Reducing the noise in behavioral assays: sex and age in adult zebrafish locomotion.

abstract：:The two LIM kinases (LIMKs) LIMK1 and LIMK2 are members of the PDZ/LIM family. These serine/threonine protein kinases are involved in actin cytoskeleton reorganization through phosphorylation and inactivation of ADF/cofilin. Different subcellular localizations of LIMK1 and LIMK2 suggest different functions. LIMK1 is i...

journal_title：Zebrafish

authors： Vlecken DH,Bagowski CP

更新日期：2009-12-01 00:00:00

abstract：:Spring viremia of carp virus (SVCV) causes the skin hemorrhagic disease in cyprinid species, but its molecular mechanism of skin immune response remains unclear at the protein level. In the present study, the differential proteomics of the zebrafish (Danio rerio) skin in response to SVCV infection were examined by iso...

journal_title：Zebrafish

authors： Liu R,Hu X,Lü A,Song Y,Lian Z,Sun J,Sung YY

更新日期：2020-04-01 00:00:00

abstract：:The Johns Hopkins Clinical Compound Library (JHCCL), a collection of Food and Drug Administration (FDA)-approved small molecules (1400), was screened in silico for identification of novel β2AR blockers and tested for hematopoietic stem cell (HSC) expansion and radioprotection in zebrafish embryos. Docking studies, fol...

journal_title：Zebrafish

authors： Dimri M,Joshi J,Chakrabarti R,Sehgal N,Sureshbabu A,Kumar IP

更新日期：2015-02-01 00:00:00

abstract：:Dr. Zhiyuan Gong is Associate Professor in the Department of Biological Sciences, National University of Singapore. He graduated with a B.Sc. degree in marine biology from Ocean University of China and received his Ph.D. degree in developmental biology from McGill University in Canada. He spent many years at the Unive...

journal_title：Zebrafish

authors： Gong Z

更新日期：2004-01-01 00:00:00

abstract：:Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this st...

journal_title：Zebrafish

authors： De Rienzo G,Gutzman JH,Sive H

更新日期：2012-09-01 00:00:00

abstract：:One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) pr...

journal_title：Zebrafish

authors： Clay H,Ramakrishnan L

更新日期：2005-01-01 00:00:00

abstract：:The monotypic Astroblepidae fish family includes 81 species distributed along Central and Northern Andes in South America and Panamá in Central America; most aspects regarding its biology, taxonomy, and chromosomal features remain largely unknown. This study reports the karyotype of two sympatric Astroblepus species f...

journal_title：Zebrafish

authors： Conde-Saldaña CC,Cunha MS,Albornoz-Garzón JG,Barreto CAV,Ibagón N,Villa-Navarro FA,Dergam JA

更新日期：2019-02-01 00:00:00

abstract：:Zebrafish embryos are widely used as a model to monitor the effect of chemicals on their survival and hatching at different time epochs. This experimental design generates longitudinal data in which the observations for a given subject are correlated and they are statistically independent across the subjects. This par...

journal_title：Zebrafish

authors： Aparna C,Sanjeev S,Ajit J,Shantaram K,Jayesh B,Suresh AK

更新日期：2014-08-01 00:00:00

abstract：:Analysis of genes compromising the glomerular filtration barrier in rodent models using transgenic or knockdown approaches is time- and resource-consuming and often leads to unsatisfactory results. Therefore, it would be beneficial to have a selection tool indicating that your gene of interest is in fact associated wi...

journal_title：Zebrafish

authors： Hanke N,King BL,Vaske B,Haller H,Schiffer M

更新日期：2015-10-01 00:00:00

abstract：:Wild zebrafish exhibit a wide range of behavior. We found abundant wild zebrafish in flowing rivers and still water, in large, tightly-knit groups of hundreds of individuals, as well as in small, loose shoals. In two still-water populations, zebrafish were quite small in body size, common, and in tight groups of up to...

journal_title：Zebrafish

authors： Suriyampola PS,Shelton DS,Shukla R,Roy T,Bhat A,Martins EP

更新日期：2016-02-01 00:00:00

abstract：:A husbandry workshop on July 3, 2017, at the 10th European Zebrafish Meeting in Budapest, Hungary (July 3-July 7, 2017), focused on the standardization, optimization, and streamlining of fish facility procedures. Standardization can be achieved for example by developing novel software and hardware tools, such as a fis...

