当前位置： SCI文献检索 > Cell Reports期刊下所有文献 > Growth of B Cell Receptor Microclusters Is Regulated by PIP2 and PIP3 Equilibrium and Dock2 Recruitment and Activation.

Growth of B Cell Receptor Microclusters Is Regulated by PIP2 and PIP3 Equilibrium and Dock2 Recruitment and Activation.

Abstract：

:The growth of B cell receptor (BCR) microclusters upon antigen stimulation drives B cell activation. Here, we show that PI3K-mediated PIP3 production is required for the growth of BCR microclusters. This growth is likely inhibited by PTEN and dependent on its plasma membrane binding and lipid phosphatase activities. Mechanistically, we find that PIP3-dependent recruitment and activation of a guanine nucleotide exchange factor, Dock2, is required for the sustained growth of BCR microclusters through remodeling of the F-actin cytoskeleton. As a consequence, Dock2 deficiency significantly disrupts the structure of the B cell immunological synapse. Finally, we find that primary B cells from systemic lupus erythematosus (SLE) patients exhibit more prominent BCR and PI3K microclusters than B cells from healthy controls. These results demonstrate the importance of a PI3K- and PTEN-governed PIP2 and PIP3 equilibrium in regulating the activation of B cells through Dock2-controlled growth of BCR microclusters.

journal_name

Cell Rep

journal_title

Cell reports

authors

Wang J,Xu L,Shaheen S,Liu S,Zheng W,Sun X,Li Z,Liu W

doi

10.1016/j.celrep.2017.10.117

subject

Has Abstract

pub_date

2017-11-28 00:00:00

pages

2541-2557

issue

9

issn

2211-1247

pii

S2211-1247(17)31597-8

journal_volume

21

pub_type

杂志文章

相关文献

Cell Reports文献大全
  • Fcμ receptor as a Costimulatory Molecule for T Cells.

    abstract：:Fc receptor for IgM (FcμR)-deficient mice display dysregulated function of neutrophils, dendritic cells, and B cells. The relevance of FcμR to human T cells is still unknown. We show that FcμR is mostly stored inside the cell and that surface expression is tightly regulated. Decreased surface expression on T cells fro...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.02.024

    authors： Meryk A,Pangrazzi L,Hagen M,Hatzmann F,Jenewein B,Jakic B,Hermann-Kleiter N,Baier G,Jylhävä J,Hurme M,Trieb K,Grubeck-Loebenstein B

    更新日期：2019-03-05 00:00:00

  • Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom.

    abstract：:PIWI-interacting RNAs (piRNAs) protect the genome from transposons in animal gonads. Maelstrom (Mael) is an evolutionarily conserved protein, composed of a high-mobility group (HMG) domain and a MAEL domain, and is essential for piRNA-mediated transcriptional transposon silencing in various species, such as Drosophila...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.03.030

    authors： Matsumoto N,Sato K,Nishimasu H,Namba Y,Miyakubi K,Dohmae N,Ishitani R,Siomi H,Siomi MC,Nureki O

    更新日期：2015-04-21 00:00:00

  • Poly(A)-specific ribonuclease mediates 3'-end trimming of Argonaute2-cleaved precursor microRNAs.

    abstract：:MicroRNAs (miRNAs) are typically generated as ~22-nucleotide double-stranded RNAs via the processing of precursor hairpins by the ribonuclease III enzyme Dicer, after which they are loaded into Argonaute (Ago) proteins to form an RNA-induced silencing complex (RISC). However, the biogenesis of miR-451, an erythropoiet...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2013.09.029

    authors： Yoda M,Cifuentes D,Izumi N,Sakaguchi Y,Suzuki T,Giraldez AJ,Tomari Y

    更新日期：2013-11-14 00:00:00

  • Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons.

    abstract：:Ribosomes and a subset of cellular mRNAs are trafficked into axons of developing neurons. The axonal localization of translational machinery allows new proteins to be rapidly and locally synthesized during axonal growth and pathfinding. However, in mature neurons, axonal ribosomes are significantly reduced or even abs...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.06.080

    authors： Costa RO,Martins H,Martins LF,Cwetsch AW,Mele M,Pedro JR,Tomé D,Jeon NL,Cancedda L,Jaffrey SR,Almeida RD

