当前位置： SCI文献检索 > JOURNAL OF ENDODONTICS期刊下所有文献 > Regulation of matrix metalloproteinase-2 production by cytokines and pharmacological agents in human pulp cell cultures.

Regulation of matrix metalloproteinase-2 production by cytokines and pharmacological agents in human pulp cell cultures.

Abstract：

:Type IV matrix metalloproteinases (MMPs) are members of the family of MMPs and are thought to play an important role in degradation of extracellular components. Human pulp cells can secrete and produce these enzymes. Recent evidence shows that MMPs may play a role in pulpal inflammation. To date little is known regarding the regulation of MMPs in human pulp cell cultures. The purpose of this study was to determine the effects of cytokines (interleukin-1 and transforming growth factor-beta (TGF-beta), protein synthesis inhibitor cycloheximide (CD), and protein kinase C inhibitors (H7 and Go6976) on the secretion and production of MMPs by human pulp cell cultures using gelatin zymography. The main gelatinase secreted by human pulp cells migrated at 72 kDa and represented MMP-2. Minor gelatinolytic bands were also observed at 92 kDa regions that correspond to MMP-9. After an 8-day culture period TGF-beta, CD, H7, and Go6976 were found to depress MMP-2 production. The inhibition decreased in an order of CD > H7 > TGF-beta > Go6976. IL-1 was found to elevate MMP-2 production. Human pulp cells, however treated with either cytokines or pharmacological agents had no effect on the pattern of MMP-9 produced or secreted in either cell extracts or conditioned medium fractions. These observations suggest that the cytokines and pharmacological agents can regulate MMP-2 produced by human pulp cells. Inflammatory cytokines stimulate the production of elevated levels of MMP-2 and MMP-2 might play a role in pulpal inflammation. In addition agents that target protein synthesis or the protein kinase C pathway in human pulp cells inhibit MMP-2 production, and such inhibition may contribute to the pathogenesis of pulpal inflammation. Such inhibition might contribute to therapeutic efficacy.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Chang YC,Yang SF,Hsieh YS

doi

10.1097/00004770-200111000-00007

keywords:

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

679-82

issue

11

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)60483-9

journal_volume

27

pub_type

杂志文章

相关文献

JOURNAL OF ENDODONTICS文献大全
  • Bioaggregate Inhibits Osteoclast Differentiation, Fusion, and Bone Resorption In Vitro.

    abstract：INTRODUCTION:Several bioactive bioceramics with osteoconductive effects can inhibit osteoclast formation and bone resorption in vitro, but the exact underlying mechanism has not been fully elucidated. Bioaggregate (BA), a novel calcium silicate nanoparticulate bioceramic, significantly induces bone and periodontal rege...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2015.05.018

    authors： Tian J,Qi W,Zhang Y,Glogauer M,Wang Y,Lai Z,Jiang H

    更新日期：2015-09-01 00:00:00

  • Modeling Hypoxia Induced Factors to Treat Pulpal Inflammation and Drive Regeneration.

    abstract：INTRODUCTION:The ability to resolve pulpal inflammation to achieve predictable regeneration of the dentin-pulp complex has remained elusive and presents a challenge for clinicians and researchers. Although the dentin-pulp complex can react naturally to injury by forming a bridge of reparative dentin that protects the p...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2020.06.039

    authors： Colombo JS,Jia S,D'Souza RN

    更新日期：2020-09-01 00:00:00

  • Nanoparticulates for antibiofilm treatment and effect of aging on its antibacterial activity.

    abstract：INTRODUCTION:Issues pertaining to the effective elimination of bacterial biofilm and disruption of biofilm structure still remains in endodontic disinfection. Nanoparticulates such as chitosan (CS-np) and zinc oxide (ZnO-np) are known to possess significant antibacterial properties. This study aimed to test (1) the eff...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2010.02.008

    authors： Shrestha A,Shi Z,Neoh KG,Kishen A

    更新日期：2010-06-01 00:00:00

  • In vitro evaluation of the cytotoxicity of pure eugenol.

    abstract：:The aim of this study was to assess the cytotoxicity of pure eugenol in an in vitro method by diluting it to various concentrations in alcohol and determining the maximum noncytotoxic concentration. We used solutions of eugenol and ethyl alcohol that are soluble in water in any given proportion. The cytotoxicity of th...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(96)80012-4

    authors： Gerosa R,Borin M,Menegazzi G,Puttini M,Cavalleri G

    更新日期：1996-10-01 00:00:00

  • Lysyl oxidase and the lysyl oxidase-like protein modulate odontoblastic differentiation of human dental pulp cells.