journal_title：Zebrafish

authors： Oltová J,Barton C,Certal AC,Argenton F,Varga ZM

更新日期：2018-04-01 00:00:00

abstract：:There is now strong evidence that early nutrition plays an important role in shaping later physiology. We assessed here whether soy protein concentrate (SPC) or soybean meal (SBM) in early diet would modify zebrafish responses to these products in later life. We fed zebrafish larvae with SPC-, SBM-, or a control-diet ...

journal_title：Zebrafish

authors： Perera E,Yúfera M

更新日期：2016-02-01 00:00:00

abstract：:Our first Zebrafish Enhancer TRAP lines database (ZETRAP) generated a few years ago was a web-based system informing the scientific community about the developmental, genetic, and genomic aspects of transgenic zebrafish lines expressing the cytosolic version of EGFP. These transgenic lines were obtained in a primary s...

journal_title：Zebrafish

authors： Kondrychyn I,Teh C,Garcia-Lecea M,Guan Y,Kang A,Korzh V

更新日期：2011-12-01 00:00:00

abstract：:The saltwater rotifer, Brachionus plicatilis, is widely used in the aquaculture industry as a prey item for first-feeding fishes due to its ease of culture, small size, rapid reproductive rate, and amenability to enrichment with nutrients. Despite the distinct advantages of this approach, rotifers have only been spora...

journal_title：Zebrafish

authors： Lawrence C,Sanders E,Henry E

更新日期：2012-09-01 00:00:00

abstract：:Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DN...

journal_title：Zebrafish

authors： Kuriya K,Higashiyama E,Avşar-Ban E,Tamaru Y,Ogata S,Takebayashi S,Ogata M,Okumura K

更新日期：2015-12-01 00:00:00

abstract：:Zebrafish (Danio rerio) as a model research organism continues to expand its relevance and role in multiple research disciplines, with recent work directed toward models of metabolism, nutrition, and energetics. Multiple technologies exist to assess body composition in animal research models at various levels of detai...

journal_title：Zebrafish

authors： Fowler LA,Dennis LN,Barry RJ,Powell ML,Watts SA,Smith DL Jr

更新日期：2016-06-01 00:00:00

abstract：:Considering the numbers of zebrafish held in the laboratories, it is relevant to develop some tools to monitor the health of the animals, as well as their biotope. Environmental samples can be used to detect aquatic pathogens. Comprehensive health monitoring would thus seek pathogens in three dimensions of the animals...

journal_title：Zebrafish

authors： Mocho JP

更新日期：2016-07-01 00:00:00

abstract：:In an effort to engage students in original research while teaching them basic molecular biology skills, we have designed a course for upper level undergraduate students and beginning graduate students that employs in situ hybridization in whole-mount zebrafish embryos to explore the concept of differential gene regul...

journal_title：Zebrafish

authors： Schmoldt A,Forecki J,Hammond DR,Udvadia AJ

更新日期：2009-06-01 00:00:00

abstract：:Astyanax is a genus with a wide distribution ranging from the south United States to north of Patagonia (Argentina). The available cytogenetic data on Astyanax indicate a high karyotypic diversity, with diploid number of 36-52 chromosomes, presence of B chromosomes, heterochromatin polymorphism, and variations with re...

journal_title：Zebrafish

authors： Piscor D,Pozzobon APB,Fernandes CA,Centofante L,Parise-Maltempi PP

更新日期：2019-02-01 00:00:00

abstract：:The Cannabis sativa plant contains numerous phytocannabinoids and terpenes with known or potential biological activity. For decades, plant breeders have been breeding the Cannabis plant to control for a desired ratio of the major cannabinoids. A high-throughput in vivo model to understand the relationship between the ...

journal_title：Zebrafish

authors： Achenbach JC,Hill J,Hui JPM,Morash MG,Berrue F,Ellis LD

更新日期：2018-08-01 00:00:00

abstract：:We have shown that zebrafish (Danio rerio) are an excellent model for evaluating the link between early life stage exposure to environmental chemicals and disease in adulthood and subsequent unexposed generations. Previously, we used this model to identify transgenerational effects of dioxin (2,3,7,8-tetrachlorodibenz...

