Abstract:
INTRODUCTION:It has been widely accepted that dental pulp stem cells (DPSCs), which are a class of self-renewal and differentiation potential of adult stem cells, play an important role in the repair procession of pulp's inflammation. We investigated whether tumor necrosis factor alpha (TNF-α) could induce the proliferation of DPSCs and clarified the potential mechanism of this proliferation. METHODS:Cell Counting Kit-8 assay (Dojindo Laboratories, Mashiki-machi, Kumamoto, Japan) and 5-ethynyl-2'-deoxyuridine-based proliferation assays were determined to investigate various concentrations or hours of TNF-α inducing a cell number change of DPSCs. Next, flow cytometry analysis was performed to investigate the main cell cycle phase process of DPSCs. Furthermore, the signaling pathway of TNF-α-induced proliferation of DPSCs was analyzed using Western blot analysis. Then, inhibitors were added to confirm the mechanism of this signaling pathway. RESULTS:TNF-α induced the proliferation of DPSCs in a dose- and time-dependent manner. Cyclin D1, which controlled the cell cycle process from the G1 to the S phase, was up-regulated by TNF-α in a time-dependent manner, whereas its overexpression alone increased DPSC proliferation. Furthermore, TNF-α was capable of inducing Akt/GSK-3β signaling pathway activation. Blockage of phosphoinositide 3-kinase/Akt by their kinase or genetic inhibitors could significantly reduce TNF-α-induced proliferation of DPSCs. CONCLUSIONS:This study confirmed that TNF-α induced the proliferation of DPSCs by regulating the Akt/GSK-3β/cyclin D1 signaling pathway and then provided a suitable number for the requirements of cell differentiation.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Qin Z,Li Y,Li Y,Liu Gdoi
10.1016/j.joen.2015.02.020subject
Has Abstractpub_date
2015-07-01 00:00:00pages
1066-72issue
7eissn
0099-2399issn
1878-3554pii
S0099-2399(15)00180-6journal_volume
41pub_type
杂志文章abstract::The cyclic fatigue resistance of three types of nickel-titanium rotary files was compared in a model using reciprocating axial movement. The influence of file size and taper was also investigated and fracture patterns were examined under SEM. The 10 experimental groups consisted of ProFiles, K3s, and RaCe files, size ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.013
更新日期:2006-01-01 00:00:00
abstract::Various cells and inflammatory mediators are involved in the initial pulpal responses to caries. This review focuses on the cellular, neuronal, and vascular components of pulpal innate responses to caries. Discussion will include dentinal fluid, odontoblasts, neuropeptides, and neurogenic inflammation, which are not c...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2007.01.001
更新日期:2007-06-01 00:00:00
abstract::Various classes of immunoglobulins have been found in human periapical lesions. The specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. The purpose of this study was to test the specificity of antibodies present in human periapical lesions. Hu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81923-0
更新日期:1991-05-01 00:00:00
abstract::A molar-incisor malformation (MIM) is a newly reported type of dental anomaly that involves a characteristic root malformation in permanent first molars and deciduous second molars and a crown defect in permanent central incisors. This case report describes a nonsurgical root canal treatment of a MIM-affected molar by...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.11.004
更新日期:2016-04-01 00:00:00
abstract::This case-control study sought to identify variables predictive of subsequent root canal therapy (RCT) in teeth receiving full coverage restorations. The University of North Carolina School of Dentistry's computerized treatment database was used to identify all patients receiving a single-unit crown on a nonendodontic...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.11.001
更新日期:2006-08-01 00:00:00
abstract::The purpose of this prospective, double-blind, randomized study was to evaluate pain reduction using an intraosseous injection of slow-releasing methylprednisolone in teeth with irreversible pulpitis. Forty subjects presenting for emergency treatment completed the study. Each subject had a tooth with a clinical diagno...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200011000-00001
更新日期:2000-11-01 00:00:00
abstract:INTRODUCTION:The pulpal anesthetic success rates for an inferior alveolar nerve block (IANB) alone in patients presenting with symptomatic irreversible pulpitis are less than adequate. Nitrous oxide and clonidine have shown increases in IANB success when administered individually, but their success has not been evaluat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.12.014
更新日期:2020-12-30 00:00:00
abstract:INTRODUCTION:Compelling evidence pinpoints that pulp tissue engineering after the transplantation of stem cells is possible. Although intriguing, severe problems regarding clinical feasibility remain. Cell homing has been proposed as a viable alternative in which dentin-derived growth factors in a conducive scaffold ma...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.02.011
更新日期:2018-06-01 00:00:00
abstract::The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.08.001
更新日期:2006-04-01 00:00:00
abstract::Fusion and gemination are developmental anomalies of the dental hard tissues that might require endodontic and surgical treatment for functional, orthodontic, or esthetic reasons. In this article, we report a rare case of successful endodontic management of unilateral fused mandibular second molar with a paramolar. Th...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.06.021
更新日期:2007-10-01 00:00:00
abstract:INTRODUCTION:Neuropeptides play an important role in inflammation and repair and have been implicated in mediating angiogenesis. Pulp fibroblasts express neuropeptide receptors, and the aim of this research was to investigate whether neuropeptides could regulate angiogenic growth factor expression in vitro METHODS:An ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.03.005
更新日期:2009-06-01 00:00:00
abstract::The purpose of this study was to evaluate gray mineral trioxide aggregate (MTA), white MTA, and Fuji II LC cement as coronal barriers to bacterial leakage. Seventy-eight, matched, human teeth were obturated with gutta-percha. In group I, 18 pairs received a 3-mm barrier of gray or white MTA. In group II, 18 pairs rece...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200411000-00008
