Abstract：
INTRODUCTION:This study aimed to examine the dynamic recovery of established multispecies biofilms of oral bacteria after an initial treatment by D-enantiomeric peptide DJK-5, L-enantiomeric peptide 1018, or chlorhexidine digluconate (CHX). METHODS:Oral biofilms from 2 donors were grown on collagen-coated hydroxyapatite disks for 3 weeks and exposed to DJK-5, 1018, and 2% CHX for 3 minutes. Immediately after treatment and 1, 2, 3, 5, 7, 8, and 12 weeks after exposure, the biofilm volume and the volume ratio of dead and live bacteria in biofilms were assessed by confocal laser scanning microscopy using a live/dead viability stain. Results were examined by 1-way analysis of variance and post hoc multiple comparisons to determine significance at a P < .05 significance level. RESULTS:DJK-5 killed almost 80% of biofilms in 3 minutes and maintained this high level of dead bacteria for 1 week. The proportion of viable bacteria in DJK-5-treated biofilms returned to the pretreatment level after 12 weeks. The biovolume of DJK-5-treated biofilm remained significantly lower than that of biofilms after CHX and no treatment throughout the 12-week follow-up period (P < .001). The proportion of dead bacteria was higher in biofilms exposed to DJK-5 than with 1018 or CHX for 8 weeks after the exposure (P < .001). The proportion of dead bacteria almost doubled to 46%-52% during the first 7 days after the 3-minute exposure to CHX and peptide 1018. The timeline of biofilm recovery was slow but similar after exposure to CHX and the 2 peptides. CONCLUSIONS:ecovery time after exposure to DJK-5 was longer than that after exposure to 1018 and CHX. Peptide 1018 showed a delayed, continued antibacterial effect similar to that of 2% CHX against the biofilm microbes.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Zhang T,Xia L,Wang Z,Hancock REW,Haapasalo Mdoi
10.1016/j.joen.2020.11.020subject
Has Abstractpub_date
2020-11-25 00:00:00eissn
0099-2399issn
1878-3554pii
S0099-2399(20)30926-2pub_type
杂志文章abstract：INTRODUCTION:Autotransplantation is a viable option for treating missing teeth when a donor tooth is available. This retrospective study reports the success rate for the autotransplantation of 19 molars with complete root formation. METHODS:The study enrolled 19 patients (11 men and 8 women) in whom 19 molars were tra...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.04.028
更新日期：2010-08-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to compare the transportation and centering abilities of the ProGlider (PG; Dentsply-Sirona, Ballaigues, Switzerland), WaveOne Gold Glider (WOGG; Dentsply-Sirona), and R-Pilot (RP; VDW, Munich, Germany) file systems and the changes in the structure model index (SMI), surface area,...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.02.012
更新日期：2019-06-01 00:00:00
abstract：INTRODUCTION:The aim of this retrospective study was to evaluate the outcome of endodontic microsurgery and to examine prognostic factors related to healing. METHODS:The clinical records of all patients who had undergone endodontic microsurgery from 1997-2003 at the National Dental Centre of Singapore were examined. T...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.04.005
更新日期：2014-08-01 00:00:00
abstract：INTRODUCTION:Cell migration is an important step in pulpal wound healing. Although components in the resin-based dental materials are known to have adverse effects on pulp wound healing including proliferation and mineralization, their effects on cell migration have been scarcely examined. Here, we investigated the eff...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.06.004
更新日期：2013-09-01 00:00:00
abstract：:This study was conducted to evaluate the influence of immediate versus delayed immersion time, and passive dye immersion versus centrifuged dye on apical leakage measurements. Eighty-four extracted human teeth with single straight canals were instrumented and divided into four experimental groups of 20 teeth each plus...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81364-6
更新日期：1993-07-01 00:00:00
abstract：INTRODUCTION:The effect of rheumatoid arthritis (RA) on the healing and pathophyisiology of apical periodontitis is unknown because there is no study in the literature evaluating the association of RA with the prevalence of periapical rarefying osteitis (PAR) and endodontic treatment. Therefore, the goal of this cross-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.015
更新日期：2017-07-01 00:00:00
abstract：INTRODUCTION:The goal of replantation after dental avulsion is to maintain the tooth in its socket. Presence of vital periodontal ligament cells on the root surface of the replanted tooth and the immunologic status of the patient are factors that protect against root resorption. It is known that dental constituents pla...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.09.020
更新日期：2017-01-01 00:00:00
abstract：:Simulated furcation perforations were repaired with and without blood contamination utilizing tooth-colored mineral trioxide aggregrate (MTA) mixed with either MTA liquid (sterile water), lidocaine, or saline. Samples underwent Instron testing at either 24 or 72 hours and at 7 days. Data were analyzed using a 3-way AN...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.09.007
更新日期：2006-05-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the antibacterial efficacy of silver nanoparticles (AgNPs) as an irrigant or medicament against Enterococcus faecalis biofilms formed on root dentin. METHODS:Dentin sections were inoculated with E. faecalis for 4 weeks to establish a standard monospecies biofilm m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.022
更新日期：2014-02-01 00:00:00
abstract：INTRODUCTION:The present study quantitatively assessed tissue regeneration after regenerative endodontic procedures (REPs) with 2 different apical preparation sizes in mature teeth using magnetic resonance imaging (MRI). METHODS:Eighteen maxillary anterior mature necrotic teeth with periapical lesions were selected fo...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2020.01.026
更新日期：2020-05-01 00:00:00
abstract：INTRODUCTION:Apical root resection is a biologically essential component in endodontic microsurgery. However, because it reduces the total root length and supported root surface, it changes the biomechanical response of the tooth. The purpose of this study was to analyze the biomechanical effect of apical root resectio...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.03.006
