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Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing.

Abstract：

INTRODUCTION:Elucidating the microbial ecology of endodontic infections (EIs) is a necessary step in developing effective intracanal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population using high-throughput sequencing methods. METHODS:16S amplicon pyrosequencing of 48 root canal bacterial samples was conducted, and sequencing data were analyzed using an oral microbiome-specific and a generic (Greengenes) database. Bacterial abundance and diversity were examined by EI type (primary or persistent), and statistical analysis was performed by using non-parametric and parametric tests accounting for clustered data. RESULTS:Bacteroidetes was the most abundant phylum in both infection groups. Significant, albeit weak associations of bacterial diversity were found, as measured by UniFrac distances with infection type (analyses of similarity, R = 0.087, P = .005) and symptoms (analyses of similarity, R = 0.055, P = .047). Persistent infections were significantly enriched for Proteobacteria and Tenericutes compared with primary ones; at the genus level, significant differences were noted for 14 taxa, including increased enrichment of persistent infections for Lactobacillus, Streptococcus, and Sphingomonas. More but less abundant phyla were identified using the Greengenes database; among those, Cyanobacteria (0.018%) and Acidobacteria (0.007%) were significantly enriched among persistent infections. Persistent infections showed higher phylogenetic diversity (PD) (asymptomatic: PD = 9.2, standard error [SE] = 1.3; symptomatic: PD = 8.2, SE = 0.7) compared with primary infections (asymptomatic: PD = 5.9, SE = 0.8; symptomatic: PD = 7.4, SE = 1.0). CONCLUSIONS:The present study revealed a high bacterial diversity of EI and suggests that persistent infections may have more diverse bacterial communities than primary infections.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Tzanetakis GN,Azcarate-Peril MA,Zachaki S,Panopoulos P,Kontakiotis EG,Madianos PN,Divaris K

doi

10.1016/j.joen.2015.03.010

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

1226-33

issue

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(15)00242-3

journal_volume

pub_type

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Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing.