Real-time quantitative polymerase chain reaction and culture analyses of Enterococcus faecalis in root canals.

Abstract:

:Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal sample. Consecutive root canal samples obtained from primary infection (n = 40) and retreatment (n = 48) cases were divided into two equal aliquots that were independently analyzed using culture and qPCR by investigators blinded to the analysis results of the other sample. E. faecalis was detected in 10.2% and 79.5% of samples by culture and qPCR, respectively (p < 0.0001; McNemar's test). E. faecalis was detected in more retreatment than primary infection samples (89.6% versus 67.5%; p = 0.01, Fisher's exact test). qPCR reported a significantly higher prevalence of E. faecalis in endodontic samples than culture techniques.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Sedgley C,Nagel A,Dahlén G,Reit C,Molander A

doi

10.1016/j.joen.2005.10.037

keywords:

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

173-7

issue

3

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)00077-4

journal_volume

32

pub_type

杂志文章
  • In vitro cytotoxicity of a new epoxy resin root canal sealer.

    abstract::The cytotoxic effects of a new epoxy resin-based root canal sealer (AH-plus), together with those of two other commonly used endodontic sealers (AH26 and zinc oxide-eugenol), have been studied in vitro on a culture of human gingival fibroblasts. Cytotoxicity was assessed by direct incubation of sealers' extracts with ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200008000-00008

    authors: Azar NG,Heidari M,Bahrami ZS,Shokri F

    更新日期:2000-08-01 00:00:00

  • Postorthodontic external root resorption in root-filled teeth is influenced by interleukin-1β polymorphism.

    abstract:INTRODUCTION:External apical root resorption (EARR) is a frequent iatrogenic effect of orthodontic treatment. The way root-filled teeth respond to orthodontic forces with respect to EARR has been reported as varying widely between individuals. Genetic variants in the interleukin-1 gene have been associated with an incr...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2011.12.022

    authors: Iglesias-Linares A,Yañez-Vico RM,Ortiz-Ariza E,Ballesta S,Mendoza-Mendoza A,Perea E,Solano-Reina E

    更新日期:2012-03-01 00:00:00

  • EM evaluation of bacterial biofilm and microorganisms on the apical external root surface of human teeth.

    abstract::The aim of this study was to evaluate the presence of bacterial biofilm on the external surface of the root apex in teeth with pulp necrosis, with and without radiographically visible periapical lesions, and in teeth with a vital pulp. Twenty-one teeth were extracted, eight with pulp necrosis and periapical lesions, e...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200212000-00006

    authors: Leonardo MR,Rossi MA,Silva LA,Ito IY,Bonifácio KC

    更新日期:2002-12-01 00:00:00

  • The effect of antibiotics and endodontic antimicrobials on the polymerase chain reaction.

    abstract::The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tet...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000152899.54247.4e

    authors: Fouad AF,Barry J

    更新日期:2005-07-01 00:00:00

  • Cyclic Fatigue Resistance of Reciproc Blue and Reciproc Files in an S-shaped Canal.

    abstract:INTRODUCTION:This study evaluated the cyclic fatigue resistance (CFR) of Reciproc (R25 and R40; VDW, Munich, Germany) and Reciproc Blue (R25 and R40, VDW) instruments used in an artificial S-shaped canal. METHODS:A total of 80 files were tested in an S-shaped canal (n = 20 for each file, Reciproc R25 and R40 and Recip...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2017.04.009

    authors: Topçuoğlu HS,Topçuoğlu G

    更新日期:2017-10-01 00:00:00

  • Cytotoxicity of newly developed ortho MTA root-end filling materials.

    abstract:INTRODUCTION:Various materials have been advocated for use as root-end filling materials. The purpose of the present in vitro study was to compare the cytotoxicity of 4 root-end filling materials: glass ionomer cement (GIC; Fuji II, GC Corp, Tokyo, Japan), reinforced zinc oxide-eugenol cement (IRM; Dentsply Tulsa Denta...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2012.09.004

    authors: Lee BN,Son HJ,Noh HJ,Koh JT,Chang HS,Hwang IN,Hwang YC,Oh WM

    更新日期:2012-12-01 00:00:00

  • Prevalence of Candida Species in Endodontic Infections: Systematic Review and Meta-analysis.

