Abstract：
:Middle East Respiratory Syndrome Coronavirus (MERS-CoV) likely originated in bats and passed to humans through dromedary camels; however, the genetic mechanisms underlying cross-species adaptation remain poorly understood. Variation in the host receptor, dipeptidyl peptidase 4 (DPP4), can block the interaction with the MERS-CoV spike protein and form a species barrier to infection. To better understand the species adaptability of MERS-CoV, we identified a suboptimal species-derived variant of DPP4 to study viral adaption. Passaging virus on cells expressing this DPP4 variant led to accumulation of mutations in the viral spike which increased replication. Parallel passages revealed distinct paths of viral adaptation to the same DPP4 variant. Structural analysis and functional assays showed that these mutations enhanced viral entry with suboptimal DPP4 by altering the surface charge of spike. These findings demonstrate that MERS-CoV spike can utilize multiple paths to rapidly adapt to novel species variation in DPP4.
journal_name
Cell Repjournal_title
Cell reportsauthors
Letko M,Miazgowicz K,McMinn R,Seifert SN,Sola I,Enjuanes L,Carmody A,van Doremalen N,Munster Vdoi
10.1016/j.celrep.2018.07.045subject
Has Abstractpub_date
2018-08-14 00:00:00pages
1730-1737issue
7issn
2211-1247pii
S2211-1247(18)31148-3journal_volume
24pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:The corpus callosum is the major axon tract that connects and integrates neural activity between the two cerebral hemispheres. Although ∼1:4,000 children are born with developmental absence of the corpus callosum, the primary etiology of this condition remains unknown. Here, we demonstrate that midline crossing of cal...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.09.033
更新日期：2016-10-11 00:00:00
abstract：:Experience-dependent synapse refinement is essential for functional optimization of neural circuits. However, how sensory experience sculpts excitatory synaptic transmission is poorly understood. Here, we show that despite substantial remodeling of synaptic connectivity, AMPAR-mediated synaptic transmission remains at...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.12.084
更新日期：2017-01-24 00:00:00
abstract：:Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates RB and functions as a collaborative nuclear oncogene. The serine threonine kinase Akt plays a pivotal role in the control of cellular metabolism, survival, and mitogenic signaling. Herein, Akt1-mediated phosphorylation of downstream substrate...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108151
更新日期：2020-09-15 00:00:00
abstract：:A two-step, automated, high-throughput RNAi silencing screen was used to identify host cell factors required during vaccinia virus infection. Validation and analysis of clustered hits revealed previously unknown processes during virus entry, including a mechanism for genome uncoating. Viral core proteins were found to...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2012.09.003
更新日期：2012-10-25 00:00:00
abstract：:The endothelial monolayer forms a barrier between the lumen of blood vessels and the underlying tissues. Stable VE-cadherin-based adherens junctions are essential for maintaining this barrier, whereas their remodeling is required for angiogenesis in health and disease. Here, we position the ERAD-associated ubiquitin l...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107944
更新日期：2020-08-04 00:00:00
abstract：:Cognitive dysfunction and decreased mobility from aging and neurodegenerative conditions, such as Parkinson and Alzheimer diseases, are major biomedical challenges in need of more effective therapies. Increasing brain resilience may represent a new treatment strategy. Klotho, a longevity factor, enhances cognition whe...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.07.024
更新日期：2017-08-08 00:00:00
abstract：:Although vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are under development, the antigen epitopes on the virus and their immunogenicity are poorly understood. Here, we simulate the 3D structures and predict the B cell epitopes on the spike (S), envelope (E), membrane (M), and nucleocap...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108666
更新日期：2021-01-26 00:00:00
abstract：:Immune cells can metabolize glucose, amino acids, and fatty acids (FAs) to generate energy. The roles of different FA species and their impacts on humoral immunity remain poorly understood. Here, we report that proliferating B cells require monounsaturated FAs (MUFAs) to maintain mitochondrial metabolism and mTOR acti...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108601
