当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > RNA-Seq analysis and comparison of corneal epithelium in keratoconus and myopia patients.

RNA-Seq analysis and comparison of corneal epithelium in keratoconus and myopia patients.

Abstract：

:Keratoconus is a common degenerative corneal disease that can lead to significant visual morbidity, and both genetic and environmental factors have been implicated in its pathogenesis. We compared the transcriptome of keratoconus and control epithelium using RNA-Seq. Epithelial tissues were obtained prior to surgery from keratoconus and myopia control patients, undergoing collagen cross-linking and photorefractive keratectomy, respectively. We identified major differences in keratoconus linked to cell-cell communication, cell signalling and cellular metabolism. The genes associated with the Hedgehog, Wnt and Notch1 signaling pathways were down-regulated in keratoconus. We also identified plasmolipin and Notch1 as being significantly reduced in keratoconus for both gene and protein expression (p < 0.05). Plasmolipin is a novel protein identified in human corneal epithelium, and has been demonstrated to have a key role in epithelial cell differentiation in other tissues. This study shows altered gene and protein expression of these three proteins in keratoconus, and further studies are clearly warranted to confirm the functional role of these proteins in the pathogenesis of keratoconus.

journal_name

Sci Rep

journal_title

Scientific reports

authors

You J,Corley SM,Wen L,Hodge C,Höllhumer R,Madigan MC,Wilkins MR,Sutton G

doi

10.1038/s41598-017-18480-x

subject

Has Abstract

pub_date

2018-01-10 00:00:00

pages

389

issue

1

issn

2045-2322

pii

10.1038/s41598-017-18480-x

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Cascaded gain-switching in the mid-infrared region.

    abstract：:In this report, we demonstrate mid-infrared dual-waveband (i.e., ~3 μm and ~2 μm) pulses from a cascaded gain-switched Ho3+-doped ZBLAN fiber laser by the use of hybrid pumping of 1150 nm CW and pulse LDs for the first time. Stable ~3 μm gain-switched pulses with the maximum output power 262.14 mW and shortest pulse d...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17305-1

    authors： Luo H,Li J,Zhu C,Lai X,Hai Y,Liu Y

    更新日期：2017-12-04 00:00:00

  • The FGFR4-388arg Variant Promotes Lung Cancer Progression by N-Cadherin Induction.

    abstract：:The FGFR4-388Arg variant has been related to poor prognosis in several types of cancer, including lung cancer. The mechanism underlying this association has not been addressed in detail in patients with this pathology. Here, we report that this FGFR4 variant induces MAPK and STAT3 activation and causes pro-oncogenic e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-20570-3

    authors： Quintanal-Villalonga Á,Ojeda-Márquez L,Marrugal Á,Yagüe P,Ponce-Aix S,Salinas A,Carnero A,Ferrer I,Molina-Pinelo S,Paz-Ares L

    更新日期：2018-02-05 00:00:00

  • Hydrogen Bond Variations of Influenza A Viruses During Adaptation in Human.

    abstract：:Many host specific mutations have been detected in influenza A viruses (IAVs). However, their effects on hydrogen bond (H-bond) variations have rarely been investigated. In this study, 60 host specific sites were identified in the internal proteins of avian and human IAVs, 27 of which contained mutations with effects ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14533-3

    authors： Luo J,Deng L,Ding X,Quan L,Wu A,Jiang T

    更新日期：2017-10-30 00:00:00

  • Elemental Zn and its Binding Protein Zinc-α2-Glycoprotein are Elevated in HPV-Positive Oropharyngeal Squamous Cell Carcinoma.

