Methods for culturing saltwater rotifers (Brachionus plicatilis) for rearing larval zebrafish.

Abstract:

:The saltwater rotifer, Brachionus plicatilis, is widely used in the aquaculture industry as a prey item for first-feeding fishes due to its ease of culture, small size, rapid reproductive rate, and amenability to enrichment with nutrients. Despite the distinct advantages of this approach, rotifers have only been sporadically utilized for rearing larval zebrafish, primarily because of the common misconception that maintaining cultures of rotifers is difficult and excessively time-consuming. Here we present simple methods for maintaining continuous cultures of rotifers capable of supporting even the very largest zebrafish aquaculture facility, with minimal investments in materials, time, labor, and space. Examples of the methods' application in one large, existing facility is provided, and troubleshooting of common problems is discussed.

journal_name

Zebrafish

journal_title

Zebrafish

authors

Lawrence C,Sanders E,Henry E

doi

10.1089/zeb.2012.0771

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

140-6

issue

3

eissn

1545-8547

issn

1557-8542

journal_volume

9

pub_type

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