Abstract：
INTRODUCTION:An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells. METHODS:AH Plus (Dentsply Caulk, Milford, DE), MTA Fillapex (Angelus Solucxoes Odontologicas, Londrina, Brazil), and EndoSequence BC (Brasseler, Savannah, GA) were mixed according to the manufacturer's instructions, and samples were prepared as extraction media (final dilution: 1/10). Lipopolysaccharide (LPS) (100 ng/mL) treatment was used to induce an inflammatory response in this study. Cell viability was evaluated using the Water soluble tetrazolium-1 (WST-1) assay. The levels of inflammatory mediators (interleukin 6 and tumor necrosis factor alpha) and osteogenic marker genes (ALP and OCN) were measured by reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. Osteogenic potential was evaluated by alkaline phosphatase staining and alizarin red staining. RESULTS:Calcium silicate-based sealers such as MTA Fillapex and EndoSequence BC showed strong cell viability compared with AH Plus. AH Plus, MTA Fillapex, and EndoSequence BC decreased the levels of LPS-induced inflammatory mediators (P < .05). The expression of osteogenic marker genes, alkaline phosphatase activity, and mineralized nodule formation decreased with LPS treatment. However, AH Plus and calcium silicate-based sealers increased the osteogenic potential reduced by LPS treatment (P < .05). CONCLUSIONS:Calcium silicate-based sealers exhibit anti-inflammatory effects and induce osteogenic differentiation in MC3T3-E1 cells.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Lee BN,Hong JU,Kim SM,Jang JH,Chang HS,Hwang YC,Hwang IN,Oh WMdoi
10.1016/j.joen.2018.09.006subject
Has Abstractpub_date
2019-01-01 00:00:00pages
73-78issue
1eissn
0099-2399issn
1878-3554pii
S0099-2399(18)30617-4journal_volume
45pub_type
杂志文章abstract：:When dentin is planed by endodontic instruments, a smear layer forms. Whether this layer should be removed is unknown and controversial. This study was conducted to assess the effect of the smear layer on retention of bacteria using an in vitro root canal bacterial colonization model. Canals of 26 extracted human cani...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81186-6
更新日期：1994-02-01 00:00:00
abstract：INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.11.002
更新日期：2018-03-01 00:00:00
abstract：:This study compares the incidence, degree, and length of postoperative pain in 300 endodontically treated teeth, with and without apical patency, in relation to some diagnostic factors (vitality, presence of preoperative pain, group, and mandible of treated tooth). Of the questionnaires received back, apical patency w...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2008.11.014
更新日期：2009-02-01 00:00:00
abstract：INTRODUCTION:The introduction of piezoelectric instruments in endodontic surgery allowed clinicians to manage the bone tissue with precision reducing the risk of damaging soft tissues. Although such instruments can also be used to prepare root-end cavity, few information is available on the effect of piezosurgery on de...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.028
更新日期：2010-10-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate and compare the resistance to cyclic fatigue of the new One Curve (OC) instrument in double (S-shaped) and single curvature canals compared with other nickel-titanium rotary instruments. METHODS:Size 25/.06 of OC, 2Shape (TS), Vortex Blue (VB), ProFile Vortex (PV), an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.023
更新日期：2018-11-01 00:00:00
abstract：INTRODUCTION:Pain present 6 months after root canal treatment (RCT) may be of odontogenic or nonodontogenic origin. This is important because treatments and prognoses are different; therefore, the aim of this study was to provide specific diagnoses of patients reporting pain 6 months after receiving initial orthograde ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.12.012
更新日期：2015-04-01 00:00:00
abstract：INTRODUCTION:Previous studies have suggested that masticatory forces are associated with cracked teeth, but it is not clear which specific poor oral masticatory habits may be more likely to cause cracks. The aim of this study was to quantitatively assess risk factors for cracked teeth among poor oral masticatory habits...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.01.007
更新日期：2017-06-01 00:00:00
abstract：:The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tet...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000152899.54247.4e
更新日期：2005-07-01 00:00:00
abstract：:Osteoradionecrosis (ORN) of the jaw is considered the most severe long-term adverse effect that can occur in radiation therapy patients. Teeth extractions and dental diseases are the main risk factors for ORN in irradiated patients. The aim of this case series was to evaluate the outcome of primary root canal treatmen...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.12.005
更新日期：2020-04-01 00:00:00
abstract：INTRODUCTION:Calcium hydroxide (Ca[OH]2) is a widely used interappointment dressing, but its antibacterial property is compromised by dentin. Hence, the addition of chlorhexidine (CHX) with Ca(OH)2 has been proposed. However, the antimicrobial efficacy of this mixture compared with Ca(OH)2 alone is currently still deba...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.01.020
更新日期：2016-05-01 00:00:00
abstract：:Primary intraosseous salivary gland tumors are rare, with mucopidermoid carcinoma being the most frequent histotype. The authors present a case of adenoid cystic carcinoma, located in the mandibular incisor region, associated with pain. Endodontic treatment resulted in increased pain and progressive mandibular expansi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200012000-00026
更新日期：2000-12-01 00:00:00
abstract：:Use of resin-based restorative materials recently has become widely accepted for treatment of endodontically treated teeth. However, some solutions routinely used during endodontic treatment procedures may have an effect on bond strengths of adhesive materials to root canal dentin. The purpose of this in vitro study w...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/00004770-200402000-00013
更新日期：2004-02-01 00:00:00