    abstract：:Human papillomavirus (HPV)-positive head and neck squamous cell carcinoma (HNSCC) is biologically distinct from HPV-negative HNSCC. Outside of HPV-status, few tumor-intrinsic variables have been identified that correlate to improved survival. As part of exploratory analysis into the trace elemental composition of orop...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-53268-1

    authors： Poropatich K,Paunesku T,Zander A,Wray B,Schipma M,Dalal P,Agulnik M,Chen S,Lai B,Antipova O,Maxey E,Brown K,Wanzer MB,Gursel D,Fan H,Rademaker A,Woloschak GE,Mittal BB

    更新日期：2019-11-18 00:00:00

  • The coupling of Phanerozoic continental weathering and marine phosphorus cycle.

    abstract：:Organic matter production and decomposition primarily modulate the atmospheric O2 and CO2 levels. The long term marine primary productivity is controlled by the terrestrial input of phosphorus (P), while the marine P cycle would also affect organic matter production. In the past 540 million years, the evolution of ter...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-62816-z

    authors： Wang R,Lang X,Ding W,Liu Y,Huang T,Tang W,Shen B

    更新日期：2020-04-02 00:00:00

  • Bidirectional modulation of hippocampal synaptic plasticity by Dopaminergic D4-receptors in the CA1 area of hippocampus.

    abstract：:Long-term potentiation (LTP) is the persistent increase in the strength of the synapses. However, the neural networks would become saturated if there is only synaptic strenghthening. Synaptic weakening could be facilitated by active processes like long-term depression (LTD). Molecular mechanisms that facilitate the we...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-15917-1

    authors： Navakkode S,Chew KCM,Tay SJN,Lin Q,Behnisch T,Soong TW

    更新日期：2017-11-14 00:00:00

  • Temporal-specific complexity of spiking patterns in spontaneous activity induced by a dual complex network structure.

    abstract：:Temporal fluctuation of neural activity in the brain has an important function in optimal information processing. Spontaneous activity is a source of such fluctuation. The distribution of excitatory postsynaptic potentials (EPSPs) between cortical pyramidal neurons can follow a log-normal distribution. Recent studies ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-49286-8

    authors： Nobukawa S,Nishimura H,Yamanishi T

    更新日期：2019-09-04 00:00:00

  • Effect of congenital adrenal hyperplasia treated by glucocorticoids on plasma metabolome: a machine-learning-based analysis.

    abstract：BACKGROUND:Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency leads to impaired cortisol biosynthesis. Treatment includes glucocorticoid supplementation. We studied the specific metabolomics signatures in CAH patients using two different algorithms. METHODS:In a case-control study of CAH patients ma...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-65897-y

    authors： Nguyen LS,Prifti E,Ichou F,Leban M,Funck-Brentano C,Touraine P,Salem JE,Bachelot A

    更新日期：2020-06-01 00:00:00

  • Chronic Sleep Disruption Alters Gut Microbiota, Induces Systemic and Adipose Tissue Inflammation and Insulin Resistance in Mice.

    abstract：:Chronic sleep fragmentation (SF) commonly occurs in human populations, and although it does not involve circadian shifts or sleep deprivation, it markedly alters feeding behaviors ultimately promoting obesity and insulin resistance. These symptoms are known to be related to the host gut microbiota. Mice were exposed t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35405

    authors： Poroyko VA,Carreras A,Khalyfa A,Khalyfa AA,Leone V,Peris E,Almendros I,Gileles-Hillel A,Qiao Z,Hubert N,Farré R,Chang EB,Gozal D

    更新日期：2016-10-14 00:00:00

  • Axial plane optical microscopy.

    abstract：:We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More im...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07253

    authors： Li T,Ota S,Kim J,Wong ZJ,Wang Y,Yin X,Zhang X

    更新日期：2014-12-01 00:00:00

  • UROPA: a tool for Universal RObust Peak Annotation.

    abstract：:The annotation of genomic ranges of interest represents a recurring task for bioinformatics analyses. These ranges can originate from various sources, including peaks called for transcription factor binding sites (TFBS) or histone modification ChIP-seq experiments, chromatin structure and accessibility experiments (su...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02464-y

    authors： Kondili M,Fust A,Preussner J,Kuenne C,Braun T,Looso M

    更新日期：2017-06-01 00:00:00

  • Wave manipulation with magnetically tunable metasurfaces.