    更新日期：2019-07-23 00:00:00

  • Oxysterol-Binding Protein-Related Protein 1L Regulates Cholesterol Egress from the Endo-Lysosomal System.

    abstract：:Lipoprotein cholesterol is delivered to the limiting membrane of late endosomes/lysosomes (LELs) by Niemann-Pick C1 (NPC1). However, the mechanism of cholesterol transport from LELs to the endoplasmic reticulum (ER) is poorly characterized. We report that oxysterol-binding protein-related protein 1L (ORP1L) is necessa...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.05.028

    authors： Zhao K,Ridgway ND

    更新日期：2017-05-30 00:00:00

  • Phospho-Ser784-VCP Is Required for DNA Damage Response and Is Associated with Poor Prognosis of Chemotherapy-Treated Breast Cancer.

    abstract：:Spatiotemporal protein reorganization at DNA damage sites induced by genotoxic chemotherapies is crucial for DNA damage response (DDR), which influences treatment response by directing cancer cell fate. This process is orchestrated by valosin-containing protein (VCP), an AAA+ ATPase that extracts polyubiquinated chrom...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.107745

    authors： Zhu C,Rogers A,Asleh K,Won J,Gao D,Leung S,Li S,Vij KR,Zhu J,Held JM,You Z,Nielsen TO,Shao J

    更新日期：2020-06-09 00:00:00

  • Protein Expression Landscape of Mouse Embryos during Pre-implantation Development.

    abstract：:Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of the genetic programs underlying this proce...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.11.111

    authors： Gao Y,Liu X,Tang B,Li C,Kou Z,Li L,Liu W,Wu Y,Kou X,Li J,Zhao Y,Yin J,Wang H,Chen S,Liao L,Gao S

    更新日期：2017-12-26 00:00:00

  • Adaptive Evolution of MERS-CoV to Species Variation in DPP4.

    abstract：:Middle East Respiratory Syndrome Coronavirus (MERS-CoV) likely originated in bats and passed to humans through dromedary camels; however, the genetic mechanisms underlying cross-species adaptation remain poorly understood. Variation in the host receptor, dipeptidyl peptidase 4 (DPP4), can block the interaction with th...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.07.045

    authors： Letko M,Miazgowicz K,McMinn R,Seifert SN,Sola I,Enjuanes L,Carmody A,van Doremalen N,Munster V

    更新日期：2018-08-14 00:00:00

  • Metabolic Control of Gametophore Shoot Formation through Arginine in the Moss Physcomitrium patens.

    abstract：:Shoot formation is accompanied by active cell proliferation and expansion, requiring that metabolic state adapts to developmental control. Despite the importance of such metabolic reprogramming, it remains unclear how development and metabolism are integrated. Here, we show that disruption of ANGUSTIFOLIA3 orthologs (...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.108127

    authors： Kawade K,Horiguchi G,Hirose Y,Oikawa A,Hirai MY,Saito K,Fujita T,Tsukaya H

    更新日期：2020-09-08 00:00:00

  • Structural Insights into the Unique Activation Mechanisms of a Non-classical Calpain and Its Disease-Causing Variants.

    abstract：:Increased calpain activity is linked to neuroinflammation including a heritable retinal disease caused by hyper-activating mutations in the calcium-activated calpain-5 (CAPN5) protease. Although structures for classical calpains are known, the structure of CAPN5, a non-classical calpain, remains undetermined. Here we ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.12.077

    authors： Velez G,Sun YJ,Khan S,Yang J,Herrmann J,Chemudupati T,MacLaren RE,Gakhar L,Wakatsuki S,Bassuk AG,Mahajan VB

    更新日期：2020-01-21 00:00:00

  • Stratum, a Homolog of the Human GEF Mss4, Partnered with Rab8, Controls the Basal Restriction of Basement Membrane Proteins in Epithelial Cells.

