CRISPR Guide RNA Validation In Vitro.

abstract：:Most young researchers leaving the safe haven of postdoctoral life for the unchartered territory of a PI position are confronted with a multitude of new tasks. For those using fish as their favorite model system, this often includes the daunting task of setting up and running a fish facility. New PIs are expected to k...

journal_title：Zebrafish

authors： McNabb A,Scott K,Ochsenstein Ev,Seufert K,Carl M

更新日期：2012-09-01 00:00:00

abstract：:Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover ...

journal_title：Zebrafish

authors： Don EK,Formella I,Badrock AP,Hall TE,Morsch M,Hortle E,Hogan A,Chow S,Gwee SS,Stoddart JJ,Nicholson G,Chung R,Cole NJ

更新日期：2017-02-01 00:00:00

abstract：:Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DN...

journal_title：Zebrafish

authors： Kuriya K,Higashiyama E,Avşar-Ban E,Tamaru Y,Ogata S,Takebayashi S,Ogata M,Okumura K

更新日期：2015-12-01 00:00:00

abstract：:Aggression is a key component of the behavioral repertoire of animals that impacts on their Darwinian fitness. The available genetic tools in zebrafish make this species a promising vertebrate neurogenetic model for the study of neural circuits underlying aggressive behavior. For this purpose, a detailed characterizat...

journal_title：Zebrafish

authors： Oliveira RF,Silva JF,Simões JM

更新日期：2011-06-01 00:00:00

abstract：:We are entering a new era in our ability to modify and edit the genomes of model organisms. Zinc finger nucleases (ZFNs) opened the door to the first custom nuclease-targeted genome engineering in the late 1990s. However, ZFNs remained out of reach for most research labs because of the difficulty of production, high c...

journal_title：Zebrafish

authors： Blackburn PR,Campbell JM,Clark KJ,Ekker SC

更新日期：2013-03-01 00:00:00

abstract：:This study reports the first production of offspring with vitrified sperm from a live-bearing fish Xiphophorus hellerii. The overall goal of this study was to develop streamlined protocols for integration into a standardized approach for vitrification of aquatic species germplasm. The objectives were to (1) estimate a...

journal_title：Zebrafish

authors： Cuevas-Uribe R,Yang H,Daly J,Savage MG,Walter RB,Tiersch TR

更新日期：2011-12-01 00:00:00

abstract：:Comprising a large number of species, the genus Astyanax has been intensively studied by several approaches to elucidate its evolutionary relationships. Such studies have demonstrated that many nominal species are artificial clusters where distinct taxa are grouped under the same denomination. Astyanax aff. fasciatus ...

journal_title：Zebrafish

authors： Kavalco KF,Pazza R,Brandão Kde O,Garcia C,Bertollo LA,de Almeida-Toledo LF

更新日期：2016-08-01 00:00:00

abstract：:The zebrafish mainly uses the sense of light to hunt for food and avoid predators. This reliance on vision necessitates the rapid development of the visual system. Therefore the early larva already exhibits a number of visually- mediated behaviors that can be used for a genetic analysis of vision. The present article ...

journal_title：Zebrafish

authors： Fleisch VC,Neuhauss SC

更新日期：2006-01-01 00:00:00

abstract：:The study of myocardial transmembrane ion currents is fundamental to understand frequent pathologies such as arrhythmias and ischemia. Conventional electrocardiography (ECG) is not able to record ion currents, while the use of intracellular microelectrodes in a beating heart has technical limitations. Myocardial monop...

journal_title：Zebrafish

authors： Miranda M,Egaña JT,Allende ML,Eblen-Zajjur A

更新日期：2019-10-01 00:00:00

abstract：:Zebrafish (Danio rerio) are rapidly gaining popularity in translational neuroscience and behavioral research. Physiological similarity to mammals, ease of genetic manipulations, sensitivity to pharmacological and genetic factors, robust behavior, low cost, and potential for high-throughput screening contribute to the ...

journal_title：Zebrafish

authors： Kalueff AV,Gebhardt M,Stewart AM,Cachat JM,Brimmer M,Chawla JS,Craddock C,Kyzar EJ,Roth A,Landsman S,Gaikwad S,Robinson K,Baatrup E,Tierney K,Shamchuk A,Norton W,Miller N,Nicolson T,Braubach O,Gilman CP,Pittman J

