10th European Zebrafish Meeting 2017, Budapest: Husbandry Workshop Summary.

abstract：:One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) pr...

journal_title：Zebrafish

authors： Clay H,Ramakrishnan L

更新日期：2005-01-01 00:00:00

abstract：:Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DN...

journal_title：Zebrafish

authors： Kuriya K,Higashiyama E,Avşar-Ban E,Tamaru Y,Ogata S,Takebayashi S,Ogata M,Okumura K

更新日期：2015-12-01 00:00:00

abstract：:The Johns Hopkins Clinical Compound Library (JHCCL), a collection of Food and Drug Administration (FDA)-approved small molecules (1400), was screened in silico for identification of novel β2AR blockers and tested for hematopoietic stem cell (HSC) expansion and radioprotection in zebrafish embryos. Docking studies, fol...

journal_title：Zebrafish

authors： Dimri M,Joshi J,Chakrabarti R,Sehgal N,Sureshbabu A,Kumar IP

更新日期：2015-02-01 00:00:00

abstract：:Doradidae has been a target of phylogenetic studies over the last few years, but chromosomal information about the family is still scarce. Therefore, new cytogenetic data are provided herein and they are correlated with phylogenetic proposals to contribute to the knowledge of chromosomal evolution within doradids. Cyt...

journal_title：Zebrafish

authors： Baumgärtner L,Paiz LM,Takagui FH,Lui RL,Moreira-Filho O,Giuliano-Caetano L,Portela-Castro ALB,Margarido VP

更新日期：2018-06-01 00:00:00

abstract：:Electronic databases provide effective and efficient management of zebrafish colony operations, but commercially available options are expensive. In this study we have developed a free zebrafish management repository alternative using free Google applications. Husbandry information is logged into a Google Sheets-based...

journal_title：Zebrafish

authors： LaCoursiere CM,Ullmann JFP,Poduri A

更新日期：2018-12-01 00:00:00

abstract：:Mycobacteriosis is a common bacterial infection in laboratory zebrafish caused by several different species and strains of Mycobacterium, including both rapid and slow growers. One control measure used to prevent mycobacterial spread within and between facilities is surface disinfection of eggs. Recent studies have hi...

journal_title：Zebrafish

authors： Chang CT,Amack JD,Whipps CM

更新日期：2016-07-01 00:00:00

abstract：:This study reports the first production of offspring with vitrified sperm from a live-bearing fish Xiphophorus hellerii. The overall goal of this study was to develop streamlined protocols for integration into a standardized approach for vitrification of aquatic species germplasm. The objectives were to (1) estimate a...

journal_title：Zebrafish

authors： Cuevas-Uribe R,Yang H,Daly J,Savage MG,Walter RB,Tiersch TR

更新日期：2011-12-01 00:00:00

abstract：:Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover ...

journal_title：Zebrafish

authors： Don EK,Formella I,Badrock AP,Hall TE,Morsch M,Hortle E,Hogan A,Chow S,Gwee SS,Stoddart JJ,Nicholson G,Chung R,Cole NJ

更新日期：2017-02-01 00:00:00

abstract：:The zebrafish system has been established as a useful model for the study of carcinogenesis. The cytogenetic characterization of the genome is vital for furthering our understanding of the progression of the disease. Establishing a basic description of the zebrafish chromosomal karyotype and markers for each specific ...

journal_title：Zebrafish

authors： Peterson SM,Freeman JL

更新日期：2009-12-01 00:00:00

abstract：:Embryo surface disinfection is utilized in aquaculture to decrease the risk of pathogen introduction into established colonies. Zebrafish embryos are commonly disinfected with unbuffered sodium hypochlorite at 25-50 ppm for 10 min with or without concurrent treatment with chemicals, including pronase (Pron), sodium th...

journal_title：Zebrafish

authors： Peneyra SM,Lerpiriyapong K,Riedel ER,Lipman NS,Lieggi C

更新日期：2020-10-01 00:00:00

abstract：:Pamela Raymond received her B.S., M.S., and Ph.D. degrees from the University of Michigan. She is currently Professor of Molecular, Cellular, and Developmental Biology in the College of Literature, Science, and the Arts at the University of Michigan. She has held academic appointments at Harvard Medical School and the...

