Validation of Housekeeping Genes as Internal Controls for the Study of the Effects of Microcystin-LR in Zebrafish by Real-Time PCR.

abstract：:Immediate early genes (IEGs) are transcription factors whose own transcription is initiated rapidly, for example, in the brain in response to environmental stimuli. c-fos is an IEG often used as a marker of neuronal activation. c-fos mRNA expression has started to be quantified and localized in the zebrafish brain fol...

journal_title：Zebrafish

authors： Chatterjee D,Tran S,Shams S,Gerlai R

更新日期：2015-12-01 00:00:00

abstract：:Traditional working heart preparations, based on the original Langendorff setup, are widely used experimental models that have tremendously advanced the cardiovascular field. However, these systems can be deceivingly complex, requiring the maintenance of pH with CO(2), the delivery of oxygenated perfusate, and the nee...

journal_title：Zebrafish

authors： Hecker L,Khait L,Sessions SK,Birla RK

更新日期：2008-12-01 00:00:00

abstract：:During the last few years zebrafish and other aquarium fish have become more important model system species. In zebrafish valuable biotechnological lines are constantly emerging, and the creation of cryobanks of viable fish sperm, oocytes, and embryos from those lines is a constant and important demand. Cryopreservati...

journal_title：Zebrafish

authors： Robles V,Cabrita E,Herráez MP

更新日期：2009-09-01 00:00:00

abstract：:The zebrafish is a valuable model for teaching developmental, molecular, and cell biology; aquatic sciences; comparative anatomy; physiology; and genetics. Here we demonstrate that zebrafish provide an excellent model system to teach engineering principles. A seven-member undergraduate team in a biomedical engineering...

journal_title：Zebrafish

authors： Shen YC,Li D,Al-Shoaibi A,Bersano-Begey T,Chen H,Ali S,Flak B,Perrin C,Winslow M,Shah H,Ramamurthy P,Schmedlen RH,Takayama S,Barald KF

更新日期：2009-06-01 00:00:00

abstract：:Dr. Zhiyuan Gong is Associate Professor in the Department of Biological Sciences, National University of Singapore. He graduated with a B.Sc. degree in marine biology from Ocean University of China and received his Ph.D. degree in developmental biology from McGill University in Canada. He spent many years at the Unive...

journal_title：Zebrafish

authors： Gong Z

更新日期：2004-01-01 00:00:00

abstract：:β-Cells of the islet of Langerhans produce insulin to maintain glucose homeostasis. Self-replication of β-cells is the predominant mode of postnatal β-cell production in mammals, with about 20% of rodent β cells dividing in a 24-hour period. However, replicating β-cells are rare in adults. Induction of self-replicatio...

journal_title：Zebrafish

authors： Li M,Maddison LA,Crees Z,Chen W

更新日期：2013-06-01 00:00:00

abstract：:The diversity of Hypancistrus species in the Xingu River is remarkable and the variation in color morphs represents a real challenge to taxonomists to delimit species boundaries. One of the most recognizable Hypancistrus complexes is the worm-lined species, known in the aquarium trade as King Tiger Plec in English, Hy...

journal_title：Zebrafish

authors： Cardoso AL,Carvalho HL,Benathar TC,Serrão SM,Nagamachi CY,Pieczarka JC,Sousa LM,Ready JS,Noronha RC

更新日期：2016-06-01 00:00:00

abstract：:Electronic databases provide effective and efficient management of zebrafish colony operations, but commercially available options are expensive. In this study we have developed a free zebrafish management repository alternative using free Google applications. Husbandry information is logged into a Google Sheets-based...

journal_title：Zebrafish

authors： LaCoursiere CM,Ullmann JFP,Poduri A

更新日期：2018-12-01 00:00:00

abstract：:Although zebrafish is used as a major model species for understanding a number of biological functions and mechanisms involved, there is practically no information on the nutritional requirements of this cyprinid. The rearing practices, especially with regard to feeds and feeding, also vary considerably. To obtain bas...

journal_title：Zebrafish

authors： Kaushik S,Georga I,Koumoundouros G

更新日期：2011-06-01 00:00:00

abstract：:The ability to visualise neural circuits in zebrafish in vivo is one of the most useful aspects of this model organism in neuroscience. To maintain the transparency of embryos, however, drugs, such as 1-pheyl-2-thiourea (PTU) must be added, or researchers can use mutants that do not develop pigment (e.g., the casper)....

