Abstract:
INTRODUCTION:Proteinases play pivotal roles in Candida albicans infections. Although the yeast can colonize the pulpal environment, there is no information about the enzymatic profile of this organism. This in vitro study aimed to determine the proteolysis levels and to investigate differences in the expression of aspartyl proteinase genes (Sap 1, Sap 2, and Sap 4) among various root canal strains and clinical isolates from the lingual dorsum. METHODS:The extracellular proteinase activity of 104 C. albicans samples isolated from the lingual dorsum and from necrotic root canals was measured with respect to bovine serum albumin degradation after 5 days of incubation at 37°C. We used reverse-transcription polymerase chain reaction, a highly sensitive method, to detect messenger RNA transcripts of aspartyl proteinase genes (Sap 1, Sap 2, and Sap 4). The C. albicans strain SC 5314 was used as a positive control for both experiments because it is recognized as being highly proteolytic. All tests were performed in triplicate. RESULTS:Regardless of the isolation site, all C. albicans strains produced an opaque precipitation halo around the colonies, indicating some proteinase activity. However, the production of proteinase on the plates was significantly greater (P < .05) by the endodontic samples. Sap 2 was the most commonly expressed gene in all samples. Among the root canal samples, the detection of Sap 1 transcripts was always associated with the expression of Sap 2 and Sap 4. Sap 4 gene expression was detected in all root canal samples. The simultaneous expression of the 3 investigated Sap genes (Sap 1, Sap 2, and Sap 4) was more common in strains isolated from the lingual dorsum (50%) than in those isolated from root canals (29.4%). CONCLUSIONS:The increased proteolytic activity as well as the distinct pattern of Sap expression observed among the root canal samples may suggest a pathogenic role for C. albicans in endodontic infections.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Miranda TT,Vianna CR,Rodrigues L,Rosa CA,Corrêa A Jrdoi
10.1016/j.joen.2015.01.012subject
Has Abstractpub_date
2015-06-01 00:00:00pages
841-5issue
6eissn
0099-2399issn
1878-3554pii
S0099-2399(15)00039-4journal_volume
41pub_type
杂志文章abstract:INTRODUCTION:The aim of this study was to evaluate the radiopacity of calcium aluminate cement (EndoBinder) with 3 different radiopacifiers (bismuth oxide, zinc oxide, or zirconium oxide) in comparison with gray mineral trioxide aggregate (GMTA), white MTA, and dental structures (enamel and dentin). METHODS:Eighteen t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.10.001
更新日期:2011-01-01 00:00:00
abstract::Cells expressing interleukin-1 (IL-1) were examined in rat periapical lesions using immunohistochemical methods. During the acute phase, both IL-1 alpha- and IL-1 beta-expressing cells were observed adjacent to many osteoclasts that contained large cytoplasms, and consequently the periapical lesions tended to expand. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80008-3
更新日期:1998-12-01 00:00:00
abstract::The cytotoxicity of four different calcium hydroxide-based root canal sealers (Sealapex, Apexit, CRCS, and Endoflas FS) was tested using human gingiva fibroblasts. The fibroblasts were acquired from the attached gingiva of apparently healthy patients. The sealers were allowed to set for 24 and 48 h before being covere...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81309-9
更新日期:1992-03-01 00:00:00
abstract::The purpose of this study was to compare clinical, radiographic, and histopathologic effects of Nd:YAG laser pulpotomy to formocresol pulpotomy on human primary teeth. Patients with at least two vital primary molar teeth that required pulpotomy, because of pulpal exposure to caries, were selected for this study. After...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.12.013
更新日期:2007-04-01 00:00:00
abstract:INTRODUCTION:The health of human teeth depends on the integrity of the hard tissue and the activity of the pulp and periodontal tissues, which are responsible for nutritional supply. Without the nourishing of the pulp tissue, the possibility of tooth fracture can increase. In immature permanent teeth, root development ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.10.002
更新日期:2013-02-01 00:00:00
abstract:INTRODUCTION:This study was conducted to evaluate and compare the fracture resistance of roots obturated with various contemporary canal-filling systems. METHODS:Fifty single-rooted extracted mandibular premolars were decoronated to obtain 13-mm root segments. Ten roots were left unprepared and unfilled (negative cont...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.08.008
更新日期:2011-11-01 00:00:00
