Abstract:
:Whole genome DNA-DNA hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. There are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-reactions of species within a genus, and inability to detect species present in low numbers because of loss of DNA during extraction and purification. Alternatively, inadequate extraction and purification of DNA could result in false positives. Therefore, controls are required to monitor DNA loss, DNA cross-reactions, and DNA of pure cultures mixed with bacteria-free tissue to monitor for false positives. We determined that the quality of DNA extracted from histological sections of periradicular lesions is excellent for DNA-DNA hybridization. Although lesions contain large numbers of bacteria, histological sections through lesions barely contain sufficient quantity of bacteria for such analysis. This was confirmed by histological observation of sparsely distributed bacteria within lesions. Furthermore, we found that the bacteria are not distributed evenly throughout periradicular lesions, in numbers or species.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Chan S,Mohammed N,Dobeck JM,White RR,Socransky SS,Skobe Zdoi
10.1097/00004770-200407000-00014keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
518-22issue
7eissn
0099-2399issn
1878-3554pii
00004770-200407000-00014journal_volume
30pub_type
杂志文章abstract:INTRODUCTION:Pure gutta-percha (trans-1, 4-polyisoprene [TPI]) has been used extensively as a main component of gutta-percha for root canal filling. TPI has the interesting shape memory property by cross-linking, and this polymer was commercialized under the product name of SMP-2 (Kuraray Corp, Kashima, Japan). Therefo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.05.003
更新日期:2014-10-01 00:00:00
abstract::Mineral Trioxide Aggregate (MTA) has been shown in a number of experiments to be a potential root-end filling material. The purpose of this study was to examine the periradicular tissue response of monkeys to MTA and amalgam as root-end fillings. The pulps were removed from all the maxillary incisors of three monkeys....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80051-9
更新日期:1997-04-01 00:00:00
abstract::Strippings are problems that are frequent on thin and concave roots. Treatment and prognosis differ from that of a lateral root perforation because of the size, oval shape, and thin edges of the striping. We propose a two-step technique: an endodontic phase in which the root canal system is sealed with gutta-percha ov...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80069-0
更新日期:1996-12-01 00:00:00
abstract:INTRODUCTION:Failure in endodontic treatment is often caused by the persistence of microorganisms in the root canal after therapy. When treatment fails, an immune response develops that is characterized by an extensive network of immunologic mechanisms that lead to the production of cytokines and chemokines. METHODS:T...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.08.007
更新日期:2011-12-01 00:00:00
abstract:INTRODUCTION:The aim of the present study was to investigate the impact of ambient temperature on the life span of nickel-titanium (NiTi) files. METHODS:Cyclic fatigue testing of NiTi instruments was performed in a stainless steel artificial canal. During the experiment, 4 different temperatures (0°C, 20°C, 35°C, and ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.06.006
更新日期:2018-10-01 00:00:00
abstract::The purpose of this study was to evaluate the number of canals and isthmuses obturated after Cr, Er: YSGG laser treatment. Fifty-two canals were instrumented to a size 40 Profile 0.06 file combined with RC-Prep and sodium hypochlorite, or sterile water. The teeth were divided into 3 groups: (I) canals were flooded wit...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2007.05.012
更新日期:2007-09-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to investigate the effects of pretreatment of gel chlorhexidine and ethanol on the bond strength and durability of the adhesion of the fiber post relined with resin composite to the root dentin. METHODS:Sixty bovine incisor roots were divided into four groups: irrigation with phy...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.01.007
更新日期:2011-05-01 00:00:00
abstract::The purpose of this in vitro study was to evaluate the accuracy of Root ZX apex locator to determine the working length in teeth with simulated apical root resorption. Fifty extracted, single-rooted, human teeth with mature apices were used in this study. An irregular cavity defect was drilled at the apex of each toot...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200206000-00011
更新日期:2002-06-01 00:00:00
abstract:INTRODUCTION:This study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (MTA) mixed with aqueous and propylene glycol extracts of Arctium lappa L. and Casearia sylvestris Sw. METHODS:The setting-time test was performed according to American Society for Testin...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.12.023
更新日期:2017-05-01 00:00:00
abstract::The purpose of this study was to compare ultrasonic and high-speed-bur root-end preparations. Seventy-six roots from 29 bilaterally matched pairs of human teeth in cadavers were used in this study. In group 1 ultrasonic preparations were made in 38 roots and filled with amalgam. In group 2 high-speed bur preparations ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(97)80301-9
更新日期:1997-07-01 00:00:00
abstract:INTRODUCTION:The purposes of this retrospective study were to evaluate the clinical and radiographic outcomes of mineral trioxide aggregate apexification and revascularization in nonvital immature permanent teeth and to analyze factors influencing treatment outcome. METHODS:Forty-six cases (29 cases of apexification a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.030
更新日期:2017-02-01 00:00:00
abstract::The purpose of this study was to evaluate gray mineral trioxide aggregate (MTA), white MTA, and Fuji II LC cement as coronal barriers to bacterial leakage. Seventy-eight, matched, human teeth were obturated with gutta-percha. In group I, 18 pairs received a 3-mm barrier of gray or white MTA. In group II, 18 pairs rece...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200411000-00008
更新日期:2004-11-01 00:00:00
abstract::Non-surgical endodontic treatment represents a cornerstone of contemporary endodontic care. Reflecting the central importance of this therapy, there is a high number of studies that address issues related to this treatment. Unfortunately, many of these studies are preclinical "bench top" studies with largely undefined...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2009.05.019
