Cancer cytogenetics in the zebrafish.

abstract：:Most young researchers leaving the safe haven of postdoctoral life for the unchartered territory of a PI position are confronted with a multitude of new tasks. For those using fish as their favorite model system, this often includes the daunting task of setting up and running a fish facility. New PIs are expected to k...

journal_title：Zebrafish

authors： McNabb A,Scott K,Ochsenstein Ev,Seufert K,Carl M

更新日期：2012-09-01 00:00:00

abstract：:Understanding living systems requires an in-depth knowledge of the signaling networks that drive cellular homeostasis, regulate intercellular communication, and contribute to cell fates during development. Several resources exist to provide high-throughput data sets or manually curated interaction information from hum...

journal_title：Zebrafish

authors： Csályi K,Fazekas D,Kadlecsik T,Türei D,Gul L,Horváth B,Módos D,Demeter A,Pápai N,Lenti K,Csermely P,Vellai T,Korcsmáros T,Varga M

更新日期：2016-12-01 00:00:00

abstract：:The zebrafish is a valuable model for teaching developmental, molecular, and cell biology; aquatic sciences; comparative anatomy; physiology; and genetics. Here we demonstrate that zebrafish provide an excellent model system to teach engineering principles. A seven-member undergraduate team in a biomedical engineering...

journal_title：Zebrafish

authors： Shen YC,Li D,Al-Shoaibi A,Bersano-Begey T,Chen H,Ali S,Flak B,Perrin C,Winslow M,Shah H,Ramamurthy P,Schmedlen RH,Takayama S,Barald KF

更新日期：2009-06-01 00:00:00

abstract：:The two LIM kinases (LIMKs) LIMK1 and LIMK2 are members of the PDZ/LIM family. These serine/threonine protein kinases are involved in actin cytoskeleton reorganization through phosphorylation and inactivation of ADF/cofilin. Different subcellular localizations of LIMK1 and LIMK2 suggest different functions. LIMK1 is i...

journal_title：Zebrafish

authors： Vlecken DH,Bagowski CP

更新日期：2009-12-01 00:00:00

abstract：:To promote application of the fruits of zebrafish research, we need to cultivate understanding of the use of zebrafish not only in the research community but also among the general public. On Open Days at our institute, the National Institute for Environmental Studies (NIES), our zebrafish group exhibits our research ...

journal_title：Zebrafish

authors： Aoki Y

更新日期：2009-06-01 00:00:00

abstract：:The zebrafish had landed in Singapore as an ornamental fish long before it became a fashionable model of scientific research. By the early 1990s, however, it became a part of the local scientific landscape. During the past decade the number of groups using zebrafish as a research model increased dramatically. In June ...

journal_title：Zebrafish

authors： You MS,Korzh V

更新日期：2005-01-01 00:00:00

abstract：:Astyanax is a genus with a wide distribution ranging from the south United States to north of Patagonia (Argentina). The available cytogenetic data on Astyanax indicate a high karyotypic diversity, with diploid number of 36-52 chromosomes, presence of B chromosomes, heterochromatin polymorphism, and variations with re...

journal_title：Zebrafish

authors： Piscor D,Pozzobon APB,Fernandes CA,Centofante L,Parise-Maltempi PP

更新日期：2019-02-01 00:00:00

abstract：:Dr. Zhiyuan Gong is Associate Professor in the Department of Biological Sciences, National University of Singapore. He graduated with a B.Sc. degree in marine biology from Ocean University of China and received his Ph.D. degree in developmental biology from McGill University in Canada. He spent many years at the Unive...

journal_title：Zebrafish

authors： Gong Z

更新日期：2004-01-01 00:00:00

abstract：:Zebrafish embryos are widely used as a model to monitor the effect of chemicals on their survival and hatching at different time epochs. This experimental design generates longitudinal data in which the observations for a given subject are correlated and they are statistically independent across the subjects. This par...

