Abstract：
INTRODUCTION:Interferon regulatory factor 8 (IRF8) is a critical transcription factor in innate immune responses that regulates the development and function of myeloid cells. Human periapical lesions are caused by endodontic microbial infections. However, the presence of IRF8 in human periapical lesions remains elusive. This study aims to explore the expression of IRF8 in human periapical lesions and the possible association of IRF8 with macrophages, nuclear factor kappa B (NF-κB) signaling, and the autophagy process. METHODS:Thirty-nine human periapical tissues, including healthy control tissues (n = 15), radicular cysts (RCs, n = 11), and periapical granulomas (PG, n = 13), were examined. Tissues were fixed in paraformaldehyde and analyzed. The inflammatory infiltrates of lesions were evaluated by hematoxylin-eosin, and the expression of IRF8 was analyzed by immunohistochemistry. Double immunofluorescence assessment was performed to colocalize IRF8 with CD68, NF-κB p65, and LC3B. RESULTS:The expression of IRF8 was significantly higher in RCs and PGs than in the healthy control group, but no significant difference was found between RCs and PGs. There were significantly more IRF8-CD68 double-positive cells in RCs and PGs than in the healthy control group, but no significant difference was observed between RCs and PGs. Double-labeling analysis of IRF8 with NF-κB and LC3B indicated that IRF8 expression is associated with NF-κB signaling and the autophagy process during periapical lesions. CONCLUSIONS:IRF8 could be observed and might possibly be involved in macrophages in the development of periapical lesions.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Yu J,Liu M,Zhu L,Zhu S,Lv F,Wang Y,Wang L,Peng Bdoi
10.1016/j.joen.2018.04.015subject
Has Abstractpub_date
2018-08-01 00:00:00pages
1276-1282issue
8eissn
0099-2399issn
1878-3554pii
S0099-2399(18)30289-9journal_volume
44pub_type
杂志文章abstract：INTRODUCTION:The aim of this study was to evaluate and compare the resistance to cyclic fatigue of the new One Curve (OC) instrument in double (S-shaped) and single curvature canals compared with other nickel-titanium rotary instruments. METHODS:Size 25/.06 of OC, 2Shape (TS), Vortex Blue (VB), ProFile Vortex (PV), an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.023
更新日期：2018-11-01 00:00:00
abstract：:A case is presented in which a previous partial root canal treatment on a mandibular molar developed internal resorption of the distal canal. The case was followed at the University of Oklahoma student clinic and endodontic treatment completed in the mesial canals, with calcium hydroxide placed in the distal canal for...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200107000-00010
更新日期：2001-07-01 00:00:00
abstract：:The inferior alveolar nerve is sometimes affected by periapical pathoses and mandibular cysts. However, mandibular intraosseous lesions have not been reported to disturb the lingual nerve. A case of simultaneous paresthesia of the right lingual nerve and the right inferior alveolar nerve is presented. The possible mec...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000158011.64829.7b
更新日期：2005-10-01 00:00:00
abstract：INTRODUCTION:A limited number of in vivo studies have discussed the prevalence of middle mesial canals in root canal systems of mandibular molars. The reported results have varied between 1% and 25%, with no detailed description of the depth and direction of troughing needed to identify such small canal orifices. The o...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.09.013
更新日期：2015-02-01 00:00:00
abstract：:The purpose of this investigation was twofold: (1) to search for clinical articles pertaining to success and failure of periapical surgery and (2) to assign levels of evidence to these studies except case reports. Electronic and manual searches were conducted to identify all the literature regarding success and failur...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/01.don.0000133158.35394.8a
更新日期：2005-01-01 00:00:00
abstract：INTRODUCTION:Revascularization is a valuable treatment in immature necrotic teeth that allows the continuation of root development. In this article we describe successful revascularization treatment of 2 necrotic immature first mandibular molars. METHODS:The clinical and radiographic examinations showed extensive coro...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2011.01.011
更新日期：2011-04-01 00:00:00
abstract：:The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.02.009
更新日期：2018-05-01 00:00:00
