Abstract:
:Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DNA replication processes of zebrafish (Danio rerio) cells through the direct visualization of replicating DNA in the nucleus and on DNA fiber molecules isolated from the nucleus. We found that zebrafish chromosomal DNA at the nuclear interior was replicated first, followed by replication of DNA at the nuclear periphery, which is reminiscent of the spatiotemporal regulation of mammalian DNA replication. However, the relative duration of interior DNA replication in zebrafish cells was longer compared to mammalian cells, possibly reflecting zebrafish-specific genomic organization. The rate of replication fork progression and ori-to-ori distance measured by the DNA combing technique were ∼ 1.4 kb/min and 100 kb, respectively, which are comparable to those in mammalian cells. To our knowledge, this is a first report that measures replication dynamics in zebrafish cells.
journal_name
Zebrafishjournal_title
Zebrafishauthors
Kuriya K,Higashiyama E,Avşar-Ban E,Tamaru Y,Ogata S,Takebayashi S,Ogata M,Okumura Kdoi
10.1089/zeb.2015.1151subject
Has Abstractpub_date
2015-12-01 00:00:00pages
432-9issue
6eissn
1545-8547issn
1557-8542journal_volume
12pub_type
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