Abstract：
INTRODUCTION:In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesized that citric acid (CA) might be more beneficial. METHODS:TGF-β1 release from human dentin disks conditioned with either 10% CA (pH = 2) or 17% EDTA (pH = 8) and the behavior of human stem cells toward such pretreated dentin were studied. The protein concentration in conditioning solutions after 10 minutes of dentin exposure was determined using a pH-independent slot blot technique. RESULTS:There was a 5-fold higher concentration of the target protein in CA (382 ± 30 ng/disk) compared with EDTA (66 ± 3 ng/disk, P < .005). Using confocal laser scanning microscopy on immunofluorescent-labeled disks, we identified a high density of TGF-β1 in peritubular dentin after CA treatment. A migration assay showed that CA conditioning attracted significantly more stem cells toward the dentin after 24 hours compared with EDTA (P < .05) or phosphate-buffered saline (P < .005). To investigate whether the cell response to these dentin surfaces could be affected by different pretreatments, we cultured stem cells on conditioned dentin disks and found that CA had a significantly (P < .05) better effect than EDTA on cell attachment and cell survival. CONCLUSIONS:CA conditioning could be useful and may have significant benefits over current treatments.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Ivica A,Zehnder M,Mateos JM,Ghayor C,Weber FEdoi
10.1016/j.joen.2018.09.015subject
Has Abstractpub_date
2019-01-01 00:00:00pages
45-50issue
1eissn
0099-2399issn
1878-3554pii
S0099-2399(18)30659-9journal_volume
45pub_type
杂志文章abstract：INTRODUCTION:Currently, the success of periapical microsurgery is determined by the restoration of the lamina dura and the elimination of symptoms. However, inadequate site preservation may prevent later implant placement. Although not possible before, the advent of cone-beam computed tomographic imaging and computer-a...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.11.002
更新日期：2019-04-01 00:00:00
abstract：INTRODUCTION:Experimental studies show that dental pulp cells respond unfavorably to contact with resin composite restorative material. Hypothetically, in a random population, the frequency of apical periodontitis should be higher for teeth restored with resin composite than with amalgam. Therefore, the aim was to comp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.05.002
更新日期：2014-09-01 00:00:00
abstract：:The effects of various cleaning, chemical disinfection, and sterilization procedures on the cutting efficiency of endodontic instruments Unifile (De Trey, Bois Colombes, France), (Flexofile Maillefer, Ballaigues, Switzerland), and H-File (Maillefer)) were investigated. The cross-infection control treatment procedures ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80059-8
更新日期：1996-12-01 00:00:00
abstract：:A descriptive, cross sectional survey was developed to determine the preferences of endodontists when prescribing analgesics. Eleven clinical scenarios describing common endodontic diagnoses or procedures with specified severity of pain were provided. A survey was sent to 310 AAE members and 63 responded, providing a ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.07.015
更新日期：2006-12-01 00:00:00
abstract：:This study investigated the retreatment effectiveness of .04 Taper nickel-titanium rotary ProFiles. Thirty extracted single-rooted anterior teeth were instrumented and obturated with gutta-percha/Roth's Sealer using lateral condensation. They were distributed into three groups of 10 each. Retreatment for group A was d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200002000-00010
更新日期：2000-02-01 00:00:00
abstract：INTRODUCTION:The present study aimed to evaluate the antibacterial effect of a new peptide, DJK-5, used alone or mixed together with EDTA on mono- and multispecies biofilms in dentin canals covered by a smear layer with or without preceding sodium hypochlorite (NaOCl) irrigation. METHODS:One hundred twelve dentin bloc...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.018
更新日期：2018-11-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate and compare the resistance to cyclic fatigue of the new One Curve (OC) instrument in double (S-shaped) and single curvature canals compared with other nickel-titanium rotary instruments. METHODS:Size 25/.06 of OC, 2Shape (TS), Vortex Blue (VB), ProFile Vortex (PV), an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.023
更新日期：2018-11-01 00:00:00
abstract：INTRODUCTION:An accurate differential diagnosis of apical periodontitis (AP) and nasopalatine duct cyst (NPDC) should be established to define the best treatment for endodontically treated maxillary anterior teeth with apical periodontitis. Three-dimensional cone beam computed tomography (CBCT) images help to plan trea...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.11.022
更新日期：2011-03-01 00:00:00
abstract：:K-type files, Sureflex NiTi files, and FlexoFiles were evaluated morphometrically as they come from the manufacturer, and after being used to instrument root canals 1, 3, and 5 times in maxillary premolars. Evaluation was made using a stereomicroscope at x40 magnification and photomicrographs. The abnormalities found ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(98)80046-0
更新日期：1998-07-01 00:00:00
abstract：INTRODUCTION:The goal of replantation after dental avulsion is to maintain the tooth in its socket. Presence of vital periodontal ligament cells on the root surface of the replanted tooth and the immunologic status of the patient are factors that protect against root resorption. It is known that dental constituents pla...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.09.020
更新日期：2017-01-01 00:00:00
abstract：:Pulp regeneration and its clinical translation into regenerative endodontic procedures are receiving increasing research attention, leading to significant growth of the published scientific and clinical literature within these areas. Development of research strategies, which consider patient-, clinician-, and scientis...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.06.002
更新日期：2017-09-01 00:00:00
abstract：:Root canal bacteria in teeth with apical periodontitis were enumerated after extraction and incubation. Canals in 36 teeth were sampled after: S1, incubation for 2 hours (group A), 2 days (group B), 4 days (group C), and 6 days (group D); S2, subsequent incubation for 1 week; S3, canal disinfection; and S4, final incu...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.038
更新日期：2008-12-01 00:00:00
