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Interfacial Characterization of Dentin Conditioned with Chitosan Hydroxyapatite Precursor Nanocomplexes Using Time-of-flight Secondary Ion Mass Spectrometry.

Abstract：

INTRODUCTION:The purpose of this study was to evaluate the effect of chitosan-hydroxyapatite precursor (C-HA) nanocomplex conditioning on the chemical modifications at the tricalcium silicate sealer-dentin interface using time-of-flight secondary ion mass spectrometry. METHODS:Dentin slabs from human premolar root dentin were prepared, demineralized, and randomly distributed between control and C-HA nanocomplex conditioned groups. Tricalcium silicate sealer was applied, and the slabs were allowed to set in 100% humidity for 10 days. The cross-sectional area was exposed, and the sealer-dentin interface was characterized for chemical/ultrastructural evaluation with time-of-flight secondary ion mass spectrometry and transmission electron microscopy, respectively. RESULTS:Chemical analysis revealed the presence of an ion-rich layer constituted of abundant phosphates (PO2-, PO3-, and PO4-), hydroxide (OH-), and chitosan fragments (C2H4NO-, C3H4NO2-, C2H5O2+, C2H6NO+, C4H6NO2+, C5H6NO+, and C5H5O2+) on the dentin surface at the sealer-dentin interface and subsurface dentin after conditioning with C-HA nanocomplexes. In contrast, a decreased interfacial presence of calcium (Ca+) and calcium phosphates (CaPO2+, CaPO3+, CaPO4+, and Ca2PO3+) and the absence of phosphate fragments in the control were noted. Ultrastructural evaluation showed an interfacial layer (<1 μm) with interrupted mineral aggregates in the controls as opposed to a continuous (5 μm) mineral layer formation on the conditioned dentin. CONCLUSIONS:C-HA nanocomplex conditioning of dentin before tricalcium silicate sealer application resulted in a chemically modified dentin substrate with an ion-rich layer consisting of phosphate, calcium, calcium phosphates, and chitosan that chemically modified the dentin surface/subsurface.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Hashmi A,Sodhi RNS,Kishen A

doi

10.1016/j.joen.2019.08.011

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

1513-1521

issue

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(19)30625-9

journal_volume

pub_type

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Interfacial Characterization of Dentin Conditioned with Chitosan Hydroxyapatite Precursor Nanocomplexes Using Time-of-flight Secondary Ion Mass Spectrometry.