Abstract：
INTRODUCTION:Regenerative endodontic procedures are an alternative treatment for immature teeth with necrotic pulps. Typically, intracanal medicaments such as triple antibiotic paste (TAP) or double antibiotic paste (DAP) and calcium hydroxide (Ca[OH](2)) are used for disinfection. However, their effect on human stem cells of the apical papilla (SCAPs) is unknown. We hypothesized that intracanal medicaments at high concentrations are toxic to SCAPs. To test this hypothesis, a cell culture assay was used. METHODS:Briefly, SCAPs were cultured and subjected to either no drug treatment or various concentrations including TAP, DAP, modified TAP (ciprofloxacin, metronidazole and cefaclor), Augmentin (Champs Pharmacy, San Antonio, TX), or Ca(OH)(2). Viable stem cells counts were obtained using an automated method of detecting trypan blue dye at 3 days after treatment. RESULTS:All 4 antibiotics significantly reduced SCAP survival in a concentration-dependent fashion. Interestingly, Ca(OH)(2) was conducive with SCAP survival at all concentrations. CONCLUSIONS:Collectively, our data show that high concentrations of antibiotics have a detrimental effect on SCAP survival, whereas lower concentrations as well as Ca(OH)(2) at all tested concentrations are conducive with SCAP survival and proliferation. These studies highlight the clinically important point that intracanal medicaments must be used at concentrations that are bactericidal while having minimal effects on stem cell viability.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Ruparel NB,Teixeira FB,Ferraz CC,Diogenes Adoi
10.1016/j.joen.2012.06.018subject
Has Abstractpub_date
2012-10-01 00:00:00pages
1372-5issue
10eissn
0099-2399issn
1878-3554pii
S0099-2399(12)00599-7journal_volume
38pub_type
杂志文章abstract：:The purpose of this study was to evaluate the influence of Hero 642 rotary Ni-Ti instruments driven at 300, 400, or 600 rpm on root canal straightening, loss of working length, and instrument breakage. Sixty mesial root canals from extracted human mandibular molars were divided into 3 groups of 20 each and were instru...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200307000-00005
更新日期：2003-07-01 00:00:00
abstract：INTRODUCTION:Fractured endodontic files present a major problem. A novel method has been proposed to retrieve fractured nickel-titanium (NiTi) endodontic files by using electrochemical dissolution. However, the effect of file dissolution on adjacent soft tissues such as the periodontal ligament (PDL) has not been inves...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.12.013
更新日期：2013-05-01 00:00:00
abstract：:No information could be found in the dental literature on the hardness or strength of the materials in root canal instruments. Hardness testing is a convenient method for examining these products, and strength properties can be deduced from available strength-hardness data. The Vickers hardness of cross-sections of st...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80274-3
更新日期：1996-02-01 00:00:00
abstract：INTRODUCTION:Osteoporosis is a major systemic disease that can significantly deteriorate the quality of life of the affected individuals. It is more common in women, particularly after menopause. Osteoporosis may be associated with alterations in oral health. Treatment of osteoporotic patients mainly involves the admin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.10.019
更新日期：2021-02-01 00:00:00
abstract：:Fusion and gemination are developmental anomalies of the dental hard tissues that might require endodontic and surgical treatment for functional, orthodontic, or esthetic reasons. In this article, we report a rare case of successful endodontic management of unilateral fused mandibular second molar with a paramolar. Th...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.06.021
更新日期：2007-10-01 00:00:00
abstract：:The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.08.001
更新日期：2006-04-01 00:00:00
abstract：:The aim of this study was to assess the cytotoxicity of pure eugenol in an in vitro method by diluting it to various concentrations in alcohol and determining the maximum noncytotoxic concentration. We used solutions of eugenol and ethyl alcohol that are soluble in water in any given proportion. The cytotoxicity of th...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80012-4
更新日期：1996-10-01 00:00:00
abstract：:Sodium hypochlorite (NaOCl) has been recommended for irrigation during root canal preparation. This investigation used scanning electron microscopy to examine instrumented and uninstrumented surfaces in the middle third of root canals following the use of several concentrations of NaOCl (5.25%, 2.5%, 1.0%, and 0.5%). ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81331-2
更新日期：1992-12-01 00:00:00
abstract：INTRODUCTION:Endodontic claims are the most frequently filed malpractice claims in dentistry. The aim of this study was to categorize and review errors and complications related to endodontic procedures that resulted in legal actions against the treating practitioner. METHODS:All dental malpractice complaints reported...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.03.030
更新日期：2010-06-01 00:00:00
abstract：INTRODUCTION:This randomized clinical trial assessed the effect of preoperative intraoral cryotherapy application on the success rate of inferior alveolar nerve blocks (IANBs) in patients with symptomatic irreversible pulpitis (SIP). METHODS:One hundred four patients with SIP were randomly distributed into 2 groups: c...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2019.05.001
更新日期：2019-08-01 00:00:00
abstract：INTRODUCTION:A limited number of in vivo studies have discussed the prevalence of middle mesial canals in root canal systems of mandibular molars. The reported results have varied between 1% and 25%, with no detailed description of the depth and direction of troughing needed to identify such small canal orifices. The o...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.09.013
更新日期：2015-02-01 00:00:00
abstract：INTRODUCTION:Hydrogen peroxide tooth bleaching is claimed to cause alterations in dental tissue structures. This study investigated the influence of the number of bleaching sessions on pulp tissue in rats. METHODS:Male Wistar rats were studied in 5 groups (groups 1S-5S) of 10 each, which differed by the number (1-5) o...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.007
