Abstract:
:Traditional working heart preparations, based on the original Langendorff setup, are widely used experimental models that have tremendously advanced the cardiovascular field. However, these systems can be deceivingly complex, requiring the maintenance of pH with CO(2), the delivery of oxygenated perfusate, and the need for extensive laboratory equipment. We have examined the feasibility of using isolated zebrafish (Danio rerio) hearts as an experimental model system, in which experimental procedures can be performed in the absence of the traditional requirements and sophisticated setup equipment. Isolated zebrafish hearts exhibited spontaneous contractile activity, could be electrically paced, and were responsive to pharmacologic stimulation with isoproterenol for 1.5 h after in vivo removal. Isolated zebrafish hearts offer a time- and cost-effective alternative to traditional Langendorff/working heart preparation models, and could be used to investigate cardiac function and repair.
journal_name
Zebrafishjournal_title
Zebrafishauthors
Hecker L,Khait L,Sessions SK,Birla RKdoi
10.1089/zeb.2008.0550subject
Has Abstractpub_date
2008-12-01 00:00:00pages
319-22issue
4eissn
1545-8547issn
1557-8542pii
10.1089/zeb.2008.0550journal_volume
5pub_type
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