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Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells.

Abstract：

:Precise rare-cell technologies require the blood to be processed immediately or be stabilized with fixatives. Such restrictions limit the translation of circulating tumor cell (CTC)-based liquid biopsy assays that provide accurate molecular data in guiding clinical decisions. Here we describe a method to preserve whole blood in its minimally altered state by combining hypothermic preservation with targeted strategies that counter cooling-induced platelet activation. Using this method, whole blood preserved for up to 72 h can be readily processed for microfluidic sorting without compromising CTC yield and viability. The tumor cells retain high-quality intact RNA suitable for single-cell RT-qPCR as well as RNA-Seq, enabling the reliable detection of cancer-specific transcripts including the androgen-receptor splice variant 7 in a cohort of prostate cancer patients with an overall concordance of 92% between fresh and preserved blood. This work will serve as a springboard for the dissemination of diverse blood-based diagnostics.

journal_name

Nat Commun

journal_title

Nature communications

authors

Wong KHK,Tessier SN,Miyamoto DT,Miller KL,Bookstaver LD,Carey TR,Stannard CJ,Thapar V,Tai EC,Vo KD,Emmons ES,Pleskow HM,Sandlin RD,Sequist LV,Ting DT,Haber DA,Maheswaran S,Stott SL,Toner M

doi

10.1038/s41467-017-01705-y

subject

Has Abstract

pub_date

2017-11-23 00:00:00

pages

1733

issue

1

issn

2041-1723

pii

10.1038/s41467-017-01705-y

journal_volume

8

pub_type

杂志文章

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