当前位置： SCI文献检索 > JOURNAL OF ENDODONTICS期刊下所有文献 > The Antimicrobial Peptide LL-37 as a Possible Adjunct for the Proliferation and Differentiation of Dental Pulp Stem Cells.

The Antimicrobial Peptide LL-37 as a Possible Adjunct for the Proliferation and Differentiation of Dental Pulp Stem Cells.

Abstract：

INTRODUCTION:This study evaluated the biocompatibility of 5 and 10 μg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. METHODS:Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, and DSPP gene expression. RESULTS:Concentrations of 5 and 10 μg/mL of LL-37 were not cytotoxic and generally increased cell viability, especially on the third day (P < .05). The tested concentrations did not induce genotoxicity (P < .05). LL-37 did not significantly alter nitrite production at either concentration. Cell cycle analysis revealed that 10 μg/mL of LL-37 arrested cells in G0/G1 (P < .05). The control group exhibited higher numbers of cells in other phases of the cell cycle (P < .05). The expression of the DSPP protein and gene was also higher in the 10 μg/mL of LL-37 group (P < .05). CONCLUSIONS:These results demonstrated that LL-37 was biocompatible at these concentrations and increased the number of viable cells, especially during the initial period. The 10 μg/mL concentration arrested the cell cycle and increased expression of the DSPP protein and gene, which indicates that this peptide contributes to odontoblastic differentiation.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Milhan NVM,de Barros PP,de Lima Zutin EA,de Oliveira FE,Camargo CHR,Camargo SEA

doi

10.1016/j.joen.2017.08.010

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

2048-2053

issue

12

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(17)30954-8

journal_volume

43

pub_type

杂志文章

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