Evaluating and Optimizing Fish Health and Welfare During Experimental Procedures.

abstract：:Astyanax is a genus with a wide distribution ranging from the south United States to north of Patagonia (Argentina). The available cytogenetic data on Astyanax indicate a high karyotypic diversity, with diploid number of 36-52 chromosomes, presence of B chromosomes, heterochromatin polymorphism, and variations with re...

journal_title：Zebrafish

authors： Piscor D,Pozzobon APB,Fernandes CA,Centofante L,Parise-Maltempi PP

更新日期：2019-02-01 00:00:00

abstract：:Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to mi...

journal_title：Zebrafish

authors： Bill BR,Petzold AM,Clark KJ,Schimmenti LA,Ekker SC

更新日期：2009-03-01 00:00:00

abstract：:Zebrafish (Danio rerio) are rapidly gaining popularity in translational neuroscience and behavioral research. Physiological similarity to mammals, ease of genetic manipulations, sensitivity to pharmacological and genetic factors, robust behavior, low cost, and potential for high-throughput screening contribute to the ...

journal_title：Zebrafish

authors： Kalueff AV,Gebhardt M,Stewart AM,Cachat JM,Brimmer M,Chawla JS,Craddock C,Kyzar EJ,Roth A,Landsman S,Gaikwad S,Robinson K,Baatrup E,Tierney K,Shamchuk A,Norton W,Miller N,Nicolson T,Braubach O,Gilman CP,Pittman J

更新日期：2013-03-01 00:00:00

abstract：:The monotypic Astroblepidae fish family includes 81 species distributed along Central and Northern Andes in South America and Panamá in Central America; most aspects regarding its biology, taxonomy, and chromosomal features remain largely unknown. This study reports the karyotype of two sympatric Astroblepus species f...

journal_title：Zebrafish

authors： Conde-Saldaña CC,Cunha MS,Albornoz-Garzón JG,Barreto CAV,Ibagón N,Villa-Navarro FA,Dergam JA

更新日期：2019-02-01 00:00:00

abstract：:For many years, zebrafish have been the prototypical model for studies in developmental biology. In recent years, zebrafish has emerged as a powerful model system to study infectious diseases, including viral infections. Experiments conducted with herpes simplex virus type-1 in adult zebrafish or in embryo models are ...

journal_title：Zebrafish

authors： Antoine TE,Jones KS,Dale RM,Shukla D,Tiwari V

更新日期：2014-02-01 00:00:00

abstract：:Immediate early genes (IEGs) are transcription factors whose own transcription is initiated rapidly, for example, in the brain in response to environmental stimuli. c-fos is an IEG often used as a marker of neuronal activation. c-fos mRNA expression has started to be quantified and localized in the zebrafish brain fol...

journal_title：Zebrafish

authors： Chatterjee D,Tran S,Shams S,Gerlai R

更新日期：2015-12-01 00:00:00

abstract：:The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacen...

journal_title：Zebrafish

authors： Alves-Silva AP,Dergam JA

更新日期：2015-02-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has been applied to edit genomes in a wide variety of model systems. Although this process can be quite efficient, editing at precise locations in the genome remains difficult without a suitable single guide RNA (sgRNA). We have developed a method...

journal_title：Zebrafish

authors： Grainger S,Lonquich B,Oon CH,Nguyen N,Willert K,Traver D

更新日期：2017-08-01 00:00:00

abstract：:A husbandry workshop on July 3, 2017, at the 10th European Zebrafish Meeting in Budapest, Hungary (July 3-July 7, 2017), focused on the standardization, optimization, and streamlining of fish facility procedures. Standardization can be achieved for example by developing novel software and hardware tools, such as a fis...

journal_title：Zebrafish

authors： Oltová J,Barton C,Certal AC,Argenton F,Varga ZM

更新日期：2018-04-01 00:00:00

abstract：:Since the use of the zebrafish Danio rerio genetic model organism within the scientific research community continues to grow rapidly, continued procedural refinement to support high-quality, reproducible research and improve animal welfare remains an important focus. As such, anesthesia remains one of the most frequen...

journal_title：Zebrafish

authors： Ehrlich O,Karamalakis A,Krylov AJ,Dudczig S,Hassell KL,Jusuf PR

更新日期：2019-10-01 00:00:00

abstract：:Managing the welfare of laboratory animals is critical to animal health, vital in the understanding of phenotypes created by treatment or genetic alteration and ensures compliance of regulations. Part of an animal welfare assessment is the requirement to record observations, ensuring all those responsible for the anim...

