco smoke exposure. Using whol...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43295-3

    authors： Hijazi K,Malyszko B,Steiling K,Xiao X,Liu G,Alekseyev YO,Dumas YM,Hertsgaard L,Jensen J,Hatsukami D,Brooks DR,O'Connor G,Beane J,Lenburg ME,Spira A

    更新日期：2019-05-06 00:00:00