journal_title：Zebrafish

authors： Raymond P

更新日期：2005-01-01 00:00:00

abstract：:Spring viremia of carp virus (SVCV) causes the skin hemorrhagic disease in cyprinid species, but its molecular mechanism of skin immune response remains unclear at the protein level. In the present study, the differential proteomics of the zebrafish (Danio rerio) skin in response to SVCV infection were examined by iso...

journal_title：Zebrafish

authors： Liu R,Hu X,Lü A,Song Y,Lian Z,Sun J,Sung YY

更新日期：2020-04-01 00:00:00

abstract：:Understanding living systems requires an in-depth knowledge of the signaling networks that drive cellular homeostasis, regulate intercellular communication, and contribute to cell fates during development. Several resources exist to provide high-throughput data sets or manually curated interaction information from hum...

journal_title：Zebrafish

authors： Csályi K,Fazekas D,Kadlecsik T,Türei D,Gul L,Horváth B,Módos D,Demeter A,Pápai N,Lenti K,Csermely P,Vellai T,Korcsmáros T,Varga M

更新日期：2016-12-01 00:00:00

abstract：:This project was developed to promote understanding of how mathematics and statistical analysis are used as tools in genetic research. It gives students the opportunity to carry out hypothesis-driven experiments in the classroom: students generate hypotheses about Mendelian and non-Mendelian inheritance patterns, gath...

journal_title：Zebrafish

authors： Lindemann S,Senkler J,Auchter E,Liang JO

更新日期：2011-06-01 00:00:00

abstract：:Several factors contributed to the discovery of Wnt genes. On the one hand, the development of research tools in mammalian genetics such as mouse strains with high incidence of cancer in 1920-1930s led to discovery of the mouse mammary tumor virus in 1949. This virus in turn was used in 1982 to identify int-1 (Wnt1) i...

journal_title：Zebrafish

authors： Korzh V

更新日期：2008-09-01 00:00:00

abstract：:The use of antibiotics for anti-infection and growth promotion has caused the overuse of antibiotics in aquaculture. However, the benefit or risk of the long-term use of antibiotics on fish growth or health has not been fully addressed. In the present study, zebrafish were fed with sulfamethoxazole (SMX) or oxytetracy...

journal_title：Zebrafish

authors： Zhou L,Limbu SM,Qiao F,Du ZY,Zhang M

更新日期：2018-08-01 00:00:00

abstract：:There is now strong evidence that early nutrition plays an important role in shaping later physiology. We assessed here whether soy protein concentrate (SPC) or soybean meal (SBM) in early diet would modify zebrafish responses to these products in later life. We fed zebrafish larvae with SPC-, SBM-, or a control-diet ...

journal_title：Zebrafish

authors： Perera E,Yúfera M

更新日期：2016-02-01 00:00:00

abstract：:We have developed a simple method for the induction of cataracts in adult zebrafish using hydrogen peroxide. Our method uses a corneal puncture followed by intraocular injection of hydrogen peroxide into the anterior chamber. The procedure can be done rapidly, forming a highly opaque cataract almost immediately, and f...

journal_title：Zebrafish

authors： Prior HM,Letwin K,Tuininga A,Nguyen M

更新日期：2018-04-01 00:00:00

abstract：:The saltwater rotifer, Brachionus plicatilis, is widely used in the aquaculture industry as a prey item for first-feeding fishes due to its ease of culture, small size, rapid reproductive rate, and amenability to enrichment with nutrients. Despite the distinct advantages of this approach, rotifers have only been spora...

journal_title：Zebrafish

authors： Lawrence C,Sanders E,Henry E

更新日期：2012-09-01 00:00:00

abstract：:Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to mi...

journal_title：Zebrafish

authors： Bill BR,Petzold AM,Clark KJ,Schimmenti LA,Ekker SC

更新日期：2009-03-01 00:00:00

abstract：:The European Zebrafish Principal Investigator Meeting (EZPM) is an ideal forum for group leaders using this fantastic animal model not only to discuss science but also to strengthen their interactions, to push forward technological advances, and to define guidelines for the use of this fish in research. The city of Li...

