Abstract：
INTRODUCTION:Failure in endodontic treatment is often caused by the persistence of microorganisms in the root canal after therapy. When treatment fails, an immune response develops that is characterized by an extensive network of immunologic mechanisms that lead to the production of cytokines and chemokines. METHODS:The objective of this study was to determine the relative messenger RNA (mRNA) expression of IFN-γ, TNF-α, IL-1β, IL-17A, IL-10, and MCP-1 in periapical dental lesions refractory to treatment. Clinical samples were taken from teeth presenting periapical lesions refractory to endodontic treatment (the experimental group) or from healthy teeth with pulp vitality (the control group). Three paper points passing through the root apex (2 mm) were used to collect the samples. The total RNA was extracted from each sample, complementary DNA was synthesized, and quantitative polymerase chain reaction analysis was performed. The Mann-Whitney U test was used to determine the statistical significance of our findings (P < .05). RESULTS:Significant differences in the levels of IFN-γ, TNF-α, IL-17A, and MCP-1 mRNA expression were observed in cases refractory to endodontic treatment as compared with the control group. The expression of IL-1β mRNA was not significantly different between the groups. The expression of IL-10 mRNA was insignificant in both the experimental and control groups. CONCLUSIONS:A significantly increased expression of TNF-α, IFN-γ, IL-17A, and MCP-1 mRNA was observed in the periapical immune response in cases of endodontic failure. These results suggest that a proinflammatory cytokine profile predominates in these types of dental lesions.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Henriques LC,de Brito LC,Tavares WL,Vieira LQ,Ribeiro Sobrinho APdoi
10.1016/j.joen.2011.08.007subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1659-62issue
12eissn
0099-2399issn
1878-3554pii
S0099-2399(11)00929-0journal_volume
37pub_type
杂志文章abstract：:This study compared the apical seal produced by four obturation techniques. Sixty-four extracted human teeth were prepared and obturated using lateral condensation of gutta-percha that was either unmodified or was dipped in chloroform, eucalyptol, or eucapercha paste. After storage in normal saline and 0.02% azide sol...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80158-X
更新日期：1989-12-01 00:00:00
abstract：:This in vitro study evaluated the microbial leakage of Cavit, IRM, and Dyract when used as temporary filling materials after root canal treatment. The degree of coronal leakage was assessed by using a microbiological marker consisting of Streptococcus faecalis and Candida albicans. For each of the two organisms, a set...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/00004770-200211000-00004
更新日期：2002-11-01 00:00:00
abstract：INTRODUCTION:Alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. The aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (S1), after chemomechanical root cana...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.12.003
更新日期：2015-04-01 00:00:00
abstract：:The purposes of this study were to determine quantitatively the amount of debris and irrigant forced in an apical direction, the frequency of apical plug development, and the time required to prepare canals when a step-back technique using K-files was compared with the .04 Taper system. Sixty-nine extracted teeth with...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80206-9
更新日期：1998-01-01 00:00:00
abstract：INTRODUCTION:The aim of this in vitro study was to compare the effectiveness of saline, 2.5% sodium hypochlorite, and 2% chlorhexidine, with or without passive ultrasonic irrigation (PUI), in debris removal from simulated canal irregularities within prepared root canals. METHODS:Ninety bovine lateral incisors were ran...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.08.006
更新日期：2014-12-01 00:00:00
abstract：:Biofilm mode of growth is a strategy in microorganisms to survive harsh growth conditions. Although previous studies have established the ability of Enterococcus faecalis to survive postendodontic environmental conditions, the effect of such conditions on the ultrastructural and physiochemical features of E. faecalis ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000164855.98346.fc
更新日期：2005-12-01 00:00:00
abstract：INTRODUCTION:Apical periodontitis is an inflammation and destruction of periapical tissues. Matrix metalloproteinase-9 (MMP-9) is thought to be involved in periapical lesion formation and progression. The aim of this study was to evaluate the lesion progression in MMP-9 knockout (KO) mice compared with that in control ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.01.003
