Abstract:
:We have developed a simple method for the induction of cataracts in adult zebrafish using hydrogen peroxide. Our method uses a corneal puncture followed by intraocular injection of hydrogen peroxide into the anterior chamber. The procedure can be done rapidly, forming a highly opaque cataract almost immediately, and fish recover quickly. The optical clarity of the lens is gradually regained over time, showing the innate ability of zebrafish to regenerate healthy lens tissue. This technique provides a simple in vivo system with which to assess mutant strains or targeted disruptions to genes expressed in the lens.
journal_name
Zebrafishjournal_title
Zebrafishauthors
Prior HM,Letwin K,Tuininga A,Nguyen Mdoi
10.1089/zeb.2017.1533subject
Has Abstractpub_date
2018-04-01 00:00:00pages
211-212issue
2eissn
1545-8547issn
1557-8542journal_volume
15pub_type
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