abstract:INTRODUCTION:Anesthetic efficacy of inferior alveolar nerve block decreases in patients with irreversible pulpitis. It was hypothesized that premedication with nonsteroidal anti-inflammatory drugs might improve the success rates in patients with inflamed pulps. METHODS:Sixty-nine adult volunteers who were actively exp...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2009.11.010
更新日期:2010-03-01 00:00:00
abstract::Understanding the chemical and physical characteristics of set mineral trioxide aggregate (MTA) surface can provide insight into its bioactivity. The purpose of this study was to describe the surface chemistry and morphology of gray and white MTA set in the presence of fetal bovine serum (FBS). Eight MTA blocks were p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.09.013
更新日期:2008-01-01 00:00:00
abstract::An in vitro study was conducted to determine the susceptibility of the yeast Candida albicans to various intracanal irrigants and medications. The minimum inhibitory concentration (MIC) of sodium hypochlorite, hydrogen peroxide, chlorhexidine digluconate, and aqueous calcium hydroxide that is required to kill a standa...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200202000-00004
更新日期:2002-02-01 00:00:00
abstract::A palatogingival groove is an anatomic malformation that predisposes the involved tooth to a severe periodontal defect. When the condition is complicated by pulpal necrosis, affected teeth often present a dilemma in terms of diagnosis and treatment planning. In this report, we describe the case of a patient with a max...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.10.009
更新日期:2016-02-01 00:00:00
abstract::Orthodontic tooth movement has been implicated in secondary changes to the dental pulp. The purpose of this study was to correlate the effects of orthodontic tooth movement on the dental pulp by histomorphometric parameters. Four groups, each consisting of 36 male adult Sprague-Dawley strain rats, were studied with di...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81034-4
更新日期:1993-01-01 00:00:00
abstract::The purpose of this in vitro study was to evaluate quantitatively the ability of four different filling materials to seal the orifices of root canals as a secondary seal after root canal therapy. Forty extracted human molar teeth were used. The top of pulp chambers and distal halves of the roots were removed using an ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200108000-00006
更新日期:2001-08-01 00:00:00
abstract:INTRODUCTION:The aim was to assess the association of inducible costimulator (ICOS) and ICOS ligand with bone destruction in apical periodontitis (AP). METHODS:Specimens from patients presenting with AP were obtained during apicoectomy and subjected to histopathologic analysis and molecular assessment of ICOS/ICOS lig...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.018
更新日期:2019-07-01 00:00:00
abstract::Studies of endodontic outcomes suggest that fracture of the tooth after endodontic therapy may be a greater problem than endodontic reinfection. Immediate full coverage with or without a post and core is the best way to prevent fracture. Unfortunately, in many population areas, due predominantly to cost, this restorat...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2019.12.012
更新日期:2020-04-01 00:00:00
abstract:INTRODUCTION:Mixtures of clodronate with sodium hypochlorite (NaOCl) better maintain free available chlorine (FAC) than etidronate-hypochlorite mixtures. This research aimed to compare organic tissue dissolution and residual FAC between clodronate and etidronate mixtures. Additionally, clodronate-hypochlorite mixtures ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.020
更新日期:2020-02-01 00:00:00
abstract::Irreversibly inflamed pulp (IIP) constitutes both a pathophysiologic and a diagnostic challenge. Gingival crevicular fluid (GCF) samples were obtained with Periopaper strips from IIP and adjacent and contralateral teeth for interleukin-8 (CXCL8) and tumor necrosis factor-alpha (TNF-alpha) measurements. Pain intensity ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.10.022
更新日期:2008-02-01 00:00:00
abstract:INTRODUCTION:More information regarding the ability of single files to maintain the original canal position is required before adopting a single-file approach to cleaning and shaping. This study compares apical transportation in curved root canals when instrumenting with a single WaveOne (WO) file (Dentsply Tulsa Denta...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.09.021
更新日期:2014-05-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to investigate the number of roots and the morphology of the root canals of maxillary and mandibular premolars in a selected German population. METHODS:Randomly selected full-size cone-beam computed tomographic images were collected from 700 patients (45% men and 55% women, avera...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.03.044
更新日期:2017-09-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the efficacy and safety of postoperative medications in decreasing pain after nonsurgical endodontic treatment using a network meta-analytic approach. METHODS:MEDLINE, Embase, CENTRAL, CINAHL, and Scopus were searched (until July 31, 2019). Two reviewers selected ...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2020.07.002
更新日期:2020-10-01 00:00:00
abstract:INTRODUCTION:Revascularization treatment is rapidly becoming an accepted treatment alternative for the management of endodontic pathology in immature permanent teeth with necrotic dental pulps. However, the success and timing of clinical resolution of symptoms, and radiographic outcomes of interest, such as continued h...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.08.023
更新日期:2014-12-01 00:00:00
abstract:INTRODUCTION:The purpose of this in vitro study was to compare the cytotoxicity of white mineral trioxide aggregate cement (AMTA, MTA-Angelus), Brasseler Endosequence Root Repair Putty (ERRM), Dycal, and Ultra-blend Plus (UBP) by using human dermal fibroblasts and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.11.012
更新日期:2012-03-01 00:00:00
abstract::The purpose of this study was to evaluate the efficacy of digital subtraction radiography using a direct digital imaging system in the follow-up study of endodontically treated teeth. The RVG-S was used as a direct digital imaging system. The intraimage variation of the original RVG-S image caused by dark current and ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200208000-00006
更新日期:2002-08-01 00:00:00
abstract:INTRODUCTION:Candida in endodontic infections has been investigated in a large number of studies, but its role as an endodontic pathogen is still debatable. The aim of this study was to systematically review the literature on the prevalence of Candida species in root canal infections. METHODS:Extensive literature rese...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2018.07.016
更新日期:2018-11-01 00:00:00
abstract::The effects of parathyroid hormone (PTH), 1,25-dihydroxyvitamin D3, and prostaglandin E2 (PGE2) on alkaline phosphatase activity on cultured dental pulp and gingiva cells of bovine calf were compared. In pulp cells, PTH, 1,25-dihydroxyvitamin D3, and PGE2 significantly increased alkaline phosphatase activity, but no i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)82009-1
更新日期:1991-04-01 00:00:00
abstract:INTRODUCTION:Enterococcus faecalis is frequently recovered from root-filled teeth with refractory apical periodontitis. The ability of E. faecalis to form a matrix-encased biofilm contributes to its pathogenicity; however, the role of extracellular dextran and DNA in biofilm formation and its effect on the susceptibili...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.04.007
更新日期:2012-07-01 00:00:00
abstract:INTRODUCTION:The effect of rheumatoid arthritis (RA) on the healing and pathophyisiology of apical periodontitis is unknown because there is no study in the literature evaluating the association of RA with the prevalence of periapical rarefying osteitis (PAR) and endodontic treatment. Therefore, the goal of this cross-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.02.015
更新日期:2017-07-01 00:00:00
abstract:INTRODUCTION:Difficult handling, long setting time, and potential discoloration are important drawbacks of white mineral trioxide aggregate (WMTA). The development of Biodentine, a recently developed calcium silicate-based material (CSM), has overcome some of these shortcomings; however, there are no available data on ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.12.021
更新日期:2013-04-01 00:00:00
abstract::Dentinal chips and different biocompatible materials have been used in the past as apical plugs to enhance healing and simplify obturation. The purpose of this study was to evaluate bone reactions to implants of hydroxyapatite, collagen, and calcium hydroxide, alone or in different combinations. The materials were imp...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(07)80175-0
更新日期:1990-10-01 00:00:00
abstract::Endodontic microsurgery has reduced the degree of treatment trauma compared with traditional apical surgery and further increased the success rate of natural teeth retention. However, when root apices of the mandibular posterior teeth are far from the buccal cortical bone surface or near to the inferior alveolar nerve...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.07.033
更新日期:2020-08-11 00:00:00
abstract::The cytotoxic effects of a new epoxy resin-based root canal sealer (AH-plus), together with those of two other commonly used endodontic sealers (AH26 and zinc oxide-eugenol), have been studied in vitro on a culture of human gingival fibroblasts. Cytotoxicity was assessed by direct incubation of sealers' extracts with ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200008000-00008
更新日期:2000-08-01 00:00:00