journal_title：Zebrafish

authors： Goodwin N,Karp NA,Blackledge S,Clark B,Keeble R,Kovacs C,Murray KN,Price M,Thompson P,Bussell J

更新日期：2016-07-01 00:00:00

abstract：:The zebrafish, Danio rerio, has emerged as a major model organism for biomedical research, yet little is known about its natural history. We review the literature pertaining to the geographic range, biotic and abiotic habitats, and life cycle of the zebrafish. We also report our own field study to document several asp...

journal_title：Zebrafish

authors： Engeszer RE,Patterson LB,Rao AA,Parichy DM

更新日期：2007-04-01 00:00:00

abstract：:The completion of the zebrafish genome sequence and advances in miniaturization and multiplexing were essential to the creation of techniques such as RNA-seq, ChIP-seq, and high-throughput behavioral and chemical screens. Multiplexing was also instrumental in the recent enhancement of the classic yeast one-hybrid inte...

journal_title：Zebrafish

authors： King J,Foster J,Davison JM,Rawls JF,Breton G

更新日期：2018-04-01 00:00:00

abstract：:Embryo surface disinfection is utilized in aquaculture to decrease the risk of pathogen introduction into established colonies. Zebrafish embryos are commonly disinfected with unbuffered sodium hypochlorite at 25-50 ppm for 10 min with or without concurrent treatment with chemicals, including pronase (Pron), sodium th...

journal_title：Zebrafish

authors： Peneyra SM,Lerpiriyapong K,Riedel ER,Lipman NS,Lieggi C

更新日期：2020-10-01 00:00:00

abstract：:By means of scientometric indicators, this study investigated the characteristics of scientific production and research collaboration involving zebrafish (Danio rerio) in Brazilian Science indexed by the Web of Science (WoS). Citation data were collected from the WoS and data regarding Impact Factor (IF) were gathered...

journal_title：Zebrafish

authors： Gheno EM,Rosemberg DB,Souza DO,Calabró L

更新日期：2016-06-01 00:00:00

abstract：:Understanding living systems requires an in-depth knowledge of the signaling networks that drive cellular homeostasis, regulate intercellular communication, and contribute to cell fates during development. Several resources exist to provide high-throughput data sets or manually curated interaction information from hum...

journal_title：Zebrafish

authors： Csályi K,Fazekas D,Kadlecsik T,Türei D,Gul L,Horváth B,Módos D,Demeter A,Pápai N,Lenti K,Csermely P,Vellai T,Korcsmáros T,Varga M

更新日期：2016-12-01 00:00:00

abstract：:Extracellular matrix (ECM) remodeling is a process that is crucial to the development of embryos, the growth and metastasis of tumors, and wound healing and homeostasis of tissues in adults. As such, it involves dozens of gene products that are regulated by mechanisms operating at transcriptional and multiple posttran...

journal_title：Zebrafish

authors： Wyatt RA,Keow JY,Harris ND,Haché CA,Li DH,Crawford BD

更新日期：2009-12-01 00:00:00

abstract：:The two LIM kinases (LIMKs) LIMK1 and LIMK2 are members of the PDZ/LIM family. These serine/threonine protein kinases are involved in actin cytoskeleton reorganization through phosphorylation and inactivation of ADF/cofilin. Different subcellular localizations of LIMK1 and LIMK2 suggest different functions. LIMK1 is i...

journal_title：Zebrafish

authors： Vlecken DH,Bagowski CP

更新日期：2009-12-01 00:00:00

abstract：:The use of antibiotics for anti-infection and growth promotion has caused the overuse of antibiotics in aquaculture. However, the benefit or risk of the long-term use of antibiotics on fish growth or health has not been fully addressed. In the present study, zebrafish were fed with sulfamethoxazole (SMX) or oxytetracy...

journal_title：Zebrafish

authors： Zhou L,Limbu SM,Qiao F,Du ZY,Zhang M

更新日期：2018-08-01 00:00:00

abstract：:Spring viremia of carp virus (SVCV) causes the skin hemorrhagic disease in cyprinid species, but its molecular mechanism of skin immune response remains unclear at the protein level. In the present study, the differential proteomics of the zebrafish (Danio rerio) skin in response to SVCV infection were examined by iso...

