Abstract：
:When dentin is planed by endodontic instruments, a smear layer forms. Whether this layer should be removed is unknown and controversial. This study was conducted to assess the effect of the smear layer on retention of bacteria using an in vitro root canal bacterial colonization model. Canals of 26 extracted human canines were step-back prepared using 2.5% NaOCl. Teeth were then randomly divided into two groups based on the type of high volume final flush: 1-20 ml of sterile saline (0.85% wt/vol) or (2-10 ml of 17% EDTA followed by 10 ml of 2.5% NaOCl which removes smear layer. Streptococcus anginosus (milleri) was cultured in trypticase soy broth supplemented with 0.5% yeast extract at 37 degrees C in 5% CO2. Cells were harvested by centrifugation and resuspended in fresh media. Serial dilutions were performed to achieve inocula of 10(6) colony-forming units in a 30-microliters volume. Teeth were inoculated and incubated for 2 h in 5% CO2 at 37 degrees C. Following incubation, teeth were split and processed for microbiological analysis. Numbers of colonizing bacteria were determined by a spiral-plating system. Enumeration of the numbers of bacteria revealed a reproducible, order of magnitude difference (p = 0.0002) between teeth with smear layer (10(4) colony-forming units) versus teeth without smear layer (10(5) colony-forming units). This suggests that smear layer produced during root canal therapy may inhibit bacterial colonization of root canals. One suggested mechanism is that smear layer may block bacterial entry into dentinal tubules.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Drake DR,Wiemann AH,Rivera EM,Walton REdoi
10.1016/S0099-2399(06)81186-6subject
Has Abstractpub_date
1994-02-01 00:00:00pages
78-82issue
2eissn
0099-2399issn
1878-3554pii
S0099-2399(06)81186-6journal_volume
20pub_type
杂志文章abstract：:The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.08.001
更新日期：2006-04-01 00:00:00
abstract：:Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80268-4
更新日期：1999-06-01 00:00:00
abstract：INTRODUCTION:Previous studies in patients with irreversible pulpitis have reported increased success of the inferior alveolar nerve block (IANB) using premedication with ketorolac. Preemptive nitrous oxide administration has also shown an increase in the success of the IANB. Recently, ketorolac has been made available ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.09.011
更新日期：2018-01-01 00:00:00
abstract：:Dental pulp is a soft mesenchymal tissue densely innervated by afferent (sensory) fibers, sympathetic fibers, and parasympathetic fibers. This complexity in pulp innervation has motivated numerous investigations regarding how these 3 major neuronal systems regulate pulp physiology and pathology. Most of this research ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2008.03.010
更新日期：2008-07-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate and compare the resistance to cyclic fatigue of the new One Curve (OC) instrument in double (S-shaped) and single curvature canals compared with other nickel-titanium rotary instruments. METHODS:Size 25/.06 of OC, 2Shape (TS), Vortex Blue (VB), ProFile Vortex (PV), an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.023
更新日期：2018-11-01 00:00:00
abstract：INTRODUCTION:The introduction of piezoelectric instruments in endodontic surgery allowed clinicians to manage the bone tissue with precision reducing the risk of damaging soft tissues. Although such instruments can also be used to prepare root-end cavity, few information is available on the effect of piezosurgery on de...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.028
更新日期：2010-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate and compare, clinically and radiographically, the effects of platelet-rich fibrin (PRF) and mineral trioxide aggregate (MTA) as pulpotomy agents in permanent teeth with incomplete root development. METHODS:A total number of 70 children requiring pulpotomy in 70 pe...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2014.01.009
更新日期：2014-05-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the incidence and mode of WaveOne (Dentsply Tulsa Dental Specialties, Tulsa, OK) instrument defects after single use at different endodontic clinics. METHODS:A total of 438 WaveOne instruments were collected after clinical use from the 4 specialist clinics over a ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.10.008
更新日期：2016-02-01 00:00:00
abstract：:Etch-and-rinse adhesives can cause vasorelaxation via mechanisms occurring in the endothelium and smooth muscle, including the release of nitric oxide (NO). This effect might promote or aggravate bleeding if such adhesives are placed inadvertently on iatrogenic pulp microexposures. The present study assessed the vasoa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.07.019
更新日期：2008-10-01 00:00:00
abstract：:Two electronic apex locators based on the gradient (Apit) and absolute (Odontometer) impedance principles were evaluated. Distances of file tips to apical foramina were determined in vivo with the electronic apex locators and subsequently verified after extraction. Mean differences between file tips and apical foramin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80145-7
更新日期：1996-05-01 00:00:00
abstract：:The purpose of this study was to compare the in vitro sealing capacity of five materials, each used as a temporary sealing agent for the walking bleach technique. All teeth received traditional biomechanical root canal instrumentation, after which the walking bleach agent was placed in the pulp chamber space. The occl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200012000-00011
更新日期：2000-12-01 00:00:00
abstract：:We immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. Forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. The bacteria in the spe...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200303000-00008
更新日期：2003-03-01 00:00:00
abstract：:Microbial control--fundamental to healing of apical periodontitis--is central to endodontic practice. The effectiveness of antibacterial measures is generally monitored (in clinical research studies) by microbiological root canal sampling (MRS), which is often used as a predictor for healing. This article addresses th...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2006.11.006
更新日期：2007-03-01 00:00:00