abstract：INTRODUCTION:An evaluation was made of the connective tissue reaction in rats after subcutaneous implantation of methacrylate resin-based sealers (EndoREZ [Ultradent Products, Inc, South Jordan, UT] with a polymerization accelerator and RealSeal [Sybron Dental Specialties, Orange, CA]) and Pulp Canal Sealer (Sybron Den...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.019
更新日期：2010-09-01 00:00:00
abstract：INTRODUCTION:The dental pulp is highly vascularized and innervated tissue that is uniquely designed, being highly biologically active, while being enclosed within the calcified structure of the tooth. It is well-established that the dental pulp vasculature is a key requirement for the functional performance of the toot...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.06.033
更新日期：2020-09-01 00:00:00
abstract：:Cyanoacrylate is an extremely strong bonding agent which has many applications in medicine. It is also effective in obtaining a leakproof seal of rubber dam around a structurally compromised tooth requiring endodontic therapy. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81356-7
更新日期：1992-10-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of different irrigating solutions on smear layer removal and dentinal tubule opening on root canal surfaces after post space preparation and to study whether additional ultrasonic irrigation has any effect on smear layer removal. Forty-eight anterior teeth were trea...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.01.006
更新日期：2009-04-01 00:00:00
abstract：INTRODUCTION:Autotransplantation is a viable option for treating missing teeth when a donor tooth is available. This retrospective study reports the success rate for the autotransplantation of 19 molars with complete root formation. METHODS:The study enrolled 19 patients (11 men and 8 women) in whom 19 molars were tra...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.04.028
更新日期：2010-08-01 00:00:00
abstract：:There is an increasing interest in the utility of dental pulp stem cells (DPSCs) for dentin regeneration. The mechanisms involved in DPSC differentiation remain poorly understood. The purpose of the study was to investigate the mineralization capacity of human dental pulp cells (DPCs) and identify potential markers fo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.02.009
更新日期：2007-06-01 00:00:00
abstract：INTRODUCTION:This study aimed to investigate dental pulp responses to novel bioactive glass (BG) pulp capping materials after direct pulp capping in vivo. METHODS:Novel BG pulp capping materials are composed of powder and fluid. The powder is BG (82.36% SiO2, 15.36% CaO, and 2.28% P2O5), and the fluid is provided in 2...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.011
更新日期：2017-10-01 00:00:00
abstract：INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.11.002
更新日期：2018-03-01 00:00:00
abstract：INTRODUCTION:Growth and differentiation factor-5 (GDF-5) is a multifunctional protein that regulates the development and repair in many tissues. The purpose of this study was to investigate whether GDF-5 may influence the proliferation, differentiation, and collagen turnover of human dental pulp cells. METHODS:Human d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.06.003
更新日期：2013-10-01 00:00:00
abstract：INTRODUCTION:Nonequilibrium plasma has been reported to effectively kill Enterococcus faecalis in endodontic biofilm compared with chlorhexidine digluconate (CHX). The purpose of this study was to evaluate the antimicrobial in vitro activity of modified nonequilibrium plasma with CHX against E. faecalis and multispecie...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.06.027
更新日期：2013-11-01 00:00:00
abstract：:The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37 degrees C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and acces...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200007000-00001
更新日期：2000-07-01 00:00:00
abstract：INTRODUCTION:This study evaluated the influence of cervical preflaring on the incidence of root dentin defects after root canal preparation. METHODS:Extracted human maxillary central incisors were selected and allocated to 1 control group and 12 experimental groups (n = 15). Teeth in the control group were left unprep...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.09.021
更新日期：2018-02-01 00:00:00
abstract：:Osteoradionecrosis (ORN) of the jaw is considered the most severe long-term adverse effect that can occur in radiation therapy patients. Teeth extractions and dental diseases are the main risk factors for ORN in irradiated patients. The aim of this case series was to evaluate the outcome of primary root canal treatmen...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.12.005
更新日期：2020-04-01 00:00:00
abstract：INTRODUCTION:The study was designed as a randomized double-blind trial to evaluate the anesthetic efficacy of 4% articaine with 1:100,000 epinephrine in inferior alveolar nerve block (IANB) and infiltration anesthetic techniques to anesthetize mandibular molars with irreversible pulpitis. METHODS:The study was compose...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2011.09.009
更新日期：2011-12-01 00:00:00
abstract：INTRODUCTION:The objective of this study was to compare the outcomes of initial nonsurgical root canal therapy for different tooth types provided by both endodontists and other providers. METHODS:By using an insurance company database, 487,476 initial nonsurgical root canal therapy procedures were followed from the ti...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.02.008
更新日期：2016-05-01 00:00:00
abstract：:Removing posts from root canals prior to endodontic retreatment is often a challenge. The Gonon post removing system (Thomas Extracteur De Pivots; FFDM-Pneumat, Bourges, France) is safe and efficient and can be used for anterior, bicuspid, and even molar teeth. The Gonon system is illustrated with one clinical case. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80200-6
更新日期：1989-11-01 00:00:00
abstract：:In vitro root surface temperatures produced during post space preparation were measured. The preparations were made using a GPX bur with rotational speeds of 6,500, 8,000, 9,500, 11,000, and 15,000 rpm. The overall mean temperature increase ranged from 0.66 degree to 4.81 degrees C and was greatest at 8,000 rpm. When ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80264-0
更新日期：1996-06-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to compare the shaping ability of 4 different nickel-titanium systems, considering their variation in alloy treatment, kinematics, and design, used to prepare canals with severe curvature using micro-computed tomographic analysis. METHODS:Thirty-two mesial roots of mandibular mol...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.02.018
更新日期：2019-06-01 00:00:00
