A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease.

Abstract:

:Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.

journal_name

Zebrafish

journal_title

Zebrafish

authors

Don EK,Formella I,Badrock AP,Hall TE,Morsch M,Hortle E,Hogan A,Chow S,Gwee SS,Stoddart JJ,Nicholson G,Chung R,Cole NJ

doi

10.1089/zeb.2016.1321

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

69-72

issue

1

eissn

1545-8547

issn

1557-8542

journal_volume

14

pub_type

杂志文章
  • Impact of Pronase, Sodium Thiosulfate, and Methylene Blue Combinations on Development and Survival of Sodium Hypochlorite Surface-Disinfected Zebrafish (Danio rerio) Embryos.

    abstract::Embryo surface disinfection is utilized in aquaculture to decrease the risk of pathogen introduction into established colonies. Zebrafish embryos are commonly disinfected with unbuffered sodium hypochlorite at 25-50 ppm for 10 min with or without concurrent treatment with chemicals, including pronase (Pron), sodium th...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2020.1917

    authors: Peneyra SM,Lerpiriyapong K,Riedel ER,Lipman NS,Lieggi C

    更新日期:2020-10-01 00:00:00

  • Investigator profile. An interview with Pamela Raymond, Ph.D. Interview by Vicki Glaser.

    abstract::Pamela Raymond received her B.S., M.S., and Ph.D. degrees from the University of Michigan. She is currently Professor of Molecular, Cellular, and Developmental Biology in the College of Literature, Science, and the Arts at the University of Michigan. She has held academic appointments at Harvard Medical School and the...

    journal_title:Zebrafish

    pub_type: 面试

    doi:10.1089/zeb.2005.2.157

    authors: Raymond P

    更新日期:2005-01-01 00:00:00

  • Investigator profile. An interview with Zhiyuan Gong, Ph.D. Interview by Vicki Glaser.

    abstract::Dr. Zhiyuan Gong is Associate Professor in the Department of Biological Sciences, National University of Singapore. He graduated with a B.Sc. degree in marine biology from Ocean University of China and received his Ph.D. degree in developmental biology from McGill University in Canada. He spent many years at the Unive...

    journal_title:Zebrafish

    pub_type: 面试

    doi:10.1089/zeb.2004.1.185

    authors: Gong Z

    更新日期:2004-01-01 00:00:00

  • Zebrafish in the tropical One-North.

    abstract::The zebrafish had landed in Singapore as an ornamental fish long before it became a fashionable model of scientific research. By the early 1990s, however, it became a part of the local scientific landscape. During the past decade the number of groups using zebrafish as a research model increased dramatically. In June ...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2005.1.327

    authors: You MS,Korzh V

    更新日期:2005-01-01 00:00:00

  • In Vivo Determination of Body Composition in Zebrafish (Danio rerio) by Quantitative Magnetic Resonance.

    abstract::Zebrafish (Danio rerio) as a model research organism continues to expand its relevance and role in multiple research disciplines, with recent work directed toward models of metabolism, nutrition, and energetics. Multiple technologies exist to assess body composition in animal research models at various levels of detai...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2015.1157

    authors: Fowler LA,Dennis LN,Barry RJ,Powell ML,Watts SA,Smith DL Jr

    更新日期:2016-06-01 00:00:00

  • The CRISPR system--keeping zebrafish gene targeting fresh.

    abstract::We are entering a new era in our ability to modify and edit the genomes of model organisms. Zinc finger nucleases (ZFNs) opened the door to the first custom nuclease-targeted genome engineering in the late 1990s. However, ZFNs remained out of reach for most research labs because of the difficulty of production, high c...

    journal_title:Zebrafish

    pub_type: 杂志文章,评审

    doi:10.1089/zeb.2013.9999

    authors: Blackburn PR,Campbell JM,Clark KJ,Ekker SC

    更新日期:2013-03-01 00:00:00

  • Cryptic speciation within the Neotropical cichlid Geophagus brasiliensis (Quoy & Gaimard, 1824) (Teleostei Cichlidae): a new paradigm in karyotypical and molecular evolution.

