Colorectal cancer diagnostic model utilizing metagenomic and metabolomic data of stool microbial extracellular vesicles.

Abstract:

:Colorectal cancer (CRC) is the most common type cancers in the world. CRC occurs sporadically in the majority of cases, indicating the predominant cause of the disease are environmental factors. Diet-induced changes in gut-microbiome are recently supposed to contribute on epidemics of CRC. This study was aimed to investigate the association of metagenomics and metabolomics in gut extracellular vesicles (EVs) of CRC and healthy subjects. A total of 40 healthy volunteers and 32 patients with CRC were enrolled in this study. Metagenomic profiling by sequencing 16 S rDNA was performed for assessing microbial codiversity. We explored the small molecule metabolites using gas chromatography-time-of-flight mass spectrometry. In total, stool EVs were prepared from 40 healthy volunteers and 32 patients with CRC. Metagenomic profiling demonstrated that bacterial phyla, particularly of Firmicutes and Proteobacteria, were significantly altered in patients with colorectal cancer. Through metabolomics profiling, we determined seven amino acids, four carboxylic acids, and four fatty acids; including short-chain to long chain fatty acids that altered in the disease group. Binary logistic regression was further tested to evaluate the diagnostic performance. In summary, the present findings suggest that gut flora dysbiosis may result in alternation of amino acid metabolism, which may be correlated with the pathogenesis of CRC.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Kim DJ,Yang J,Seo H,Lee WH,Ho Lee D,Kym S,Park YS,Kim JG,Jang IJ,Kim YK,Cho JY

doi

10.1038/s41598-020-59529-8

subject

Has Abstract

pub_date

2020-02-18 00:00:00

pages

2860

issue

1

issn

2045-2322

pii

10.1038/s41598-020-59529-8

journal_volume

10

pub_type

杂志文章
  • Anticancer effects of epigallocatechin-3-gallate nanoemulsion on lung cancer cells through the activation of AMP-activated protein kinase signaling pathway.

    abstract::Epigallocatechin-3-gallate (EGCG), a green tea-derived polyphenol, exhibits antitumor activities. An EGCG nanoemulsion (nano-EGCG) was prepared to improve the stability and reduce the side effects of EGCG for treatment of human lung cancer cells, and the antitumor effects were studied. The possible molecular mechanism...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62136-2

    authors: Chen BH,Hsieh CH,Tsai SY,Wang CY,Wang CC

    更新日期:2020-03-20 00:00:00

  • Small nuclear RNA-mediated modulation of splicing reveals a therapeutic strategy for a TREM2 mutation and its post-transcriptional regulation.

    abstract::Loss-of-function mutations in TREM2 cause Nasu-Hakola disease (NHD), a rare genetic disease characterized by early-onset dementia with leukoencephalopathy and bone cysts. An NHD-associated mutation, c.482 + 2 T > C, disrupts the splice donor site of intron 3 and causes aberrant skipping of exon 3, resulting in the los...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-25204-2

    authors: Yanaizu M,Sakai K,Tosaki Y,Kino Y,Satoh JI

    更新日期:2018-05-02 00:00:00

  • From the Age of 5 Humans Decide Economically, Whereas Crows Exhibit Individual Preferences.

    abstract::Human societies greatly depend on tools, which spare us considerable time and effort. Humans might have evolved a bias to employ tools, using them even when they are unnecessary. This study aimed to investigate whether adult humans and a distantly related habitually tool-using vertebrate species, the New Caledonian cr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16984-0

    authors: Danel S,Osiurak F,von Bayern AMP

    更新日期:2017-12-06 00:00:00

  • A new primitive Neornithischian dinosaur from the Jurassic of Patagonia with gut contents.

    abstract::We describe a new species of an ornithischian dinosaur, Isaberrysaura mollensis gen. et sp. nov. The specimen, consisting in an almost complete skull and incomplete postcranium was collected from the marine-deltaic deposits of the Los Molles Formation (Toarcian-Bajocian), being the first reported dinosaur for this uni...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep42778

    authors: Salgado L,Canudo JI,Garrido AC,Moreno-Azanza M,Martínez LC,Coria RA,Gasca JM

    更新日期:2017-02-16 00:00:00

  • Synchronous primary esophageal squamous cell carcinoma and gastric adenocarcinoma: analysis of 41 cases treated in a single institution.