    abstract：:Tunable metasurfaces have emerged as an efficient approach to manipulate the wave propagation. Different from previous work concentrating on electrically tunable mechanisms, here we demonstrate a magnetically tunable metasurface composed of ferrite rods and metallic foils. By tuning the thickness of ferrite rods, meta...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-05625-1

    authors： Yang H,Yu T,Wang Q,Lei M

    更新日期：2017-07-14 00:00:00

  • Interface Adhesion and Structural Characterization of Rolled-up GaAs/In0.2Ga0.8As Multilayer Tubes by Coherent Phonon Spectroscopy.

    abstract：:We present a detailed experimental and theoretical study of the acoustic phonon modes in rolled-up multilayers with thickness of the layers in the nanometre and diameters in the micrometre range. We compare our results to planar, unrolled multilayers grown by molecular beam epitaxy. For the planar multilayers the expe...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-05739-6

    authors： Brick D,Engemaier V,Guo Y,Grossmann M,Li G,Grimm D,Schmidt OG,Schubert M,Gusev VE,Hettich M,Dekorsy T

    更新日期：2017-07-14 00:00:00

  • Exploring the effects of anodal and cathodal high definition transcranial direct current stimulation targeting the dorsal anterior cingulate cortex.

    abstract：:The dorsal anterior cingulate cortex (dACC) has been identified as a core region affected by many disorders, representing a promising target for neuromodulation. High Definition-transcranial Direct Current Stimulation (HD-tDCS) is a non-invasive neuromodulation technique that has already shown promising outcomes and h...

    journal_title：Scientific reports

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/s41598-018-22730-x

    authors： To WT,Eroh J,Hart J Jr,Vanneste S

    更新日期：2018-03-13 00:00:00

  • Multimodal Fluorescent Polymer Sensor for Highly Sensitive Detection of Nitroaromatics.

    abstract：:Detection of nitroaromatic explosives with high sensitivity and selectivity is extremely important for civilian and military safety. Here, we report the synthesis and multimodal sensing applications of an emissive alanine based dansyl tagged copolymer P(MMA-co-Dansyl-Ala-HEMA) (DCP), synthesized by RAFT copolymerizati...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43836-w

    authors： Kumar V,Maiti B,Chini MK,De P,Satapathi S

    更新日期：2019-05-13 00:00:00

  • OsKinesin-13A is an active microtubule depolymerase involved in glume length regulation via affecting cell elongation.

    abstract：:Grain size is an important trait influencing both the yield and quality of rice and its major determinant is glume size. However, how glume size is regulated remains largely unknown. Here, we report the characterization of OsKinesin-13A, which regulates cell elongation and glume length in rice. The mutant of OsKinesin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep09457

    authors： Deng ZY,Liu LT,Li T,Yan S,Kuang BJ,Huang SJ,Yan CJ,Wang T

    更新日期：2015-03-25 00:00:00

  • Colorectal cancer diagnostic model utilizing metagenomic and metabolomic data of stool microbial extracellular vesicles.

    abstract：:Colorectal cancer (CRC) is the most common type cancers in the world. CRC occurs sporadically in the majority of cases, indicating the predominant cause of the disease are environmental factors. Diet-induced changes in gut-microbiome are recently supposed to contribute on epidemics of CRC. This study was aimed to inve...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-59529-8

    authors： Kim DJ,Yang J,Seo H,Lee WH,Ho Lee D,Kym S,Park YS,Kim JG,Jang IJ,Kim YK,Cho JY

    更新日期：2020-02-18 00:00:00

  • Different phenotypic plastic responses to predators observed among aphid lineages specialized on different host plants.

