In Vivo Determination of Body Composition in Zebrafish (Danio rerio) by Quantitative Magnetic Resonance.

Abstract:

:Zebrafish (Danio rerio) as a model research organism continues to expand its relevance and role in multiple research disciplines, with recent work directed toward models of metabolism, nutrition, and energetics. Multiple technologies exist to assess body composition in animal research models at various levels of detail (tissues/organs, body regions, and whole organism). The development and/or validation of body composition assessment tools can open new areas of research questions for a given organism. Using fish from a comparative nutrition study, quantitative magnetic resonance (QMR) assessment of whole body fat and fat-free mass (FFM) in live fish was performed. QMR measures from two cohorts (n = 26 and n = 27) were compared with chemical carcass analysis (CCA) of FM and FFM. QMR was significantly correlated with chemical carcass values (fat, p < 0.001; lean, p = 0.002), although QMR significantly overestimated fat mass (FM) (0.011 g; p < 0.0001) and underestimated FFM (-0.024 g; p < 0.0001) relative to CCA. In a separate cross-validation group of fish, prediction equations corrected carcass values for FM (p = 0.121) and FFM (p = 0.753). These results support the utilization of QMR-a nonlethal nondestructive method-for cross-sectional or longitudinal body composition assessment outcomes in zebrafish.

journal_name

Zebrafish

journal_title

Zebrafish

authors

Fowler LA,Dennis LN,Barry RJ,Powell ML,Watts SA,Smith DL Jr

doi

10.1089/zeb.2015.1157

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

170-6

issue

3

eissn

1545-8547

issn

1557-8542

journal_volume

13

pub_type

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