更新日期:2004-11-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the accuracy of cone-beam computed tomographic (CBCT) to measure dentin thickness and its potential of predicting the remaining dentin thickness after the removal of fractured instrument fragments. METHODS:Twenty-three human mandibular molars were selected, and 4-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.03.041
更新日期:2017-09-01 00:00:00
abstract::Increased levels of interleukin-1 (IL)-1, tumor necrosis factor-alpha (TNF-alpha), IL-6, and prostaglandin E2 (PGE2) have been detected in inflamed pulp tissue. To gain further insight into the molecular pathogenesis of pulpitis, we investigated the effects of IL-1alpha or TNF-alpha and PGE2, either alone or in combin...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200203000-00013
更新日期:2002-03-01 00:00:00
abstract::The expression of the vanilloid receptor subtype 1 (VR1, TRPV1) was detected in human dental pulp fibroblasts (PF-10) using RT-PCR, Western blotting, and immunocytochemical analysis. As revealed by ELISA, capsaicin induced IL-6 expression in PF-10 cells, and the VR1 antagonist capsazepine dose-dependently inhibited ca...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000155259.22746.ae
更新日期:2005-09-01 00:00:00
abstract::Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200306000-00011
更新日期:2003-06-01 00:00:00
abstract:INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.001
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to investigate the release of growth factors into root canal space after the irrigation procedure of regenerative endodontic procedure. METHODS:Sixty standardized root segments were prepared from extracted single-root teeth. Nail varnish was applied to all surfaces except the roo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.04.029
更新日期:2016-12-01 00:00:00
abstract::A descriptive, cross sectional survey was developed to determine the preferences of endodontists when prescribing analgesics. Eleven clinical scenarios describing common endodontic diagnoses or procedures with specified severity of pain were provided. A survey was sent to 310 AAE members and 63 responded, providing a ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.07.015
更新日期:2006-12-01 00:00:00
abstract::The cytotoxicity against two mesenchymal cell lines, L-929 and WI-38, and two epithelial cell lines, KB and HeLa S-3, of lipopolysaccharide (LPS) purified from strains of Bacteroides gingivalis, Fusobacterium nucleatum, and Veillonella parvula isolated from infected root canals was investigated. The inhibition of cell...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80195-5
更新日期:1989-11-01 00:00:00
abstract:INTRODUCTION:Many studies have investigated the morphology of the mandibular molar, but the prevalence of the middle mesial (MM) canal in the mesial root of the mandibular molar is still the subject of controversy. In addition, in previous literature, a true MM canal has not been clearly distinguished from an isthmus b...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.02.008
更新日期:2017-07-01 00:00:00
abstract:INTRODUCTION:This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts. METHODS:Biodentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 d...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.11.026
更新日期:2013-04-01 00:00:00
abstract::This study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. Microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. The canal contents were analyzed by aerobic/an...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000148149.27636.9c
更新日期:2005-06-01 00:00:00
abstract::The antimicrobial effect of RC-Prep and its components--10% urea peroxide, 15% EDTA, and glycol--were evaluated individually, in vitro, within dentinal tubules. Standardized bovine incisor root specimens that had been infected with either Staphylococcus aureus or Streptococcus peltzer were incubated with each agent fo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80296-9
更新日期:1999-12-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the influence of pH that is due to setting reaction of Biodentine, glass ionomer cement (GIC), and intermediate restorative material (IRM) on transforming growth factor-β1 (TGF-β1) release and on the fibrin architecture of platelet-rich fibrin (PRF). METHODS:PRF w...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.02.009
更新日期:2016-05-01 00:00:00
abstract::The purpose of this study was to evaluate the number of canals and isthmuses obturated after Cr, Er: YSGG laser treatment. Fifty-two canals were instrumented to a size 40 Profile 0.06 file combined with RC-Prep and sodium hypochlorite, or sterile water. The teeth were divided into 3 groups: (I) canals were flooded wit...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2007.05.012
更新日期:2007-09-01 00:00:00
abstract::The purpose of this study was to determine the influence of operator experience on the ability to locate and fill extra canals in maxillary first and second molars in vivo. The number of canals located and filled from maxillary first and second molars was recorded from three endodontic residents during the first 6 mon...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.11.005
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:It has been reported that integrin-α5 (ITGA5) activity is related to cell proliferation, differentiation, migration, and organ development. However, the involvement of ITGA5 in the biological functions of human dental pulp stem cells (hDPSCs) has not been explored. The aim of this study was to investigate ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.011
更新日期:2014-02-01 00:00:00
abstract::The purpose of this survey was to investigate the frequency and characteristics of surgery among active endodontists in the United States. The survey also inquired about residency and postresidency training in endodontic surgery. A Web-based questionnaire regarding endodontic surgery was distributed via e-mail to 2,53...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.10.008
更新日期:2009-01-01 00:00:00
abstract:INTRODUCTION:An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells. METHODS:A...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.09.006
更新日期:2019-01-01 00:00:00