abstract：:The purpose of this study was to quantify the amount of remaining gutta-percha/sealer after retreatment of post space prepared teeth obturated with a lateral condensation technique or with Thermafil with plastic and metallic carrier. The time required for retreatment was also recorded. Forty-five extracted mandibular ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80019-1
更新日期：1994-01-01 00:00:00
abstract：INTRODUCTION:The improvement of regenerative endodontic procedures requires an understanding of the key clinical questions combined with a fundamental biological knowledge of how the dental tissues behave during health, disease, and repair. Therefore, partnerships between clinicians and basic scientists are essential t...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2020.06.019
更新日期：2020-09-01 00:00:00
abstract：:The aim of this study was to investigate the extent of calcium ion (Ca(2+)) removal on root dentin after treatment with 1.25% NaOCl, 2.5% NaOCl, 5.25% NaOCl, 2% chlorhexidine (CHX), 2% iodine potassium iodide (IKI), and MTAD in 3 immersion time periods. Extracted human mandibular premolars were bisected longitudinally...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.11.016
更新日期：2009-02-01 00:00:00
abstract：:The mechanisms of odontogenic pain are complex and incompletely understood. Cases of irreversible pulpitis are thought to represent a localized inflammatory response to bacterial challenge in dental pulp tissue. The presenting symptoms are classically defined by exaggerated painful episodes to thermal stimuli that may...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.01.023
更新日期：2007-05-01 00:00:00
abstract：:Teeth are vital sensory organs that contribute to our daily activities of living. Unfortunately, teeth can be lost for several reasons including trauma, caries, and periodontal disease. Although dental trauma injuries and caries are more frequently encountered in a younger population, tooth loss because of periodontal...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.05.014
更新日期：2019-12-01 00:00:00
abstract：INTRODUCTION:The purpose of the present study was to evaluate the thickness and the anatomic characteristics of the Schneiderian membrane and cortical bone using limited cone beam computed tomography (CBCT) scannning in patients referred for planning of apical surgery of maxillary molars. METHODS:This controlled study...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.09.023
更新日期：2012-01-01 00:00:00
abstract：INTRODUCTION:The present study quantitatively assessed tissue regeneration after regenerative endodontic procedures (REPs) with 2 different apical preparation sizes in mature teeth using magnetic resonance imaging (MRI). METHODS:Eighteen maxillary anterior mature necrotic teeth with periapical lesions were selected fo...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2020.01.026
更新日期：2020-05-01 00:00:00
abstract：INTRODUCTION:Difficult handling, long setting time, and potential discoloration are important drawbacks of white mineral trioxide aggregate (WMTA). The development of Biodentine, a recently developed calcium silicate-based material (CSM), has overcome some of these shortcomings; however, there are no available data on ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.12.021
更新日期：2013-04-01 00:00:00
abstract：INTRODUCTION:Growth and differentiation factor-5 (GDF-5) is a multifunctional protein that regulates the development and repair in many tissues. The purpose of this study was to investigate whether GDF-5 may influence the proliferation, differentiation, and collagen turnover of human dental pulp cells. METHODS:Human d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.06.003
更新日期：2013-10-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the number of roots and the morphology of the root canals of maxillary and mandibular premolars in a selected German population. METHODS:Randomly selected full-size cone-beam computed tomographic images were collected from 700 patients (45% men and 55% women, avera...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.044
更新日期：2017-09-01 00:00:00
abstract：:Dental pulp is a soft mesenchymal tissue densely innervated by afferent (sensory) fibers, sympathetic fibers, and parasympathetic fibers. This complexity in pulp innervation has motivated numerous investigations regarding how these 3 major neuronal systems regulate pulp physiology and pathology. Most of this research ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2008.03.010
更新日期：2008-07-01 00:00:00