更新日期：2013-12-01 00:00:00
abstract：:The purpose of this investigation was twofold: (1) to search for clinical articles pertaining to success and failure of periapical surgery and (2) to assign levels of evidence to these studies except case reports. Electronic and manual searches were conducted to identify all the literature regarding success and failur...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/01.don.0000133158.35394.8a
更新日期：2005-01-01 00:00:00
abstract：INTRODUCTION:Apical surgery is frequently indicated in maxillary first molars. Occasionally, a vascular anastomosis in the lateral maxillary sinus wall can be observed during surgery. The aim of this study was to examine the distance between the vascular bone channel (VBC) and the root apices of maxillary first molars ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.09.004
更新日期：2019-12-01 00:00:00
abstract：:The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.11.007
更新日期：2008-05-01 00:00:00
abstract：:The role of T-cells in the development of periapical lesions was investigated immunohistochemically using 16 normal (BALB/c) mice and 16 nude (BALB/c nu/nu) mice (congenitally T-cell-deficient mice). The pulp chambers of maxillar first molars of all mice were opened, and the infiltrated immunocytes (anti-Thy1.2, -Lyt-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80565-0
更新日期：1995-04-01 00:00:00
abstract：:The aim of this study was to evaluate the presence of bacterial biofilm on the external surface of the root apex in teeth with pulp necrosis, with and without radiographically visible periapical lesions, and in teeth with a vital pulp. Twenty-one teeth were extracted, eight with pulp necrosis and periapical lesions, e...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200212000-00006
更新日期：2002-12-01 00:00:00
abstract：INTRODUCTION:The aim of this prospective, randomized, double-blind clinical trial was to evaluate the effect of a preoperative, single, oral dose of diclofenac potassium (DFK) on postoperative pain and rescue analgesic intake in patients with symptomatic irreversible pulpitis in mandibular molars treated in 1 visit. M...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2020.05.008
更新日期：2020-08-01 00:00:00
abstract：:This study investigated the possibility of saliva recontamination occurring between the root canal wall and sealer through dentinal tubules exposed after the cementum was removed at the cervical level by root planing and treatment with citric acid. Thirty-four extracted human maxillary anterior teeth were randomly pla...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80024-0
更新日期：1996-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effect of chitosan-hydroxyapatite precursor (C-HA) nanocomplex conditioning on the chemical modifications at the tricalcium silicate sealer-dentin interface using time-of-flight secondary ion mass spectrometry. METHODS:Dentin slabs from human premolar root den...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.08.011
更新日期：2019-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the push-out bond strengths of different obturating materials. METHODS:Forty single-rooted human extracted teeth were used in this study. The teeth were instrumented and irrigated by using 5.25% NaOCl, 15% ethylenediaminetetraacetic acid, and sterile water. The te...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.10.026
更新日期：2010-02-01 00:00:00
abstract：:Ten extracted human maxillary first molars were selected with a variety of root curvatures. The distofacial roots were resected and the mesiofacial canals were instrumented to a size #25 K file. Three techniques were used to introduce Ca(OH)2 paste into the instrumented canals. These techniques were evaluated for thei...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)81220-3
更新日期：1992-08-01 00:00:00
abstract：INTRODUCTION:The introduction of nickel-titanium (NiTi) files into clinical practice has improved the quality of canal shaping, but increasing the curvature of the root canal (or the diameter of the master instrument that prepares the full working length) could result in more transportation, straightening, and aberrati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.06.030
更新日期：2011-10-01 00:00:00
abstract：INTRODUCTION:The radicular groove is clinically significant due to its relevance to onset of inflammatory processes in the periodontal tissues. This study examined anatomic features of radicular grooves by using micro-computed tomography (micro-CT) scans. METHODS:Eleven extracted permanent maxillary lateral incisors w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.03.002
更新日期：2011-06-01 00:00:00
abstract：INTRODUCTION:This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts. METHODS:Biodentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.11.026
更新日期：2013-04-01 00:00:00
abstract：:Coconut water is biologically pure and sterile, with a rich presence of amino acids, proteins, vitamins, and minerals. The purpose of this study was to use a collagenase-dispase assay to investigate the potential of a new storage medium, coconut water, in comparison with propolis, Hank's balanced salt solution (HBSS),...
journal_title：Journal of endodontics
pub_type： 杂志文章,收录出版
doi：10.1016/j.joen.2008.01.018
更新日期：2008-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the release of growth factors into root canal space after the irrigation procedure of regenerative endodontic procedure. METHODS:Sixty standardized root segments were prepared from extracted single-root teeth. Nail varnish was applied to all surfaces except the roo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.04.029
更新日期：2016-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.034
更新日期：2017-03-01 00:00:00
abstract：INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.11.002
更新日期：2018-03-01 00:00:00
abstract：:A case is presented in which endodontic treatment of a maxillary lateral incisor with a talon cusp and two root canals failed following a mistaken diagnosis. What was first diagnosed as an endodontic lesion was, in all probability, a primary periodontal lesion caused by the advance of bacteria from the gingival crevic...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81947-3
更新日期：1990-07-01 00:00:00