    abstract::The present study investigated the treatment and survival outcomes of patients with synchronous primary esophageal squamous cell carcinoma and gastric adenocarcinoma. The medical records of 10,783 patients with primary esophageal squamous cancer treated at our institution between 1995 and 2012 were retrospectively rev...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13335

    authors: Li X,Lin S,Zhang Y,Wang H

    更新日期:2015-08-20 00:00:00

  • Author Correction: Early‑life short‑term environmental enrichment counteracts the effects of stress on anxiety‑like behavior, brain‑derived neurotrophic factor and nuclear translocation of glucocorticoid receptors in the basolateral amygdala.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-74322-3

    authors: Hegde A,Suresh S,Mitra R

    更新日期:2020-10-06 00:00:00

  • Identification of DKK-1 as a novel mediator of statin effects in human endothelial cells.

    abstract::This study shows that DKK-1, a member of the Dickkopf family and a regulator of the Wnt pathways, represents a novel target of statins which, through the inhibition of HMG-CoA reductase and of non-steroidal isoprenoid intermediates, exert extra-beneficial effect in preventing atherosclerosis beyond their effect on the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35119-7

    authors: Pontremoli M,Brioschi M,Baetta R,Ghilardi S,Banfi C

    更新日期:2018-11-12 00:00:00

  • Author Correction: Simulation of visual acuity by personalizable neuro-physiological model of the human eye.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-019-54236-5

    authors: Fülep C,Kovács I,Kránitz K,Erdei G

    更新日期:2019-11-22 00:00:00

  • Defining Phenotypes in Diabetic Nephropathy: a novel approach using a cross-sectional analysis of a single centre cohort.

    abstract::The global increase in Diabetes Mellitus (DM) has led to an increase in DM-Chronic Kidney Disease (DM-CKD). In this cross-sectional observational study we aimed to define phenotypes for patients with DM-CKD that in future may be used to individualise treatment We report 4 DM-CKD phenotypes in 220 patients recruited fr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-18595-1

    authors: Montero RM,Herath A,Qureshi A,Esfandiari E,Pusey CD,Frankel AH,Tam FWK

    更新日期:2018-01-08 00:00:00

  • Exo70 is an independent prognostic factor in colon cancer.

    abstract::Exo70, a key component of the Exocyst complex, plays important roles in human cancer progression beyond exocytosis. However, the expression of Exo70 and its prognostic value for patients with colon cancer has not been well investigated to date. In this study, we observed that the mRNA and protein levels of Exo70 were ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05308-x

    authors: Xiao L,Zheng K,Lv X,Hou J,Xu L,Zhao Y,Song F,Fan Y,Cao H,Zhang W,Hong X,Zhan YY,Hu T

    更新日期:2017-07-11 00:00:00

  • Asynchronous multi-decadal time-scale series of biotic and abiotic responses to precipitation during the last 1300 years.

    abstract::East Asian summer monsoon (EASM)-driven rapid hydroclimatic variation is a crucial factor with major socioeconomic impacts. Nevertheless, decadal- to centennial-scale EASM variability over the last two millennia is still poorly understood. Pollen-based quantitative annual precipitation (PqPann) and annual precipitatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74994-x

    authors: Yi S,Jun CP,Jo KN,Lee H,Kim MS,Lee SD,Cao X,Lim J

    更新日期:2020-10-20 00:00:00

  • The non-Newtonian maxwell nanofluid flow between two parallel rotating disks under the effects of magnetic field.

    abstract::The main feature of the present numerical model is to explore the behavior of Maxwell nanoliquid moving within two horizontal rotating disks. The disks are stretchable and subjected to a magnetic field in axial direction. The time dependent characteristics of thermal conductivity have been considered to scrutinize the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74096-8

    authors: Ahmadian A,Bilal M,Khan MA,Asjad MI

    更新日期:2020-10-13 00:00:00

  • miR-200c-driven Mesenchymal-To-Epithelial Transition is a Therapeutic Target in Uterine Carcinosarcomas.

    abstract::Uterine carcinosarcomas (UCSs) are highly aggressive malignancies associated with poor prognoses and limited treatment options. These tumors are hypothesized to develop from the endometrial adenocarcinoma (EAC) through epithelial-mesenchymal transition (EMT). We test this long-standing hypothesis by depleting miR-200,...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03972-7

    authors: Tseng JH,Bisogna M,Hoang LN,Olvera N,Rodriguez-Aguayo C,Lopez-Berestein G,Sood AK,Levine DA,Jelinic P

    更新日期:2017-06-15 00:00:00

  • Clinical and Immunological Effects of rhIL-2 Therapy in Eastern Chinese Patients with Multidrug-resistant Tuberculosis.