journal_title：Zebrafish

authors： Liu R,Hu X,Lü A,Song Y,Lian Z,Sun J,Sung YY

更新日期：2020-04-01 00:00:00

abstract：:Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DN...

journal_title：Zebrafish

authors： Kuriya K,Higashiyama E,Avşar-Ban E,Tamaru Y,Ogata S,Takebayashi S,Ogata M,Okumura K

更新日期：2015-12-01 00:00:00

abstract：:Dimethyl sulfoxide (DMSO), a widely used carrier solvent, can be toxic to test organisms and has species-specific sensitivity. In this study, the developmental toxicity and stress protein responses of DMSO to rare minnow (Gobiocypris rarus) and zebrafish (Danio rerio) with two tests were compared in the early life sta...

journal_title：Zebrafish

authors： Xiong X,Luo S,Wu B,Wang J

更新日期：2017-02-01 00:00:00

abstract：:Analysis of genes compromising the glomerular filtration barrier in rodent models using transgenic or knockdown approaches is time- and resource-consuming and often leads to unsatisfactory results. Therefore, it would be beneficial to have a selection tool indicating that your gene of interest is in fact associated wi...

journal_title：Zebrafish

authors： Hanke N,King BL,Vaske B,Haller H,Schiffer M

更新日期：2015-10-01 00:00:00

abstract：:After its introduction for scientific investigation in the 1950s, the cypriniform zebrafish, Danio rerio, has become a valuable model for the study of regenerative processes and mechanisms. Zebrafish exhibit epimorphic regeneration, in which a nondifferentiated cell mass formed after amputation is able to fully regene...

journal_title：Zebrafish

authors： Lebedeva L,Zhumabayeva B,Gebauer T,Kisselev I,Aitasheva Z

更新日期：2020-12-01 00:00:00

abstract：:Pamela Raymond received her B.S., M.S., and Ph.D. degrees from the University of Michigan. She is currently Professor of Molecular, Cellular, and Developmental Biology in the College of Literature, Science, and the Arts at the University of Michigan. She has held academic appointments at Harvard Medical School and the...

journal_title：Zebrafish

authors： Raymond P

更新日期：2005-01-01 00:00:00

abstract：:The European Zebrafish Principal Investigator Meeting (EZPM) is an ideal forum for group leaders using this fantastic animal model not only to discuss science but also to strengthen their interactions, to push forward technological advances, and to define guidelines for the use of this fish in research. The city of Li...

journal_title：Zebrafish

authors： Lopes SS,Distel M,Linker C,Fior R,Monteiro R,Bianco IH,Portugues R,Strähle U,Saúde L

更新日期：2016-12-01 00:00:00

abstract：:To promote application of the fruits of zebrafish research, we need to cultivate understanding of the use of zebrafish not only in the research community but also among the general public. On Open Days at our institute, the National Institute for Environmental Studies (NIES), our zebrafish group exhibits our research ...

journal_title：Zebrafish

authors： Aoki Y

更新日期：2009-06-01 00:00:00

abstract：:Dr. Manfred Schartl is Professor in the Department of Physiological Chemistry at the Biocenter of the University of Würzburg, Germany and Adjunct Professor for Experimental Cancer Research at the University of Bergen, Norway. He pursued undergraduate studies in biology and graduate work at the University of Giebetaen,...

journal_title：Zebrafish

authors： Schartl M

更新日期：2004-01-01 00:00:00

abstract：:Dr. Zhiyuan Gong is Associate Professor in the Department of Biological Sciences, National University of Singapore. He graduated with a B.Sc. degree in marine biology from Ocean University of China and received his Ph.D. degree in developmental biology from McGill University in Canada. He spent many years at the Unive...

journal_title：Zebrafish

authors： Gong Z

更新日期：2004-01-01 00:00:00

abstract：:Wild zebrafish exhibit a wide range of behavior. We found abundant wild zebrafish in flowing rivers and still water, in large, tightly-knit groups of hundreds of individuals, as well as in small, loose shoals. In two still-water populations, zebrafish were quite small in body size, common, and in tight groups of up to...

journal_title：Zebrafish

authors： Suriyampola PS,Shelton DS,Shukla R,Roy T,Bhat A,Martins EP

更新日期：2016-02-01 00:00:00