更新日期：2014-09-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) is a potent mitogenic and angiogenic factor involved in the development of many tissues including the teeth. We previously reported that dental pulp cells express the HGF receptor c-met and that HGF can enhance cell proliferation and differentiation. The purpose of this study was to dete...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.11.031
更新日期：2009-03-01 00:00:00
abstract：:The extraoral sinus tract may occur as a result of an inflammatory process associated with a necrotic pulp. However, several non-odontogenic disorders may also produce an extraoral sinus tract. Thus, the differential diagnosis of this clinical finding is of paramount importance in providing appropriate clinical care b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200312000-00015
更新日期：2003-12-01 00:00:00
abstract：:Intracoronal bleaching of pulpless teeth with 30% hydrogen peroxide may result in external cervical root resorption. One of several suggested etiological factors associated with this complication is the pH of the bleaching materials. Because the available data on the pH values of bleaching materials were conflicting, ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81988-6
更新日期：1991-08-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to investigate if nickel-titanium instruments with similar designs manufactured by different thermal treatments would exhibit significantly different in vitro behavior. METHODS:Thirty-six instruments each of ProTaper Universal (PTU F1; Dentsply Maillefer, Ballaigues, Switzerl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.06.005
更新日期：2015-01-01 00:00:00
abstract：INTRODUCTION:An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells. METHODS:A...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.09.006
更新日期：2019-01-01 00:00:00
abstract：:The aim of this study was to investigate fracture patterns occurring when a human upper central incisor is subjected to impact loadings at various angles. A two-dimensional finite element (FE) model of the maxillary incisor and surrounding tissues was established. The structural damping factor for the tooth was then c...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000157992.29221.41
更新日期：2005-10-01 00:00:00
abstract：:When dentin is planed by endodontic instruments, a smear layer forms. Whether this layer should be removed is unknown and controversial. This study was conducted to assess the effect of the smear layer on retention of bacteria using an in vitro root canal bacterial colonization model. Canals of 26 extracted human cani...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81186-6
更新日期：1994-02-01 00:00:00
abstract：INTRODUCTION:This study reports the antimicrobial effect of photodynamic therapy (PDT) combined with endodontic treatment in patients with necrotic pulp infected with microflora resistant to a previous antibiotic therapy. METHODS:Thirty anterior teeth from 21 patients with periapical lesions that had been treated with...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章
doi：10.1016/j.joen.2010.06.001
更新日期：2010-09-01 00:00:00
abstract：INTRODUCTION:The exact mechanisms of periapical bone resorption have not been fully elucidated. This study aimed to analyze the expression of Notch signaling molecules (Notch2, Jagged1, and Hey1) and proinflammatory cytokines (tumor necrosis factor alpha [TNF-α], interleukin [IL]-1β, and IL-6) in human apical periodont...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.10.015
更新日期：2019-02-01 00:00:00
abstract：:The purpose of treating furcal perforation is to seal the artificial communication between the endodontic space and the periradicular tissue to prevent alveolar bone resorption and damage to the periodontal ligament. These complications are not infrequent in cases of furcal and/or old perforations, which show a worse ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.05.019
更新日期：2008-09-01 00:00:00
abstract：INTRODUCTION:The purpose of this in-depth investigation was to identify, clarify, and substantiate clinical terminology relative to apical/periapical/periradicular diagnostic states, which is used routinely in the provision of endodontic care. Furthermore, the information gleaned from this investigation was used to lin...
journal_title：Journal of endodontics
pub_type： 共识发展会议,杂志文章
doi：10.1016/j.joen.2009.09.028
更新日期：2009-12-01 00:00:00
abstract：INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Prolif...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.05.035
更新日期：2011-09-01 00:00:00
abstract：:The objective of this study was to compare the sealing ability of ActiV GP/glass ionomer (GI) sealer (Brasseler USA, Savannah, GA), Resilon/Epiphany (Pentron Clinical Technologies, Wallingford, CT), and gutta-percha (GP)/AH Plus (Dentsply Maillefer, Tulsa, OK). Seventy-three human single-rooted teeth were randomly div...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.03.006
更新日期：2008-06-01 00:00:00
abstract：INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.11.002
更新日期：2018-03-01 00:00:00
abstract：:The sealing properties of three temporary restorative materials, Cavit, IRM, and a polycarboxylate-based cement, Ultratemp Firm, were investigated in vitro. Standardized access cavities were prepared in 45, intact, extracted, human molars. The teeth were randomly assigned to three groups and the access openings filled...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/01.don.0000121610.63000.f2
更新日期：2004-08-01 00:00:00
abstract：:Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.037
更新日期：2006-03-01 00:00:00
abstract：:The aim of the present study was to evaluate the in vivo antimicrobial activity of 2% chlorhexidine gluconate (FCFRP-USP) used as a root canal irrigating solution in teeth with pulp necrosis and radiographically visible chronic periapical reactions. Culture techniques and measurement of the inhibition zone were used. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(99)80135-6
更新日期：1999-03-01 00:00:00
abstract：:Use of resin-based restorative materials recently has become widely accepted for treatment of endodontically treated teeth. However, some solutions routinely used during endodontic treatment procedures may have an effect on bond strengths of adhesive materials to root canal dentin. The purpose of this in vitro study w...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/00004770-200402000-00013
更新日期：2004-02-01 00:00:00
abstract：INTRODUCTION:We evaluated the radiopacity and antibacterial properties of various concentrations of double antibiotic paste (DAP) containing barium sulfate (BaSO4) or zirconium oxide (ZrO2) radiopaque agents. METHODS:The radiopacity of 1, 10, and 25 mg/mL DAP containing 30% (w/v) BaSO4 or ZrO2, DAP-free radiopaque pas...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.06.010
更新日期：2018-09-01 00:00:00