    abstract::It is urgent to find an optimised therapy regimen for the control of MDR-TB globally. This study aimed to evaluate the efficiacy and safety of a combined regimen of rhIL-2 injection and standard chemotherapy within 18-month duration in a randomized controlled trial conducted in 14 centres in eastern China. From Jan. 2...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验

    doi:10.1038/s41598-017-18200-5

    authors: Tan Q,Min R,Dai GQ,Wang YL,Nan L,Yang Z,Xia J,Pan SY,Mao H,Xie WP,Wang H

    更新日期:2017-12-19 00:00:00

  • Modeled changes of cerebellar activity in mutant mice are predictive of their learning impairments.

    abstract::Translating neuronal activity to measurable behavioral changes has been a long-standing goal of systems neuroscience. Recently, we have developed a model of phase-reversal learning of the vestibulo-ocular reflex, a well-established, cerebellar-dependent task. The model, comprising both the cerebellar cortex and vestib...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36131

    authors: Badura A,Clopath C,Schonewille M,De Zeeuw CI

    更新日期:2016-11-02 00:00:00

  • Dike intrusions during rifting episodes obey scaling relationships similar to earthquakes.

    abstract::As continental rifts evolve towards mid-ocean ridges, strain is accommodated by repeated episodes of faulting and magmatism. Discrete rifting episodes have been observed along two subaerial divergent plate boundaries, the Krafla segment of the Northern Volcanic Rift Zone in Iceland and the Manda-Hararo segment of the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03886

    authors: Passarelli L,Rivalta E,Shuler A

    更新日期:2014-01-28 00:00:00

  • Immunologic targeting of CD30 eliminates tumourigenic human pluripotent stem cells, allowing safer clinical application of hiPSC-based cell therapy.

    abstract::Induced pluripotent stem cells (iPSCs) are promising candidate cells for cardiomyogenesis in the failing heart. However, teratoma/tumour formation originating from undifferentiated iPSCs contaminating the graft is a critical concern for clinical application. Here, we hypothesized that brentuximab vedotin, which target...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21923-8

    authors: Sougawa N,Miyagawa S,Fukushima S,Kawamura A,Yokoyama J,Ito E,Harada A,Okimoto K,Mochizuki-Oda N,Saito A,Sawa Y

    更新日期:2018-02-27 00:00:00

  • Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice.

    abstract::Abdominal aortic aneurysm (AAA) is a leading cause of sudden death in aged people. Activation of ubiquitin proteasome system (UPS) plays a critical role in the protein quality control and various diseases. However, the functional role of UPS in AAA formation remains unclear. In this study, we found that the proteasome...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15730

    authors: Ren H,Li F,Tian C,Nie H,Wang L,Li HH,Zheng Y

    更新日期:2015-10-28 00:00:00

  • Between-group competition elicits within-group cooperation in children.

    abstract::Aggressive interactions between groups are frequent in human societies and can bear significant fitness costs and benefits (e.g. death or access to resources). During between-group competitive interactions, more cohesive groups (i.e. groups formed by individuals who cooperate in group defence) should out-perform less ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43277

    authors: Majolo B,Maréchal L

    更新日期:2017-02-24 00:00:00

  • CD8+ T cells specific for the islet autoantigen IGRP are restricted in their T cell receptor chain usage.

    abstract::CD8+ T cells directed against beta cell autoantigens are considered relevant for the pathogenesis of type 1 diabetes. Using single cell T cell receptor sequencing of CD8+ T cells specific for the IGRP265-273 epitope, we examined whether there was expansion of clonotypes and sharing of T cell receptor chains in autorea...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep44661

    authors: Fuchs YF,Eugster A,Dietz S,Sebelefsky C,Kühn D,Wilhelm C,Lindner A,Gavrisan A,Knoop J,Dahl A,Ziegler AG,Bonifacio E

    更新日期:2017-03-16 00:00:00

  • A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.

    abstract::Data-independent acquisition mass spectrometry promises higher performance in terms of quantification and reproducibility compared to data-dependent acquisition mass spectrometry methods. To enable high-accuracy quantification of Staphylococcus aureus proteins, we have developed a global ion library for data-independe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10059-w

    authors: Michalik S,Depke M,Murr A,Gesell Salazar M,Kusebauch U,Sun Z,Meyer TC,Surmann K,Pförtner H,Hildebrandt P,Weiss S,Palma Medina LM,Gutjahr M,Hammer E,Becher D,Pribyl T,Hammerschmidt S,Deutsch EW,Bader SL,Hecker M,Mo

    更新日期:2017-09-08 00:00:00

  • Fractal aggregation kinetics contributions to thermal conductivity of nano-suspensions in unsteady thermal convection.

