Abstract:
:The efficient selection and isolation of individual cells of interest from a mixed population is desired in many biomedical and clinical applications. Here we show the concept of using photoswitchable semiconducting polymer dots (Pdots) as an optical 'painting' tool, which enables the selection of certain adherent cells based on their fluorescence, and their spatial and morphological features, under a microscope. We first develop a Pdot that can switch between the bright (ON) and dark (OFF) states reversibly with a 150-fold contrast ratio on irradiation with ultraviolet or red light. With a focused 633-nm laser beam that acts as a 'paintbrush' and the photoswitchable Pdots as the 'paint', we select and 'paint' individual Pdot-labelled adherent cells by turning on their fluorescence, then proceed to sort and recover the optically marked cells (with 90% recovery and near 100% purity), followed by genetic analysis.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Kuo CT,Thompson AM,Gallina ME,Ye F,Johnson ES,Sun W,Zhao M,Yu J,Wu IC,Fujimoto B,DuFort CC,Carlson MA,Hingorani SR,Paguirigan AL,Radich JP,Chiu DTdoi
10.1038/ncomms11468subject
Has Abstractpub_date
2016-04-27 00:00:00pages
11468issn
2041-1723pii
ncomms11468journal_volume
7pub_type
杂志文章abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-020-15500-9
更新日期:2020-03-31 00:00:00
abstract::Repeated cell divisions and aging impair stem cell function. However, the mechanisms by which this occurs are not fully understood. Here we show that protection of telomeres 1A (Pot1a), a component of the Shelterin complex that protects telomeres, improves haematopoietic stem cell (HSC) activity during aging. Pot1a is...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00935-4
更新日期:2017-10-06 00:00:00
abstract::The direct response of the tropical mixed layer to near-inertial waves (NIWs) has only rarely been observed. Here, we present upper-ocean turbulence data that provide evidence for a strongly elevated vertical diffusive heat flux across the base of the mixed layer in the presence of a NIW, thereby cooling the mixed lay...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17601-x
更新日期:2020-07-31 00:00:00
abstract::The coupled translocation of transfer RNA and messenger RNA through the ribosome entails large-scale structural rearrangements, including step-wise movements of the tRNAs. Recent structural work has visualized intermediates of translocation induced by elongation factor G (EF-G) with tRNAs trapped in chimeric states wi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8442
更新日期:2015-06-15 00:00:00
abstract::Omniphobic membranes are attractive for membrane distillation (MD) because of their superior wetting resistance. However, a design framework for MD membrane remains incomplete, due to the complexity of omniphobic membrane fabrication and the lack of fundamental relationship between wetting resistance and water vapor p...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11209-6
更新日期:2019-07-19 00:00:00
abstract::The phosphorylation of eIF4E1 at serine 209 by MNK1 or MNK2 has been shown to initiate oncogenic mRNA translation, a process that favours cancer development and maintenance. Here, we interrogate the MNK-eIF4E axis in diffuse large B-cell lymphoma (DLBCL) and show a distinct distribution of MNK1 and MNK2 in germinal ce...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6413
更新日期:2014-11-18 00:00:00
abstract::Ultrashort optical pulses propagating in a dissipative nonlinear system can interact and bind stably, forming optical soliton molecules. Soliton molecules in ultrafast lasers are under intense research focus and present striking analogies with their matter molecules counterparts. The recent development of real-time sp...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08755-4
更新日期:2019-02-19 00:00:00
abstract::Survival following a diagnosis of multiple myeloma (MM) varies between patients and some of these differences may be a consequence of inherited genetic variation. In this study, to identify genetic markers associated with MM overall survival (MM-OS), we conduct a meta-analysis of four patient series of European ancest...
journal_title:Nature communications
pub_type: 杂志文章,meta分析
doi:10.1038/ncomms10290
更新日期:2016-01-08 00:00:00
abstract::Explosive energy release is a common phenomenon occurring in magnetized plasma systems ranging from laboratories, Earth's magnetosphere, the solar corona and astrophysical environments. Its physical explanation is usually attributed to magnetic reconnection in a thin current sheet. Here we report the important role of...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1753
更新日期:2012-03-20 00:00:00
abstract::Boron has become the principle proxy for the release of seawater-derived fluids into arc volcanics, linked to cross-arc variations in boron content and isotopic ratio. Because all ocean floor serpentinites so far analysed are strongly enriched in boron, it is generally assumed that if the uppermost slab mantle is hydr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07064-6
更新日期:2018-11-02 00:00:00
abstract::ASXL1 mutations occur frequently in myeloid neoplasms and are associated with poor prognosis. However, the mechanisms by which mutant ASXL1 induces leukaemogenesis remain unclear. In this study, we report mutually reinforcing effects between a C-terminally truncated form of mutant ASXL1 (ASXL1-MT) and BAP1 in promotin...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05085-9
更新日期:2018-07-16 00:00:00
abstract::Myoblast fusion is an indispensable step for skeletal muscle development, postnatal growth, and regeneration. Myeloid differentiation primary response gene 88 (MyD88) is an adaptor protein that mediates Toll-like receptors and interleukin-1 receptor signaling. Here we report a cell-autonomous role of MyD88 in the regu...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01866-w
更新日期:2017-11-20 00:00:00
abstract::Vortices in quantum condensates exist owing to a macroscopic phase coherence. Here we show, both experimentally and theoretically, that a quantum vortex with a well-defined core can exist in a rather thick normal metal, proximized with a superconductor. Using scanning tunneling spectroscopy we reveal a proximity vorte...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04582-1
更新日期:2018-06-11 00:00:00
abstract::Functional depletion of the alternative splicing factors Muscleblind-like (MBNL 1 and 2) is at the basis of the neuromuscular disease myotonic dystrophy type 1 (DM1). We previously showed the efficacy of miRNA downregulation in Drosophila DM1 model. Here, we screen for miRNAs that regulate MBNL1 and MBNL2 in HeLa cell...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04892-4