    abstract：:The basement membrane (BM), a sheet of extracellular matrix lining the basal side of epithelia, is essential for epithelial cell function and integrity, yet the mechanisms that control the basal restriction of BM proteins are poorly understood. In epithelial cells, a specialized pathway is dedicated to restrict the de...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.02.002

    authors： Devergne O,Sun GH,Schüpbach T

    更新日期：2017-02-21 00:00:00

  • Agrin as a Mechanotransduction Signal Regulating YAP through the Hippo Pathway.

    abstract：:The Hippo pathway effectors YAP and TAZ act as nuclear sensors of mechanical signals in response to extracellular matrix (ECM) cues. However, the identity and nature of regulators in the ECM and the precise pathways relaying mechanoresponsive signals into intracellular sensors remain unclear. Here, we uncover a functi...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.02.041

    authors： Chakraborty S,Njah K,Pobbati AV,Lim YB,Raju A,Lakshmanan M,Tergaonkar V,Lim CT,Hong W

    更新日期：2017-03-07 00:00:00

  • A targeted oligonucleotide enhancer of SMN2 exon 7 splicing forms competing quadruplex and protein complexes in functional conditions.

    abstract：:The use of oligonucleotides to activate the splicing of selected exons is limited by a poor understanding of the mechanisms affected. A targeted bifunctional oligonucleotide enhancer of splicing (TOES) anneals to SMN2 exon 7 and carries an exonic splicing enhancer (ESE) sequence. We show that it stimulates splicing sp...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2014.08.051

    authors： Smith LD,Dickinson RL,Lucas CM,Cousins A,Malygin AA,Weldon C,Perrett AJ,Bottrill AR,Searle MS,Burley GA,Eperon IC

    更新日期：2014-10-09 00:00:00

  • Timed Regulation of 3BP2 Induction Is Critical for Sustaining CD8+ T Cell Expansion and Differentiation.

    abstract：:Successful anti-viral response requires the sustained activation and expansion of CD8+ T cells for periods that far exceed the time limit of physical T cell interaction with antigen-presenting cells (APCs). The expanding CD8+ T cell pool generates the effector and memory cell populations that provide viral clearance a...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.06.075

    authors： Dimitriou ID,Lee K,Akpan I,Lind EF,Barr VA,Ohashi PS,Samelson LE,Rottapel R

    更新日期：2018-07-31 00:00:00

  • Nuclear Localization of Huntingtin mRNA Is Specific to Cells of Neuronal Origin.

    abstract：:Huntington's disease (HD) is a monogenic neurodegenerative disorder representing an ideal candidate for gene silencing with oligonucleotide therapeutics (i.e., antisense oligonucleotides [ASOs] and small interfering RNAs [siRNAs]). Using an ultra-sensitive branched fluorescence in situ hybridization (FISH) method, we ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.07.106

    authors： Didiot MC,Ferguson CM,Ly S,Coles AH,Smith AO,Bicknell AA,Hall LM,Sapp E,Echeverria D,Pai AA,DiFiglia M,Moore MJ,Hayward LJ,Aronin N,Khvorova A

    更新日期：2018-09-04 00:00:00

  • CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites.

    abstract：:The role of CTCF in stabilizing long-range interactions between chromatin sites essential for maintaining nuclear architecture is well established. Most of these interactions involve recruitment of the cohesin complex to chromatin via CTCF. We find that CTCF also interacts with the centromeric protein CENP-E both in v...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.08.005

    authors： Xiao T,Wongtrakoongate P,Trainor C,Felsenfeld G

    更新日期：2015-09-08 00:00:00

  • Sumoylation of Smc5 Promotes Error-free Bypass at Damaged Replication Forks.

    abstract：:Replication of a damaged DNA template can threaten the integrity of the genome, requiring the use of various mechanisms to tolerate DNA lesions. The Smc5/6 complex, together with the Nse2/Mms21 SUMO ligase, plays essential roles in genome stability through undefined tasks at damaged replication forks. Various subunits...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.10.123

    authors： Zapatka M,Pociño-Merino I,Heluani-Gahete H,Bermúdez-López M,Tarrés M,Ibars E,Solé-Soler R,Gutiérrez-Escribano P,Apostolova S,Casas C,Aragon L,Wellinger R,Colomina N,Torres-Rosell J