    abstract：INTRODUCTION:The lysyl oxidase (LOX) family is an emerging family of amine oxidases responsible for the formation of collagen fibrils in the extracellular matrix. To date, 5 LOX family genes have been identified in humans, encoding LOX and LOX-like proteins (LOXL, LOXL2, LOXL3, and LOXL4). The goal of this study was to...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2012.03.014

    authors： Kim EC,Lee HJ,Kim Y

    更新日期：2012-06-01 00:00:00

  • An evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria.

    abstract：:The aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: Facultative aerobes-anaerobes: Candida albicans ATCC 10231; Enterococcus faecalis ATCC 29212; Escherichia coli ATCC 25,922; Pseudomonas aeruginosa ATCC 27,85...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(06)81170-2

    authors： D'Arcangelo C,Varvara G,De Fazio P

    更新日期：1999-05-01 00:00:00

  • Inflammatory Myofibroblastic Tumor Mimicking Apical Periodontitis.

    abstract：:Inflammatory myofibroblastic tumors (IMTs) are rare. IMTs of the head and neck occur in all age groups, from neonates to old age, with the highest incidence occurring in childhood and early adulthood. An IMT has been defined as a histologically distinctive lesion of uncertain behavior. This article describes an unusua...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2015.09.006

    authors： Adachi M,Kiho K,Sekine G,Ohta T,Matsubara M,Yoshida T,Katsumata A,Tanuma J,Sumitomo S

    更新日期：2015-12-01 00:00:00

  • Effects of raw material and rotational speed on the cyclic fatigue of ProFile Vortex rotary instruments.

    abstract：INTRODUCTION:This in vitro study compared the cyclic fatigue resistance of ProFile Vortex rotary instruments (DENTSPLY Tulsa Dental, Tulsa, OK) made of two different raw materials: M-Wire and regular superelastic wire (SE-wire) at two different rotational speeds. Two rotary-based Profile Vortex instruments with taper 0...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2010.02.015

    authors： Gao Y,Shotton V,Wilkinson K,Phillips G,Johnson WB

    更新日期：2010-07-01 00:00:00

  • Long-term prognosis of endodontically treated teeth: a retrospective analysis of preoperative factors in molars.

    abstract：INTRODUCTION:Long-term predictability of restored endodontically treated teeth is important for the decision of tooth retention versus extraction and implant placement. The purpose of this study was to validate the hypothesis that preoperative factors can predict the long-term prognosis of molars requiring endodontic a...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2010.10.005

    authors： Setzer FC,Boyer KR,Jeppson JR,Karabucak B,Kim S

    更新日期：2011-01-01 00:00:00

  • Antibacterial activity of 10% carbamide peroxide bleaching agents.

    abstract：:The antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (Nite White, Karisma, and Opalescence) on Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus casei, and Lactobacillus acidophilus was examined. Chlorhexidine (0.2%) was used as a positive control. Test m...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(96)80217-2

    authors： Gurgan S,Bolay S,Alaçam R

    更新日期：1996-07-01 00:00:00

  • Prevalence of Periapical Lesions in Patients with Osteoporosis.

    abstract：INTRODUCTION:Osteoporosis is a major systemic disease that can significantly deteriorate the quality of life of the affected individuals. It is more common in women, particularly after menopause. Osteoporosis may be associated with alterations in oral health. Treatment of osteoporotic patients mainly involves the admin...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2020.10.019

    authors： Katz J,Rotstein I

    更新日期：2021-02-01 00:00:00

  • Adverse response to vital bleaching.

    abstract：:A case study is presented that described an acute flare-up of a tooth following a vital bleaching procedure. The case illustrates (a) the importance of assessing the pulpal and periradicular status of a tooth prior to any vital bleaching procedure; and (b) the alteration of the local adaptation syndrome by the bleachi...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(06)80488-7

    authors： Glickman GN,Frysh H,Baker FL

    更新日期：1992-07-01 00:00:00

  • Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth.

    abstract：:The purpose of this investigation was to compare the antimicrobial effect of MTAD (a mixture of a tetracycline isomer, an acid, and a detergent) with that of NaOCl with and without EDTA. Eighty-five extracted human teeth were contaminated with Enterococcus faecalis for 4 weeks. After biomechanical instrumentation usin...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200309000-00008

    authors： Shabahang S,Torabinejad M

    更新日期：2003-09-01 00:00:00

  • Effect of apical size and taper on volume of irrigant delivered at working length with apical negative pressure at different root curvatures.

    abstract：INTRODUCTION:The purpose of this investigation was to determine the effect that apical preparation size and preparation taper have on the volume of irrigant delivered at the working length for different canal curvatures using apical negative pressure irrigation. METHODS:One hundred fifty-five human teeth (55 maxillary...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joen.2012.10.008

    authors： de Gregorio C,Arias A,Navarrete N,Del Rio V,Oltra E,Cohenca N

    更新日期：2013-01-01 00:00:00

  • Solubility of Epiphany endodontic sealer prepared with resinous solvent.