    abstract:INTRODUCTION:Candida in endodontic infections has been investigated in a large number of studies, but its role as an endodontic pathogen is still debatable. The aim of this study was to systematically review the literature on the prevalence of Candida species in root canal infections. METHODS:Extensive literature rese...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.joen.2018.07.016

    authors: Mergoni G,Percudani D,Lodi G,Bertani P,Manfredi M

    更新日期:2018-11-01 00:00:00

  • Torsional Performance of ProTaper Gold Rotary Instruments during Shaping of Small Root Canals after 2 Different Glide Path Preparations.

    abstract:INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2016.10.034

    authors: Arias A,de Vasconcelos RA,Hernández A,Peters OA

    更新日期:2017-03-01 00:00:00

  • Evaluation of ultrasonic and sonic instruments for intraradicular post removal.

    abstract::Ultrasonic instruments are a valuable asset for removing intraradicular posts from root canals before nonsurgical endodontic therapy. The purpose of this study was to evaluate the relative efficacy of post removal by ultrasonic and sonic devices. Fifty extracted teeth were instrumented and obturated. The canals were p...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)80044-0

    authors: Buoncristiani J,Seto BG,Caputo AA

    更新日期:1994-10-01 00:00:00

  • DIAGNOdent laser fluorescence assessment of endodontic infection.

    abstract:INTRODUCTION:Real-time assessment of the microbial status of the root canal system would be useful in clinical endodontic practice for determining endpoints of biomechanical treatment. This laboratory study used an existing laser fluorescence device, the DIAGNOdent (KaVo, Biberach, Germany), in a proof-of-concept study...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2009.07.006

    authors: Sainsbury AL,Bird PS,Walsh LJ

    更新日期:2009-10-01 00:00:00

  • Pulp-dentine complex changes and root resorption during intrusive orthodontic tooth movement in patients prescribed nabumetone.

    abstract::Pulpitis, external root resorption, and pain may be experienced during orthodontic movement. The use of nonsteroidal anti-inflammatory drugs (NSAIDs) has been suggested to control these changes. The purpose of this study was to observe pulp-dentinal reactions, root resorption, tooth pain, and tooth movement after the ...

    journal_title:Journal of endodontics

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/01.don.0000134212.20525.74

    authors: Villa PA,Oberti G,Moncada CA,Vasseur O,Jaramillo A,Tobón D,Agudelo JA

    更新日期:2005-01-01 00:00:00

  • Antimicrobial Efficacy of Chlorhexidine and Sodium Hypochlorite in Root Canal Disinfection: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

    abstract:INTRODUCTION:We aimed to compare the antimicrobial efficacy of chlorhexidine (CHX) and sodium hypochlorite (NaOCl), 2 irrigants routinely used in root canal therapy of permanent teeth. METHODS:Electronic databases, including PubMed, EMBASE, Web of Science, and Cochrane Library, were searched for randomized controlled ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.joen.2020.05.002

    authors: Ruksakiet K,Hanák L,Farkas N,Hegyi P,Sadaeng W,Czumbel LM,Sang-Ngoen T,Garami A,Mikó A,Varga G,Lohinai Z

    更新日期:2020-08-01 00:00:00

  • Cracked Teeth and Poor Oral Masticatory Habits: A Matched Case-control Study in China.

    abstract:INTRODUCTION:Previous studies have suggested that masticatory forces are associated with cracked teeth, but it is not clear which specific poor oral masticatory habits may be more likely to cause cracks. The aim of this study was to quantitatively assess risk factors for cracked teeth among poor oral masticatory habits...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2017.01.007

    authors: Qiao F,Chen M,Hu X,Niu K,Zhang X,Li Y,Wu Z,Shen Z,Wu L

    更新日期:2017-06-01 00:00:00

  • Endodontic implications of bisphosphonate-associated osteonecrosis of the jaws: a report of three cases.

    abstract::Bisphosphonates are commonly used in medicine to maintain bone density in patients with certain nonneoplastic diseases or cancers. A serious adverse effect of bisphosphonates that has substantial dental significance is osteonecrosis that appears to uniquely affect the mandible and maxilla without occurring in other bo...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000186481.96169.cd

    authors: Katz H

    更新日期:2005-11-01 00:00:00

  • EDTA Promotes the Mineralization of Dental Pulp In Vitro and In Vivo.

