Polymerase chain reaction of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in primary endodontic infections.

Abstract:

:The aim of this study was to investigate the correlation between endodontic clinical signs and symptoms and the presence of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia or their association by nested polymerase chain reaction assay. Microbial samples were taken from 50 cases with necrotic pulp tissues in primary infections. DNA was extracted from the samples, which were analyzed for the presence of three endodontic pathogens by using species-specific primers. P gingivalis, T denticola, and T forsythia were detected in 46%, 38%, and 22% of the symptomatic cases, respectively. The bacterial complex composed by T forsythia, P gingivalis, and T denticola was found in 14% of the cases with spontaneous pain, tenderness to percussion, swelling, and pain on palpation. The high prevalence of P gingivalis, T denticola, and T forsythia in the samples examined suggests that these bacteria are related to the etiology of symptomatic periradicular diseases.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Gomes BP,Montagner F,Jacinto RC,Zaia AA,Ferraz CC,Souza-Filho FJ

doi

10.1016/j.joen.2007.05.017

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

1049-52

issue

9

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(07)00512-2

journal_volume

33

pub_type

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