Abstract:
:This project was developed to promote understanding of how mathematics and statistical analysis are used as tools in genetic research. It gives students the opportunity to carry out hypothesis-driven experiments in the classroom: students generate hypotheses about Mendelian and non-Mendelian inheritance patterns, gather raw data, and test their hypotheses using chi-square statistical analysis. In the first protocol, students are challenged to analyze inheritance patterns using GloFish, brightly colored, commercially available, transgenic zebrafish that express Green, Yellow, or Red Fluorescent Protein throughout their muscles. In the second protocol, students learn about genetic screens, microscopy, and developmental biology by analyzing the inheritance patterns of mutations that cause developmental defects. The difficulty of the experiments can be adapted for middle school to upper level undergraduate students. Since the GloFish experiments use only fish and materials that can be purchased from pet stores, they should be accessible to many schools. For each protocol, we provide detailed instructions, ideas for how the experiments fit into an undergraduate curriculum, raw data, and example analyses. Our plan is to have these protocols form the basis of a growing and adaptable educational tool available on the Zebrafish in the Classroom Web site.
journal_name
Zebrafishjournal_title
Zebrafishauthors
Lindemann S,Senkler J,Auchter E,Liang JOdoi
10.1089/zeb.2010.0686subject
Has Abstractpub_date
2011-06-01 00:00:00pages
41-55issue
2eissn
1545-8547issn
1557-8542journal_volume
8pub_type
杂志文章相关文献
Zebrafish文献大全abstract::The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacen...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2014.0999
更新日期:2015-02-01 00:00:00
abstract::Many facilities house fish in separate static containers post-procedure, for example, while awaiting genotyping results. This ensures fish can be easily identified, but it does not allow for provision of continuous filtered water or diet. At the Wellcome Trust Sanger Institute, concern over the housing conditions led ...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2015.1165
更新日期:2016-07-01 00:00:00
abstract::Doradidae has been a target of phylogenetic studies over the last few years, but chromosomal information about the family is still scarce. Therefore, new cytogenetic data are provided herein and they are correlated with phylogenetic proposals to contribute to the knowledge of chromosomal evolution within doradids. Cyt...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2017.1554
更新日期:2018-06-01 00:00:00
abstract::Microcystin-leucine arginine (MC-LR) is a natural toxin produced by cyanobacterial blooms that causes severe liver damage in fish. It is crucial to investigate if housekeeping genes are affected by MC-LR in zebrafish, to permit the adequate evaluation of gene expression by RT-qPCR. We evaluated the gene expression sta...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2018.1598
更新日期:2018-10-01 00:00:00
abstract::The Cannabis sativa plant contains numerous phytocannabinoids and terpenes with known or potential biological activity. For decades, plant breeders have been breeding the Cannabis plant to control for a desired ratio of the major cannabinoids. A high-throughput in vivo model to understand the relationship between the ...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2017.1541
更新日期:2018-08-01 00:00:00
abstract::The use of antibiotics for anti-infection and growth promotion has caused the overuse of antibiotics in aquaculture. However, the benefit or risk of the long-term use of antibiotics on fish growth or health has not been fully addressed. In the present study, zebrafish were fed with sulfamethoxazole (SMX) or oxytetracy...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2017.1526
更新日期:2018-08-01 00:00:00
abstract::After its introduction for scientific investigation in the 1950s, the cypriniform zebrafish, Danio rerio, has become a valuable model for the study of regenerative processes and mechanisms. Zebrafish exhibit epimorphic regeneration, in which a nondifferentiated cell mass formed after amputation is able to fully regene...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2020.1926
更新日期:2020-12-01 00:00:00
abstract::Wild zebrafish exhibit a wide range of behavior. We found abundant wild zebrafish in flowing rivers and still water, in large, tightly-knit groups of hundreds of individuals, as well as in small, loose shoals. In two still-water populations, zebrafish were quite small in body size, common, and in tight groups of up to...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2015.1159
更新日期:2016-02-01 00:00:00
abstract::Embryo surface disinfection is utilized in aquaculture to decrease the risk of pathogen introduction into established colonies. Zebrafish embryos are commonly disinfected with unbuffered sodium hypochlorite at 25-50 ppm for 10 min with or without concurrent treatment with chemicals, including pronase (Pron), sodium th...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2020.1917
更新日期:2020-10-01 00:00:00
abstract::Electronic databases provide effective and efficient management of zebrafish colony operations, but commercially available options are expensive. In this study we have developed a free zebrafish management repository alternative using free Google applications. Husbandry information is logged into a Google Sheets-based...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2018.1583
更新日期:2018-12-01 00:00:00
abstract::One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) pr...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2005.2.105
更新日期:2005-01-01 00:00:00
abstract::Understanding living systems requires an in-depth knowledge of the signaling networks that drive cellular homeostasis, regulate intercellular communication, and contribute to cell fates during development. Several resources exist to provide high-throughput data sets or manually curated interaction information from hum...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2016.1277
更新日期:2016-12-01 00:00:00
abstract::The study of myocardial transmembrane ion currents is fundamental to understand frequent pathologies such as arrhythmias and ischemia. Conventional electrocardiography (ECG) is not able to record ion currents, while the use of intracellular microelectrodes in a beating heart has technical limitations. Myocardial monop...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2019.1753
更新日期:2019-10-01 00:00:00
abstract::Pamela Raymond received her B.S., M.S., and Ph.D. degrees from the University of Michigan. She is currently Professor of Molecular, Cellular, and Developmental Biology in the College of Literature, Science, and the Arts at the University of Michigan. She has held academic appointments at Harvard Medical School and the...