更新日期：2014-05-01 00:00:00
abstract：:Extracanal invasive resorption is not a well-defined phenomenon. This article describes its clinical, radiographic, and histological characteristics. Treatment of affected teeth based on the location of the resorptive defect is also described. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80056-3
更新日期：1998-07-01 00:00:00
abstract：:In this study, the effects of heparin on dentinal resorption was investigated in rats. Animals were injected with 2 x 2 IU/g Na-Heparin subcutaneously for 33 days. Histopathological examination of the anterior teeth revealed capillary proliferation, congestion, and fibrosis in the pulp in addition to resorptive lacuna...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80347-1
更新日期：1999-10-01 00:00:00
abstract：INTRODUCTION:This review aimed to find the most effective oral premedication in reducing pain in adults after nonsurgical root canal therapy (NSRCT) using network meta-analysis. METHODS:The review protocol was registered in the PROSPERO database (CRD42017071899). A literature search was performed in the MEDLINE and EB...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析
doi：10.1016/j.joen.2018.10.016
更新日期：2019-04-01 00:00:00
abstract：:Because of its close proximity to teeth and periodontium, gingival squamous cell carcinoma (SCC) can sometimes mimic tooth-related benign inflammatory conditions, resulting in misdiagnosis. In this study we report a case of gingival SCC that mimicked a dentoalveolar abscess of endodontic origin in its early presentati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.08.005
更新日期：2007-02-01 00:00:00
abstract：INTRODUCTION:Difficult handling, long setting time, and potential discoloration are important drawbacks of white mineral trioxide aggregate (WMTA). The development of Biodentine, a recently developed calcium silicate-based material (CSM), has overcome some of these shortcomings; however, there are no available data on ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.12.021
更新日期：2013-04-01 00:00:00
abstract：INTRODUCTION:Elucidating the microbial ecology of endodontic infections (EIs) is a necessary step in developing effective intracanal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population using hig...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.03.010
更新日期：2015-08-01 00:00:00
abstract：INTRODUCTION:Collagen dentin matrix may represent a suitable scaffold to be remineralized in the presence of bioactive materials. The purpose of this study was to determine if experimental resin cements containing bioactive fillers may modulate matrix metalloproteinase-mediated collagen degradation of etched dentin. M...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.05.011
更新日期：2012-09-01 00:00:00
abstract：:Increased levels of interleukin-1 (IL)-1, tumor necrosis factor-alpha (TNF-alpha), IL-6, and prostaglandin E2 (PGE2) have been detected in inflamed pulp tissue. To gain further insight into the molecular pathogenesis of pulpitis, we investigated the effects of IL-1alpha or TNF-alpha and PGE2, either alone or in combin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200203000-00013
更新日期：2002-03-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of citric acid and EDTA solutions on the microhardness and the roughness of human root canal dentin. Forty-five human teeth sectioned longitudinally were used. Specimens were randomly divided into three groups of 30 teeth each and were treated as follows: (a) one mo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000136212.53475.ad
更新日期：2005-02-01 00:00:00
abstract：:With all the potential for endodontic success, the fact remains clinicians are confronted with posttreatment endodontic disease. When the elected treatment plan is endodontic nonsurgical retreatment, then the goal is to access the pulp chamber and remove materials from the root canal space and if present, address defi...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/01.don.0000145033.15701.2d
更新日期：2004-12-01 00:00:00
abstract：INTRODUCTION:Previous studies in patients with irreversible pulpitis have reported increased success of the inferior alveolar nerve block (IANB) using premedication with ketorolac. Preemptive nitrous oxide administration has also shown an increase in the success of the IANB. Recently, ketorolac has been made available ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.09.011
更新日期：2018-01-01 00:00:00
abstract：:The aim of this in vitro study was to determine whether irrigation with apical negative pressure was more effective than traditional positive-pressure irrigation in eradicating Enterococcus faecalis from preshaped root canals. Fifty-four extracted mandibular molars were instrumented to produce either a non-tapered or ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.07.022