    abstract：PURPOSE:To evaluate in vitro the solubility of the Epiphany endodontic filling material Epiphany (Pentron Clinical Technologies, Wallingford, CT) prepared with its resinous solvent. METHODS:The specimens were prepared in the following experimental conditions: (1) GI, epiphany without photoactivation; (2) GII, Epiphany...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2009.01.021

    authors： Mathias-Junior O,Souza-Gabriel AE,Saraiva Miranda CE,Pécora JD,Correa Silva-Sousa YT,Sousa-Neto MD

    更新日期：2009-05-01 00:00:00

  • Calcium Hydroxide versus Mineral Trioxide Aggregate for Direct Pulp Capping: A Cost-effectiveness Analysis.

    abstract：INTRODUCTION:Recent evidence finds mineral trioxide aggregate (MTA) more effective than calcium hydroxide (CH) for direct pulp capping (DPC). The present study assessed the cost-effectiveness of MTA versus CH for DPC using a model-based simulation approach. METHODS:A mixed public/private payer perspective in the conte...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2015.08.019

    authors： Schwendicke F,Brouwer F,Stolpe M

    更新日期：2015-12-01 00:00:00

  • Preliminary evaluation of bioactive glass S53P4 as an endodontic medication in vitro.

    abstract：:Calcium hydroxide is the "gold standard" endodontic medication, but it may fail to eliminate certain facultative bacteria and yeasts found in root canal systems. In this study, standardized bovine dentin blocks infected with Enterococcus faecalis were treated with an aqueous calcium hydroxide or bioactive glass S53P4 ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200404000-00009

    authors： Zehnder M,Söderling E,Salonen J,Waltimo T

    更新日期：2004-04-01 00:00:00

  • Anesthetic Efficacy of a Combination of 4% Prilocaine/2% Lidocaine with Epinephrine for the Inferior Alveolar Nerve Block: A Prospective, Randomized, Double-blind Study.

    abstract：INTRODUCTION:Prilocaine plain has a high pH and concentration (4%), which could decrease the pain of injection and increase success. The purpose of this study was to compare pain associated with anesthetic solution deposition and the degree of pulpal anesthesia obtained with the combination of prilocaine and lidocaine ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joen.2018.01.004

    authors： Cook O,Nusstein J,Drum M,Fowler S,Reader A,Draper J

    更新日期：2018-05-01 00:00:00

  • Resistance to fracture of roots obturated with novel canal-filling systems.

    abstract：INTRODUCTION:This study was conducted to evaluate and compare the fracture resistance of roots obturated with various contemporary canal-filling systems. METHODS:Fifty single-rooted extracted mandibular premolars were decoronated to obtain 13-mm root segments. Ten roots were left unprepared and unfilled (negative cont...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2011.08.008

    authors： Ghoneim AG,Lutfy RA,Sabet NE,Fayyad DM

    更新日期：2011-11-01 00:00:00

  • Morphologic Change of Parvalbumin-positive Myelinated Axons in the Human Dental Pulp.

    abstract：INTRODUCTION:Information on the nerve fibers innervating the dental pulp is crucial for understanding dental pain and hypersensitivity. This study investigated the morphologic differences of parvalbumin (PV)-positive (+) myelinated fibers in 3 different regions of the human dental pulp. METHODS:Light and electron micr...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2017.01.010

    authors： Kim TH,Park SK,Choi SY,Lee JS,Bae YC

    更新日期：2017-06-01 00:00:00

  • Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population.

    abstract：:The occurrence of Enterococcus faecalis in root canals of previously root filled teeth with apical periodontitis requiring retreatment was studied in Lithuanian patients. Twenty-five asymptomatic teeth were included in the study. Avoiding contamination microbiological samples were taken from the canals before and afte...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200010000-00004

    authors： Peciuliene V,Balciuniene I,Eriksen HM,Haapasalo M

    更新日期：2000-10-01 00:00:00

  • Biomimetic approach to perforation repair using dental pulp stem cells and dentin matrix protein 1.

    abstract：INTRODUCTION:Dentin regeneration could be an ideal treatment option to restore tissue function. This study was conducted to evaluate the ability of dental pulp stem cells (DPSCs) and dentin matrix protein 1 (DMP1) impregnated within a collagen scaffold to regenerate dentin. METHODS:Simulated perforations were created ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2011.05.019

    authors： Alsanea R,Ravindran S,Fayad MI,Johnson BR,Wenckus CS,Hao J,George A

    更新日期：2011-08-01 00:00:00

  • Cone-beam Computed Tomography for Detecting Vertical Root Fractures in Endodontically Treated Teeth: A Systematic Review.