journal_title：Zebrafish

authors： Lopes SS,Distel M,Linker C,Fior R,Monteiro R,Bianco IH,Portugues R,Strähle U,Saúde L

更新日期：2016-12-01 00:00:00

abstract：:Microcystin-leucine arginine (MC-LR) is a natural toxin produced by cyanobacterial blooms that causes severe liver damage in fish. It is crucial to investigate if housekeeping genes are affected by MC-LR in zebrafish, to permit the adequate evaluation of gene expression by RT-qPCR. We evaluated the gene expression sta...

journal_title：Zebrafish

authors： Sopezki MDS,Monserrat JM,Yunes JS,Zanette J

更新日期：2018-10-01 00:00:00

abstract：:The two LIM kinases (LIMKs) LIMK1 and LIMK2 are members of the PDZ/LIM family. These serine/threonine protein kinases are involved in actin cytoskeleton reorganization through phosphorylation and inactivation of ADF/cofilin. Different subcellular localizations of LIMK1 and LIMK2 suggest different functions. LIMK1 is i...

journal_title：Zebrafish

authors： Vlecken DH,Bagowski CP

更新日期：2009-12-01 00:00:00

abstract：:Dr. Manfred Schartl is Professor in the Department of Physiological Chemistry at the Biocenter of the University of Würzburg, Germany and Adjunct Professor for Experimental Cancer Research at the University of Bergen, Norway. He pursued undergraduate studies in biology and graduate work at the University of Giebetaen,...

journal_title：Zebrafish

authors： Schartl M

更新日期：2004-01-01 00:00:00

abstract：:Many facilities house fish in separate static containers post-procedure, for example, while awaiting genotyping results. This ensures fish can be easily identified, but it does not allow for provision of continuous filtered water or diet. At the Wellcome Trust Sanger Institute, concern over the housing conditions led ...

journal_title：Zebrafish

authors： Goodwin N,Westall L,Karp NA,Hazlehurst D,Kovacs C,Keeble R,Thompson P,Collins R,Bussell J

更新日期：2016-07-01 00:00:00

abstract：:Wild zebrafish exhibit a wide range of behavior. We found abundant wild zebrafish in flowing rivers and still water, in large, tightly-knit groups of hundreds of individuals, as well as in small, loose shoals. In two still-water populations, zebrafish were quite small in body size, common, and in tight groups of up to...

journal_title：Zebrafish

authors： Suriyampola PS,Shelton DS,Shukla R,Roy T,Bhat A,Martins EP

更新日期：2016-02-01 00:00:00

abstract：:Many assays are used in animal model systems to measure specific human disease-related behaviors. The use of both adult and larval zebrafish as a behavioral model is gaining popularity. As this work progresses and potentially translates into new treatments, we must do our best to improve the sensitivity of these assay...

journal_title：Zebrafish

authors： Philpott C,Donack CJ,Cousin MA,Pierret C

更新日期：2012-12-01 00:00:00

abstract：:Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this st...

journal_title：Zebrafish

authors： De Rienzo G,Gutzman JH,Sive H

更新日期：2012-09-01 00:00:00

abstract：:An emergent field of animal personality necessitates a method for repeated high-throughput quantification of behavioral traits across contexts. In this study, we have developed an automated video stimulus approach to sequentially present different contexts relevant to five "personality" traits (exploration, boldness, ...

journal_title：Zebrafish

authors： Fangmeier ML,Noble DWA,O'Dea RE,Usui T,Lagisz M,Hesselson D,Nakagawa S

更新日期：2018-04-01 00:00:00

abstract：:The diversity of Hypancistrus species in the Xingu River is remarkable and the variation in color morphs represents a real challenge to taxonomists to delimit species boundaries. One of the most recognizable Hypancistrus complexes is the worm-lined species, known in the aquarium trade as King Tiger Plec in English, Hy...

journal_title：Zebrafish

authors： Cardoso AL,Carvalho HL,Benathar TC,Serrão SM,Nagamachi CY,Pieczarka JC,Sousa LM,Ready JS,Noronha RC

更新日期：2016-06-01 00:00:00