journal_title：Zebrafish

authors： Parker MO,Brock AJ,Millington ME,Brennan CH

更新日期：2013-12-01 00:00:00

abstract：:Comprising a large number of species, the genus Astyanax has been intensively studied by several approaches to elucidate its evolutionary relationships. Such studies have demonstrated that many nominal species are artificial clusters where distinct taxa are grouped under the same denomination. Astyanax aff. fasciatus ...

journal_title：Zebrafish

authors： Kavalco KF,Pazza R,Brandão Kde O,Garcia C,Bertollo LA,de Almeida-Toledo LF

更新日期：2016-08-01 00:00:00

abstract：:The genetic compensation response (GCR), triggered by deleterious mutations but not by gene knockdown, has been proposed to explain many phenotypic discrepancies between gene-knockout and gene-knockdown models. GCRs have been observed in many model organisms from mice to Arabidopsis. Although the GCR is beneficial for...

journal_title：Zebrafish

authors： Ma Z,Chen J

更新日期：2020-05-20 00:00:00

abstract：:For many years, zebrafish have been the prototypical model for studies in developmental biology. In recent years, zebrafish has emerged as a powerful model system to study infectious diseases, including viral infections. Experiments conducted with herpes simplex virus type-1 in adult zebrafish or in embryo models are ...

journal_title：Zebrafish

authors： Antoine TE,Jones KS,Dale RM,Shukla D,Tiwari V

更新日期：2014-02-01 00:00:00

abstract：:After its introduction for scientific investigation in the 1950s, the cypriniform zebrafish, Danio rerio, has become a valuable model for the study of regenerative processes and mechanisms. Zebrafish exhibit epimorphic regeneration, in which a nondifferentiated cell mass formed after amputation is able to fully regene...

journal_title：Zebrafish

authors： Lebedeva L,Zhumabayeva B,Gebauer T,Kisselev I,Aitasheva Z

更新日期：2020-12-01 00:00:00

abstract：:The monotypic Astroblepidae fish family includes 81 species distributed along Central and Northern Andes in South America and Panamá in Central America; most aspects regarding its biology, taxonomy, and chromosomal features remain largely unknown. This study reports the karyotype of two sympatric Astroblepus species f...

journal_title：Zebrafish

authors： Conde-Saldaña CC,Cunha MS,Albornoz-Garzón JG,Barreto CAV,Ibagón N,Villa-Navarro FA,Dergam JA

更新日期：2019-02-01 00:00:00

abstract：:This study reports the first production of offspring with vitrified sperm from a live-bearing fish Xiphophorus hellerii. The overall goal of this study was to develop streamlined protocols for integration into a standardized approach for vitrification of aquatic species germplasm. The objectives were to (1) estimate a...

journal_title：Zebrafish

authors： Cuevas-Uribe R,Yang H,Daly J,Savage MG,Walter RB,Tiersch TR

更新日期：2011-12-01 00:00:00

abstract：:Mycobacteriosis is a common bacterial infection in laboratory zebrafish caused by several different species and strains of Mycobacterium, including both rapid and slow growers. One control measure used to prevent mycobacterial spread within and between facilities is surface disinfection of eggs. Recent studies have hi...

journal_title：Zebrafish

authors： Chang CT,Amack JD,Whipps CM

更新日期：2016-07-01 00:00:00

abstract：:The Cannabis sativa plant contains numerous phytocannabinoids and terpenes with known or potential biological activity. For decades, plant breeders have been breeding the Cannabis plant to control for a desired ratio of the major cannabinoids. A high-throughput in vivo model to understand the relationship between the ...

journal_title：Zebrafish

authors： Achenbach JC,Hill J,Hui JPM,Morash MG,Berrue F,Ellis LD

更新日期：2018-08-01 00:00:00

abstract：:Wild zebrafish exhibit a wide range of behavior. We found abundant wild zebrafish in flowing rivers and still water, in large, tightly-knit groups of hundreds of individuals, as well as in small, loose shoals. In two still-water populations, zebrafish were quite small in body size, common, and in tight groups of up to...

journal_title：Zebrafish

authors： Suriyampola PS,Shelton DS,Shukla R,Roy T,Bhat A,Martins EP

更新日期：2016-02-01 00:00:00

abstract：:The use of antibiotics for anti-infection and growth promotion has caused the overuse of antibiotics in aquaculture. However, the benefit or risk of the long-term use of antibiotics on fish growth or health has not been fully addressed. In the present study, zebrafish were fed with sulfamethoxazole (SMX) or oxytetracy...