    abstract:INTRODUCTION:Dentin regeneration is one of the main goals of vital pulp treatment in which the biological properties of dental pulp cells (DPCs) need to be considered. In our previous study, we showed that EDTA could enhance the stromal cell-derived factor 1 alpha-induced migration of DPCs. The purpose of this study wa...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.12.003

    authors: Liu L,Leng S,Tang L,Lu Q,Xu W,Tan X,Huang D,Zhang L

    更新日期:2020-12-20 00:00:00

  • Occurrence of Vertical Root Fractures after Apical Surgery: A Retrospective Analysis.

    abstract:INTRODUCTION:Vertical root fractures (VRFs) are among the most frequent causes of tooth loss, mainly of endodontically treated teeth. However, very few data is available about the occurrence of VRFs following apical surgery. METHODS:Patient charts from 864 patients with 1058 teeth treated with apical surgery (Septembe...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.10.012

    authors: von Arx T,Maldonado P,Bornstein MM

    更新日期:2021-02-01 00:00:00

  • Depth of penetration of four resin sealers into radicular dentinal tubules: a confocal microscopic study.

    abstract:BACKGROUND:The aim of this in vitro study was to evaluate the depth of penetration of 4 different endodontic resin sealers into the radicular dentinal tubules with the aid of confocal microscopy. METHODS:Eighty single-rooted teeth were instrumented and divided into 4 groups composed of 20 teeth each. The samples were ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2012.05.017

    authors: Chandra SS,Shankar P,Indira R

    更新日期:2012-10-01 00:00:00

  • Prevalence of Periapical Lesions in Patients with Osteoporosis.

    abstract:INTRODUCTION:Osteoporosis is a major systemic disease that can significantly deteriorate the quality of life of the affected individuals. It is more common in women, particularly after menopause. Osteoporosis may be associated with alterations in oral health. Treatment of osteoporotic patients mainly involves the admin...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.10.019

    authors: Katz J,Rotstein I

    更新日期:2021-02-01 00:00:00

  • Evaluation of Biocompatibility and Osteogenic Potential of Tricalcium Silicate-based Cements Using Human Bone Marrow-derived Mesenchymal Stem Cells.

    abstract:INTRODUCTION:The success of endodontic regeneration lies in the appropriate combination of stem cells and bioactive materials. Several novel dental materials are available on the market in this regard. Hence, the current study aimed to evaluate the proliferation, differentiation, and osteogenic potential of human bone ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2017.11.016

    authors: Sultana N,Singh M,Nawal RR,Chaudhry S,Yadav S,Mohanty S,Talwar S

    更新日期:2018-03-01 00:00:00

  • Effect of varying water-to-powder ratios on the setting expansion of white and gray mineral trioxide aggregate.

    abstract:INTRODUCTION:Clinicians commonly mix mineral trioxide aggregate (MTA) to a desired consistency rather than use the recommended amounts of powder and water. The purpose of this in vitro study was to evaluate how varying the water-to-powder (WP) ratio affects the setting expansion of white MTA (WMTA) and gray MTA (GMTA)....

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2010.03.010

    authors: Hawley M,Webb TD,Goodell GG

    更新日期:2010-08-01 00:00:00

  • Delayed partial pulpotomy in a midroot and complicated crown-root-fractured permanent incisor with hyperplastic pulpitis: a case report.

    abstract:INTRODUCTION:The occurrence of multiple injuries of the same tooth is extremely rare. This case report describes the treatment in a midroot and complicated crown-root-fractured permanent incisor that remained untreated for 4 years after the accident. METHODS:The tooth was treated by delayed partial pulpotomy using cal...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2010.03.036

    authors: Calişkan MK,Savranoğlu S

    更新日期:2010-07-01 00:00:00

  • An ex vivo comparison of digital radiography and cone-beam and micro computed tomography in the detection of the number of canals in the mesiobuccal roots of maxillary molars.

    abstract:INTRODUCTION:The purpose of this study was to compare digital periapical and cone-beam computed tomography (CBCT) images to determine the number of canals in the mesiobuccal root of maxillary molars and to compare these counts with micro computed tomography (μCT), which was also used to determine canal configuration. ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.01.010

    authors: Domark JD,Hatton JF,Benison RP,Hildebolt CF

    更新日期:2013-07-01 00:00:00

  • Determination of lidocaine in dental pulp by high-performance liquid chromatography.

