Induction of cyclooxygenase-2 mRNA and protein expression in human pulp cells stimulated with black-pigmented bacteroides.

Abstract:

:Cyclooxygenase-2 (COX-2) is induced after the activation of cells by a variety of proinflammatory agents. Recently, evidence has shown that COX-2 may play a role in the pathogenesis of pulpal inflammation. However, little is known regarding the mechanism of pulpal inflammation at the site of bacterial infection. The purpose of this study was to determine the effects of the supernatants from black-pigmented Bacteroides (Porphyromonas endodontalis, Porphyromonas gingivalis, and Prevotella intermedia) on the COX-2 expression in primary human pulp cells in vitro. Investigations of the time dependence of COX-2 mRNA expression in black-pigmented Bacteroides-treated human pulp cells revealed a rapid accumulation of the transcript, a significant signal first detectable after 1 h of exposure. In addition, black-pigmented Bacteroides also up-regulated COX-2 protein expression in human pulp cells. Data from our in vitro experiment showed that black-pigmented Bacteroides were capable of stimulating COX-2 expression in human pulp cells. These results indicate that black-pigmented Bacteroides species may play an important role in the pathogenesis of pulpal inflammation. The activation of COX-2 may be one of the distinct host degradative pathways in the pathogenesis of microbial-induced pulpal/periapical inflammation.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Chang YC,Huang FM,Yang SF,Liu CM,Lai CC,Chan Y,Hsieh YS

doi

10.1097/00004770-200304000-00002

keywords:

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

240-3

issue

4

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)60424-4

journal_volume

29

pub_type

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