Abstract:
:The completion of the zebrafish genome sequence and advances in miniaturization and multiplexing were essential to the creation of techniques such as RNA-seq, ChIP-seq, and high-throughput behavioral and chemical screens. Multiplexing was also instrumental in the recent enhancement of the classic yeast one-hybrid interaction techniques to provide unprecedented discovery capabilities for protein-DNA interactions. Unfortunately its use for zebrafish research is currently hampered by the lack of an open reading frame (ORF) clone collection. As a first step toward a complete collection, we describe a small library of transcriptional regulatory proteins comprising 142 ORFs and its potential applications.
journal_name
Zebrafishjournal_title
Zebrafishauthors
King J,Foster J,Davison JM,Rawls JF,Breton Gdoi
10.1089/zeb.2017.1486subject
Has Abstractpub_date
2018-04-01 00:00:00pages
202-205issue
2eissn
1545-8547issn
1557-8542journal_volume
15pub_type
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