Abstract：
:The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tetracycline (25 mg/ml), doxycycline (10 mg/ml), calcium hydroxide, 1% buffered sodium hypochlorite (NaOCl1), 3% and 6% unbuffered NaOCl (NaOCl3 and NaOCl6), 2% chlorhexidine (CHX), 5% tincture iodine (TI), 2% iodine potassium iodide (IKI), chloroform (CF), 70% ethyl alcohol, 5% sodium thiosulphate, 5% citric acid or saline were added to 10 or 10 cells/ml E. faecalis ATCC 19433 for 1 h (1 wk for Ca(OH)2). Using PCR, all specimens were positive except for NaOCl3 and NaOCl6. PCR with Ca(OH)2 was positive with 10 cells/ml but negative with 10 cells/ml. The following reagents yielded negative culturing results: all antibiotics, Ca(OH)2, CHX, IKI, TI, NaOCl3, NaOCl6, and CF. BacLight nuclear staining revealed the presence of viable cells in all PCR positive, culture negative combinations, except for those with CF. Therefore, in the presence of threshold values of bacterial concentrations, all reagents tested except for NaOCl3 and NaOCl6 do not interfere with the detection of E. faecalis using PCR.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Fouad AF,Barry Jdoi
10.1097/01.don.0000152899.54247.4ekeywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
510-3issue
7eissn
0099-2399issn
1878-3554pii
S0099-2399(05)60187-2journal_volume
31pub_type
杂志文章abstract：INTRODUCTION:Speed of injection may affect the solution spread in the pterygomandibular space. It was hypothesized that speed of injection will affect the anesthetic efficacy of inferior alveolar nerve block (IANB) in patients with symptomatic irreversible pulpitis. METHODS:Fifty-nine adult volunteers who were activel...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2012.08.006
更新日期：2012-12-01 00:00:00
abstract：:This case series reports the outcomes of 8 patients (ages 9-14 years) who presented with 9 immature permanent teeth with pulpal necrosis and apical periodontitis. During treatment, 5 of the teeth were found to have at least some residual vital tissue remaining in the root canal systems. After NaOCl irrigation and medi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.03.023
更新日期：2008-07-01 00:00:00
abstract：:The purpose of the present study was to investigate the effect of laser irradiation on calcified nodule formation in human dental pulp (HDP) cells. HDP cells were irradiated once with a Ga-Al-As laser for 5 and 10 min, and calcified nodule formation was determined by von Kossa staining. The laser irradiation increased...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80395-1
更新日期：1999-01-01 00:00:00
abstract：:One hundred randomly selected mandibular incisors were examined to assess the prevalence and location of two canals and to describe the canal anatomy that may be encountered during apical surgery. Sections of the root were cut at 1, 2, and 3 mm from the apex, simulating a 20-degree beveled surgical resection. The sect...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80120-9
更新日期：1998-09-01 00:00:00
abstract：INTRODUCTION:Root resorption is a common complication after replantation following traumatic dental avulsion. Endodontic therapy combined with local and intracanal medications aims to avoid osteoclastic activity. In such cases, the application of alendronate (ALN), a bisphosphonate widely used for the treatment of bone...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.07.010
更新日期：2015-01-01 00:00:00
abstract：INTRODUCTION:Bleeding control is an important aspect in endodontic surgery. Two hemostatic techniques were compared with regard to their efficacy to bleeding control in endodontic surgery. METHODS:A randomized, 2-arm, parallel pilot study involving 30 patients with periradicular lesions was performed including the fol...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2019.04.011
更新日期：2019-08-01 00:00:00
abstract：:Bisphosphonates are commonly used in medicine to maintain bone density in patients with certain nonneoplastic diseases or cancers. A serious adverse effect of bisphosphonates that has substantial dental significance is osteonecrosis that appears to uniquely affect the mandible and maxilla without occurring in other bo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000186481.96169.cd
更新日期：2005-11-01 00:00:00
abstract：:Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde a...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000128749.50151.24
更新日期：2004-12-01 00:00:00
abstract：INTRODUCTION:This ex vivo study compared the disinfection and shaping abilities of 2 preparation protocols in C-shaped canals. METHODS:Mandibular second molars with type I C-shaped canals were pair matched based on micro-computed tomographic (micro-CT) analysis and distributed into 2 groups. The canals were contaminat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.11.007
更新日期：2020-11-18 00:00:00
abstract：:Unlike most cases of combined orthodontic and endodontic therapy that have been reported, in this case endodontic treatment with calcium hydroxide-induced calcification was utilized prior to orthodontic treatment. A 14-yr-old female required orthodontic therapy due to anterior crowding. Preoperative periapical radiogr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80290-6
更新日期：1994-05-01 00:00:00
abstract：:The purpose of this study was to compare the in vitro sealing capacity of five materials, each used as a temporary sealing agent for the walking bleach technique. All teeth received traditional biomechanical root canal instrumentation, after which the walking bleach agent was placed in the pulp chamber space. The occl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200012000-00011
更新日期：2000-12-01 00:00:00
abstract：INTRODUCTION:The treatment of immature necrotic permanent teeth presents several clinical challenges in endodontics. Regenerative endodontic procedures (REPs) permit root development, increased canal wall thickness, and apical closure. This longitudinal cohort study aimed to evaluate the long-term clinical and radiogra...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.035