    abstract::The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacen...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2014.0999

    authors: Alves-Silva AP,Dergam JA

    更新日期:2015-02-01 00:00:00

  • Exploring differential gene expression in zebrafish to teach basic molecular biology skills.

    abstract::In an effort to engage students in original research while teaching them basic molecular biology skills, we have designed a course for upper level undergraduate students and beginning graduate students that employs in situ hybridization in whole-mount zebrafish embryos to explore the concept of differential gene regul...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2008.0574

    authors: Schmoldt A,Forecki J,Hammond DR,Udvadia AJ

    更新日期:2009-06-01 00:00:00

  • Don't be afraid to set up your fish facility.

    abstract::Most young researchers leaving the safe haven of postdoctoral life for the unchartered territory of a PI position are confronted with a multitude of new tasks. For those using fish as their favorite model system, this often includes the daunting task of setting up and running a fish facility. New PIs are expected to k...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2012.0768

    authors: McNabb A,Scott K,Ochsenstein Ev,Seufert K,Carl M

    更新日期:2012-09-01 00:00:00

  • Report of the 4th European Zebrafish Principal Investigator Meeting.

    abstract::The European Zebrafish Principal Investigator Meeting (EZPM) is an ideal forum for group leaders using this fantastic animal model not only to discuss science but also to strengthen their interactions, to push forward technological advances, and to define guidelines for the use of this fish in research. The city of Li...

    journal_title:Zebrafish

    pub_type:

    doi:10.1089/zeb.2016.1364

    authors: Lopes SS,Distel M,Linker C,Fior R,Monteiro R,Bianco IH,Portugues R,Strähle U,Saúde L

    更新日期:2016-12-01 00:00:00

  • Dominance hierarchies in zebrafish (Danio rerio) and their relationship with reproductive success.

    abstract::The zebrafish has considerable potential for use as a model in the study of behavior in social systems, particularly dominance hierarchies, which are widespread in nature and can affect the lifelong success of individuals. There is, however, a paucity of information relating to the characterization of social groups an...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2009.0618

    authors: Paull GC,Filby AL,Giddins HG,Coe TS,Hamilton PB,Tyler CR

    更新日期:2010-03-01 00:00:00

  • Zebrafish Enhancer TRAP transgenic line database ZETRAP 2.0.

    abstract::Our first Zebrafish Enhancer TRAP lines database (ZETRAP) generated a few years ago was a web-based system informing the scientific community about the developmental, genetic, and genomic aspects of transgenic zebrafish lines expressing the cytosolic version of EGFP. These transgenic lines were obtained in a primary s...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2011.0718

    authors: Kondrychyn I,Teh C,Garcia-Lecea M,Guan Y,Kang A,Korzh V

    更新日期:2011-12-01 00:00:00

  • Integrated Cytogenetic and Mitochondrial DNA Analyses Indicate That Two Different Phenotypes of Hypancistrus (L066 and L333) Belong to the Same Species.

    abstract::The diversity of Hypancistrus species in the Xingu River is remarkable and the variation in color morphs represents a real challenge to taxonomists to delimit species boundaries. One of the most recognizable Hypancistrus complexes is the worm-lined species, known in the aquarium trade as King Tiger Plec in English, Hy...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2015.1213

    authors: Cardoso AL,Carvalho HL,Benathar TC,Serrão SM,Nagamachi CY,Pieczarka JC,Sousa LM,Ready JS,Noronha RC

    更新日期:2016-06-01 00:00:00

  • Reducing the noise in behavioral assays: sex and age in adult zebrafish locomotion.

    abstract::Many assays are used in animal model systems to measure specific human disease-related behaviors. The use of both adult and larval zebrafish as a behavioral model is gaining popularity. As this work progresses and potentially translates into new treatments, we must do our best to improve the sensitivity of these assay...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2012.0764

    authors: Philpott C,Donack CJ,Cousin MA,Pierret C

    更新日期:2012-12-01 00:00:00

  • Low-cost aquatic lab animal holding system.