更新日期：2008-11-01 00:00:00
abstract：:A degree of uncertainty exists about the use of cotton pellets in the pulp chamber after canal obturation. The purpose of this study was to poll practicing endodontists and generalists regarding their preference. Forty-six endodontists and an equal number of general dentists were polled in a mail survey regarding thei...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200209000-00007
更新日期：2002-09-01 00:00:00
abstract：INTRODUCTION:This case report describes the effect of unintentionally extruded mineral trioxide aggregate (MTA) into periradicular lesions and tissues on the prognosis of periradicular healing in maxillary left central incisor with periradicular lesion. METHODS:A 17-year-old girl with a large periradicular lesion from...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.11.026
更新日期：2010-04-01 00:00:00
abstract：INTRODUCTION:Proteinases play pivotal roles in Candida albicans infections. Although the yeast can colonize the pulpal environment, there is no information about the enzymatic profile of this organism. This in vitro study aimed to determine the proteolysis levels and to investigate differences in the expression of aspa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.01.012
更新日期：2015-06-01 00:00:00
abstract：:The cyclic fatigue resistance of three types of nickel-titanium rotary files was compared in a model using reciprocating axial movement. The influence of file size and taper was also investigated and fracture patterns were examined under SEM. The 10 experimental groups consisted of ProFiles, K3s, and RaCe files, size ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.013
更新日期：2006-01-01 00:00:00
abstract：:The difference in resistance to bacterial invasion into the dentinal tubules between vital and nonvital teeth has not been determined. This study was conducted to clarify the effect of vital pulp on bacterial invasion into the dentinal tubules. The specimens were 19 intact pairs of bilateral upper third molars of 19 h...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81098-8
更新日期：1995-02-01 00:00:00
abstract：INTRODUCTION:The aim of this prospective, randomized, double-blind clinical trial was to evaluate the effect of a preoperative, single, oral dose of diclofenac potassium (DFK) on postoperative pain and rescue analgesic intake in patients with symptomatic irreversible pulpitis in mandibular molars treated in 1 visit. M...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2020.05.008
更新日期：2020-08-01 00:00:00
abstract：INTRODUCTION:Revascularization is a valuable treatment in immature necrotic teeth that allows the continuation of root development. In this article we describe successful revascularization treatment of 2 necrotic immature first mandibular molars. METHODS:The clinical and radiographic examinations showed extensive coro...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2011.01.011
更新日期：2011-04-01 00:00:00
abstract：:A descriptive, cross sectional survey was developed to determine the preferences of endodontists when prescribing analgesics. Eleven clinical scenarios describing common endodontic diagnoses or procedures with specified severity of pain were provided. A survey was sent to 310 AAE members and 63 responded, providing a ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.07.015
更新日期：2006-12-01 00:00:00
abstract：INTRODUCTION:An evaluation was made of the connective tissue reaction in rats after subcutaneous implantation of methacrylate resin-based sealers (EndoREZ [Ultradent Products, Inc, South Jordan, UT] with a polymerization accelerator and RealSeal [Sybron Dental Specialties, Orange, CA]) and Pulp Canal Sealer (Sybron Den...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.019
更新日期：2010-09-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to compare the sensitivity and specificity of cone-beam computed tomography (CBCT) scans and digital periapical radiographs (PR) in detecting strip and root perforations after root canal treatment in mandibular molars. METHODS:Forty-five curved mesial roots were endodontically pr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.12.003
更新日期：2011-04-01 00:00:00
abstract：:The objective of this study was to assess and compare canal blockages and apical extrusion of dentin debris during canal shaping with eight preparation techniques. A total of 208 canals in extracted human teeth were prepared by one operator using one type of file. The techniques included were standardized, stepback wi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80443-7
更新日期：1995-03-01 00:00:00