    abstract：:The role of intraspecific variation in the magnitude and direction of plastic responses in ecology and evolution is increasingly recognized. However, the factors underlying intraspecific variation in plastic responses remain largely unexplored, particularly for the hypothesis that the herbivores' phenotypic response t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45220-0

    authors： Sentis A,Bertram R,Dardenne N,Ramon-Portugal F,Louit I,Le Trionnaire G,Simon JC,Magro A,Pujol B,Hemptinne JL,Danchin E

    更新日期：2019-06-21 00:00:00

  • A Novel Role For Nanog As An Early Cancer Risk Marker In Patients With Laryngeal Precancerous Lesions.

    abstract：:NANOG is a master regulator of embryonic stem cell pluripotency, found to be frequently aberrantly expressed in a variety of cancers, including laryngeal carcinomas. This study investigates for the first time the role of NANOG expression in early stages of laryngeal tumourigenesis and its potential utility as cancer r...

    journal_title：Scientific reports

    pub_type： 杂志文章,多中心研究

    doi：10.1038/s41598-017-11709-9

    authors： Rodrigo JP,Villaronga MÁ,Menéndez ST,Hermida-Prado F,Quer M,Vilaseca I,Allonca E,Pedregal Mallo D,Astudillo A,García-Pedrero JM

    更新日期：2017-09-11 00:00:00

  • Intravitreal aflibercept for active polypoidal choroidal vasculopathy without active polyps.

    abstract：:The purpose of this study was to evaluate the efficacy of intravitreal aflibercept for active polypoidal choroidal vasculopathy (PCV) without active polyps and to identify prognostic factors. We enrolled 40 eyes from 40 patients who manifested PCV with exudation but without active polyps after prior treatment with pho...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37523-5

    authors： Lee SE,Jang JW,Kang SW,Park KH,Lee DW,Kim JH,Bae K

    更新日期：2019-02-06 00:00:00

  • Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants.

    abstract：:Nitric oxide synthase like enzyme (NOS-like enzyme), which produces nitric oxide, participates in many biological processes. However it remains unidentified and highly controversial that plants do possess a NOS-like enzyme. In this paper, a novel arginine analogue NP1 was designed and developed for the direct identifi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32630

    authors： Chang K,Guo T,Li P,Liu Y,Xu Y,Fang Y,Qian X

    更新日期：2016-09-02 00:00:00

  • Amyloid beta in nasal secretions may be a potential biomarker of Alzheimer's disease.

    abstract：:We investigated the level of amyloid beta (Aβ) in nasal secretions of patients with Alzheimer's disease dementia (ADD) using interdigitated microelectrode (IME) biosensors and determined the predictive value of Aβ in nasal secretions for ADD diagnosis. Nasal secretions were obtained from 35 patients with ADD, 18 with ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41429-1

    authors： Kim YH,Lee SM,Cho S,Kang JH,Minn YK,Park H,Choi SH

    更新日期：2019-03-21 00:00:00

  • Stroboscopic vision and sustained attention during coincidence-anticipation.

    abstract：:We compared coincidence-anticipation performance in normal vision and stroboscopic vision as a function of time-on-task. Participants estimated the arrival time of a real object that moved with constant acceleration (-0.7, 0, +0.7 m/s2) in a pseudo-randomised order across 4 blocks of 30 trials in both vision condition...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18092-5

    authors： Ballester R,Huertas F,Uji M,Bennett SJ

    更新日期：2017-12-20 00:00:00

  • Genomic-enabled prediction models using multi-environment trials to estimate the effect of genotype × environment interaction on prediction accuracy in chickpea.

    abstract：:Genomic selection (GS) by selecting lines prior to field phenotyping using genotyping data has the potential to enhance the rate of genetic gains. Genotype × environment (G × E) interaction inclusion in GS models can improve prediction accuracy hence aid in selection of lines across target environments. Phenotypic dat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-30027-2

    authors： Roorkiwal M,Jarquin D,Singh MK,Gaur PM,Bharadwaj C,Rathore A,Howard R,Srinivasan S,Jain A,Garg V,Kale S,Chitikineni A,Tripathi S,Jones E,Robbins KR,Crossa J,Varshney RK