更新日期：2021-01-05 00:00:00
abstract：:Physicians have observed that surgical wounds in the elderly heal with thinner scars than wounds in young patients. Understanding this phenomenon may reveal strategies for promoting scarless wound repair. We show that full-thickness skin wounds in aged but not young mice fully regenerate. Exposure of aged animals to b...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.08.054
更新日期：2018-09-25 00:00:00
abstract：:Inhibitory receptors (IRs) function as critical regulators of immune responses by tempering T cell activity. In humans, several persisting viruses as well as cancers exploit IR signaling by upregulating IR ligands, resulting in suppression of T cell function (i.e., exhaustion). This allows escape from immune surveilla...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.10.119
更新日期：2019-12-03 00:00:00
abstract：:Successful anti-viral response requires the sustained activation and expansion of CD8+ T cells for periods that far exceed the time limit of physical T cell interaction with antigen-presenting cells (APCs). The expanding CD8+ T cell pool generates the effector and memory cell populations that provide viral clearance a...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.06.075
更新日期：2018-07-31 00:00:00
abstract：:Metazoan genomes are partitioned into modular chromosomal domains containing active or repressive chromatin. In flies, Polycomb group (PcG) response elements (PREs) recruit PHO and other DNA-binding factors and act as nucleation sites for the formation of Polycomb repressive domains. The sequence specificity of PREs i...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.08.072
更新日期：2014-10-09 00:00:00
abstract：:Embryonic stem cells (ESCs) exhibit high levels of ribosomal RNA (rRNA) transcription and ribosome biogenesis. Here, we reveal an unexpected role for an essential DEAD-box helicase, DDX18, in antagonizing the polycomb repressive complex 2 (PRC2) to prevent deposition of the repressive H3K27me3 mark onto rDNA in plurip...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.12.021
更新日期：2020-01-07 00:00:00
abstract：:Current theories of muscle contraction propose that the power stroke of a myosin motor is the sole source of mechanical energy driving the sliding filaments of a contracting muscle. These models exclude titin, the largest protein in the human body, which determines the passive elasticity of muscles. Here, we show that...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.01.025
更新日期：2016-02-16 00:00:00
abstract：:Maintaining genome integrity requires the accurate and complete replication of chromosomal DNA. This is of the utmost importance for embryonic stem cells (ESCs), which differentiate into cells of all lineages, including germ cells. However, endogenous and exogenous factors frequently induce stalling of replication for...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.01.014
更新日期：2014-02-27 00:00:00
abstract：:Glioblastoma (GBM) harbors subpopulations of therapy-resistant tumor-initiating cells (TICs) that are self-renewing and multipotent. To understand the regulation of the TIC state, we performed an image-based screen for genes regulating GBM TIC maintenance and identified ZFHX4, a 397 kDa transcription factor. ZFHX4 is ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.12.032
更新日期：2014-01-30 00:00:00
abstract：:Neurons exhibit dynamic structural changes in response to extracellular stimuli. Microtubules (MTs) provide rapid and dramatic cytoskeletal changes within the structural framework. However, the molecular mechanisms and signaling networks underlying MT dynamics remain unknown. Here, we have applied a comprehensive and ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.08.018
更新日期：2015-09-22 00:00:00
abstract：:The hair follicle (HF) is a complex miniorgan that serves as an ideal model system to study stem cell (SC) interactions with the niche during growth and regeneration. Dermal papilla (DP) cells are required for SC activation during the adult hair cycle, but signal exchange between niche and SC precursors/transit-amplif...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.02.078
更新日期：2016-03-29 00:00:00
abstract：:Bone morphogenic proteins (BMPs) are members of the transforming growth factor β (TGF-β) cytokine family promoting differentiation, homeostasis, and self-renewal of multiple tissues. We show that signaling through the bone morphogenic protein receptor 1α (BMPR1α) sustains expression of FOXP3 in Treg cells in periphera...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108219