更新日期：2013-03-01 00:00:00

abstract：:Spring viremia of carp virus (SVCV) causes the skin hemorrhagic disease in cyprinid species, but its molecular mechanism of skin immune response remains unclear at the protein level. In the present study, the differential proteomics of the zebrafish (Danio rerio) skin in response to SVCV infection were examined by iso...

journal_title：Zebrafish

authors： Liu R,Hu X,Lü A,Song Y,Lian Z,Sun J,Sung YY

更新日期：2020-04-01 00:00:00

abstract：:Our first Zebrafish Enhancer TRAP lines database (ZETRAP) generated a few years ago was a web-based system informing the scientific community about the developmental, genetic, and genomic aspects of transgenic zebrafish lines expressing the cytosolic version of EGFP. These transgenic lines were obtained in a primary s...

journal_title：Zebrafish

authors： Kondrychyn I,Teh C,Garcia-Lecea M,Guan Y,Kang A,Korzh V

更新日期：2011-12-01 00:00:00

abstract：:The zebrafish, Danio rerio, has emerged as a major model organism for biomedical research, yet little is known about its natural history. We review the literature pertaining to the geographic range, biotic and abiotic habitats, and life cycle of the zebrafish. We also report our own field study to document several asp...

journal_title：Zebrafish

authors： Engeszer RE,Patterson LB,Rao AA,Parichy DM

更新日期：2007-04-01 00:00:00

abstract：:One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) pr...

journal_title：Zebrafish

authors： Clay H,Ramakrishnan L

更新日期：2005-01-01 00:00:00

abstract：:The zebrafish had landed in Singapore as an ornamental fish long before it became a fashionable model of scientific research. By the early 1990s, however, it became a part of the local scientific landscape. During the past decade the number of groups using zebrafish as a research model increased dramatically. In June ...

journal_title：Zebrafish

authors： You MS,Korzh V

更新日期：2005-01-01 00:00:00

abstract：:Zebrafish are increasingly used in neurobiological and behavioral studies. Possible stimuli to manipulate zebrafish behavior are being investigated. The presentation of colors appears to be one of the most used approaches, but there is much debate about the exact color preference. Here we have investigated the color p...

journal_title：Zebrafish

authors： Peeters BW,Moeskops M,Veenvliet AR

更新日期：2016-08-01 00:00:00

abstract：:Astyanax is a genus with a wide distribution ranging from the south United States to north of Patagonia (Argentina). The available cytogenetic data on Astyanax indicate a high karyotypic diversity, with diploid number of 36-52 chromosomes, presence of B chromosomes, heterochromatin polymorphism, and variations with re...

journal_title：Zebrafish

authors： Piscor D,Pozzobon APB,Fernandes CA,Centofante L,Parise-Maltempi PP

更新日期：2019-02-01 00:00:00

abstract：:Understanding living systems requires an in-depth knowledge of the signaling networks that drive cellular homeostasis, regulate intercellular communication, and contribute to cell fates during development. Several resources exist to provide high-throughput data sets or manually curated interaction information from hum...

journal_title：Zebrafish

authors： Csályi K,Fazekas D,Kadlecsik T,Türei D,Gul L,Horváth B,Módos D,Demeter A,Pápai N,Lenti K,Csermely P,Vellai T,Korcsmáros T,Varga M

更新日期：2016-12-01 00:00:00

abstract：:After its introduction for scientific investigation in the 1950s, the cypriniform zebrafish, Danio rerio, has become a valuable model for the study of regenerative processes and mechanisms. Zebrafish exhibit epimorphic regeneration, in which a nondifferentiated cell mass formed after amputation is able to fully regene...

journal_title：Zebrafish

authors： Lebedeva L,Zhumabayeva B,Gebauer T,Kisselev I,Aitasheva Z

更新日期：2020-12-01 00:00:00

abstract：:A review of some exciting new directions for Xiphophorus research in behavioral ecology and integrative animal behavior is presented. The primary objective of this review is to identify areas of research in this teleost genus that, if studied further, could yield insights into the manifold causes and consequences of d...