journal_title：Zebrafish

authors： Aparna C,Sanjeev S,Ajit J,Shantaram K,Jayesh B,Suresh AK

更新日期：2014-08-01 00:00:00

abstract：:The European Zebrafish Principal Investigator Meeting (EZPM) is an ideal forum for group leaders using this fantastic animal model not only to discuss science but also to strengthen their interactions, to push forward technological advances, and to define guidelines for the use of this fish in research. The city of Li...

journal_title：Zebrafish

authors： Lopes SS,Distel M,Linker C,Fior R,Monteiro R,Bianco IH,Portugues R,Strähle U,Saúde L

更新日期：2016-12-01 00:00:00

abstract：:The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacen...

journal_title：Zebrafish

authors： Alves-Silva AP,Dergam JA

更新日期：2015-02-01 00:00:00

abstract：:This study reports the first production of offspring with vitrified sperm from a live-bearing fish Xiphophorus hellerii. The overall goal of this study was to develop streamlined protocols for integration into a standardized approach for vitrification of aquatic species germplasm. The objectives were to (1) estimate a...

journal_title：Zebrafish

authors： Cuevas-Uribe R,Yang H,Daly J,Savage MG,Walter RB,Tiersch TR

更新日期：2011-12-01 00:00:00

abstract：:A husbandry workshop on July 3, 2017, at the 10th European Zebrafish Meeting in Budapest, Hungary (July 3-July 7, 2017), focused on the standardization, optimization, and streamlining of fish facility procedures. Standardization can be achieved for example by developing novel software and hardware tools, such as a fis...

journal_title：Zebrafish

authors： Oltová J,Barton C,Certal AC,Argenton F,Varga ZM

更新日期：2018-04-01 00:00:00

abstract：:Pamela Raymond received her B.S., M.S., and Ph.D. degrees from the University of Michigan. She is currently Professor of Molecular, Cellular, and Developmental Biology in the College of Literature, Science, and the Arts at the University of Michigan. She has held academic appointments at Harvard Medical School and the...

journal_title：Zebrafish

authors： Raymond P

更新日期：2005-01-01 00:00:00

abstract：:The genetic compensation response (GCR), triggered by deleterious mutations but not by gene knockdown, has been proposed to explain many phenotypic discrepancies between gene-knockout and gene-knockdown models. GCRs have been observed in many model organisms from mice to Arabidopsis. Although the GCR is beneficial for...

journal_title：Zebrafish

authors： Ma Z,Chen J

更新日期：2020-05-20 00:00:00

abstract：:Microcystin-leucine arginine (MC-LR) is a natural toxin produced by cyanobacterial blooms that causes severe liver damage in fish. It is crucial to investigate if housekeeping genes are affected by MC-LR in zebrafish, to permit the adequate evaluation of gene expression by RT-qPCR. We evaluated the gene expression sta...

journal_title：Zebrafish

authors： Sopezki MDS,Monserrat JM,Yunes JS,Zanette J

更新日期：2018-10-01 00:00:00

abstract：:We have shown that zebrafish (Danio rerio) are an excellent model for evaluating the link between early life stage exposure to environmental chemicals and disease in adulthood and subsequent unexposed generations. Previously, we used this model to identify transgenerational effects of dioxin (2,3,7,8-tetrachlorodibenz...

journal_title：Zebrafish

authors： Baker BB,Yee JS,Meyer DN,Yang D,Baker TR

更新日期：2016-10-01 00:00:00

abstract：:Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to mi...

journal_title：Zebrafish

authors： Bill BR,Petzold AM,Clark KJ,Schimmenti LA,Ekker SC

更新日期：2009-03-01 00:00:00

abstract：:With worldwide rates of obesity and type-II diabetes increasing, it is essential to identify and understand the mechanisms involved during nutrient absorption and fuel allocation. Recent studies demonstrate that nutrients (e.g., lipids and carbohydrates) play a major regulatory role in gene transcription of glycolytic...

journal_title：Zebrafish

authors： Craig PM,Moon TW

更新日期：2011-09-01 00:00:00

abstract：:The Johns Hopkins Clinical Compound Library (JHCCL), a collection of Food and Drug Administration (FDA)-approved small molecules (1400), was screened in silico for identification of novel β2AR blockers and tested for hematopoietic stem cell (HSC) expansion and radioprotection in zebrafish embryos. Docking studies, fol...

