Abstract：
:Macrophage infiltration and recruitment in breast tumors has been correlated with poor prognosis in breast cancer patients and has been linked to tumor cell dissemination. Much of our understanding comes from animal models in which macrophages are labeled by expression of an extrinsic fluorophore. However, conventional extrinsic fluorescence labeling approaches are not readily applied to human tissue and clinical use. We report a novel strategy that exploits endogenous fluorescence from the metabolic co-factors NADH and FAD with quantitation from Fluorescence Lifetime Imaging Microscopy (FLIM) as a means to non-invasively identify tumor-associated macrophages in the intact mammary tumor microenvironment. Macrophages were FAD(HI) and demonstrated a glycolytic-like NADH-FLIM signature that was readily separated from the intrinsic fluorescence signature of tumor cells. This non-invasive quantitative technique provides a unique ability to discern specific cell types based upon their metabolic signatures without the use of exogenous fluorescent labels. Not only does this provide high resolution temporal and spatial views of macrophages in live animal breast cancer models, this approach can be extended to other animal disease models where macrophages are implicated and has potential for clinical applications.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Szulczewski JM,Inman DR,Entenberg D,Ponik SM,Aguirre-Ghiso J,Castracane J,Condeelis J,Eliceiri KW,Keely PJdoi
10.1038/srep25086subject
Has Abstractpub_date
2016-05-25 00:00:00pages
25086issn
2045-2322pii
srep25086journal_volume
6pub_type
杂志文章abstract：:The health risks associated with spaceflight-induced ocular structural and functional damage has become a recent concern for NASA. The goal of the present study was to characterize the effects of spaceflight and reentry to 1 g on the structure and integrity of the retina and blood-retinal barrier (BRB) in the eye. To ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44696-0
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abstract：:Exchange bias (EB) of magnetic nanoparticles (MNPs) in the nanoscale regime has been extensively studied by researchers, which have opened up a novel approach in tuning the magnetic anisotropy properties of magnetic nanoparticles (MNPs) in prospective application of biomedical research such as magnetic hyperthermia. I...
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journal_title：Scientific reports
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
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