A Simple Method for Immunohistochemical Staining of Zebrafish Brain Sections for c-fos Protein Expression.


:Immediate early genes (IEGs) are transcription factors whose own transcription is initiated rapidly, for example, in the brain in response to environmental stimuli. c-fos is an IEG often used as a marker of neuronal activation. c-fos mRNA expression has started to be quantified and localized in the zebrafish brain following environmental manipulations but analysis of the expression of c-fos protein in the zebrafish brain has rarely been attempted. Here, we describe an immunofluorescence staining method for quantifying c-fos protein expression in different regions of the zebrafish brain. In addition, we expose zebrafish to caffeine, a positive control for c-fos activation in the brain. To confirm cell nucleus specific binding of the c-fos antibody, we counterstained brain sections with the nuclear fluorescent stain DAPI. Furthermore, we describe a method for reducing background autofluorescence often observed in zebrafish brain tissue. Our analysis showed that exposure to caffeine increased the number of c-fos protein-positive cells in specific zebrafish brain regions detected by the immunofluorescence method. Our results demonstrate the feasibility of immunofluorescence-based methods in the analysis of neuronal activation in the zebrafish brain, and reinforce the utility of the zebrafish in behavioral neuroscience research.






Chatterjee D,Tran S,Shams S,Gerlai R




Has Abstract


2015-12-01 00:00:00












  • Zebrafish in the wild: a review of natural history and new notes from the field.

    abstract::The zebrafish, Danio rerio, has emerged as a major model organism for biomedical research, yet little is known about its natural history. We review the literature pertaining to the geographic range, biotic and abiotic habitats, and life cycle of the zebrafish. We also report our own field study to document several asp...


    pub_type: 杂志文章,评审


    authors: Engeszer RE,Patterson LB,Rao AA,Parichy DM

    更新日期:2007-04-01 00:00:00

  • Influence of Long-Term Feeding Antibiotics on the Gut Health of Zebrafish.

    abstract::The use of antibiotics for anti-infection and growth promotion has caused the overuse of antibiotics in aquaculture. However, the benefit or risk of the long-term use of antibiotics on fish growth or health has not been fully addressed. In the present study, zebrafish were fed with sulfamethoxazole (SMX) or oxytetracy...


    pub_type: 杂志文章


    authors: Zhou L,Limbu SM,Qiao F,Du ZY,Zhang M

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  • An Open-Source Husbandry Repository.

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    pub_type: 杂志文章


    authors: LaCoursiere CM,Ullmann JFP,Poduri A

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  • Zebrafish (Danio rerio) as a Model for Understanding the Process of Caudal Fin Regeneration.

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    pub_type: 杂志文章


    authors: Lebedeva L,Zhumabayeva B,Gebauer T,Kisselev I,Aitasheva Z

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  • Chromosomal Diversification Higher Than Molecular Variation in Astyanax aff. fasciatus (Teleostei, Characidae).

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    pub_type: 杂志文章


    authors: Kavalco KF,Pazza R,Brandão Kde O,Garcia C,Bertollo LA,de Almeida-Toledo LF

    更新日期:2016-08-01 00:00:00

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    pub_type: 面试


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    authors: Lopes SS,Distel M,Linker C,Fior R,Monteiro R,Bianco IH,Portugues R,Strähle U,Saúde L

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    pub_type: 杂志文章


    authors: Hecker L,Khait L,Sessions SK,Birla RK

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Yossa R,Sarker PK,Vandenberg GW

    更新日期:2011-12-01 00:00:00

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    authors: Api M,Biondi P,Olivotto I,Terzibasi E,Cellerino A,Carnevali O

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    pub_type: 杂志文章


    authors: Antoine TE,Jones KS,Dale RM,Shukla D,Tiwari V

    更新日期:2014-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Earley RL

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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Clay H,Ramakrishnan L

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Cardoso AL,Carvalho HL,Benathar TC,Serrão SM,Nagamachi CY,Pieczarka JC,Sousa LM,Ready JS,Noronha RC

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Parker MO,Brock AJ,Millington ME,Brennan CH

    更新日期:2013-12-01 00:00:00