journal_title：Zebrafish

authors： Earley RL

更新日期：2006-01-01 00:00:00

abstract：:A husbandry workshop on July 3, 2017, at the 10th European Zebrafish Meeting in Budapest, Hungary (July 3-July 7, 2017), focused on the standardization, optimization, and streamlining of fish facility procedures. Standardization can be achieved for example by developing novel software and hardware tools, such as a fis...

journal_title：Zebrafish

authors： Oltová J,Barton C,Certal AC,Argenton F,Varga ZM

更新日期：2018-04-01 00:00:00

abstract：:β-Cells of the islet of Langerhans produce insulin to maintain glucose homeostasis. Self-replication of β-cells is the predominant mode of postnatal β-cell production in mammals, with about 20% of rodent β cells dividing in a 24-hour period. However, replicating β-cells are rare in adults. Induction of self-replicatio...

journal_title：Zebrafish

authors： Li M,Maddison LA,Crees Z,Chen W

更新日期：2013-06-01 00:00:00

abstract：:Wild zebrafish exhibit a wide range of behavior. We found abundant wild zebrafish in flowing rivers and still water, in large, tightly-knit groups of hundreds of individuals, as well as in small, loose shoals. In two still-water populations, zebrafish were quite small in body size, common, and in tight groups of up to...

journal_title：Zebrafish

authors： Suriyampola PS,Shelton DS,Shukla R,Roy T,Bhat A,Martins EP

更新日期：2016-02-01 00:00:00

abstract：:A study was conducted to preliminarily assess the contribution of the intestinal microflora to biotin supply in zebrafish. Biotin and avidin were added to three isonitrogenous and isocaloric purified diets to provide molar avidin: biotin ratios of 0:0 (basal diet), 0:1 (biotin-supplemented diet), and 120:0. Another di...

journal_title：Zebrafish

authors： Yossa R,Sarker PK,Vandenberg GW

更新日期：2011-12-01 00:00:00

abstract：:There is now strong evidence that early nutrition plays an important role in shaping later physiology. We assessed here whether soy protein concentrate (SPC) or soybean meal (SBM) in early diet would modify zebrafish responses to these products in later life. We fed zebrafish larvae with SPC-, SBM-, or a control-diet ...

journal_title：Zebrafish

authors： Perera E,Yúfera M

更新日期：2016-02-01 00:00:00

abstract：:The monotypic Astroblepidae fish family includes 81 species distributed along Central and Northern Andes in South America and Panamá in Central America; most aspects regarding its biology, taxonomy, and chromosomal features remain largely unknown. This study reports the karyotype of two sympatric Astroblepus species f...

journal_title：Zebrafish

authors： Conde-Saldaña CC,Cunha MS,Albornoz-Garzón JG,Barreto CAV,Ibagón N,Villa-Navarro FA,Dergam JA

更新日期：2019-02-01 00:00:00

abstract：:The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacen...

journal_title：Zebrafish

authors： Alves-Silva AP,Dergam JA

更新日期：2015-02-01 00:00:00

abstract：:An emergent field of animal personality necessitates a method for repeated high-throughput quantification of behavioral traits across contexts. In this study, we have developed an automated video stimulus approach to sequentially present different contexts relevant to five "personality" traits (exploration, boldness, ...

journal_title：Zebrafish

authors： Fangmeier ML,Noble DWA,O'Dea RE,Usui T,Lagisz M,Hesselson D,Nakagawa S

更新日期：2018-04-01 00:00:00

abstract：:For many years, zebrafish have been the prototypical model for studies in developmental biology. In recent years, zebrafish has emerged as a powerful model system to study infectious diseases, including viral infections. Experiments conducted with herpes simplex virus type-1 in adult zebrafish or in embryo models are ...

journal_title：Zebrafish

authors： Antoine TE,Jones KS,Dale RM,Shukla D,Tiwari V

更新日期：2014-02-01 00:00:00

abstract：:Managing the welfare of laboratory animals is critical to animal health, vital in the understanding of phenotypes created by treatment or genetic alteration and ensures compliance of regulations. Part of an animal welfare assessment is the requirement to record observations, ensuring all those responsible for the anim...

journal_title：Zebrafish

authors： Goodwin N,Karp NA,Blackledge S,Clark B,Keeble R,Kovacs C,Murray KN,Price M,Thompson P,Bussell J

更新日期：2016-07-01 00:00:00