更新日期:2018-06-26 00:00:00
abstract::Antibody cocktails represent a promising approach to prevent SARS-CoV-2 escape. The determinants for selecting antibody combinations and the mechanism that antibody cocktails prevent viral escape remain unclear. We compared the critical residues in the receptor-binding domain (RBD) used by multiple neutralizing antibo...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20789-7
更新日期:2021-01-20 00:00:00
abstract::Through the success of deep learning in various domains, artificial neural networks are currently among the most used artificial intelligence methods. Taking inspiration from the network properties of biological neural networks (e.g. sparsity, scale-freeness), we argue that (contrary to general practice) artificial ne...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04316-3
更新日期:2018-06-19 00:00:00
abstract::Plant halogenated natural products are rare and harbor various interesting bioactivities, yet the biochemical basis for the involved halogenation chemistry is unknown. While a handful of Fe(II)- and 2-oxoglutarate-dependent halogenases (2ODHs) have been found to catalyze regioselective halogenation of unactivated C-H ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15777-w
更新日期:2020-04-20 00:00:00
abstract::The diploid nature of the human genome is neglected in many analyses done today, where a genome is perceived as a set of unphased variants with respect to a reference genome. This lack of haplotype-level analyses can be explained by a lack of methods that can produce dense and accurate chromosome-length haplotypes at ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01389-4
更新日期:2017-11-03 00:00:00
abstract::The identification of causal variants in sequencing studies remains a considerable challenge that can be partially addressed by new gene-specific knowledge. Here, we integrate measures of how essential a gene is to supporting life, as inferred from viability and phenotyping screens performed on knockout mice by the In...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14284-2
更新日期:2020-01-31 00:00:00
abstract::Liquid sensors composed of ionic liquids are rising as alternatives to solid semiconductors for flexible and self-healing electronics. However, the fluidic nature may give rise to leakage problems in cases of accidental damages. Here, we proposed a liquid sensor based on a binary ionic liquid system, in which a flowin...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08433-5
更新日期:2019-02-01 00:00:00
abstract::Ion distribution in aqueous electrolytes near the interface plays a critical role in electrochemical, biological and colloidal systems, and is expected to be particularly significant inside nanoconfined regions. Electroneutrality of the total charge inside nanoconfined regions is commonly assumed a priori in solving i...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7358
更新日期:2015-02-20 00:00:00
abstract::Radiotherapy is not currently informed by the genetic composition of an individual patient's tumour. To identify genetic features regulating survival after DNA damage, here we conduct large-scale profiling of cellular survival after exposure to radiation in a diverse collection of 533 genetically annotated human tumou...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11428
更新日期:2016-04-25 00:00:00
abstract::Central spin decoherence caused by nuclear spin baths is often a critical issue in various quantum computing schemes, and it has also been used for sensing single-nuclear spins. Recent theoretical studies suggest that central spin decoherence can act as a probe of many-body physics in spin baths; however, identificati...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5822
更新日期:2014-09-10 00:00:00
abstract::The ribosome stalls on translation of polyproline sequences due to inefficient peptide bond formation between consecutive prolines. The translation factor EF-P is able to alleviate this stalling by accelerating Pro-Pro formation. However, the mechanism by which EF-P recognizes the stalled complexes and accelerates pep...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11657
更新日期:2016-05-24 00:00:00
abstract::Genomic evidence is increasingly underpinning that hybridization between taxa is commonplace, challenging our views on the mechanisms that maintain their boundaries. Here, we focus on seven catadromous eel species (genus Anguilla) and use genome-wide sequence data from more than 450 individuals sampled across the trop...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15099-x
更新日期:2020-03-18 00:00:00
abstract::Lithium metal batteries have been considerably limited by the problems of uncontrolled dendritic lithium formation and the highly reactive nature of lithium with electrolytes. Herein, we have developed functional porous bilayer composite separators by simply blade-coating polyacrylamide-grafted graphene oxide molecula...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09211-z
更新日期:2019-03-25 00:00:00
abstract::Preterm birth places infants in an adverse environment that leads to abnormal brain development and cerebral injury through a poorly understood mechanism known to involve neuroinflammation. In this study, we integrate human and mouse molecular and neuroimaging data to investigate the role of microglia in preterm white...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00422-w
更新日期:2017-09-05 00:00:00
abstract::Bone mineral density (BMD) is a measure of osteoporosis and is useful in evaluating the risk of fracture. In a genome-wide association study of BMD among 20,100 Icelanders, with follow-up in 10,091 subjects of European and East-Asian descent, we found a new BMD locus that harbours the PTCH1 gene, represented by rs2837...
journal_title:Nature communications
pub_type: 杂志文章,meta分析
doi:10.1038/ncomms10129
更新日期:2016-01-06 00:00:00
abstract::Hepatic amebiasis, predominantly occurring in men, is a focal destruction of the liver due to the invading protozoan Entamoeba histolytica. Classical monocytes as well as testosterone are identified to have important functions for the development of hepatic amebiasis in mice, but a link between testosterone and monocy...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17260-y
更新日期:2020-07-10 00:00:00
abstract::Photocatalysis has not found widespread industrial adoption, in spite of decades of active research, because the challenges associated with catalyst illumination and turnover outweigh the touted advantages of replacing heat with light. A demonstration that light can control product selectivity in complex chemical reac...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14542
更新日期:2017-02-23 00:00:00