abstract：:The influence of different obturation techniques (lateral condensation, Obtura, and Thermafil) on root strains was assessed in 27 upper central incisors using strain gauges mounted on the coronal and apical one-thirds of the root surface. The vertical load applied with each obturation technique was also measured. Comp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81008-3
更新日期：1995-06-01 00:00:00
abstract：INTRODUCTION:It has been widely accepted that dental pulp stem cells (DPSCs), which are a class of self-renewal and differentiation potential of adult stem cells, play an important role in the repair procession of pulp's inflammation. We investigated whether tumor necrosis factor alpha (TNF-α) could induce the prolifer...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.02.020
更新日期：2015-07-01 00:00:00
abstract：:A retrograde root-end cover with a special resin composite (Retroplast) combined with the dentin bonding agent Gluma (Bayer AG) has been used since 1984 by the authors. Its content of silver, added to promote radiopacity, has been found to lower the working time and storage stability of the composite and might cause d...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章
doi：10.1016/S0099-2399(96)80082-3
更新日期：1996-09-01 00:00:00
abstract：:Various cells and inflammatory mediators are involved in the initial pulpal responses to caries. This review focuses on the cellular, neuronal, and vascular components of pulpal innate responses to caries. Discussion will include dentinal fluid, odontoblasts, neuropeptides, and neurogenic inflammation, which are not c...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.01.001
更新日期：2007-06-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to assess 2 cone-beam computed tomographic systems on the detection of artificially induced vertical root fractures (VRFs) and artifact intensity using birooted teeth restored with different intracanal materials. METHODS:The sample consisted of 20 extracted birooted premolars. Ro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.10.028
更新日期：2020-02-01 00:00:00
abstract：BACKGROUND:The aim of this in vitro study was to evaluate the depth of penetration of 4 different endodontic resin sealers into the radicular dentinal tubules with the aid of confocal microscopy. METHODS:Eighty single-rooted teeth were instrumented and divided into 4 groups composed of 20 teeth each. The samples were ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.05.017
更新日期：2012-10-01 00:00:00
abstract：INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.001
更新日期：2017-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the push-out bond strengths of different obturating materials. METHODS:Forty single-rooted human extracted teeth were used in this study. The teeth were instrumented and irrigated by using 5.25% NaOCl, 15% ethylenediaminetetraacetic acid, and sterile water. The te...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.10.026
更新日期：2010-02-01 00:00:00
abstract：:The bond strength of ActiV GP root canal filling system and gutta-percha/AH plus sealer when used after final rinse with different irrigation protocols was evaluated in this study. Forty roots were randomly divided into four groups (n = 10) according to the final irrigation regimen: group 1, 5 mL 17% EDTA; group 2, 5 ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.01.003
更新日期：2009-04-01 00:00:00
abstract：INTRODUCTION:An evaluation was made of the connective tissue reaction in rats after subcutaneous implantation of methacrylate resin-based sealers (EndoREZ [Ultradent Products, Inc, South Jordan, UT] with a polymerization accelerator and RealSeal [Sybron Dental Specialties, Orange, CA]) and Pulp Canal Sealer (Sybron Den...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.019
更新日期：2010-09-01 00:00:00
abstract：INTRODUCTION:Apical extrusion of irrigants and debris is an inherent limitation associated with cleaning and shaping of root canals and has been studied extensively because of its clinical relevance as a cause of flare-ups. Many factors affect the amount of extruded intracanal materials. The purpose of this study was t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.09.002
更新日期：2015-12-01 00:00:00
abstract：INTRODUCTION:This study aimed to examine the dynamic recovery of established multispecies biofilms of oral bacteria after an initial treatment by D-enantiomeric peptide DJK-5, L-enantiomeric peptide 1018, or chlorhexidine digluconate (CHX). METHODS:Oral biofilms from 2 donors were grown on collagen-coated hydroxyapati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.11.020
更新日期：2020-11-25 00:00:00