    abstract：INTRODUCTION:A vertical root fracture (VRF), commonly found in teeth with endodontic treatment, is challenging to diagnose and has poor treatment outcomes. Cone-beam computed tomography (CBCT) has become an increasingly popular imaging modality in endodontics, but image artifacts arising from root-filling materials may...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,评审

    doi：10.1016/j.joen.2015.10.005

    authors： Chang E,Lam E,Shah P,Azarpazhooh A

    更新日期：2016-02-01 00:00:00

  • Anatomical relationship of the mandibular canal to its surrounding structures in mature mandibles.

    abstract：:A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(06)81411-1

    authors： Denio D,Torabinejad M,Bakland LK

    更新日期：1992-04-01 00:00:00

  • Validity of Preoperative Clinical Findings to Identify Dental Pulp Status: A National Dental Practice-Based Research Network Study.

    abstract：INTRODUCTION:Endodontic diagnostic tests are often used clinically to assess pulp status as a basis for the diagnosis and determination of whether root canal treatment (RCT) is indicated. Response to cold and pain on percussion are 2 common tests, yet their validity in identifying nonvital pulp in regular dental practi...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2016.03.016

    authors： Pigg M,Nixdorf DR,Nguyen RH,Law AS,National Dental Practice-Based Research Network Collaborative Group.

    更新日期：2016-06-01 00:00:00

  • Root strains associated with different obturation techniques.

    abstract：:The influence of different obturation techniques (lateral condensation, Obtura, and Thermafil) on root strains was assessed in 27 upper central incisors using strain gauges mounted on the coronal and apical one-thirds of the root surface. The vertical load applied with each obturation technique was also measured. Comp...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(06)81008-3

    authors： Saw LH,Messer HH

    更新日期：1995-06-01 00:00:00

  • Outcomes of Direct Pulp Capping by Using Either ProRoot Mineral Trioxide Aggregate or Biodentine in Permanent Teeth with Carious Pulp Exposure in 6- to 18-Year-Old Patients: A Randomized Controlled Trial.

    abstract：INTRODUCTION:This study aimed to compare the success rates of direct pulp capping (DPC) by using either ProRoot Mineral Trioxide Aggregate (MTA) or Biodentine in the cariously exposed permanent teeth of 6- to 18-year-old patients. Gray discoloration was also evaluated. METHODS:Fifty-nine cariously exposed permanent te...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joen.2017.10.012

    authors： Parinyaprom N,Nirunsittirat A,Chuveera P,Na Lampang S,Srisuwan T,Sastraruji T,Bua-On P,Simprasert S,Khoipanich I,Sutharaphan T,Theppimarn S,Ue-Srichai N,Tangtrakooljaroen W,Chompu-Inwai P

    更新日期：2018-03-01 00:00:00

  • Leakage along apical root fillings in curved root canals. Part I: effects of apical transportation on seal of root fillings.

    abstract：:Occurrence of apical transportation (AT) may complicate the root filling procedure and result in a compromised seal. In part I of this study, human mandibular premolars with single, curved (21 to 39 degrees), or straight canals were prepared by Lightspeed or a step-back hand filing technique. An AT index was determine...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200004000-00003

    authors： Wu MK,Fan B,Wesselink PR

    更新日期：2000-04-01 00:00:00

  • The role of integrin-α5 in the proliferation and odontogenic differentiation of human dental pulp stem cells.

    abstract：INTRODUCTION:It has been reported that integrin-α5 (ITGA5) activity is related to cell proliferation, differentiation, migration, and organ development. However, the involvement of ITGA5 in the biological functions of human dental pulp stem cells (hDPSCs) has not been explored. The aim of this study was to investigate ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2013.08.011

    authors： Cui L,Xu S,Ma D,Gao J,Liu Y,Yue J,Wu B

    更新日期：2014-02-01 00:00:00

  • Disinfecting and Shaping Type I C-shaped Root Canals: A Correlative Micro-computed Tomographic and Molecular Microbiology Study.

    abstract：INTRODUCTION:This ex vivo study compared the disinfection and shaping abilities of 2 preparation protocols in C-shaped canals. METHODS:Mandibular second molars with type I C-shaped canals were pair matched based on micro-computed tomographic (micro-CT) analysis and distributed into 2 groups. The canals were contaminat...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2020.11.007

    authors： Gazzaneo I,Amoroso-Silva P,Pacheco-Yanes J,Alves FRF,Marceliano-Alves M,Olivares P,Meto A,Mdala I,Siqueira JF Jr,Rôças IN

    更新日期：2020-11-18 00:00:00