journal_title:Zebrafish
pub_type: 面试
doi:10.1089/zeb.2005.2.157
更新日期:2005-01-01 00:00:00
abstract::Mycobacteriosis is a common bacterial infection in laboratory zebrafish caused by several different species and strains of Mycobacterium, including both rapid and slow growers. One control measure used to prevent mycobacterial spread within and between facilities is surface disinfection of eggs. Recent studies have hi...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2015.1229
更新日期:2016-07-01 00:00:00
abstract::Zebrafish (Danio rerio) as a model research organism continues to expand its relevance and role in multiple research disciplines, with recent work directed toward models of metabolism, nutrition, and energetics. Multiple technologies exist to assess body composition in animal research models at various levels of detai...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2015.1157
更新日期:2016-06-01 00:00:00
abstract::A study was conducted to preliminarily assess the contribution of the intestinal microflora to biotin supply in zebrafish. Biotin and avidin were added to three isonitrogenous and isocaloric purified diets to provide molar avidin: biotin ratios of 0:0 (basal diet), 0:1 (biotin-supplemented diet), and 120:0. Another di...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2011.0706
更新日期:2011-12-01 00:00:00
abstract::Managing the welfare of laboratory animals is critical to animal health, vital in the understanding of phenotypes created by treatment or genetic alteration and ensures compliance of regulations. Part of an animal welfare assessment is the requirement to record observations, ensuring all those responsible for the anim...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2016.1248
更新日期:2016-07-01 00:00:00
abstract::Intestinal motility, the spontaneous and rhythmic smooth muscle contraction, is a complex process that is regulated by overlapping and redundant regulatory mechanisms. Primary regulators intrinsic to the gastrointestinal tract include interstitial cells of Cajal, enteric neurons, and smooth muscle cells. Extrinsic pri...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2017.1440
更新日期:2017-10-01 00:00:00
abstract::Dr. Zhiyuan Gong is Associate Professor in the Department of Biological Sciences, National University of Singapore. He graduated with a B.Sc. degree in marine biology from Ocean University of China and received his Ph.D. degree in developmental biology from McGill University in Canada. He spent many years at the Unive...
journal_title:Zebrafish
pub_type: 面试
doi:10.1089/zeb.2004.1.185
更新日期:2004-01-01 00:00:00
abstract::β-Cells of the islet of Langerhans produce insulin to maintain glucose homeostasis. Self-replication of β-cells is the predominant mode of postnatal β-cell production in mammals, with about 20% of rodent β cells dividing in a 24-hour period. However, replicating β-cells are rare in adults. Induction of self-replicatio...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2012.0816
更新日期:2013-06-01 00:00:00
abstract::Most young researchers leaving the safe haven of postdoctoral life for the unchartered territory of a PI position are confronted with a multitude of new tasks. For those using fish as their favorite model system, this often includes the daunting task of setting up and running a fish facility. New PIs are expected to k...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2012.0768
更新日期:2012-09-01 00:00:00
abstract::We are entering a new era in our ability to modify and edit the genomes of model organisms. Zinc finger nucleases (ZFNs) opened the door to the first custom nuclease-targeted genome engineering in the late 1990s. However, ZFNs remained out of reach for most research labs because of the difficulty of production, high c...
journal_title:Zebrafish
pub_type: 杂志文章,评审
doi:10.1089/zeb.2013.9999
更新日期:2013-03-01 00:00:00
abstract::A review of some exciting new directions for Xiphophorus research in behavioral ecology and integrative animal behavior is presented. The primary objective of this review is to identify areas of research in this teleost genus that, if studied further, could yield insights into the manifold causes and consequences of d...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2006.3.287
更新日期:2006-01-01 00:00:00
abstract::Individual differences (IDs) in behavior among nonhuman animals have been documented in a wide range of taxa. Although traditionally considered noise around an average, other potentially adaptive sources of phenotypic variation exist. IDs in behavior that are consistent across time and context are more recently recogn...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2013.0952
更新日期:2014-08-01 00:00:00
abstract::Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this st...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2012.0770
更新日期:2012-09-01 00:00:00
abstract::Extracellular matrix (ECM) remodeling is a process that is crucial to the development of embryos, the growth and metastasis of tumors, and wound healing and homeostasis of tissues in adults. As such, it involves dozens of gene products that are regulated by mechanisms operating at transcriptional and multiple posttran...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2009.0609
更新日期:2009-12-01 00:00:00
abstract::By means of scientometric indicators, this study investigated the characteristics of scientific production and research collaboration involving zebrafish (Danio rerio) in Brazilian Science indexed by the Web of Science (WoS). Citation data were collected from the WoS and data regarding Impact Factor (IF) were gathered...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2015.1183
更新日期:2016-06-01 00:00:00
abstract::Studies on parental aging are a very attractive field, although it is poorly understood how parental age affects embryonic development and adult traits of the offspring. In this study, we used the turquoise killifish Nothobranchius furzeri, as is the vertebrate with shortest captive lifespan and an interesting model. ...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2017.1494
更新日期:2018-04-01 00:00:00
abstract::Traditional working heart preparations, based on the original Langendorff setup, are widely used experimental models that have tremendously advanced the cardiovascular field. However, these systems can be deceivingly complex, requiring the maintenance of pH with CO(2), the delivery of oxygenated perfusate, and the nee...
journal_title:Zebrafish
pub_type: 杂志文章
doi:10.1089/zeb.2008.0550
更新日期:2008-12-01 00:00:00