Abstract：
INTRODUCTION:The lysyl oxidase (LOX) family is an emerging family of amine oxidases responsible for the formation of collagen fibrils in the extracellular matrix. To date, 5 LOX family genes have been identified in humans, encoding LOX and LOX-like proteins (LOXL, LOXL2, LOXL3, and LOXL4). The goal of this study was to evaluate the expression and function of the LOX family genes in odontoblastic differentiation of human dental pulp (HDP) cells. METHODS:Expression of the LOX family genes was assessed by reverse transcriptase polymerase chain reaction analysis, and the amine oxidase activity of HDP cells was evaluated by peroxidase-coupled fluorometric assays. Mineral nodule formation and expression of odontoblastic marker genes were assessed in the presence and absence of specific small interfering RNAs (siRNAs) of the LOX family genes. RESULTS:Among the LOX family genes, only LOX and LOXL showed prominent expression during odontoblastic differentiation of HDP cells. Suppression of LOX and LOXL expression by siRNA-induced interference substantially decreased the amine oxidase activity of the differentiating HDP cells. Furthermore, interference of LOX and LOXL expression inhibited mineral nodule formation and expression of odontoblastic marker genes during odontoblastic differentiation of HDP cells. CONCLUSIONS:These findings show for the first time that the LOX- and LOXL-mediated organization of collagen fibrils in extracellular matrices of HDP cells might be an important regulator for odontoblastic differentiation of HDP cells.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Kim EC,Lee HJ,Kim Ydoi
10.1016/j.joen.2012.03.014subject
Has Abstractpub_date
2012-06-01 00:00:00pages
769-73issue
6eissn
0099-2399issn
1878-3554pii
S0099-2399(12)00295-6journal_volume
38pub_type
杂志文章abstract：INTRODUCTION:This in vitro study compared cone-beam computed tomography (CBCT) exam with different voxel sizes with digital periapical radiography in the detection of vertical root fractures in teeth with and without intracanal metallic posts. METHODS:Eighteen single-rooted human teeth were endodontically treated, pre...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.031
更新日期：2013-12-01 00:00:00
abstract：INTRODUCTION:The direct effect of follicle-stimulating hormone (FSH) on bone resorption has been demonstrated recently. The aim of this study was to evaluate the effect of FSH inhibitor leuprorelin (LE) on alveolar bone loss resulting from experimental periapical lesions in ovariectomized (OVX) rats. METHODS:Twenty-se...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.01.011
更新日期：2010-04-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to determine the variations in root canal length (RCL) occurring during endodontic treatment stages (initial, preflared, and concluded) and correlate them with the accuracy of Root ZX II (RZX). METHODS:After coronal access, 26 mandibular molars had the apical foramen of the 52 me...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.07.022
更新日期：2016-11-01 00:00:00
abstract：INTRODUCTION:Interferon regulatory factor 8 (IRF8) is a critical transcription factor in innate immune responses that regulates the development and function of myeloid cells. Human periapical lesions are caused by endodontic microbial infections. However, the presence of IRF8 in human periapical lesions remains elusive...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.04.015
更新日期：2018-08-01 00:00:00
abstract：:Silicone tubes filled with CRCS, a calcium hydroxide-based cement, were implanted at two different localizations in the right tibias of white male Wistar rats. A similar number of solid silicone rods were implanted in the left tibias of the same animals and were used as inert controls. Fifteen days after implantation ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80090-1
更新日期：1989-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the influence of anorganic bovine bone as a grafted biomaterial on newly formed bone and cementum in periapical regions after surgical endodontic treatment in cats. METHODS:After inducing apical periodontitis in 9 cats, root canal and surgical endodontic treatment...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.10.002
更新日期：2012-02-01 00:00:00
abstract：:The mesiobuccal roots of 50 randomly selected maxillary first molars were examined to assess the incidence and position of the canal isthmus. Transverse serial sections of the apical 6 mm of each root were prepared in 1-mm increments. The apical side of each section was stained with methylene blue dye, viewed with a s...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80975-1
更新日期：1995-07-01 00:00:00
abstract：:The endodontic endosseous implant (EEI) enjoyed wide use in the United States after it was introduced in the 1960's. For various reasons including incorrect case selection, improper use of the materials, and poor preparation for the implant a high number of failures resulted. Following opposition by some members of th...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81496-2
更新日期：1993-10-01 00:00:00
abstract：:Biofilm mode of growth is a strategy in microorganisms to survive harsh growth conditions. Although previous studies have established the ability of Enterococcus faecalis to survive postendodontic environmental conditions, the effect of such conditions on the ultrastructural and physiochemical features of E. faecalis ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000164855.98346.fc
更新日期：2005-12-01 00:00:00
abstract：:Pulp regeneration is a biologic process occurring under specific circumstances. An endodontic treatment modality to accomplish pulp regeneration has emerged based on the response of undifferentiated cells that are often referred to as stem cells. The treatment itself is currently empirical based on clinicians' thought...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2014.01.017
更新日期：2014-04-01 00:00:00
abstract：INTRODUCTION:There are no long-term, prospective clinical studies assessing outcomes of endodontically treated cracked teeth with radicular extensions. The purpose of this prospective study was to examine the 2- to 4-year success and survival rates of endodontically treated, coronally restored, cracked teeth, specifica...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.015
更新日期：2019-07-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate and compare the centering ability and canal transportation of the ProTaper Next (PTN; Dentsply Maillefer, Ballaigues, Switzerland) and Self-adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) systems in long oval root canals using cone-beam computed tomography imag...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.07.007