    更新日期：2019-12-03 00:00:00

  • Fusion of Reprogramming Factors Alters the Trajectory of Somatic Lineage Conversion.

    abstract：:Simultaneous expression of Oct4, Klf4, Sox2, and cMyc induces pluripotency in somatic cells (iPSCs). Replacing Oct4 with the neuro-specific factor Brn4 leads to transdifferentiation of fibroblasts into induced neural stem cells (iNSCs). However, Brn4 was recently found to induce transient acquisition of pluripotency b...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.03.023

    authors： Velychko S,Kang K,Kim SM,Kwak TH,Kim KP,Park C,Hong K,Chung C,Hyun JK,MacCarthy CM,Wu G,Schöler HR,Han DW

    更新日期：2019-04-02 00:00:00

  • PTPMT1 Inhibition Lowers Glucose through Succinate Dehydrogenase Phosphorylation.

    abstract：:Virtually all organisms seek to maximize fitness by matching fuel availability with energy expenditure. In vertebrates, glucose homeostasis is central to this process, with glucose levels finely tuned to match changing energy requirements. To discover new pathways regulating glucose levels in vivo, we performed a larg...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.01.010

    authors： Nath AK,Ryu JH,Jin YN,Roberts LD,Dejam A,Gerszten RE,Peterson RT

    更新日期：2015-02-10 00:00:00

  • The Yeast Prion [SWI(+)] Abolishes Multicellular Growth by Triggering Conformational Changes of Multiple Regulators Required for Flocculin Gene Expression.

    abstract：:Although transcription factors are prevalent among yeast prion proteins, the role of prion-mediated transcriptional regulation remains elusive. Here, we show that the yeast prion [SWI(+)] abolishes flocculin (FLO) gene expression and results in a complete loss of multicellularity. Further investigation demonstrates th...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.11.060

    authors： Du Z,Zhang Y,Li L

    更新日期：2015-12-29 00:00:00

  • Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development.

    abstract：:We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated, short-duration and long-duration spaceflight. Previous studies have identified miRNAs that regulate rodent responses to spaceflight in low-Earth orbit, and we have confirm...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.108448

    authors： Malkani S,Chin CR,Cekanaviciute E,Mortreux M,Okinula H,Tarbier M,Schreurs AS,Shirazi-Fard Y,Tahimic CGT,Rodriguez DN,Sexton BS,Butler D,Verma A,Bezdan D,Durmaz C,MacKay M,Melnick A,Meydan C,Li S,Garrett-Bakelman F,

    更新日期：2020-12-08 00:00:00

  • High-Resolution Mapping of RNA Polymerases Identifies Mechanisms of Sensitivity and Resistance to BET Inhibitors in t(8;21) AML.

    abstract：:Bromodomain and extra-terminal domain (BET) family inhibitors offer an approach to treating hematological malignancies. We used precision nuclear run-on transcription sequencing (PRO-seq) to create high-resolution maps of active RNA polymerases across the genome in t(8;21) acute myeloid leukemia (AML), as these polyme...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2016.07.032

    authors： Zhao Y,Liu Q,Acharya P,Stengel KR,Sheng Q,Zhou X,Kwak H,Fischer MA,Bradner JE,Strickland SA,Mohan SR,Savona MR,Venters BJ,Zhou MM,Lis JT,Hiebert SW

    更新日期：2016-08-16 00:00:00

  • Alterations in Phosphorylation of Hepatocyte Ribosomal Protein S6 Control Plasmodium Liver Stage Infection.

    abstract：:Plasmodium parasites are highly selective when infecting hepatocytes and induce many changes within the host cell upon infection. While several host cell factors have been identified that are important for liver infection, our understanding of what facilitates the maintenance of infection remains incomplete. Here, we ...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2019.02.085

    authors： Glennon EKK,Austin LS,Arang N,Kain HS,Mast FD,Vijayan K,Aitchison JD,Kappe SHI,Kaushansky A

    更新日期：2019-03-19 00:00:00

  • Sleep Induction by Mechanosensory Stimulation in Drosophila.