    abstract::We have constructed a low-cost aquatic animal holding system that provides an alternative to expensive, commercially available systems. Our flow-through system is especially useful for programs that are limited in space and funding. The easy assembly and maintenance of the system are advantages for the researchers who...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2006.0501

    authors: Hohn C,Petrie-Hanson L

    更新日期:2007-07-01 00:00:00

  • Zebrafish Embryo Disinfection with Povidone-Iodine: Evaluating an Alternative to Chlorine Bleach.

    abstract::Mycobacteriosis is a common bacterial infection in laboratory zebrafish caused by several different species and strains of Mycobacterium, including both rapid and slow growers. One control measure used to prevent mycobacterial spread within and between facilities is surface disinfection of eggs. Recent studies have hi...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2015.1229

    authors: Chang CT,Amack JD,Whipps CM

    更新日期:2016-07-01 00:00:00

  • Zebrafish (Danio rerio) as a Model for Understanding the Process of Caudal Fin Regeneration.

    abstract::After its introduction for scientific investigation in the 1950s, the cypriniform zebrafish, Danio rerio, has become a valuable model for the study of regenerative processes and mechanisms. Zebrafish exhibit epimorphic regeneration, in which a nondifferentiated cell mass formed after amputation is able to fully regene...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2020.1926

    authors: Lebedeva L,Zhumabayeva B,Gebauer T,Kisselev I,Aitasheva Z

    更新日期:2020-12-01 00:00:00

  • An interview with Manfred Schartl, Ph.D. Interview by Vicki Glaser.

    abstract::Dr. Manfred Schartl is Professor in the Department of Physiological Chemistry at the Biocenter of the University of Würzburg, Germany and Adjunct Professor for Experimental Cancer Research at the University of Bergen, Norway. He pursued undergraduate studies in biology and graduate work at the University of Giebetaen,...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2004.1.77

    authors: Schartl M

    更新日期:2004-01-01 00:00:00

  • A student team in a University of Michigan biomedical engineering design course constructs a microfluidic bioreactor for studies of zebrafish development.

    abstract::The zebrafish is a valuable model for teaching developmental, molecular, and cell biology; aquatic sciences; comparative anatomy; physiology; and genetics. Here we demonstrate that zebrafish provide an excellent model system to teach engineering principles. A seven-member undergraduate team in a biomedical engineering...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2008.0572

    authors: Shen YC,Li D,Al-Shoaibi A,Bersano-Begey T,Chen H,Ali S,Flak B,Perrin C,Winslow M,Shah H,Ramamurthy P,Schmedlen RH,Takayama S,Barald KF

    更新日期:2009-06-01 00:00:00

  • Efficient shRNA-mediated inhibition of gene expression in zebrafish.

    abstract::Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this st...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2012.0770

    authors: De Rienzo G,Gutzman JH,Sive H

    更新日期:2012-09-01 00:00:00

  • Preliminary evidence of the contribution of the intestinal microflora to biotin supply in zebrafish Danio rerio (Hamilton-Buchanan).

    abstract::A study was conducted to preliminarily assess the contribution of the intestinal microflora to biotin supply in zebrafish. Biotin and avidin were added to three isonitrogenous and isocaloric purified diets to provide molar avidin: biotin ratios of 0:0 (basal diet), 0:1 (biotin-supplemented diet), and 120:0. Another di...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2011.0706

    authors: Yossa R,Sarker PK,Vandenberg GW

    更新日期:2011-12-01 00:00:00

  • Direct Visualization of DNA Replication Dynamics in Zebrafish Cells.

    abstract::Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DN...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2015.1151

    authors: Kuriya K,Higashiyama E,Avşar-Ban E,Tamaru Y,Ogata S,Takebayashi S,Ogata M,Okumura K

    更新日期:2015-12-01 00:00:00

  • 10th European Zebrafish Meeting 2017, Budapest: Husbandry Workshop Summary.

    abstract::A husbandry workshop on July 3, 2017, at the 10th European Zebrafish Meeting in Budapest, Hungary (July 3-July 7, 2017), focused on the standardization, optimization, and streamlining of fish facility procedures. Standardization can be achieved for example by developing novel software and hardware tools, such as a fis...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2017.1548

    authors: Oltová J,Barton C,Certal AC,Argenton F,Varga ZM

    更新日期:2018-04-01 00:00:00

  • Color Preference in Danio rerio: Effects of Age and Anxiolytic Treatments.