    abstract::The purpose of this study was to develop a method for lidocaine detection in dental pulp by high-performance liquid chromatography. The amounts of lidocaine in dog pulps were quantitated after local injection to evaluate lidocaine recovery from pulp tissue with this technique. Comparison was also made between the amou...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200101000-00009

    authors: Kanjanawattana S,Mangkornkarn C,Wilairat P,Vongsavan N

    更新日期:2001-01-01 00:00:00

  • Effect of guided tissue regeneration on newly formed bone and cementum in periapical tissue healing after endodontic surgery: an in vivo study in the cat.

    abstract:INTRODUCTION:The purpose of this study was to evaluate the influence of anorganic bovine bone as a grafted biomaterial on newly formed bone and cementum in periapical regions after surgical endodontic treatment in cats. METHODS:After inducing apical periodontitis in 9 cats, root canal and surgical endodontic treatment...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2011.10.002

    authors: Artzi Z,Wasersprung N,Weinreb M,Steigmann M,Prasad HS,Tsesis I

    更新日期:2012-02-01 00:00:00

  • Influence of single-file endodontics on apical transportation in curved root canals: an ex vivo micro-computed tomographic study.

    abstract:INTRODUCTION:More information regarding the ability of single files to maintain the original canal position is required before adopting a single-file approach to cleaning and shaping. This study compares apical transportation in curved root canals when instrumenting with a single WaveOne (WO) file (Dentsply Tulsa Denta...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.09.021

    authors: Junaid A,Freire LG,da Silveira Bueno CE,Mello I,Cunha RS

    更新日期:2014-05-01 00:00:00

  • Effects of sodium tri- and hexametaphosphate on proliferation, differentiation, and angiogenic potential of human dental pulp cells.

    abstract:INTRODUCTION:This study aimed to investigate the effects of 2 inorganic polyphosphates (poly[P]) are linear polymers of orthophosphate (Pi) residues linked by energy-rich phosphoanhydride poly(P) compounds, sodium triphosphate (STP, Na5P3O10) and sodium hexametaphosphate (SHMP, Na15P13O40 ∼ Na20P18O40) on the prolifera...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.01.038

    authors: Bae WJ,Jue SS,Kim SY,Moon JH,Kim EC

    更新日期:2015-06-01 00:00:00

  • Terrein reduces pulpal inflammation in human dental pulp cells.

    abstract::Terrein is a bioactive fungal metabolite whose anti-inflammatory properties are virtually unknown. The purpose of this study was to determine the effects of terrein on lipopolysaccharide (LPS)-induced expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human denta...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2008.01.015

    authors: Lee JC,Yu MK,Lee R,Lee YH,Jeon JG,Lee MH,Jhee EC,Yoo ID,Yi HK

    更新日期:2008-04-01 00:00:00

  • The use of cone-beam computed tomography and digital periapical radiographs to diagnose root perforations.

    abstract:INTRODUCTION:The aim of this study was to compare the sensitivity and specificity of cone-beam computed tomography (CBCT) scans and digital periapical radiographs (PR) in detecting strip and root perforations after root canal treatment in mandibular molars. METHODS:Forty-five curved mesial roots were endodontically pr...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2010.12.003

    authors: Shemesh H,Cristescu RC,Wesselink PR,Wu MK

    更新日期:2011-04-01 00:00:00

  • MTA obturation of pulpless teeth with open apices: bacterial leakage as detected by polymerase chain reaction assay.

    abstract::Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde a...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000128749.50151.24

    authors: de Leimburg ML,Angeretti A,Ceruti P,Lendini M,Pasqualini D,Berutti E

    更新日期:2004-12-01 00:00:00

  • Porphyromonas (Bacteroides) endodontalis: its role in endodontal infections.

    abstract::Porphyromonas endodontalis (formerly Bacteroides endodontalis) is a black-pigmented anaerobic Gram-negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. The presence of P. endodontalis in infected dental root can...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80843-5

    authors: van Winkelhoff AJ,van Steenbergen TJ,de Graaff J

    更新日期:1992-09-01 00:00:00