Abstract:
INTRODUCTION:Growth and differentiation factor-5 (GDF-5) is a multifunctional protein that regulates the development and repair in many tissues. The purpose of this study was to investigate whether GDF-5 may influence the proliferation, differentiation, and collagen turnover of human dental pulp cells. METHODS:Human dental pulp cells were treated with different concentrations of GDF-5 (0-500 ng/mL). Morphology of pulp cells was observed under a microscope. Cell proliferation was evaluated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. Immunofluorescent assay was used to observe the percentages of cell mitosis. Collagen content was measured by Sircol collagen assay. Tissue inhibitor of metalloproteinase-1 level in the culture medium was measured with enzyme-linked immunosorbent assay and Western blotting. Cell differentiation was evaluated by alkaline phosphatase (ALP) staining and ALP enzyme activity assay. RESULTS:After exposure of dental pulp cells to various concentrations of GDF-5, cell number was up-regulated significantly in dose-dependent manner. GDF-5 also stimulated mitosis of dental pulp cells as indicated by an increased percentage of binucleated cells from 28% to 35%-45%. GDF-5 did not affect the collagen content and tissue inhibitor of metalloproteinase-1 level of pulp cells. GDF-5 decreased the ALP activity of pulp cells as analyzed by ALP staining and enzyme activity assay, with 14%-44% of inhibition. CONCLUSIONS:GDF-5 revealed mitogenic and proliferative activity to dental pulp cells. GDF-5 showed inhibitory effect on ALP activity but little effect on the collagen turnover. These events are crucial in specific stages of dental pulp repair and regeneration. GDF-5 may be potentially used for tissue engineering of pulp-dentin complex.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Chang MC,Lin LD,Tseng HC,Chang BE,Chan CP,Lee SY,Chang HH,Lin PS,Tseng SK,Jeng JHdoi
10.1016/j.joen.2013.06.003subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1272-7issue
10eissn
0099-2399issn
1878-3554pii
S0099-2399(13)00540-2journal_volume
39pub_type
杂志文章abstract::Twenty volunteers with first molars free of restorations and caries were recruited. One molar from each arch was selected, and rubber dam was applied. Seven sites on each crown were electric pulp tested 4 times with an Elements Diagnostic Unit, and lowest threshold responses were recorded. Data were analyzed with one-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.08.006
更新日期:2007-11-01 00:00:00
abstract::Microbial control--fundamental to healing of apical periodontitis--is central to endodontic practice. The effectiveness of antibacterial measures is generally monitored (in clinical research studies) by microbiological root canal sampling (MRS), which is often used as a predictor for healing. This article addresses th...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2006.11.006
更新日期:2007-03-01 00:00:00
abstract::The purpose of this study was to determine the influence of operator experience on the ability to locate and fill extra canals in maxillary first and second molars in vivo. The number of canals located and filled from maxillary first and second molars was recorded from three endodontic residents during the first 6 mon...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.11.005
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Issues pertaining to the effective elimination of bacterial biofilm and disruption of biofilm structure still remains in endodontic disinfection. Nanoparticulates such as chitosan (CS-np) and zinc oxide (ZnO-np) are known to possess significant antibacterial properties. This study aimed to test (1) the eff...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.02.008
更新日期:2010-06-01 00:00:00
abstract:INTRODUCTION:Apical root resection is a biologically essential component in endodontic microsurgery. However, because it reduces the total root length and supported root surface, it changes the biomechanical response of the tooth. The purpose of this study was to analyze the biomechanical effect of apical root resectio...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.03.006
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:The inferior alveolar nerve (IAN) block does not always result in successful pulpal anesthesia. Anesthetic success rates might be affected by increased anxiety. Nitrous oxide has been shown to have both anxiolytic and analgesic properties. Therefore, the purpose of this prospective, randomized, double-blin...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2012.02.010
更新日期:2012-05-01 00:00:00
abstract:INTRODUCTION:Cell migration is an important step in pulpal wound healing. Although components in the resin-based dental materials are known to have adverse effects on pulp wound healing including proliferation and mineralization, their effects on cell migration have been scarcely examined. Here, we investigated the eff...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.06.004
更新日期:2013-09-01 00:00:00
abstract:INTRODUCTION:This clinical study evaluates the effect of ultrasonic root-end preparations on dentinal defect creation and propagation. METHODS:Eighty-four teeth were treated with periapical microsurgery using a modern microsurgical protocol in a private practice setting. The root apices were resected and inspected for...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.07.016
更新日期:2016-10-01 00:00:00
abstract::The current external inflammatory root resorption treatment protocol, which uses calcium hydroxide dressing, usually comprises multiple and long-term applications. In addition to the need for multiple appointments for calcium hydroxide replacement, the long-term maintenance of this compound in the root canal weakens d...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.03.019
更新日期:2015-09-01 00:00:00
abstract::Removing posts from root canals prior to endodontic retreatment is often a challenge. The Gonon post removing system (Thomas Extracteur De Pivots; FFDM-Pneumat, Bourges, France) is safe and efficient and can be used for anterior, bicuspid, and even molar teeth. The Gonon system is illustrated with one clinical case. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80200-6
更新日期:1989-11-01 00:00:00
abstract::The aim of this study was to measure root-dentin microhardness after instrumentation with two types of files and using irrigation with 2.5% sodium hypochlorite. Thirty roots were instrumented with irrigation: 10 roots had the pulp extirpated only; 10 roots were instrumented with stainless steel files, and 10 roots wer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200204000-00012
更新日期:2002-04-01 00:00:00
abstract:INTRODUCTION:In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesize...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.09.015