abstract：:Locating the number and position of orifices on pulp-chamber floors can be difficult. This is especially true when the tooth being treated is heavily restored, malposed, or calcified. After evaluating 500 pulp chambers of extracted teeth, new laws for finding pulp chambers and root-canal orifices are proposed. The use...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200401000-00002
更新日期：2004-01-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to analyze and characterize root canal morphology of mandibular molars of the Brazilian population by using cone-beam computed tomography (CBCT). METHODS:Patients who required CBCT radiographic examinations as part of their routine examination, diagnosis, and treatment planning w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.030
更新日期：2013-07-01 00:00:00
abstract：INTRODUCTION:Many studies have investigated the morphology of the mandibular molar, but the prevalence of the middle mesial (MM) canal in the mesial root of the mandibular molar is still the subject of controversy. In addition, in previous literature, a true MM canal has not been clearly distinguished from an isthmus b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.008
更新日期：2017-07-01 00:00:00
abstract：:The sealing properties of three temporary restorative materials, Cavit, IRM, and a polycarboxylate-based cement, Ultratemp Firm, were investigated in vitro. Standardized access cavities were prepared in 45, intact, extracted, human molars. The teeth were randomly assigned to three groups and the access openings filled...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/01.don.0000121610.63000.f2
更新日期：2004-08-01 00:00:00
abstract：INTRODUCTION:Osteoporosis is a major systemic disease that can significantly deteriorate the quality of life of the affected individuals. It is more common in women, particularly after menopause. Osteoporosis may be associated with alterations in oral health. Treatment of osteoporotic patients mainly involves the admin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.10.019
更新日期：2021-02-01 00:00:00
abstract：:Previous studies have indicated that vertical root fracture tends to occur in a buccolingual direction, where dentin thickness is greatest. Factors potentially influencing the location and direction of root fracture include root canal shape, external root morphology, and dentin thickness. In this finite-element study,...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200308000-00008
更新日期：2003-08-01 00:00:00
abstract：:This study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. Microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. The canal contents were analyzed by aerobic/an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000148149.27636.9c
更新日期：2005-06-01 00:00:00
abstract：:The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.08.001
更新日期：2006-04-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to visualize the stresses and strain distribution patterns in ProTaper Universal F2 files (Dentsply Maillefer, Ballaigues, Switzerland) and to establish the stress- and strain-curvature relationship for this instrument under various conditions by using a dynamic, three-dimensi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.06.003
更新日期：2011-10-01 00:00:00
abstract：:The purpose of this study was to examine the effect of the smear layer on apical microleakage over 16 wk. One hundred and four extracted human teeth were assigned to four groups treated as follows: group A1--smear layer was left intact and canals were obturated with gutta-percha and Roth 811; group A2--smear layer was...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80010-7
更新日期：1999-02-01 00:00:00
abstract：:The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37 degrees C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and acces...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200007000-00001
更新日期：2000-07-01 00:00:00
abstract：INTRODUCTION:We evaluated the radiopacity and antibacterial properties of various concentrations of double antibiotic paste (DAP) containing barium sulfate (BaSO4) or zirconium oxide (ZrO2) radiopaque agents. METHODS:The radiopacity of 1, 10, and 25 mg/mL DAP containing 30% (w/v) BaSO4 or ZrO2, DAP-free radiopaque pas...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.06.010
更新日期：2018-09-01 00:00:00
abstract：:The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.02.009
更新日期：2018-05-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the incidence and mode of WaveOne (Dentsply Tulsa Dental Specialties, Tulsa, OK) instrument defects after single use at different endodontic clinics. METHODS:A total of 438 WaveOne instruments were collected after clinical use from the 4 specialist clinics over a ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.10.008
更新日期：2016-02-01 00:00:00
abstract：:No information could be found in the dental literature on the hardness or strength of the materials in root canal instruments. Hardness testing is a convenient method for examining these products, and strength properties can be deduced from available strength-hardness data. The Vickers hardness of cross-sections of st...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80274-3
更新日期：1996-02-01 00:00:00
abstract：:Periapical lesions usually are caused by root canal infection; nevertheless, other pathologies may eventually involve the tooth apex, making the correct diagnosis more difficult. Glandular odontogenic cysts (GOCs) are uncommon and, despite their cystic nature, may present an aggressive behavior and a high recurrence r...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.06.015
更新日期：2017-11-01 00:00:00
abstract：:The purpose of this study was to evaluate a new periapical index based on cone beam computed tomography (CBCT) for identification of apical periodontitis (AP). The periapical index proposed in this study (CBCTPAI) was developed on the basis of criteria established from measurements corresponding to periapical radioluc...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.013
更新日期：2008-11-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to assess temperature changes in conventional gutta-percha, thermoplastic gutta-percha, and Resilon cones by using thermomechanical compaction technique. METHODS:The root canals of extracted human teeth were placed in resin blocks and sectioned. After root canal preparation, cavi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.03.009
更新日期：2009-06-01 00:00:00