journal_title：Zebrafish

authors： Dimri M,Joshi J,Chakrabarti R,Sehgal N,Sureshbabu A,Kumar IP

更新日期：2015-02-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has been applied to edit genomes in a wide variety of model systems. Although this process can be quite efficient, editing at precise locations in the genome remains difficult without a suitable single guide RNA (sgRNA). We have developed a method...

journal_title：Zebrafish

authors： Grainger S,Lonquich B,Oon CH,Nguyen N,Willert K,Traver D

更新日期：2017-08-01 00:00:00

abstract：:We are entering a new era in our ability to modify and edit the genomes of model organisms. Zinc finger nucleases (ZFNs) opened the door to the first custom nuclease-targeted genome engineering in the late 1990s. However, ZFNs remained out of reach for most research labs because of the difficulty of production, high c...

journal_title：Zebrafish

authors： Blackburn PR,Campbell JM,Clark KJ,Ekker SC

更新日期：2013-03-01 00:00:00

abstract：:Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this st...

journal_title：Zebrafish

authors： De Rienzo G,Gutzman JH,Sive H

更新日期：2012-09-01 00:00:00

abstract：:In an effort to engage students in original research while teaching them basic molecular biology skills, we have designed a course for upper level undergraduate students and beginning graduate students that employs in situ hybridization in whole-mount zebrafish embryos to explore the concept of differential gene regul...

journal_title：Zebrafish

authors： Schmoldt A,Forecki J,Hammond DR,Udvadia AJ

更新日期：2009-06-01 00:00:00

abstract：:For many years, zebrafish have been the prototypical model for studies in developmental biology. In recent years, zebrafish has emerged as a powerful model system to study infectious diseases, including viral infections. Experiments conducted with herpes simplex virus type-1 in adult zebrafish or in embryo models are ...

journal_title：Zebrafish

authors： Antoine TE,Jones KS,Dale RM,Shukla D,Tiwari V

更新日期：2014-02-01 00:00:00

abstract：:Dimethyl sulfoxide (DMSO), a widely used carrier solvent, can be toxic to test organisms and has species-specific sensitivity. In this study, the developmental toxicity and stress protein responses of DMSO to rare minnow (Gobiocypris rarus) and zebrafish (Danio rerio) with two tests were compared in the early life sta...

journal_title：Zebrafish

authors： Xiong X,Luo S,Wu B,Wang J

更新日期：2017-02-01 00:00:00

abstract：:β-Cells of the islet of Langerhans produce insulin to maintain glucose homeostasis. Self-replication of β-cells is the predominant mode of postnatal β-cell production in mammals, with about 20% of rodent β cells dividing in a 24-hour period. However, replicating β-cells are rare in adults. Induction of self-replicatio...

journal_title：Zebrafish

authors： Li M,Maddison LA,Crees Z,Chen W

更新日期：2013-06-01 00:00:00

abstract：:The zebrafish has considerable potential for use as a model in the study of behavior in social systems, particularly dominance hierarchies, which are widespread in nature and can affect the lifelong success of individuals. There is, however, a paucity of information relating to the characterization of social groups an...

journal_title：Zebrafish

authors： Paull GC,Filby AL,Giddins HG,Coe TS,Hamilton PB,Tyler CR

更新日期：2010-03-01 00:00:00

abstract：:Electronic databases provide effective and efficient management of zebrafish colony operations, but commercially available options are expensive. In this study we have developed a free zebrafish management repository alternative using free Google applications. Husbandry information is logged into a Google Sheets-based...

journal_title：Zebrafish

authors： LaCoursiere CM,Ullmann JFP,Poduri A

更新日期：2018-12-01 00:00:00

abstract：:A review of some exciting new directions for Xiphophorus research in behavioral ecology and integrative animal behavior is presented. The primary objective of this review is to identify areas of research in this teleost genus that, if studied further, could yield insights into the manifold causes and consequences of d...

journal_title：Zebrafish

authors： Earley RL

更新日期：2006-01-01 00:00:00