    更新日期：2018-08-03 00:00:00

  • Horizontally-acquired genetic elements in the mitochondrial genome of a centrohelid Marophrys sp. SRT127.

    abstract：:Mitochondrial genomes exhibit diverse features among eukaryotes in the aspect of gene content, genome structure, and the mobile genetic elements such as introns and plasmids. Although the number of published mitochondrial genomes is increasing at tremendous speed, those of several lineages remain unexplored. Here, we ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41238-6

    authors： Nishimura Y,Shiratori T,Ishida KI,Hashimoto T,Ohkuma M,Inagaki Y

    更新日期：2019-03-19 00:00:00

  • Meta-signature LncRNAs serve as novel biomarkers for colorectal cancer: integrated bioinformatics analysis, experimental validation and diagnostic evaluation.

    abstract：:The aim of this study is to explore the differentially expressed lncRNAs, which may have potential biological function and diagnostic value in colorectal cancer (CRC). Through integrated data mining, we finally identified nine differentially expressed lncRNAs and their potential mRNA targets. After a series of bioinfo...

    journal_title：Scientific reports

    pub_type： 临床试验,杂志文章

    doi：10.1038/srep46572

    authors： Dai M,Chen X,Mo S,Li J,Huang Z,Huang S,Xu J,He B,Zou Y,Chen J,Dai S

    更新日期：2017-04-13 00:00:00

  • A machine learning model with human cognitive biases capable of learning from small and biased datasets.

    abstract：:Human learners can generalize a new concept from a small number of samples. In contrast, conventional machine learning methods require large amounts of data to address the same types of problems. Humans have cognitive biases that promote fast learning. Here, we developed a method to reduce the gap between human beings...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-25679-z

    authors： Taniguchi H,Sato H,Shirakawa T

    更新日期：2018-05-09 00:00:00

  • Liver-specific Repin1 deficiency impairs transient hepatic steatosis in liver regeneration.

    abstract：:Transient hepatic steatosis upon liver resection supposes functional relationships between lipid metabolism and liver regeneration. Repin1 has been suggested as candidate gene for obesity and dyslipidemia by regulating key genes of lipid metabolism and lipid storage. Herein, we characterized the regenerative potential...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-35325-3

    authors： Abshagen K,Degenhardt B,Liebig M,Wendt A,Genz B,Schaeper U,Stumvoll M,Hofmann U,Frank M,Vollmar B,Klöting N

    更新日期：2018-11-15 00:00:00

  • Deficits in memory-guided limb movements impair obstacle avoidance locomotion in Alzheimer's disease mouse model.

    abstract：:Memory function deficits induced by Alzheimer's disease (AD) are believed to be one of the causes of an increased risk of tripping in patients. Working memory contributes to accurate stepping over obstacles during locomotion, and AD-induced deficits of this memory function may lead to an increased risk of contact with...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07220

    authors： Setogawa S,Yamaura H,Arasaki T,Endo S,Yanagihara D

    更新日期：2014-11-27 00:00:00

  • Classification of skin phenotypes caused by diabetes mellitus using complex scattering parameters in the millimeter-wave frequency range.

    abstract：:The pathological skin phenotype caused by hyperglycemia is an important indicator for the progress of diabetes mellitus. An early detection of diabetes assures an early intervention to regulate the carbohydrate metabolism. In this publication a non-invasive detection principle based on the measurement of complex scatt...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-06034-0

    authors： Dornuf F,Martín-Mateos P,Duarte B,Hils B,Bonilla-Manrique OE,Larcher F,Acedo P,Krozer V

    更新日期：2017-07-19 00:00:00