    abstract：:People tend to fall asleep when gently rocked or vibrated. Experimental studies have shown that rocking promotes sleep in humans and mice. However, the mechanisms underlying the phenomenon are not well understood. A habituation model proposes that habituation, a form of non-associative learning, mediates sleep inducti...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.108462

    authors： Öztürk-Çolak A,Inami S,Buchler JR,McClanahan PD,Cruz A,Fang-Yen C,Koh K

    更新日期：2020-12-01 00:00:00

  • Chromatin Modifiers SET-25 and SET-32 Are Required for Establishment but Not Long-Term Maintenance of Transgenerational Epigenetic Inheritance.

    abstract：:Some epigenetic modifications are inherited from one generation to the next, providing a potential mechanism for the inheritance of environmentally acquired traits. Transgenerational inheritance of RNAi phenotypes in Caenorhabditis elegans provides an excellent model to study this phenomenon, and although studies have...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2018.10.085

    authors： Woodhouse RM,Buchmann G,Hoe M,Harney DJ,Low JKK,Larance M,Boag PR,Ashe A

    更新日期：2018-11-20 00:00:00

  • Antibiotics Disrupt Coordination between Transcriptional and Phenotypic Stress Responses in Pathogenic Bacteria.

    abstract：:Bacterial genes that change in expression upon environmental disturbance have commonly been seen as those that must also phenotypically matter. However, several studies suggest that differentially expressed genes are rarely phenotypically important. We demonstrate, for Gram-positive and Gram-negative bacteria, that th...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2017.07.062

    authors： Jensen PA,Zhu Z,van Opijnen T

    更新日期：2017-08-15 00:00:00

  • Suppression of PGC-1α Is Critical for Reprogramming Oxidative Metabolism in Renal Cell Carcinoma.

    abstract：:Long believed to be a byproduct of malignant transformation, reprogramming of cellular metabolism is now recognized as a driving force in tumorigenesis. In clear cell renal cell carcinoma (ccRCC), frequent activation of HIF signaling induces a metabolic switch that promotes tumorigenesis. Here, we demonstrate that PGC...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2015.06.006

    authors： LaGory EL,Wu C,Taniguchi CM,Ding CC,Chi JT,von Eyben R,Scott DA,Richardson AD,Giaccia AJ

    更新日期：2015-07-07 00:00:00

  • A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level.

    abstract：:High-throughput single-cell RNA sequencing (scRNA-seq) has become a frequently used tool to assess immune cell heterogeneity. Recently, the combined measurement of RNA and protein expression was developed, commonly known as cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq). Acquisition of prote...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.03.063

    authors： Mair F,Erickson JR,Voillet V,Simoni Y,Bi T,Tyznik AJ,Martin J,Gottardo R,Newell EW,Prlic M

    更新日期：2020-04-07 00:00:00

  • SIX2 Regulates Human β Cell Differentiation from Stem Cells and Functional Maturation In Vitro.

    abstract：:Generation of insulin-secreting β cells in vitro is a promising approach for diabetes cell therapy. Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are differentiated to β cells (SC-β cells) and mature to undergo glucose-stimulated insulin secretion, but molecular regulation of thi...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.107687

    authors： Velazco-Cruz L,Goedegebuure MM,Maxwell KG,Augsornworawat P,Hogrebe NJ,Millman JR

    更新日期：2020-05-26 00:00:00

  • Kainate Receptor Activation Shapes Short-Term Synaptic Plasticity by Controlling Receptor Lateral Mobility at Glutamatergic Synapses.

    abstract：:Kainate receptors (KARs) mediate postsynaptic currents with a key impact on neuronal excitability. However, the molecular determinants controlling KAR postsynaptic localization and stabilization are poorly understood. Here, we exploit optogenetic and single-particle tracking approaches to study the role of KAR conform...

    journal_title：Cell reports

    pub_type： 杂志文章

    doi：10.1016/j.celrep.2020.107735

    authors： Polenghi A,Nieus T,Guazzi S,Gorostiza P,Petrini EM,Barberis A

    更新日期：2020-06-09 00:00:00