更新日期：2020-10-06 00:00:00
abstract：:Primary afferents transduce environmental stimuli into electrical activity that is transmitted centrally to be decoded into corresponding sensations. However, it remains unknown how afferent populations encode different somatosensory inputs. To address this, we performed two-photon Ca2+ imaging from thousands of dorsa...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.04.065
更新日期：2018-05-15 00:00:00
abstract：:Color is continuous, yet we group colors into discrete categories associated with color names (e.g., yellow, blue). Color categorization is a case in point in the debate on how language shapes human cognition. Evidence suggests that color categorization depends on top-down input from the language system to the visual ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.08.003
更新日期：2019-09-03 00:00:00
abstract：:Simultaneous expression of Oct4, Klf4, Sox2, and cMyc induces pluripotency in somatic cells (iPSCs). Replacing Oct4 with the neuro-specific factor Brn4 leads to transdifferentiation of fibroblasts into induced neural stem cells (iNSCs). However, Brn4 was recently found to induce transient acquisition of pluripotency b...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.03.023
更新日期：2019-04-02 00:00:00
abstract：:Brain function is compromised in myotonic dystrophy type 1 (DM1), but the underlying mechanisms are not fully understood. To gain insight into the cellular and molecular pathways primarily affected, we studied a mouse model of DM1 and brains of adult patients. We found pronounced RNA toxicity in the Bergmann glia of t...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.06.006
更新日期：2017-06-27 00:00:00
abstract：:Working memory is mediated by the coordinated activation of frontal and parietal cortices occurring in the theta and alpha frequency ranges. Here, we test whether electrically stimulating frontal and parietal regions at the frequency of interaction is effective in modulating working memory. We identify working memory ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.10.072
更新日期：2019-11-26 00:00:00
abstract：:Hypoxia-inducible factors (HIFs) are crucial for cellular and organismal adaptation to hypoxia. The mitochondrial respiratory chain is the largest consumer of oxygen in most mammalian cells; however, it is unknown whether the respiratory chain is necessary for in vivo activation of HIFs and organismal adaptation to hy...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.03.044
更新日期：2016-04-19 00:00:00
abstract：:Developing strategies that promote the resolution of vascular inflammation and atherosclerosis remains a major therapeutic challenge. Here, we show that exosomes produced by naive bone marrow-derived macrophages (BMDM-exo) contain anti-inflammatory microRNA-99a/146b/378a that are further increased in exosomes produced...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107881
更新日期：2020-07-14 00:00:00
abstract：:Transcription-factor-induced reprogramming of somatic cells to pluripotency is a very inefficient process, probably due to the existence of important epigenetic barriers that are imposed during differentiation and that contribute to preserving cell identity. In an effort to decipher the molecular nature of these barri...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.02.029
更新日期：2013-04-25 00:00:00
abstract：:Viral vectors enable foreign proteins to be expressed in brains of non-genetic species, including non-human primates. However, viruses targeting specific neuron classes have proved elusive. Here we describe viral promoters and strategies for accessing GABAergic interneurons and their molecularly defined subsets in the...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.02.011
更新日期：2019-03-05 00:00:00
abstract：:We describe a mechanism by which the anti-apoptotic B cell lymphoma 2 (Bcl-2) protein is downregulated to induce apoptosis. ARTS (Sept4_i2) is a tumor suppressor protein that promotes cell death through specifically antagonizing XIAP (X-linked inhibitor of apoptosis). ARTS and Bcl-2 reside at the outer mitochondrial m...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.09.052
更新日期：2017-10-10 00:00:00
abstract：:Interleukin-38 (IL-38) is a cytokine of the IL-1 family with a role in chronic inflammation. However, its main cellular targets and receptors remain obscure. IL-38 is highly expressed in the skin and downregulated in psoriasis patients. We report an investigation in cellular targets of IL-38 during the progression of ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.03.082
更新日期：2019-04-16 00:00:00