更新日期：2017-03-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the effects of different curing units and 2 luting cements on the push-out bond strength of a translucent fiber post. METHODS:Thirty maxillary incisor roots were endodontically treated. Post spaces were prepared, and the smear layers were removed. Posts (FRC Postec Pl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.04.026
更新日期：2010-09-01 00:00:00
abstract：:The purpose of this study was to evaluate the dental anxiety expressed among patients undergoing endodontic treatment with respect to some social and dental parameters. The dental records of 98 patients, 42 men and 56 women who were referred for root canal treatment to an endodontist, were examined. Patients completed...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80028-9
更新日期：1998-06-01 00:00:00
abstract：:The aim of this in vitro study was to determine whether irrigation with apical negative pressure was more effective than traditional positive-pressure irrigation in eradicating Enterococcus faecalis from preshaped root canals. Fifty-four extracted mandibular molars were instrumented to produce either a non-tapered or ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.07.022
更新日期：2008-11-01 00:00:00
abstract：INTRODUCTION:The purposes of this retrospective study were to evaluate the clinical and radiographic outcomes of mineral trioxide aggregate apexification and revascularization in nonvital immature permanent teeth and to analyze factors influencing treatment outcome. METHODS:Forty-six cases (29 cases of apexification a...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.030
更新日期：2017-02-01 00:00:00
abstract：:Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200110000-00004
更新日期：2001-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.034
更新日期：2017-03-01 00:00:00
abstract：:The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37 degrees C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and acces...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200007000-00001
更新日期：2000-07-01 00:00:00
abstract：:Extracanal invasive resorption is not a well-defined phenomenon. This article describes its clinical, radiographic, and histological characteristics. Treatment of affected teeth based on the location of the resorptive defect is also described. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80056-3
更新日期：1998-07-01 00:00:00
abstract：INTRODUCTION:The purpose of this prospective, randomized, single-blinded study was to measure the degree of anesthesia obtained with 1.8 mL and 3.6 mL of 2% lidocaine with 1:100,000 epinephrine in posterior superior alveolar (PSA) nerve blocks. METHODS:Thirty-one adult subjects randomly received PSA nerve blocks of 1....
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2010.01.009
更新日期：2010-04-01 00:00:00
abstract：INTRODUCTION:Elucidating the microbial ecology of endodontic infections (EIs) is a necessary step in developing effective intracanal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population using hig...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.03.010
更新日期：2015-08-01 00:00:00
abstract：:The objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. DNA was extracted from 45 canal samples of root-filled teet...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.01.016
更新日期：2008-05-01 00:00:00
abstract：:The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Gro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200406000-00010
更新日期：2004-06-01 00:00:00
abstract：INTRODUCTION:The study was designed as a randomized double-blind trial to evaluate the anesthetic efficacy of 4% articaine with 1:100,000 epinephrine in inferior alveolar nerve block (IANB) and infiltration anesthetic techniques to anesthetize mandibular molars with irreversible pulpitis. METHODS:The study was compose...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2011.09.009
更新日期：2011-12-01 00:00:00
abstract：:This study investigated the sealing properties of cold laterally compacted gutta percha and Thermafil applied over different apically fractured rotary nickel-titanium files. Extracted human premolars were prepared by using ProTaper (Dentsply-Maillefer, Ballaigues, Switzerland) or ProFile (Dentsply-Maillefer) systems (...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.10.023
更新日期：2008-02-01 00:00:00
abstract：:The current external inflammatory root resorption treatment protocol, which uses calcium hydroxide dressing, usually comprises multiple and long-term applications. In addition to the need for multiple appointments for calcium hydroxide replacement, the long-term maintenance of this compound in the root canal weakens d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.03.019
更新日期：2015-09-01 00:00:00
abstract：:A tight and long-lasting seal of root-end fillings is of prime clinical importance. A hundred standard bovine root sections, each 3 mm high and with a central pulp lumen of 2.6 mm in diameter, were filled with five commonly used or potential root-end filling materials. At 24 h, or at 3, 6, or 12 months after filling, ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80077-0
更新日期：1998-08-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to examine the size, volume, and other parameters of preoperative periapical lesions measured from cone-beam computed tomographic (CBCT) images as potential prognostic factors in endodontic microsurgery. METHODS:A clinical database was searched for patients who had received e...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.05.006
更新日期：2016-08-01 00:00:00
abstract：INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.001
更新日期：2017-01-01 00:00:00
