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Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants.

Abstract：

:Nitric oxide synthase like enzyme (NOS-like enzyme), which produces nitric oxide, participates in many biological processes. However it remains unidentified and highly controversial that plants do possess a NOS-like enzyme. In this paper, a novel arginine analogue NP1 was designed and developed for the direct identification and real time tracking of NOS-like enzymes in plant by fluorescence sensing. It could bind NOS-like enzyme efficiently and enter the cell successfully. In vivo fluorescence response results directly proved that NOS-like enzymes did exist in tobacco leaf and would be stimulated by pathogen infection, which also provided a useful chemical tool for the study of the function of NOS-like enzyme in plants.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Chang K,Guo T,Li P,Liu Y,Xu Y,Fang Y,Qian X

doi

10.1038/srep32630

subject

Has Abstract

pub_date

2016-09-02 00:00:00

pages

32630

issn

2045-2322

pii

srep32630

journal_volume

6

pub_type

杂志文章

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