更新日期：2019-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effect of chitosan-hydroxyapatite precursor (C-HA) nanocomplex conditioning on the chemical modifications at the tricalcium silicate sealer-dentin interface using time-of-flight secondary ion mass spectrometry. METHODS:Dentin slabs from human premolar root den...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.08.011
更新日期：2019-12-01 00:00:00
abstract：INTRODUCTION:The present study aimed to evaluate the antibacterial effect of a new peptide, DJK-5, used alone or mixed together with EDTA on mono- and multispecies biofilms in dentin canals covered by a smear layer with or without preceding sodium hypochlorite (NaOCl) irrigation. METHODS:One hundred twelve dentin bloc...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.018
更新日期：2018-11-01 00:00:00
abstract：:This study compared the effect of two drills and five cleaning regimens on post space debridement. One hundred extracted premolars were instrumented and obturated with warm vertical compaction of gutta percha. The teeth were divided into two groups according to the drill used to remove gutta percha/sealer and for post...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2007.10.019
更新日期：2008-01-01 00:00:00
abstract：:A technique using K files with modified tips has been developed for the penetration of constricted canals. The tip of a #10 K file was sliced diagonally to make it thinner. Because this modified K file has an especially fine tip and an appropriate stiffness, it followed constricted or sharply curved canals well and ha...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(97)80190-2
更新日期：1997-06-01 00:00:00
abstract：:The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.07.002
更新日期：2008-05-01 00:00:00
abstract：:The state of contamination by methicillin-resistant Staphylococci, including methicillin-resistant Staphylococcus aureus (MRSA) at the conservative dentistry section of the School of Dentistry, Aichi-Gakuin University, was investigated. Methicillin-resistant coagulase-negative Staphylococci (MRCNS) were detected in 8 ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80552-2
更新日期：1995-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the durability of insulated probes designed to be used with the Endocater Apex Locator when put through multiple cleaning and autoclaving cycles. The manufacturer has reported that the plastic coating on the probes is reliable with normal use and sterilizable up to a maximum o...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81502-5
更新日期：1993-08-01 00:00:00
abstract：INTRODUCTION:The aim of the present study was to compare the immunoexpression of CD34, intercellular adhesion molecule-1 (ICAM-1), and podoplanin and the presence of mast cells with clinical, demographic, radiologic, and histologic features from periapical granulomas, periapical cysts, and residual cysts. METHODS:Thir...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.04.013
更新日期：2018-07-01 00:00:00
abstract：INTRODUCTION:This study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (MTA) mixed with aqueous and propylene glycol extracts of Arctium lappa L. and Casearia sylvestris Sw. METHODS:The setting-time test was performed according to American Society for Testin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.12.023
更新日期：2017-05-01 00:00:00
abstract：:The denaturation temperature (T(d)) of dentin collagen in mineralized versus demineralized teeth was examined as a function of dentin age and the extent of dehydration. Using differential scanning calorimetry, T(d) of mineralized dentin was shown to be between 160 degrees C to 186 degrees C, depending on whether it wa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.062
更新日期：2006-07-01 00:00:00
abstract：:Orthodontic tooth movement has been implicated in secondary changes to the dental pulp. The purpose of this study was to correlate the effects of orthodontic tooth movement on the dental pulp by histomorphometric parameters. Four groups, each consisting of 36 male adult Sprague-Dawley strain rats, were studied with di...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81034-4
更新日期：1993-01-01 00:00:00
abstract：INTRODUCTION:A 3-antibiotic combination (3Mix) has been widely used in regenerative endodontics. Recent studies recommend that a safe concentration of 3Mix is in the range of 0.39 μg/mL and 1 mg/mL because higher concentrations may limit tissue regeneration. The aim of this study was to determine the regenerative capac...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.09.027
更新日期：2014-03-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to assess temperature changes in conventional gutta-percha, thermoplastic gutta-percha, and Resilon cones by using thermomechanical compaction technique. METHODS:The root canals of extracted human teeth were placed in resin blocks and sectioned. After root canal preparation, cavi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.03.009
更新日期：2009-06-01 00:00:00
abstract：INTRODUCTION:Collagen dentin matrix may represent a suitable scaffold to be remineralized in the presence of bioactive materials. The purpose of this study was to determine if experimental resin cements containing bioactive fillers may modulate matrix metalloproteinase-mediated collagen degradation of etched dentin. M...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.05.011
更新日期：2012-09-01 00:00:00
abstract：:Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.037
更新日期：2006-03-01 00:00:00
abstract：:Ninety-six human molars were accessed. After flattening of the occlusal surface and removal of the apical two-thirds of the roots, the remaining canal space was instrumented by conventional methods. The teeth were divided into six groups of 15 teeth with three positive and three negative controls. The six experimental...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80078-6
更新日期：1994-12-01 00:00:00
abstract：:The antimicrobial effect of RC-Prep and its components--10% urea peroxide, 15% EDTA, and glycol--were evaluated individually, in vitro, within dentinal tubules. Standardized bovine incisor root specimens that had been infected with either Staphylococcus aureus or Streptococcus peltzer were incubated with each agent fo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80296-9
更新日期：1999-12-01 00:00:00
abstract：:In the past, measurement of microleakage has been based on measurement of dye or radioactive isotope penetration. A method has been developed to allow electronic monitoring of microflow in the same specimen over a period of time. This method was used to compare previously reported retrograde materials with an amalgam/...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80072-5
更新日期：1994-11-01 00:00:00