更新日期:2019-01-01 00:00:00
abstract::This in vitro study compared the accuracy of file length measurements made on calibrated and uncalibrated direct digital images. Endodontic files of known lengths and ISO sizes were used in 10 single-rooted, relatively straight teeth within cadaver specimens. The crowns of the teeth were ground flat and an orthodontic...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200112000-00017
更新日期:2001-12-01 00:00:00
abstract:INTRODUCTION:Periodontal mechanoreceptors (PMRs) are refined neural receptors present in abundance at the root apex and have a pivotal role in oral fine motor control. This case-control study aimed to evaluate the oral fine motor control of teeth treated with endodontic microsurgery (EMS) in comparison with the control...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2020.10.022
更新日期:2021-02-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare centering ability and apical canal transportation of K-files (KF), ProGlider (PG), and G-Files (GF) after glide path enlargement in curved canals by using micro-computed tomography. METHODS:Thirty canals each were randomly assigned to KF, GF, and PG. Teeth were sca...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.04.005
更新日期:2016-07-01 00:00:00
abstract::Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from h...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200203000-00003
更新日期:2002-03-01 00:00:00
abstract:INTRODUCTION:Cone-beam computed tomographic (CBCT) imaging is a valuable tool for endodontic therapy. The aim of this study was to verify whether clinical use of CBCT imaging can accurately acquire parameters concerning molar pulp chamber landmarks, which are important data to help start a successful access cavity and ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.04.002
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:This study aimed to compare the success rates of direct pulp capping (DPC) by using either ProRoot Mineral Trioxide Aggregate (MTA) or Biodentine in the cariously exposed permanent teeth of 6- to 18-year-old patients. Gray discoloration was also evaluated. METHODS:Fifty-nine cariously exposed permanent te...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2017.10.012
更新日期:2018-03-01 00:00:00
abstract:INTRODUCTION:Root canal therapy is a highly successful in-office treatment and preventive measure against periapical abscesses. Left untreated, periapical abscesses can have serious consequences that can lead to hospitalization. This study observes the trends of hospitalizations attributed to periapical abscesses. MET...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.04.042
更新日期:2013-09-01 00:00:00
abstract::The inferior alveolar nerve is sometimes affected by periapical pathoses and mandibular cysts. However, mandibular intraosseous lesions have not been reported to disturb the lingual nerve. A case of simultaneous paresthesia of the right lingual nerve and the right inferior alveolar nerve is presented. The possible mec...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000158011.64829.7b
更新日期:2005-10-01 00:00:00
abstract::The purpose of this study was to evaluate the strengthening effect of an internal resin bonding technique. One hundred central incisors were endodontically treated and divided equally into five groups. Group 1, without any cervical preparation and with access repaired with bonded resin, served as the positive control....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(98)80108-8
更新日期:1998-04-01 00:00:00
abstract:INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Prolif...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.05.035
更新日期:2011-09-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to determine factors and clinical situations that influence an endodontist's decision to use guided tissue regeneration (GTR) techniques during endodontic root-end surgery. METHODS:An invitation to participate in a web-based survey was e-mailed to 3,750 members of the America...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.08.017
更新日期:2011-11-01 00:00:00
abstract:INTRODUCTION:This study evaluated the success rate of and tooth substance removal required for computer-guided preparation of endodontic access cavities. METHODS:Thirty acrylic typodont teeth with root canals (10 each of tooth numbers 11, 14, and 17) were randomly allocated to a study or control group (15 teeth per gr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.10.025
更新日期:2021-02-01 00:00:00
abstract::Ten extracted human maxillary first molars were selected with a variety of root curvatures. The distofacial roots were resected and the mesiofacial canals were instrumented to a size #25 K file. Three techniques were used to introduce Ca(OH)2 paste into the instrumented canals. These techniques were evaluated for thei...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)81220-3
更新日期:1992-08-01 00:00:00
abstract:INTRODUCTION:This study evaluated the biocompatibility of 5 and 10 μg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. METHODS:Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, an...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.08.010
更新日期:2017-12-01 00:00:00
abstract::Various classes of immunoglobulins have been found in human periapical lesions. The specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. The purpose of this study was to test the specificity of antibodies present in human periapical lesions. Hu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81923-0
更新日期:1991-05-01 00:00:00
abstract::The purpose of this study was to evaluate the integrity of resected root-end surfaces of extracted human teeth after using ultrasonic tips at low or high frequencies for root-end preparation. Thirty bilaterally matched pairs of single-rooted human teeth had root-end resections using a low-speed diamond saw and were ex...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80091-4
更新日期:1996-04-01 00:00:00
abstract::Sixty-eight standardized human root specimens were infected with Enterococcus faecalis for 3 wk after removal of the smear layer. After 3 wk of infection, the smear layer was reformed and in half of the specimens, the smear layer was again removed. Aqueous Ca(OH)2 paste and silicone oil based Ca(OH)2 paste were used a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200105000-00004
更新日期:2001-05-01 00:00:00
abstract::The purpose of this study was to compare three engine driven (nickel-titanium) NiTi instrument systems with hand files for their effect on canal transportation. Mesial roots of mature lower first molars with separate canals were paired on the basis of curvature and morphology. Canal lengths were standardized to 11 mm ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80310-X
更新日期:1997-08-01 00:00:00