    abstract::Zebrafish are increasingly used in neurobiological and behavioral studies. Possible stimuli to manipulate zebrafish behavior are being investigated. The presentation of colors appears to be one of the most used approaches, but there is much debate about the exact color preference. Here we have investigated the color p...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2015.1150

    authors: Peeters BW,Moeskops M,Veenvliet AR

    更新日期:2016-08-01 00:00:00

  • Karyotypic Divergence of Two Co-Occurring Species of Andean Climbing Catfishes (Siluriformes: Loricarioidei: Astroblepidae).

    abstract::The monotypic Astroblepidae fish family includes 81 species distributed along Central and Northern Andes in South America and Panamá in Central America; most aspects regarding its biology, taxonomy, and chromosomal features remain largely unknown. This study reports the karyotype of two sympatric Astroblepus species f...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2018.1659

    authors: Conde-Saldaña CC,Cunha MS,Albornoz-Garzón JG,Barreto CAV,Ibagón N,Villa-Navarro FA,Dergam JA

    更新日期:2019-02-01 00:00:00

  • Comparative Developmental Toxicity and Stress Protein Responses of Dimethyl Sulfoxide to Rare Minnow and Zebrafish Embryos/Larvae.

    abstract::Dimethyl sulfoxide (DMSO), a widely used carrier solvent, can be toxic to test organisms and has species-specific sensitivity. In this study, the developmental toxicity and stress protein responses of DMSO to rare minnow (Gobiocypris rarus) and zebrafish (Danio rerio) with two tests were compared in the early life sta...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2016.1287

    authors: Xiong X,Luo S,Wu B,Wang J

    更新日期:2017-02-01 00:00:00

  • Targeted overexpression of CKI-insensitive cyclin-dependent kinase 4 increases functional β-cell number through enhanced self-replication in zebrafish.

    abstract::β-Cells of the islet of Langerhans produce insulin to maintain glucose homeostasis. Self-replication of β-cells is the predominant mode of postnatal β-cell production in mammals, with about 20% of rodent β cells dividing in a 24-hour period. However, replicating β-cells are rare in adults. Induction of self-replicatio...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2012.0816

    authors: Li M,Maddison LA,Crees Z,Chen W

    更新日期:2013-06-01 00:00:00

  • Effects of Parental Aging During Embryo Development and Adult Life: The Case of Nothobranchius furzeri.

    abstract::Studies on parental aging are a very attractive field, although it is poorly understood how parental age affects embryonic development and adult traits of the offspring. In this study, we used the turquoise killifish Nothobranchius furzeri, as is the vertebrate with shortest captive lifespan and an interesting model. ...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2017.1494

    authors: Api M,Biondi P,Olivotto I,Terzibasi E,Cellerino A,Carnevali O

    更新日期:2018-04-01 00:00:00

  • Comparative Cytogenetics Analysis on Five Genera of Thorny Catfish (Siluriformes, Doradidae): Chromosome Review in the Family and Inferences About Chromosomal Evolution Integrated with Phylogenetic Proposals.

    abstract::Doradidae has been a target of phylogenetic studies over the last few years, but chromosomal information about the family is still scarce. Therefore, new cytogenetic data are provided herein and they are correlated with phylogenetic proposals to contribute to the knowledge of chromosomal evolution within doradids. Cyt...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2017.1554

    authors: Baumgärtner L,Paiz LM,Takagui FH,Lui RL,Moreira-Filho O,Giuliano-Caetano L,Portela-Castro ALB,Margarido VP

    更新日期:2018-06-01 00:00:00

  • Using zebrafish to learn statistical analysis and Mendelian genetics.

    abstract::This project was developed to promote understanding of how mathematics and statistical analysis are used as tools in genetic research. It gives students the opportunity to carry out hypothesis-driven experiments in the classroom: students generate hypotheses about Mendelian and non-Mendelian inheritance patterns, gath...

    journal_title:Zebrafish

    pub_type: 杂志文章

    doi:10.1089/zeb.2010.0686

    authors: Lindemann S,Senkler J,Auchter E,Liang JO

    更新日期:2011-06-01 00:00:00