    abstract::Nano-suspensions (NS) exhibit unusual thermophysical behaviors once interparticle aggregations and the shear flows are imposed, which occur ubiquitously in applications but remain poorly understood, because existing theories have not paid these attentions but focused mainly on stationary NS. Here we report the critica...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep39446

    authors: Sui J,Zhao P,Bin-Mohsin B,Zheng L,Zhang X,Cheng Z,Chen Y,Chen G

    更新日期:2016-12-20 00:00:00

  • A minimal double quantum dot.

    abstract::Double quantum dots (DQDs) are a versatile platform for solid-state physics, quantum computation and nanotechnology. The micro-fabrication techniques commonly used to fabricate DQDs are difficult to extend to the atomic scale. Using an alternative approach, which relies on scanning tunneling microscopy and spectroscop...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10814-z

    authors: Zheng H,Zhang J,Berndt R

    更新日期:2017-09-07 00:00:00

  • Seasonal changes in energy expenditure, body temperature and activity patterns in llamas (Lama glama).

    abstract::Mammals typically keep their body temperature (Tb) within a narrow limit with changing environmental conditions. There are indications that some wild ungulates can exhibit certain forms of energy saving mechanisms when ambient temperatures are low and/or food is scarce. Therefore, the aim of the study was to determine...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07946-7

    authors: Riek A,Brinkmann L,Gauly M,Perica J,Ruf T,Arnold W,Hambly C,Speakman JR,Gerken M

    更新日期:2017-08-08 00:00:00

  • Hydrogen segregation and its roles in structural stability and metallization: silane under pressure.

    abstract::We present results from first-principles calculations on silane (SiH4) under pressure. We find that a three dimensional P-3 structure becomes the most stable phase above 241 GPa. A prominent structural feature, which separates the P-3 structure from previously observed/predicted SiH4 structures, is that a fraction of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13039

    authors: Cui W,Shi J,Liu H,Yao Y,Wang H,Iitaka T,Ma Y

    更新日期:2015-08-12 00:00:00

  • Strong quantum scarring by local impurities.

    abstract::We discover and characterise strong quantum scars, or quantum eigenstates resembling classical periodic orbits, in two-dimensional quantum wells perturbed by local impurities. These scars are not explained by ordinary scar theory, which would require the existence of short, moderately unstable periodic orbits in the p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep37656

    authors: Luukko PJ,Drury B,Klales A,Kaplan L,Heller EJ,Räsänen E

    更新日期:2016-11-28 00:00:00

  • Conductive Cellulose Composites with Low Percolation Threshold for 3D Printed Electronics.

    abstract::We are reporting a 3D printable composite paste having strong thixotropic rheology. The composite has been designed and investigated with highly conductive silver nanowires. The optimized electrical percolation threshold from both simulation and experiment is shown from 0.7 vol. % of silver nanowires which is signific...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03365-w

    authors: Park JS,Kim T,Kim WS

    更新日期:2017-06-12 00:00:00

  • The T cell receptor resides in ordered plasma membrane nanodomains that aggregate upon patching of the receptor.

    abstract::Two related models for T cell signalling initiation suggest either that T cell receptor (TCR) engagement leads to its recruitment to ordered membrane domains, often referred to as lipid rafts, where signalling molecules are enriched or that ordered TCR-containing membrane nanodomains coalesce upon TCR engagement. That...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10082

    authors: Dinic J,Riehl A,Adler J,Parmryd I

    更新日期:2015-05-08 00:00:00

  • Estimating sleep parameters using an accelerometer without sleep diary.

    abstract::Wrist worn raw-data accelerometers are used increasingly in large-scale population research. We examined whether sleep parameters can be estimated from these data in the absence of sleep diaries. Our heuristic algorithm uses the variance in estimated z-axis angle and makes basic assumptions about sleep interruptions. ...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1038/s41598-018-31266-z

    authors: van Hees VT,Sabia S,Jones SE,Wood AR,Anderson KN,Kivimäki M,Frayling TM,Pack AI,Bucan M,Trenell MI,Mazzotti DR,Gehrman PR,Singh-Manoux BA,Weedon MN

    更新日期:2018-08-28 00:00:00

  • Experimental study on the location of an acoustic emission source considering refraction in different media.

    abstract::The existing acoustic emission (AE) source location methods assume that acoustic waves propagate along straight lines, and the source location is determined by average wave velocity. Because of the heterogeneity of materials, location results often fail to meet the accuracy requirement. For this reason, an AE source l...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07371-w

    authors: Zhou ZL,Zhou J,Dong LJ,Cai X,Rui YC,Ke CT

    更新日期:2017-08-07 00:00:00