abstract：INTRODUCTION:Revascularization is a valuable treatment in immature necrotic teeth that allows the continuation of root development. In this article we describe successful revascularization treatment of 2 necrotic immature first mandibular molars. METHODS:The clinical and radiographic examinations showed extensive coro...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2011.01.011
更新日期：2011-04-01 00:00:00
abstract：INTRODUCTION:Outcome studies of endodontic treatment of necrotic immature permanent teeth rely on radiographic measures as surrogates of whether the treatment achieved regeneration/revascularization/revitalization. An increase in radiographic root length and/or width is thought to result in a better long-term prognosis...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.09.025
更新日期：2014-01-01 00:00:00
abstract：INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.02.028
更新日期：2014-10-01 00:00:00
abstract：:A case is presented in which a previous partial root canal treatment on a mandibular molar developed internal resorption of the distal canal. The case was followed at the University of Oklahoma student clinic and endodontic treatment completed in the mesial canals, with calcium hydroxide placed in the distal canal for...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200107000-00010
更新日期：2001-07-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to investigate if nickel-titanium instruments with similar designs manufactured by different thermal treatments would exhibit significantly different in vitro behavior. METHODS:Thirty-six instruments each of ProTaper Universal (PTU F1; Dentsply Maillefer, Ballaigues, Switzerl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.06.005
更新日期：2015-01-01 00:00:00
abstract：:The objective of this study was to assess and compare canal blockages and apical extrusion of dentin debris during canal shaping with eight preparation techniques. A total of 208 canals in extracted human teeth were prepared by one operator using one type of file. The techniques included were standardized, stepback wi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80443-7
更新日期：1995-03-01 00:00:00
abstract：INTRODUCTION:Newer antibacterial alternatives such as chitosan nanoparticles (CSnps) and photodynamic therapy (PDT) have been investigated to achieve effective root canal disinfection. The current study aims to assess the effect of various tissue inhibitors such as dentin, dentin matrix, pulp tissue, bacterial lipopoly...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.05.006
更新日期：2012-09-01 00:00:00
abstract：INTRODUCTION:Regenerative endodontic procedures are an alternative treatment for immature teeth with necrotic pulps. Typically, intracanal medicaments such as triple antibiotic paste (TAP) or double antibiotic paste (DAP) and calcium hydroxide (Ca[OH](2)) are used for disinfection. However, their effect on human stem c...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.06.018
更新日期：2012-10-01 00:00:00
abstract：:The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.11.007
更新日期：2008-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to assess temperature changes in conventional gutta-percha, thermoplastic gutta-percha, and Resilon cones by using thermomechanical compaction technique. METHODS:The root canals of extracted human teeth were placed in resin blocks and sectioned. After root canal preparation, cavi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.03.009
更新日期：2009-06-01 00:00:00
abstract：:There have been major recent reorganizations among bacterial taxa as a result of phylogenetic taxonomic approaches. As a consequence, old species have been renamed and novel species have been proposed. The introduction of molecular technology for microbial identification has also allowed the detection of microbial tax...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/00004770-200310000-00001
更新日期：2003-10-01 00:00:00
abstract：:This study investigated the retreatment effectiveness of .04 Taper nickel-titanium rotary ProFiles. Thirty extracted single-rooted anterior teeth were instrumented and obturated with gutta-percha/Roth's Sealer using lateral condensation. They were distributed into three groups of 10 each. Retreatment for group A was d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200002000-00010
更新日期：2000-02-01 00:00:00
abstract：INTRODUCTION:Calcium silicate-based materials (CSMs) are used in various endodontic procedures. The present study examined whether prolonged contact of mineralized dentin with recently commercialized versions of these materials adversely affects dentin collagen matrix integrity. METHODS:Dentin slabs prepared from extr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.01.004
更新日期：2012-06-01 00:00:00
abstract：:Type IV matrix metalloproteinases (MMPs) are members of the family of MMPs and are thought to play an important role in degradation of extracellular components. Human pulp cells can secrete and produce these enzymes. Recent evidence shows that MMPs may play a role in pulpal inflammation. To date little is known regard...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200111000-00007
更新日期：2001-11-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the effects of pretreatment of gel chlorhexidine and ethanol on the bond strength and durability of the adhesion of the fiber post relined with resin composite to the root dentin. METHODS:Sixty bovine incisor roots were divided into four groups: irrigation with phy...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.01.007
更新日期：2011-05-01 00:00:00
abstract：INTRODUCTION:This study evaluated the risk of failure for an endodontically treated premolar with different crack depths, which was shearing toward the pulp chamber and was restored by using 3 different computer-aided design/computer-aided manufacturing ceramic restoration configurations. METHODS:Three 3-dimensional f...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.11.042
更新日期：2013-03-01 00:00:00
abstract：:A method is proposed for a three-dimensional visualization of the root canal system before and after the cleaning and shaping procedure to analyze the relationships of the root canal walls. The first part of the study provided cross-sections of the root perpendicular to the root canal. A system for remounting the sect...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81298-7
更新日期：1993-05-01 00:00:00