journal_title：Zebrafish

authors： Zhou L,Limbu SM,Qiao F,Du ZY,Zhang M

更新日期：2018-08-01 00:00:00

abstract：:Zebrafish are increasingly used in neurobiological and behavioral studies. Possible stimuli to manipulate zebrafish behavior are being investigated. The presentation of colors appears to be one of the most used approaches, but there is much debate about the exact color preference. Here we have investigated the color p...

journal_title：Zebrafish

authors： Peeters BW,Moeskops M,Veenvliet AR

更新日期：2016-08-01 00:00:00

abstract：:The second European Zebrafish Principal Investigator (PI) Meeting was held in March, 2012, in Karlsruhe, Germany. It brought together PIs from all over Europe who work with fish models such as zebrafish and medaka to discuss their latest results, as well as to resolve strategic issues faced by this research community....

journal_title：Zebrafish

authors： Cavodeassi F,Del Bene F,Fürthauer M,Grabher C,Herzog W,Lehtonen S,Linker C,Mercader N,Mikut R,Norton W,Strähle U,Tiso N,Foulkes NS

更新日期：2013-03-01 00:00:00

abstract：:Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DN...

journal_title：Zebrafish

authors： Kuriya K,Higashiyama E,Avşar-Ban E,Tamaru Y,Ogata S,Takebayashi S,Ogata M,Okumura K

更新日期：2015-12-01 00:00:00

abstract：:There is now strong evidence that early nutrition plays an important role in shaping later physiology. We assessed here whether soy protein concentrate (SPC) or soybean meal (SBM) in early diet would modify zebrafish responses to these products in later life. We fed zebrafish larvae with SPC-, SBM-, or a control-diet ...

journal_title：Zebrafish

authors： Perera E,Yúfera M

更新日期：2016-02-01 00:00:00

abstract：:The completion of the zebrafish genome sequence and advances in miniaturization and multiplexing were essential to the creation of techniques such as RNA-seq, ChIP-seq, and high-throughput behavioral and chemical screens. Multiplexing was also instrumental in the recent enhancement of the classic yeast one-hybrid inte...

journal_title：Zebrafish

authors： King J,Foster J,Davison JM,Rawls JF,Breton G

更新日期：2018-04-01 00:00:00

abstract：:Dimethyl sulfoxide (DMSO), a widely used carrier solvent, can be toxic to test organisms and has species-specific sensitivity. In this study, the developmental toxicity and stress protein responses of DMSO to rare minnow (Gobiocypris rarus) and zebrafish (Danio rerio) with two tests were compared in the early life sta...

journal_title：Zebrafish

authors： Xiong X,Luo S,Wu B,Wang J

更新日期：2017-02-01 00:00:00

abstract：:The Johns Hopkins Clinical Compound Library (JHCCL), a collection of Food and Drug Administration (FDA)-approved small molecules (1400), was screened in silico for identification of novel β2AR blockers and tested for hematopoietic stem cell (HSC) expansion and radioprotection in zebrafish embryos. Docking studies, fol...

journal_title：Zebrafish

authors： Dimri M,Joshi J,Chakrabarti R,Sehgal N,Sureshbabu A,Kumar IP

更新日期：2015-02-01 00:00:00

abstract：:Considering the numbers of zebrafish held in the laboratories, it is relevant to develop some tools to monitor the health of the animals, as well as their biotope. Environmental samples can be used to detect aquatic pathogens. Comprehensive health monitoring would thus seek pathogens in three dimensions of the animals...

journal_title：Zebrafish

authors： Mocho JP

更新日期：2016-07-01 00:00:00

abstract：:We have constructed a low-cost aquatic animal holding system that provides an alternative to expensive, commercially available systems. Our flow-through system is especially useful for programs that are limited in space and funding. The easy assembly and maintenance of the system are advantages for the researchers who...

journal_title：Zebrafish

authors： Hohn C,Petrie-Hanson L

更新日期：2007-07-01 00:00:00

abstract：:The zebrafish system has been established as a useful model for the study of carcinogenesis. The cytogenetic characterization of the genome is vital for furthering our understanding of the progression of the disease. Establishing a basic description of the zebrafish chromosomal karyotype and markers for each specific ...

journal_title：Zebrafish

authors： Peterson SM,Freeman JL

更新日期：2009-12-01 00:00:00