Abstract：
:High-throughput single-cell RNA sequencing (scRNA-seq) has become a frequently used tool to assess immune cell heterogeneity. Recently, the combined measurement of RNA and protein expression was developed, commonly known as cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq). Acquisition of protein expression data along with transcriptome data resolves some of the limitations inherent to only assessing transcripts but also nearly doubles the sequencing read depth required per single cell. Furthermore, there is still a paucity of analysis tools to visualize combined transcript-protein datasets. Here, we describe a targeted transcriptomics approach that combines an analysis of over 400 genes with simultaneous measurement of over 40 proteins on 2 × 104 cells in a single experiment. This targeted approach requires only about one-tenth of the read depth compared to a whole-transcriptome approach while retaining high sensitivity for low abundance transcripts. To analyze these multi-omic datasets, we adapted one-dimensional soli expression by nonlinear stochastic embedding (One-SENSE) for intuitive visualization of protein-transcript relationships on a single-cell level.
journal_name
Cell Repjournal_title
Cell reportsauthors
Mair F,Erickson JR,Voillet V,Simoni Y,Bi T,Tyznik AJ,Martin J,Gottardo R,Newell EW,Prlic Mdoi
10.1016/j.celrep.2020.03.063subject
Has Abstractpub_date
2020-04-07 00:00:00pages
107499issue
1issn
2211-1247pii
S2211-1247(20)30388-0journal_volume
31pub_type
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