更新日期:2009-08-01 00:00:00
abstract::A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81411-1
更新日期:1992-04-01 00:00:00
abstract:INTRODUCTION:Apical extrusion of irrigants and debris is an inherent limitation associated with cleaning and shaping of root canals and has been studied extensively because of its clinical relevance as a cause of flare-ups. Many factors affect the amount of extruded intracanal materials. The purpose of this study was t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.09.002
更新日期:2015-12-01 00:00:00
abstract:INTRODUCTION:The aim was to assess the association of inducible costimulator (ICOS) and ICOS ligand with bone destruction in apical periodontitis (AP). METHODS:Specimens from patients presenting with AP were obtained during apicoectomy and subjected to histopathologic analysis and molecular assessment of ICOS/ICOS lig...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.018
更新日期:2019-07-01 00:00:00
abstract::This study compared the cytotoxicity of MetaSEAL (Parkell Inc, Farmington, NY), a methacrylate resin-based sealer with an epoxy resin-based (AH Plus Jet; Dentsply Caulk, Milford, DE) and a zinc oxide-eugenol-based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Five-millimeter diameter disks prepared from the resp...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.06.020
更新日期:2008-09-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the kinematics of 2 reciprocating motors and compare it with manufacturers' declared values. METHODS:VDW Silver (VDW, Munich, Germany) and ATR Tecnika (Tecnika, Pistoia, Italy) were used in the study in 5 working modes: continuous rotation at 400 rpm on VDW Silver and...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.12.008
更新日期:2014-07-01 00:00:00
abstract:INTRODUCTION:In recent years, the inflammasome has been determined to play an important role in inflammatory diseases. However, the role of the inflammasome in pulpitis remains unclear. Absent in melanoma 2 (AIM2) is a type of inflammasome that recognizes cytosolic double stranded DNA and forms a caspase-1-activating i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.07.003
更新日期:2013-11-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare canal transportation and the centering ability of Reciproc (VDW, Munich, Germany), WaveOne (Dentsply Maillefer, Ballaigues, Switzerland), and EdgeFile (EdgeEndo, Albuquerque, NM) rotary systems using cone-beam computed tomographic imaging. METHODS:Ninety mesiobucca...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.09.011
更新日期:2018-12-01 00:00:00
abstract::Twenty volunteers with first molars free of restorations and caries were recruited. One molar from each arch was selected, and rubber dam was applied. Seven sites on each crown were electric pulp tested 4 times with an Elements Diagnostic Unit, and lowest threshold responses were recorded. Data were analyzed with one-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.08.006
更新日期:2007-11-01 00:00:00
abstract:INTRODUCTION:This study aimed to evaluate the frequency of dentinal microcracks observed after root canal preparation with 2 reciprocating and a conventional full-sequence rotary system using micro-computed tomographic analysis. METHODS:Thirty mesial roots of mandibular molars presenting a type II Vertucci canal confi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.02.019
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the effect of low-surface tension EDTA solutions on the push-out bond strength of resin-based sealer to young and old root canal dentin. METHODS:Root canals from 64 (n = 32 age <30 years old and n = 32 >60 years old) extracted, decoronized, single-rooted human tee...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.09.007
更新日期:2018-03-01 00:00:00
abstract:INTRODUCTION:A great number of studies performed in the last 3 decades have shown the superior mechanical features of nickel-titanium files as compared with stainless steel files. A novel file system recently developed, Gentlefile, defies these findings and claims to have superior qualities despite the fact it is made ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.12.023
更新日期:2016-03-01 00:00:00
abstract:INTRODUCTION:The introduction of nickel-titanium (NiTi) files into clinical practice has improved the quality of canal shaping, but increasing the curvature of the root canal (or the diameter of the master instrument that prepares the full working length) could result in more transportation, straightening, and aberrati...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.06.030
更新日期:2011-10-01 00:00:00
abstract::Endodontic fillings were challenged with bacterial ingress in mandibular premolars of 4 beagle dogs. Groups 1, 2, and 3 (n = 9), had canals filled with gutta-percha and sealer, gutta-percha alone, and sealer alone, respectively. After 2 wk, pulp chambers were inoculated with plaque. Group 4 (n = 9) and group 5 (n = 5)...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81120-9
更新日期:1997-09-01 00:00:00
abstract:INTRODUCTION:Calcium silicate-based materials (CSMs) are used in various endodontic procedures. The present study examined whether prolonged contact of mineralized dentin with recently commercialized versions of these materials adversely affects dentin collagen matrix integrity. METHODS:Dentin slabs prepared from extr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.01.004
更新日期:2012-06-01 00:00:00
abstract::One hundred randomly selected mandibular incisors were examined to assess the prevalence and location of two canals and to describe the canal anatomy that may be encountered during apical surgery. Sections of the root were cut at 1, 2, and 3 mm from the apex, simulating a 20-degree beveled surgical resection. The sect...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80120-9
更新日期:1998-09-01 00:00:00
abstract::The water sorption and solubility characteristics of three contemporary methacrylate resin-based endodontic sealers, EndoREZ, Epiphany, and InnoEndo, were compared with those obtained from Kerr EWT, Ketac-Endo (positive control), GuttaFlow, and AH Plus (both negative controls). Ten disks of each material were dehydrat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.03.021
更新日期:2007-08-01 00:00:00
abstract:INTRODUCTION:A ferrule on anterior endodontic-treated teeth has been evaluated using clinical trials, in vitro tests, and finite element analysis (FEA). The patient-specific FEA with a nonuniform ferrule and nonlinear contact biting load associated with clinical validation can be used to predict failure. METHODS:A pat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.024
更新日期:2017-09-01 00:00:00