journal_title：Zebrafish

authors： Baker BB,Yee JS,Meyer DN,Yang D,Baker TR

更新日期：2016-10-01 00:00:00

abstract：:Although zebrafish is used as a major model species for understanding a number of biological functions and mechanisms involved, there is practically no information on the nutritional requirements of this cyprinid. The rearing practices, especially with regard to feeds and feeding, also vary considerably. To obtain bas...

journal_title：Zebrafish

authors： Kaushik S,Georga I,Koumoundouros G

更新日期：2011-06-01 00:00:00

abstract：:The European Zebrafish Principal Investigator Meeting (EZPM) is an ideal forum for group leaders using this fantastic animal model not only to discuss science but also to strengthen their interactions, to push forward technological advances, and to define guidelines for the use of this fish in research. The city of Li...

journal_title：Zebrafish

authors： Lopes SS,Distel M,Linker C,Fior R,Monteiro R,Bianco IH,Portugues R,Strähle U,Saúde L

更新日期：2016-12-01 00:00:00

abstract：:Electronic databases provide effective and efficient management of zebrafish colony operations, but commercially available options are expensive. In this study we have developed a free zebrafish management repository alternative using free Google applications. Husbandry information is logged into a Google Sheets-based...

journal_title：Zebrafish

authors： LaCoursiere CM,Ullmann JFP,Poduri A

更新日期：2018-12-01 00:00:00

abstract：:Individual differences (IDs) in behavior among nonhuman animals have been documented in a wide range of taxa. Although traditionally considered noise around an average, other potentially adaptive sources of phenotypic variation exist. IDs in behavior that are consistent across time and context are more recently recogn...

journal_title：Zebrafish

authors： Toms CN,Echevarria DJ

更新日期：2014-08-01 00:00:00

abstract：:Dimethyl sulfoxide (DMSO), a widely used carrier solvent, can be toxic to test organisms and has species-specific sensitivity. In this study, the developmental toxicity and stress protein responses of DMSO to rare minnow (Gobiocypris rarus) and zebrafish (Danio rerio) with two tests were compared in the early life sta...

journal_title：Zebrafish

authors： Xiong X,Luo S,Wu B,Wang J

更新日期：2017-02-01 00:00:00

abstract：:An emergent field of animal personality necessitates a method for repeated high-throughput quantification of behavioral traits across contexts. In this study, we have developed an automated video stimulus approach to sequentially present different contexts relevant to five "personality" traits (exploration, boldness, ...

journal_title：Zebrafish

authors： Fangmeier ML,Noble DWA,O'Dea RE,Usui T,Lagisz M,Hesselson D,Nakagawa S

更新日期：2018-04-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has been applied to edit genomes in a wide variety of model systems. Although this process can be quite efficient, editing at precise locations in the genome remains difficult without a suitable single guide RNA (sgRNA). We have developed a method...

journal_title：Zebrafish

authors： Grainger S,Lonquich B,Oon CH,Nguyen N,Willert K,Traver D

更新日期：2017-08-01 00:00:00

abstract：:Many facilities house fish in separate static containers post-procedure, for example, while awaiting genotyping results. This ensures fish can be easily identified, but it does not allow for provision of continuous filtered water or diet. At the Wellcome Trust Sanger Institute, concern over the housing conditions led ...

journal_title：Zebrafish

authors： Goodwin N,Westall L,Karp NA,Hazlehurst D,Kovacs C,Keeble R,Thompson P,Collins R,Bussell J

更新日期：2016-07-01 00:00:00

abstract：:With worldwide rates of obesity and type-II diabetes increasing, it is essential to identify and understand the mechanisms involved during nutrient absorption and fuel allocation. Recent studies demonstrate that nutrients (e.g., lipids and carbohydrates) play a major regulatory role in gene transcription of glycolytic...

journal_title：Zebrafish

authors： Craig PM,Moon TW

更新日期：2011-09-01 00:00:00